FlyBase Allele Report: Dmel\Cyt-b5[k09716]

General Information

Symbol

Dmel\Cyt-b5^k09716

Species

D. melanogaster

Name

FlyBase ID

FBal0045504

Feature type

allele

Associated gene

Dmel\Cyt-b5

Associated Insertion(s)

P{lacW}Cyt-b5^k09716

Carried in Construct

Also Known As

dpld^MLB

Key Links

Genomic Maps

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Allele class

hypomorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - molecular evidence

(Kleinhesselink et al., 2011)

Mutagen

P-element activity

(Rodriguez et al., 1996, Wu et al., 1996)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:7,533,546..7,533,546

(Kleinhesselink et al., 2011, Kimbrell, 1996.4.28, Rodriguez et al., 1996, BDGP Project Members, 1994-1999)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Nature of the lesion

Statement

Reference

Insertion within the 5' UTR of Cyt-b5, at position -65 relative to the translation start codon.

(Kleinhesselink et al., 2011)

Insertion components

P{lacW}Cyt-b5^k09716

Product class / Tool use(s)

Encoded product / tool

Tagged with

nuclear protein localization tag

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile

(Kleinhesselink et al., 2011)

female sterile (with Cyt-b5^k14202)

(Kleinhesselink et al., 2011)

male sterile

(Kleinhesselink et al., 2011)

male sterile (with Cyt-b5^k14202)

(Kleinhesselink et al., 2011)

melanotic mass phenotype (with Cyt-b5^k14202)

(Kleinhesselink et al., 2011)

viable

(Kimbrell, 1996.4.28, Rodriguez et al., 1996)

Phenotype Manifest In

lamellocyte (with Cyt-b5^k14202)

(Kleinhesselink et al., 2011)

Detailed Description

Statement

Reference

Homozygous and Cyt-b5^k14202/Cyt-b5^k09716 females and males produce few progeny, even when mated with wild-type flies, and the mutant females lay few eggs.

Cyt-b5^k14202/Cyt-b5^k09716 larvae contain large numbers of circulating lamellocytes (these are not found in normal larvae). Lamellocytes are more prevalent in larvae containing small melanotic tumours, while plasmatocytes are more abundant in larvae containing large melanotic tumours.

(Kleinhesselink et al., 2011)

All larvae and adults exhibit melanotic tumour formation. Adult tumours are in the abdomen and occasionally in the head and thorax.

(Rodriguez et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Cyt-b5^k09716/Cyt-b5^k14202 is rescued by Cyt-b5^UAS.cKa/Scer\GAL4^He.PZ

(Kleinhesselink et al., 2011)

Not rescued by

Cyt-b5^k09716/Cyt-b5^k14202 is not rescued by Cyt-b5^UAS.cKa/Scer\GAL4^Sgs3.PD

(Kleinhesselink et al., 2011)

Comments

The melanotic tumour phenotype seen in Cyt-b5^k14202/Cyt-b5^k09716 animals is rescued by expression of Cyt-b5^Scer\UAS.cKa under the control of Scer\GAL4^He.PZ, but is not rescued when expression is driven by Scer\GAL4^Sgs3.PD.

(Kleinhesselink et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

This allele was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was discarded from the gene disruption project prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Spradling (spradling@mail1.ciwemb.edu).

Complements: Aldh-III⁰³⁶¹⁰.

(BDGP Project Members, 1994-1999)

Level of Ecol\lacZ expression and viability of adults is not altered by bacterial infection of adults.

(Rodriguez et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

97/16

(Rodriguez et al., 1996)

Cyt-b5^k09716

dpld^MLB

(Kleinhesselink et al., 2011, Kimbrell, 1996.4.28, Rodriguez et al., 1996)

dpld^k09716

Name Synonyms

Secondary FlyBase IDs

References (5)

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