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General Information
Symbol
Dmel\grh370
Species
D. melanogaster
Name
FlyBase ID
FBal0045816
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

C17828004T

Amino acid change:

Q821term | grh-PJ; Q821term | grh-PL; Q217term | grh-PN; Q217term | grh-PO

Comment:

The grh370 mutation affects only the neuroblast-specific forms of the protein

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Nucleotide substitution C to T, resulting in the amino acid replacement: Gln to a stop codon, in exon 5.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

grhB37/grh370 mutants do not have surviving doublesex-expressing larval terminal neuroblasts in the terminal region of the female third instar larval ventral nerve cord, similar to controls.

At 96 hours, 65% of grh370 type I neuroblasts are smaller than normal.

In grh370/Df(2R)Pcl7B larvae have 1-2 extra ventral clusters of post-embryonic neuroblasts per hemisegment in the abdomen and the clusters themselves have more cells. In contrast, there are less post-embryonic neuroblasts in the thoracic segments of these larvae. This is reflected at mid-third instar by an increase in mitotic cells per neuroblast cluster in the abdomen and a decrease in mitotic cells per neuroblast cluster in the thorax.

Neural proliferation is reduced in the thorax of grh370/Df(2R)Pcl7B mid-L3 larvae with each neuroblast lineage reduced from an average of 7.1 cells in wild-type larvae incorporating BrdU during the 72-78 hours to 5.3 cells in grh370/Df(2R)Pcl7B mutants. In contrast, neural proliferation in the central abdomen is increased with cells still dividing in grh370/Df(2R)Pcl7B abdomens after the 72-hour time point when division has stopped and apoptosis has occurred in wild type. grh370/Df(2R)Pcl7B ventromedial and ventrolateral neuroblast lineages show a 3.5-fold increase in size compared to the wild-type average. These mutants also generate some supernumerary postembryonic lineages that occupy ectopic positions, often close to the midline.

Homozygous animals survive beyond embryogenesis. Most hemizygotes die during the third larval instar stage, some survive to pupation and 1-2% eclose as adults which have persistently shaking appendages and die shortly after eclosion.

Lethality acts in the larval/pupal stages, with a few escapers.

Virtually identical to grhIM in phenotype: degenerating head skeleton. Cuticle susceptible to rupture.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement
Reference
Phenotype Manifest In
Suppressor of
Statement
Reference

grh370 is a suppressor of larval abdomen & neuroblast phenotype of abd-Ahs.PS

Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
References (14)