At stage 11 expression of l(1)scUAS.cHa under the control of Scer\GAL4da.G32 does not lead to any changes in neuroblast numbers when compared to control embryos.
Before delamination of the SI neuroblasts, the ventral neuroectoderm (VNE) of embryos expressing l(1)scScer\UAS.cHa under the control of Scer\GAL4da.G32 can be subdivided into three longitudinal regions based on average cell size, as in wild-type embryos. More enlarged cells are detected in all regions of the VNE compared to wild type; 81% in the medial, 65% in the intermediate and 71% in the lateral region (wild-type numbers are 63%, 20% and 64% respectively). The morphologies and cell sizes of the neuroblasts are altered compared to wild type; whereas in wild-type embryos the delaminating neuroblasts have small apical surfaces and are basally bloated, almost all SI neuroblasts in the VNE of embryos expressing l(1)scScer\UAS.cHa under the control of Scer\GAL4da.G32 are basally and apically bloated. As in wild-type embryos, the neuroectodermal cells that have become enlarged decrease in size during delamination of the SI neuroblasts.
Neural hypoplasia of the CNS and PNS in Df(1)260-1 hemizygous embryos is strongly reduced by Scer\GAL4da.G32-mediated expression of l(1)scScer\UAS.cHa. Scer\GAL4da.G32-mediated expression is not sufficient to promote the development of ectopic nerve cells in wild type embryos, but does cause defects in internal sensory organs.
Ectopic external sense organs develop, macrochaetae, microchaetae and campaniform sensilla, on virtually every part of the body. Extra veins with microchaetae also develop on the wing blade.