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General Information
D. melanogaster
Saccharomyces cerevisiae UAS construct a of Hinz
FlyBase ID
Feature type
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-l'sc, UAS-l''sc
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
Additional Notes
Associated Sequence Data
DNA sequence
Protein sequence
Progenitor genotype
Carried in construct
Nature of the lesion

Expression of l(1)sc cDNA is governed by UAS regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Modifiers Based on Experimental Evidence ( 0 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
Disease-implicated variant(s)
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

macrochaeta & abdominal sternite 1, with Scer\GAL4sca-537.4

macrochaeta & abdominal sternite 2, with Scer\GAL4sca-537.4

macrochaeta & abdominal sternite 3, with Scer\GAL4sca-537.4

macrochaeta & abdominal sternite 4, with Scer\GAL4sca-537.4

macrochaeta & abdominal sternite 5, with Scer\GAL4sca-537.4

macrochaeta & abdominal sternite 6, with Scer\GAL4sca-537.4

macrochaeta & mesothoracic laterotergite, with Scer\GAL4h-540.3

macrochaeta & metathoracic laterotergite, with Scer\GAL4h-540.3

macrochaeta & pronotum, with Scer\GAL4h-540.3

macrochaeta & scutellum, with Scer\GAL4455.2

macrochaeta & scutum, with Scer\GAL4h-540.3

macrochaeta & wing, with Scer\GAL4564.2

microchaeta & mesothoracic laterotergite, with Scer\GAL4h-540.3

microchaeta & metathoracic laterotergite, with Scer\GAL4h-540.3

microchaeta & pronotum, with Scer\GAL4h-540.3

microchaeta & scutum, with Scer\GAL4h-540.3

scutellum & microchaeta, with Scer\GAL4ptc-559.1

sensory mother cell & dorsal mesothoracic disc, with Scer\GAL4ap-md544

Detailed Description

At stage 11 expression of l(1)scUAS.cHa under the control of Scer\GAL4da.G32 does not lead to any changes in neuroblast numbers when compared to control embryos.

When l(1)scScer\UAS.cHa is driven by Scer\GAL4sca-537.4 complete ectopic mechano-sensory bristles are seen.

Before delamination of the SI neuroblasts, the ventral neuroectoderm (VNE) of embryos expressing l(1)scScer\UAS.cHa under the control of Scer\GAL4da.G32 can be subdivided into three longitudinal regions based on average cell size, as in wild-type embryos. More enlarged cells are detected in all regions of the VNE compared to wild type; 81% in the medial, 65% in the intermediate and 71% in the lateral region (wild-type numbers are 63%, 20% and 64% respectively). The morphologies and cell sizes of the neuroblasts are altered compared to wild type; whereas in wild-type embryos the delaminating neuroblasts have small apical surfaces and are basally bloated, almost all SI neuroblasts in the VNE of embryos expressing l(1)scScer\UAS.cHa under the control of Scer\GAL4da.G32 are basally and apically bloated. As in wild-type embryos, the neuroectodermal cells that have become enlarged decrease in size during delamination of the SI neuroblasts.

Flies with l(1)scScer\UAS.cHa under the control of Scer\GAL4ptc-559.1 form multiple ectopic macrochaetae and microchaetae on the scutellum. l(1)scScer\UAS.cHa, when driven by Scer\GAL4ap-md544, creates a pattern of discrete sensory organ precursors randomly dispersed throughout the dorsal half of the wing pouch in the wing disc.

In strain ZB1.4 the activator insertion Scer\GAL4455.2 is combined with l(1)scScer\UAS.cHa causing the development of 10 ectopic scutellar macrochaetes.

Neural hypoplasia of the CNS and PNS in Df(1)260-1 hemizygous embryos is strongly reduced by Scer\GAL4da.G32-mediated expression of l(1)scScer\UAS.cHa. Scer\GAL4da.G32-mediated expression is not sufficient to promote the development of ectopic nerve cells in wild type embryos, but does cause defects in internal sensory organs.

Ectopic external sense organs develop, macrochaetae, microchaetae and campaniform sensilla, on virtually every part of the body. Extra veins with microchaetae also develop on the wing blade.

External Data
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Suppressed by
NOT suppressed by
NOT Suppressor of
Additional Comments
Genetic Interactions

Co-expression of l(1)scScer\UAS.cHa and daScer\UAS.cGa driven by Scer\GAL4da.G32 results in minor muscle and PNS pattern formation defects and in very rare cases, muscle duplication. The addition of l(1)scScer\UAS.cHa and daScer\UAS.cGa does not rescue the mesoderm phenotype of Nint.SH.Scer\UAS driven by Scer\GAL4da.G32.

Some embryos coexpressing l(1)scScer\UAS.cHa and daScer\UAS.cGa under the control of Scer\GAL4da.G32 develop multiple Malpighian tubule tip cells.

Scer\GAL4455.2-mediated expression leads to an increase in the number of scutellar bristles, on average 6 to 7 ectopic bristles can be counted. Phenotype can be suppressed by mutations of da and H and enhanced by mutations of emc, Dl, E(spl), h, mam, eIF-4a, ptc, Hrb27C, l(2)44DEa, Hr46, jumu and N.

Scer\GAL4da.G32-mediated expression of Dl::NΔECN.Scer\UAS generates aneural embryos. Simultaneous expression of daScer\UAS.cGa and l(1)scScer\UAS.cHa does not rescue the phenotype.

One copy of daIIP106 or a deficiency of da can suppress the wing phenotype, the presence of a third copy of da enhances the phenotype.

Xenogenetic Interactions
Complementation and Rescue Data
Images (0)
Stocks (1)
Notes on Origin
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Hinz
Secondary FlyBase IDs
    References (15)