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General Information
Symbol
Dmel\Dhc64C8-1
Species
D. melanogaster
Name
FlyBase ID
FBal0048198
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
dhc8-1
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Genomic Southern blotting revealed no polymorphism between the mutant and wild type chromosome.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

In embryos laid by Dhc64C8-1/Dhc64C6-10 females the force (applied with optical tweezers) required to stall plus end travel of lipid droplets is significantly less than for wild-type embryos. The velocity of plus end droplet travel is significantly reduced in these mutants, especially over long runs (500-1000 nm), as is the mean plus end travel distance. In addition, the frequency of pauses during plus end travel is increased. All these effects are also seen, although more weakly, in embryos from Dhc64C8-1/+ mothers. There is no difference in the force required to stall minus end travel of lipid droplets in embryos from Dhc64C8-1/+ mothers, although mean minus end travel distance is reduced.

Dhc64C8-1/Dhc64C6-10 animals survive to give fertile adults. Embryos laid by Dhc64C8-1/Dhc64C6-10 females develop seemingly normally through cellularisation and early gastrulation. However, lipid droplets fail to redistribute from the centre towards the periphery of these embryos during early cycle 14, in contrast to wild type. This results in embryos that are abnormally transparent from gastrulation onward.

Lethality acts during larval, pupal or pharate adult phase. Low levels (3-12%) of embryonic lethality are observed.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement
Reference

Dhc64C[+]/Dhc64C8-1 is an enhancer of visible | dominant phenotype of EgfrE1

Suppressor of
Statement
Reference

Dhc64C8-1 is a suppressor of visible | dominant phenotype of DCTN1-p1501

NOT Suppressor of
Statement
Reference

Dhc64C[+]/Dhc64C8-1 is a non-suppressor of visible | dominant phenotype of DCTN1-p1501, S[+]/S1

Dhc64C8-1/Dhc64C77 is a non-suppressor of visible | dominant phenotype of DCTN1-p1501, S[+]/S1

Phenotype Manifest In
Enhancer of
Statement
Reference

Dhc64C[+]/Dhc64C8-1 is an enhancer of eye phenotype of EgfrE1

Suppressor of
Statement
Reference

Dhc64C8-1 is a suppressor of eye phenotype of DCTN1-p1501

Dhc64C8-1/Dhc64C77 is a suppressor of eye phenotype of DCTN1-p1501

NOT Suppressor of
Statement
Reference

Dhc64C[+]/Dhc64C8-1 is a non-suppressor of eye phenotype of DCTN1-p1501, S[+]/S1

Dhc64C8-1/Dhc64C77 is a non-suppressor of eye phenotype of DCTN1-p1501, S[+]/S1

Additional Comments
Genetic Interactions
Statement
Reference

The S1 mutation overcomes the suppression of the Gl1 rough eye phenotype by Dhc64C8-1, so that triple heterozygotes have an enhanced rough eye phenotype compared to Gl1 single heterozygotes.

Gl1 Dhc64C77 Dhc64C8-1 flies have completely wild-type eyes.

The enhanced rough eye phenotype of Gl1/+ S1/+ flies is not suppressed by Dhc64C77/Dhc64C8-1.

Dhc64C8-1 dominantly enhances the eye phenotype of EgfrE1/+ flies, resulting in a reduced, narrower eye with fewer ommatidia.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Dhc64C8-1 is hemizygous lethal with Df(3L)10H but complements all Dhc64C mutations except Dhc64C1-1. Intragenic complementation relationships involve Dhc64C5-6, Dhc64C6-6, Dhc64C6-8, Dhc64C6-10, Dhc64C6-12 and Dhc64C8-1.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Name Synonyms
Secondary FlyBase IDs
  • FBal0104869
References (9)