FlyBase Allele Report: Dmel\kek1[RM2]

General Information

Symbol

Dmel\kek1^RM2

Species

D. melanogaster

Name

FlyBase ID

FBal0051472

Feature type

allele

Associated gene

Dmel\kek1

Associated Insertion(s)

Carried in Construct

Also Known As

RM2

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Musacchio and Perrimon, 1996)

Progenitor genotype

kek1^15A6

(Musacchio and Perrimon, 1996)

Cytology

Description

Imprecise excision of the P{lArB} insertion.

(Musacchio and Perrimon, 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Musacchio and Perrimon, 1996)

visible (with kek1^RA5)

(Alvarado et al., 2004)

Phenotype Manifest In

dorsal appendage

(Ghiglione et al., 1999)

dorsal appendage (with kek1^RA5)

(Zartman et al., 2009)

dorsal appendage | maternal effect (with kek1^RA5)

(Zartman et al., 2009)

egg

(Ghiglione et al., 1999)

wing vein | ectopic (with kek1^RA5)

(Alvarado et al., 2004)

Detailed Description

Statement

Reference

Eggs derived from kek1^RA5/kek1^RM2 females have thin and widely spaced dorsal appendages.

(Zartman et al., 2009)

kek1^RA5/kek1^RM2 animals have patches of ectopic vein in the wing (72.3% penetrance).

(Alvarado et al., 2004)

Eggs derived from kek1^RA5/kek1^RM2 females are mildly dorsalised; the spacing between the dorsal appendages is increased compared to wild-type. The eggs are slightly shorter and rounder than normal. This weak dorsalisation does not interfere with fertility.

(Ghiglione et al., 1999)

No major abnormalities are seen in the CNS of homozygous embryos, although partial defasciculation of Fas2 expressing axon bundles is occasionally seen.

(Speicher et al., 1998)

kek1^RA5/kek1^RM2 heterozygotes are viable, fertile, lay eggs with normal morphology and resulting adults have no obvious visible phenotype.

(Musacchio and Perrimon, 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

kek1^RA5/kek1^RM2 is a suppressor of visible phenotype of Egfr^t1/Egfr^f24

(Alvarado et al., 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

kek1^RA5/kek1^RM2 has egg phenotype, enhanceable by grk^+t9

(Ghiglione et al., 1999)

Suppressor of

Statement

Reference

kek1^RA5/kek1^RM2 is a suppressor of eye phenotype of Egfr^flb-2E07/Egfr^t1

(Alvarado et al., 2004)

kek1[+]/kek1^RM2 is a suppressor | partially of eye phenotype of Egfr^flb-2E07/Egfr^t1

(Alvarado et al., 2004)

kek1^RA5/kek1^RM2 is a suppressor of wing vein L4 phenotype of Egfr^t1/Egfr^f24

(Alvarado et al., 2004)

kek1^RA5/kek1^RM2 is a suppressor | partially of egg phenotype of Egfr^flb-2E07/Egfr^t1

(Ghiglione et al., 1999)

kek1^RA5/kek1^RM2 is a suppressor | partially of dorsal appendage phenotype of Egfr^flb-2E07/Egfr^t1

(Ghiglione et al., 1999)

Other

Statement

Reference

Nrt⁵, kek1^RM2 has axon | embryonic stage phenotype

(Speicher et al., 1998)

Nrt⁵, kek1^RA5/kek1^RM2 has axon | embryonic stage phenotype

(Speicher et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

Heterozygous kek1^RM2 or kek1^RA5 partially suppresses the rough eye phenotype seen Egfr^flb-2E07/Egfr^t1 animals. kek1^RM2/kek1^RA5 fully suppresses the phenotype.

(Alvarado et al., 2004)

The loss of wing vein L4 seen in Egfr^t1/Egfr^f24 animals is suppressed if they are also mutant for kek1 (kek1^RA5/kek1^RM2).

(Alvarado et al., 2004)

The dorsalised phenotype of eggs derived from kek1^RA5/kek1^RM2 females is enhanced by one copy of grk^+t9. The ventralised phenotype of eggs derived from Egfr^t1/Egfr^flb-2E07 females is partially suppressed by kek1^RA5/kek1^RM2.

(Ghiglione et al., 1999)

Extension of longitudinal axons through the intercommissural region is frequently abnormal in kek1^RM2; Nrt⁵ and kek1^RA5/kek1^RM2 ; Nrt⁵ embryos. In addition, Fas2 expressing axons show defasciculation, stalling and guidance defects, including crossing of the midline. The stalling and misguidance phenotypes can be rescued by Nrt^Scer\UAS.cSa expressed under the control of Scer\GAL4^Mz1277.

(Speicher et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

RM2

(Zartman et al., 2009, Ghiglione et al., 1999)

kek1^RM2

(Deignan et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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