Ectopic expression of NetBScer\UAS.T:Hsap\MYC throughout the somatic and visceral mesoderm, driven by Scer\GAL4twi.PB, causes salivary gland guidance defects. In 16% of these embryos, the distal tip of the salivary gland splits into two branches; one branch continues to migrate along the visceral mesoderm, while the other migrates towards lateral somatic muscles. Expression of NetBScer\UAS.T:Hsap\MYC throughout the CNS, driven by Scer\GAL4sca.PU, causes the distal tip of the salivary gland to become curved medially toward the CNS, instead of lying parallel to the CNS. However, expression of NetBScer\UAS.T:Hsap\MYC in the CNS with Scer\GAL4elav.PLu, does not cause misorientation of the salivary gland.
When expression is driven by Scer\GAL4how-24B SNa axons often stall at the edge of the CNS or fasciculate with the ISN.
Scer\GAL4sca-537.4 and Scer\GAL4elav.PLu induced expression in embryos causes commissures that are thinner or nearly absent and bundles of axons that project inappropriately laterally toward the edge of the CNS leaving gaps in the longitudinal tracts. Scer\GAL4how-24B induced expression causes excessive branching of the intersegmental nerve and these branches to stall short of their targets.