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General Information
Symbol
Dmel\NetBUAS.Tag:MYC
Species
D. melanogaster
Name
FlyBase ID
FBal0052146
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-NetB
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a full-length NetB cDNA, tagged at the C-terminal end with the Tag:MYC epitope.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Overexpression of NetBScer\UAS.T:Hsap\MYC, under the control of Scer\GAL4MH56, which has strong activity in the lamina neurons L3 throughout pupal development, does not interfere with R8 axon targeting.

Expression of NetBScer\UAS.T:Hsap\MYC under the control of Scer\GAL4MH56 in a Df(1)NetABΔ background significantly rescues the R8 axon-targeting defects. Only 8% of Rh6-expressing neurons stall at the medulla neuropil border or terminate in the distal M1/M2 layers, compared to 61% in mutant siblings lacking NetBScer\UAS.T:Hsap\MYC.

Ectopic expression of NetBScer\UAS.T:Hsap\MYC throughout the somatic and visceral mesoderm, driven by Scer\GAL4twi.PB, causes salivary gland guidance defects. In 16% of these embryos, the distal tip of the salivary gland splits into two branches; one branch continues to migrate along the visceral mesoderm, while the other migrates towards lateral somatic muscles. Expression of NetBScer\UAS.T:Hsap\MYC throughout the CNS, driven by Scer\GAL4sca.PU, causes the distal tip of the salivary gland to become curved medially toward the CNS, instead of lying parallel to the CNS. However, expression of NetBScer\UAS.T:Hsap\MYC in the CNS with Scer\GAL4elav.PLu, does not cause misorientation of the salivary gland.

When expression is driven by Scer\GAL4how-24B SNa axons often stall at the edge of the CNS or fasciculate with the ISN.

Scer\GAL4sca-537.4 and Scer\GAL4elav.PLu induced expression in embryos causes commissures that are thinner or nearly absent and bundles of axons that project inappropriately laterally toward the edge of the CNS leaving gaps in the longitudinal tracts. Scer\GAL4how-24B induced expression causes excessive branching of the intersegmental nerve and these branches to stall short of their targets.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The SNa pathfinding defect can be suppressed by injection of unc-5dsRNA.cKa RNA.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of NetBScer\UAS.T:Hsap\MYC under the control of Scer\GAL4sim.PS only weakly rescues the defects in interface glial cell migration seen in Df(1)NetABΔ embryos. A clear rescue of the stalling phenotype of the peripheral embryonic glia is seen in these animals.

Expression of NetBScer\UAS.T:Hsap\MYC under the control of Scer\GAL4sca-537.4 completely rescues the defects in interface glial cell migration seen in Df(1)NetABΔ embryos in 90% of embryos.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
NetBScer\UAS.T:Hsap\MYC
NetBUAS.T:Myc
NetBUAS.Tag:MYC
Name Synonyms
Secondary FlyBase IDs
    References (7)