FB2025_01 , released February 20, 2025
Allele: Dmel\vnUAS.cSa
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General Information
Symbol
Dmel\vnUAS.cSa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Schnepp
FlyBase ID
FBal0055941
Feature type
allele
Associated gene
Dmel\vn
Associated Insertion(s)
Carried in Construct
P{UAS-vn.S}
Also Known As
UAS-vn, UAS-vein
Key Links
Transgenic product class
cDNA
(Schnepp et al., 1996)
wild_type
(Schnepp et al., 1996)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Schnepp et al., 1996)
wild_type
(Schnepp et al., 1996)
Mutagen
in vitro construct
(Schnepp et al., 1996)
Progenitor genotype
Carried in construct
P{UAS-vn.S}
Cytology
Description

Expression of a vn cDNA is regulated by UASt sequences.

(Schnepp et al., 1996)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
vn
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of vnUAS.cSa under the control of either Scer\GAL4repo.PL or Scer\GAL4elav.PU does not affect the axon wrapping by wrapping glia in third instar larvae.

      (Matzat et al., 2015)

      Expression of vnScer\UAS.cSa under the control of Scer\GAL4en-e16E results in lethality at the pupal stage, with only 6% of animals surviving long enough to die as pharate adults with severe thoracic defects.

      (Butchar et al., 2012)

      Expression of vnScer\UAS.cSa in the adult visceral muscle, under the control of Scer\GAL4how-24B and Scer\GAL80ts.αTub84B, results in a mild increase in mitotic intestinal stem cells.

      (Jiang et al., 2011)

      Expression of vnScer\UAS.cSa under the control of Scer\GAL4Dot.PK has no effect on lamellocyte number in unstressed conditions.

      (Sinenko et al., 2011)

      Expression of vnScer\UAS.cSa in the visceral muscle under the control of Scer\GAL4how-24B and Scer\GAL80ts.αTub84B slightly stimulates intestinal stem cell (ISC) proliferation.

      (Buchon et al., 2010)

      Expression of vnScer\UAS.cSa driven by Scer\GAL4esg-NP7397 has little effect on adult midgut progenitor cell proliferation.

      (Jiang and Edgar, 2009)

      Border cell migration is normal when vnScer\UAS.cSa is expressed in anterior follicle cell clones under the control of Scer\GAL4cb41.

      Expression of two copies of vnScer\UAS.cSa in the border cell cluster, driven by Scer\GAL4slbo.2.6, causes a border cell misguidance phenotype.

      (McDonald et al., 2006)

      Expression of vnScer\UAS.cSa under the control of Scer\GAL4bi-md653 results in a mild haltere-to-wing transformation as the capitellum of the halteres produce one or two wing margin-type bristles.

      (Pallavi et al., 2006)

      Expression of vnScer\UAS.cSa under the control of Scer\GAL469B results in an extra vein phenotype in the wing. Expression of vnScer\UAS.cSa under the control of Scer\GAL471B results in a slight extra vein phenotype in the wing. Expression of vnScer\UAS.cSa under the control of Scer\GAL4bs-1348 results in an extra vein phenotype in the wing. Co-expression of vnΔEGF.Scer\UAS suppresses the extra wing vein phenotype caused by expression of vnScer\UAS.cSa under the control of Scer\GAL469B. Expression of vnScer\UAS.cSa under the control of Scer\GAL4T80 results in mild larval bloating. BrdU incorporation in the imaginal discs and brain is normal in these larvae. The P{UAS-vn.S}1.1 insertion shows a mild constitutive extra vein phenotype in the wing in the absence of an Scer\GAL4 driver caused by leaky expression of the transgene.

      (Donaldson et al., 2004)

      About a third of vnScer\UAS.cSa; Scer\GAL4αTub84B.PL somatic clones in the peripodial epithelium of the wing disc are transformed from squamous to columnar morphology. The transformation also affects some cells outside these clones.

      (Baena-Lopez et al., 2003)

      Expression of vnScer\UAS.cSa under the control of Scer\GAL4kni.L2 results in ectopic veins forming 2-3 cell diameters away from the L2 wing vein.

      (Lunde et al., 2003)

      vnScer\UAS.cSa; Scer\GAL4elav.PLu flies have a wild-type pattern of axons/fascicles in the embryonic central nervous system.

      (Hidalgo et al., 2001)

      Expression of vnScer\UAS.cSa under the control of Scer\GAL4ey.PB does not result in homeotic transformation of the eye to antenna.

      (Kumar and Moses, 2001)

      Expression of vnScer\UAS.cSa under the control of Scer\GAL4dpp.blk1 does not result in ectopic furrow formation in the eye disc.

      (Kumar and Moses, 2001)

      Expression of vnScer\UAS.cSa under the control of Scer\GAL4sim.P3.7 in the midline glia (MG) beyond stage 12 does not result in an increase in the number of surviving MG cells.

      (Lanoue et al., 2000)

      vnScer\UAS.cSa/Scer\GAL471B males are poorly viable and have wings with ectopic vein tissue spanning the regions between L3 and the anterior margin and L4 to L5. vnScer\UAS.cSa driven by Scer\GAL4ptc-559.1 has no effect on the spacing of wing veins L3 and L4. However small ectopic veins can be seen.

      (Wessells et al., 1999)

      Scer\GAL469B-mediated expression causes a mild or moderate extra-vein phenotype, but has no effect on the eye phenotype. Wing is also smaller than wild type as vein cells that replace inter vein cells are more compact. Scer\GAL4bs-1348-mediated expression causes a mild or moderate extra-vein phenotype. Scer\GAL4GMR.PF-mediated expression has no effect on the adult eye phenotype.

      (Schnepp et al., 1998)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Statement
      Reference

      Scer\GAL4bi-md653, vnUAS.cSa has visible | homeotic phenotype, enhanceable by EgfrUAS.cBa, Scer\GAL4bi-md653

      (Pallavi et al., 2006)

      Scer\GAL4bi-md653, vnUAS.cSa has visible | homeotic phenotype, enhanceable by Ubx1/Ubx[+]

      (Pallavi et al., 2006)

      vnUAS.cSa has visible phenotype, enhanceable by Prosβ61

      (Donaldson et al., 2004)

      vnUAS.cSa has visible phenotype, enhanceable by Prosβ21

      (Donaldson et al., 2004)
      Suppressed by
      Enhancer of
      NOT Enhancer of
      Suppressor of
      NOT Suppressor of
      Other
      Phenotype Manifest In
      Enhanced by
      Suppressed by
      Statement
      Reference

      Scer\GAL471B, vnUAS.cSa has wing vein | ectopic phenotype, suppressible by Scer\GAL471B/aosUAS.cHa

      (Wessells et al., 1999)
      Enhancer of
      NOT Enhancer of
      Suppressor of
      NOT Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of Sulf1Scer\UAS.cBa suppresses the lethality caused by expression of vnScer\UAS.cSa under the control of Scer\GAL4en-e16E such that some adults survive to adulthood. The survivors have extra wing veins and blisters in the posterior region of the wing.

      (Butchar et al., 2012)

      Expression of vnScer\UAS.cSa results in an enhancement of the haltere-to-wing transformation of Ubxlac1-GAL4/+ mutants as the capitellum of the halteres produce 30-35 wing margin-type bristles in vnScer\UAS.cSa; Ubxlac1-GAL4/+ flies while there are only 4-7 bristles in Ubxlac1-GAL4/+ flies. The halteres of Ubxlac1-GAL4/+ flies expressing vnScer\UAS.cSa also show increased pigmentation compared to Ubxlac1-GAL4/+ or wild-type flies. Coexpression of vnScer\UAS.cSa and EgfrScer\UAS.cBa in a Ubxlac1-GAL4/+ background results in a further enhancement of the phenotype.

      Coexpression of vnScer\UAS.cSa and EgfrScer\UAS.cBa under the control of Scer\GAL4bi-md653 results in an enhancement of the haltere-to-wing transformation see when either transgene is expressed alone. This is an enhancement rather than an additive effect as the capitellum of the haltere produces between 10 and 22 bristles in the double transgene-expressing flies vs 5 at the most in the single transgene flies.

      There is a marginal enhancement in the haltere-to-wing phenotype in Scer\GAL4bi-md653>vnScer\UAS.cSa; Ubx1/+ flies compared to either Ubx1/+ or Scer\GAL4bi-md653>vnScer\UAS.cSa flies.

      (Pallavi et al., 2006)

      The constitutive extra vein phenotype in the wing caused by the P{UAS-vn.S}1.1 insertion in the absence of an Scer\GAL4 driver is enhanced in combination with Pros261 or Prosβ21 and following a shift to the restrictive temperature.

      (Donaldson et al., 2004)

      Expression of vnScer\UAS.cSa under the control of Scer\GAL4dpp.blk1 does not rescue wing vein L4 formation in kncol-1 animals rescued with kn+m5, while the larger L3-type vein seen in these animals is widened.

      (Crozatier et al., 2002)

      Co-expression of EgfrDN.Scer\UAS.cBa, vnScer\UAS.cSa and rhoScer\UAS.cBa driven by Scer\GAL4Bx-MS1096, does not suppress the EgfrDN.Scer\UAS.cBa induced loss of vein phenotype nor restore the rhoScer\UAS.cBa induced ectopic vein phenotype.

      (Guichard et al., 1999)

      The addition of argosScer\UAS.cHa produces a phenotype intermediate of those caused by argosScer\UAS.cHa or vnScer\UAS.cSa alone (driven by Scer\GAL471B); the vein loss phenotype of argosScer\UAS.cHa is partially rescued, the extra vein phenotype of vnScer\UAS.cSa is rescued. The addition of vnScer\UAS.cSa suppresses the loss of wing vein phenotype seen in ptcScer\UAS.cJa/Scer\GAL471B flies, restoring the anterior cross vein.

      (Wessells et al., 1999)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4NP0001/vnUAS.cSa rescues vn10567

      (Jiang and Edgar, 2009)

      Scer\GAL4NP7397/vnUAS.cSa rescues vn10567

      (Jiang and Edgar, 2009)

      Scer\GAL4how-24B/vnUAS.cSa rescues vn10567

      (Jiang and Edgar, 2009)

      Scer\GAL4αTub84B.PZ/vnUAS.cSa rescues vnL6/vnddd-4

      (Zecca and Struhl, 2002)
      Partially rescues

      vnUAS.cSa/Scer\GAL4vn-GAL4 partially rescues vnL6/vn1

      (Austin et al., 2014)

      vnUAS.cSa/Scer\GAL4elav.PLu partially rescues Df(3L)γ3/vnγ4

      (Hidalgo et al., 2001)

      vnUAS.cSa/Scer\GAL4T80 partially rescues Df(3L)γ3/vnL6

      (Schnepp et al., 1996)
      Comments

      Expression of vnScer\UAS.cSa under the control of Scer\GAL4vn.GAL4 partially rescues the lethality seen in vnL6/vnGAL4 mutant flies. In optimal conditions approximately 30% of flies reach adulthood and have grossly normal patterning of the wing. Some extra vein material is observed. The wing disc phenotype is partially rescues and no leg defects are observed. The extent of the rescue depends on the expression level.

      (Austin et al., 2014)

      Expression of vnScer\UAS.cSa in larval enterocytes driven by Scer\GAL4Myo31DF-NP0001 not only rescues the adult midgut progenitor cell phenotype of vn10567 mutants, but it also causes ectopic AMP cell proliferation.

      Expression of vnScer\UAS.cSa in adult midgut progenitor cells (AMPs) rescues the AMP cell proliferation phenotype of vn10567 mutants.

      Expression of vnScer\UAS.cSa in visceral muscle driven by Scer\GAL4how-24B rescues the adult midgut progenitor cell phenotype of vn10567 mutants.

      (Jiang and Edgar, 2009)

      vnL6/vnddd-4 wing discs containing multiple clones expressing vnScer\UAS.cSa under the control of Scer\GAL4αTub84B.PZ appear similar in size to wild-type discs. Single clones expressing vnScer\UAS.cSa under the control of Scer\GAL4αTub84B.PZ can confer at least partial and sometimes extensive rescue of vnL6/vnddd-4 wing discs. Rescue is only seen when the clones are located in or near the prospective dorsal region of the disc.

      (Zecca and Struhl, 2002)

      Interface glial cell survival is rescued in vnγ4/Df(3L)γ3 embryos by vnScer\UAS.cSa; Scer\GAL4elav.PLu, and partially rescued by vnScer\UAS.cSa; Scer\GAL415J2.

      (Hidalgo et al., 2001)

      When expressed via Scer\GAL4T80, vnScer\UAS.cSa partially rescues the pattern defects in vnL6/Df(3L)γ3 larvae and the proliferation defect in vnL6/Df(3L)γ3 mutant wing discs.

      (Schnepp et al., 1996)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      vnScer\UAS.cSa
      vnUAS.cSa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Schnepp
      (Schnepp et al., 1996)
      Secondary FlyBase IDs
        References (32)