Expression of numbScer\UAS.cWa in the wing disc under the control of Scer\GAL4ap-md544 inhibits the production of hairs during asymmetric division of the sensory organ precursors, which results in bald nota in all progeny.
Expression of numbScer\UAS.cWa in peripheral glial cells under the control of Scer\GAL4Mz97 leads to an impairment of glial cell migration. The ventral peripheral glial cells and the ventral intersegmental peripheral glial cells stall at the exit zone, while the medial intersegmental peripheral glial cells migrate further laterally. Ventral peripheral glia in which numbScer\UAS.cWa is not expressed due to the pattern of the Scer\GAL4Mz97 driver migrate dorsally.
Compared to control clones in third instar larvae, clones with expression of numbScer\UAS.cWa have a cell cycle delay: there are a reduced number of neuroepithelial stem cells that have not differentiated into neurons on the lateral surface of the brain, and post-embryonic neuroblasts contain smaller numbers of neurons and ganglion mother cells; Hsap\NUMBScer\UAS.PTBS-PRRS clones containing only neurons on the medial surface near the central brain are found at a higher frequency than control clones.
When numbScer\UAS.cWa is driven by Scer\GAL4sca-P309, a strong reduction in external structures on the adult antenna is seen, with a concomitant increase in glial cell numbers, with a concomitant increase in glial cell numbers. The diameter of fascicles in antennal segment 3 are also increased.
When numbScer\UAS.cWa is driven by Scer\GAL4hs.PB from 3.5 to 7.5 hours after egg laying, Malpighian tubules have two tip cells, instead of one as seen in wild-type. However, there is no change in the final number of tubule cells. The distinction between the number of cells in the anterior and posterior tubules is also unaffected.
The number of eve-expressing pericardial cells is reduced and the number of DA1 muscles is increased in embryos expressing numbScer\UAS.cWa under the control of Scer\GAL4twi.PG.
Embryos expressing numbScer\UAS.cWa under the control of Scer\GAL4twi.PB show duplications and transformations of muscles, and adult muscle precursors are lost. Muscle VA2 is duplicated at the expense of VA1, DO5 is transformed to LL1 and LT3 is transformed to LT4. The ventral adult muscle precursors are missing and muscle VA3 is duplicated. Lateral and dorsal adult muscle precursors are also missing, and the segment border muscle and dorsal muscles (including DA1) are duplicated. Embryos lack pericardial cells. Despite the muscle aberrations, some embryos develop to form adult flies. These flies are flightless, do not jump and lack ventral abdominal muscles that would normally be generated from the ventral adult muscle precursors.
Scer\GAL4sca-109-68-mediated expression causes twinned hairs and balding. Phenotype is caused by transformation of both the IIa and IIb cell and the sheath to a neuron.
When expression is driven by Scer\GAL4ptc-559.1, at 29oC during the second larval instar stage, adult flies show a loss of distal wing margin at the anterior/posterior boundary. When expressed in the sensory organ precursor cells of the adult external sense organs, using Scer\GAL4sca-109-68, a twinned hair phenotype is produced.