FlyBase Allele Report: Dmel\bnl[00857]

bnl⁰⁰⁸⁵⁷ mutant eye clones lose their ability to constrict their apical surfaces at the morphogenetic furrow. They also lose normal contacts between cells and form aberrant-shaped clusters.

bnl⁰⁰⁸⁵⁷ mutant eye clones produce defects in retinal basal glial (RBG) cell migration from second instar larval stage. The RBG cells migrate ectopically to anterior regions of the eye beyond the morphogenetic furrow, a phenotype that is not seen in wild type flies. RBG proliferation and survival are not affected. bnl⁰⁰⁸⁵⁷ clones generated specifically in the glia do not show RBG migration defects.

(Mukherjee et al., 2012)

40% of tracheal ganglionic branches fail to reach the central nervous system in heterozygous embryos.

(Hemphala et al., 2003)

Somatic clones of bnl⁰⁰⁸⁵⁷ in the third instar wing disc columnar epithelium cause a decrease in the numbers and migration of air sac tracheoblasts.

(Sato and Kornberg, 2002)

The filopodia-like cell extensions that are seen in tracheal cells at stage 12 are missing in mutant embryos. The mutant embryos lack an interconnected dorsal trunk and instead show a segmental array of isolated metameric tracheal units with a lollipop-like structure in place of the dorsal trunk.

(Wolf et al., 2002)

Stage 13 homozygous embryos show little tracheal branch outgrowth.

(Shim et al., 2001)

Heterozygous and hemizygous embryos show sporadic defects in primary tracheal branch outgrowth. This is manifest most significantly by the ganglionic tracheal branches, 9% of which fail to reach the central nervous system.

(Imam et al., 1999)

The number of terminal tracheal branches (GB, LG and LH) are reduced by 30%-50% in heterozygous bnl⁰⁰⁸⁵⁷ larvae.

(Jarecki et al., 1999)

Almost every tracheal metamere appears as an unbranched, elongate sac of tracheal cells. Tracheal cells invaginate and form tracheal sacs but the branches fail to grow out. A few primary branches form that are rudimentary. Scer\GAL4^C49 mediated expression of bnl^Scer\UAS.cSa allows branch formation and growth. Formation of muscles and the peripheral nervous system are unaffected. There are specific defects in the nervous system.

(Sutherland et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

bnl⁰⁰⁸⁵⁷ has abnormal cell migration phenotype, suppressible by ptc[+]/ptc^D130

(Butí et al., 2014)

Enhancer of

Statement

Reference

bnl[+]/bnl⁰⁰⁸⁵⁷ is an enhancer of visible | adult stage phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Feuillette et al., 2020)

Other

Statement

Reference

bnl⁰⁰⁸⁵⁷, sr¹⁵⁵ has abnormal cell migration phenotype

(Butí et al., 2014)

Phenotype Manifest In

Enhanced by

Statement

Reference

bnl⁰⁰⁸⁵⁷ has embryonic/larval ganglionic tracheal branch phenotype, enhanceable by stumps^09904b

(Imam et al., 1999)

bnl⁰⁰⁸⁵⁷ has embryonic/larval dorsal tracheal branch phenotype, enhanceable by stumps^09904b

(Imam et al., 1999)

Suppressed by

Statement

Reference

bnl⁰⁰⁸⁵⁷ has embryonic/larval ganglionic tracheal branch phenotype, suppressible by ptc[+]/ptc^D130

(Butí et al., 2014)

bnl⁰⁰⁸⁵⁷ has larval tracheal system phenotype, suppressible by ptc[+]/ptc^D130

(Butí et al., 2014)

bnl⁰⁰⁸⁵⁷ has ganglionic tracheal branch primordium phenotype, suppressible by grh^s2140/grh^s2140

(Hemphala et al., 2003)

bnl⁰⁰⁸⁵⁷ has embryonic/larval tracheal dorsal trunk phenotype, suppressible by Scer\GAL4^btl.PS/Cdc42^V12.UAS

(Wolf et al., 2002)

bnl⁰⁰⁸⁵⁷ has larval tracheal system phenotype, suppressible by btl::tor^{t4021b.hs.sev}

(Sutherland et al., 1996)

Enhancer of

Statement

Reference

bnl[+]/bnl⁰⁰⁸⁵⁷ is an enhancer of eye phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Feuillette et al., 2020)

bnl[+]/bnl⁰⁰⁸⁵⁷ is an enhancer of dorsal group tracheal branch precursor phenotype of Rac1^J11, Rac2^Δ/Rac2[+]

(Chihara et al., 2003)

bnl[+]/bnl⁰⁰⁸⁵⁷ is an enhancer of tracheal branch primordium phenotype of Rac1^J11, Rac2^Δ/Rac2[+]

(Chihara et al., 2003)

bnl[+]/bnl⁰⁰⁸⁵⁷ is an enhancer of tracheal dorsal trunk primordium phenotype of Rac1^J11, Rac2^Δ/Rac2[+]

(Chihara et al., 2003)

Suppressor of

Statement

Reference

bnl[+]/bnl⁰⁰⁸⁵⁷ is a suppressor of tracheole phenotype of ago^Δ3-7/ago¹

(Mortimer and Moberg, 2013)

bnl[+]/bnl⁰⁰⁸⁵⁷ is a suppressor of larval lateral H tracheal branch phenotype of ago^Δ3-7/ago¹

(Mortimer and Moberg, 2013)

bnl[+]/bnl⁰⁰⁸⁵⁷ is a suppressor of tracheole | cell non-autonomous phenotype of Scer\GAL4^tey-5053A, ago^ΔF.UAS

(Mortimer and Moberg, 2013)

bnl[+]/bnl⁰⁰⁸⁵⁷ is a suppressor of embryonic/larval ganglionic tracheal branch phenotype of seq^Z1241

(Araújo and Casanova, 2011)

bnl[+]/bnl⁰⁰⁸⁵⁷ is a suppressor of embryonic/larval tracheal section phenotype of Scer\GAL4^btl.PS, Vhl^RNAi.UAS.cMa

(Mortimer and Moberg, 2009)

Other

Statement

Reference

bnl⁰⁰⁸⁵⁷, sr¹⁵⁵ has larval tracheal system phenotype

(Butí et al., 2014)

bnl⁰⁰⁸⁵⁷, sr¹⁵⁵ has embryonic/larval ganglionic tracheal branch phenotype

(Butí et al., 2014)

Pak⁶, bnl[+]/bnl⁰⁰⁸⁵⁷ has tracheal branch primordium phenotype

(Chihara et al., 2003)

Pak⁶, bnl[+]/bnl⁰⁰⁸⁵⁷ has dorsal group tracheal branch precursor phenotype

(Chihara et al., 2003)

Pak[+]/Pak⁶, bnl⁰⁰⁸⁵⁷ has dorsal group tracheal branch precursor phenotype

(Chihara et al., 2003)

Pak[+]/Pak⁶, bnl⁰⁰⁸⁵⁷ has tracheal dorsal trunk primordium phenotype

(Chihara et al., 2003)

Pak⁶, bnl[+]/bnl⁰⁰⁸⁵⁷ has tracheal dorsal trunk primordium phenotype

(Chihara et al., 2003)

Pak⁶, bnl⁰⁰⁸⁵⁷ has tracheal branch primordium phenotype

(Chihara et al., 2003)

Pak⁶, bnl⁰⁰⁸⁵⁷ has dorsal group tracheal branch precursor phenotype

(Chihara et al., 2003)

Pak⁶, bnl⁰⁰⁸⁵⁷ has tracheal dorsal trunk primordium phenotype

(Chihara et al., 2003)

Pak[+]/Pak⁶, bnl⁰⁰⁸⁵⁷ has tracheal branch primordium phenotype

(Chihara et al., 2003)

bnl⁰⁰⁸⁵⁷, stumps^09904b has embryonic/larval tracheal dorsal trunk phenotype

(Imam et al., 1999)

bnl⁰⁰⁸⁵⁷, btl^LG18 has larval tracheal system phenotype

(Sutherland et al., 1996)

bnl⁰⁰⁸⁵⁷, btl^LG19 has larval tracheal system phenotype

(Sutherland et al., 1996)

Additional Comments

Genetic Interactions

Statement

Reference

A ptc^D130 heterozygous mutant background suppresses the tracheal ganglionic branch migration defects seen in bnl⁰⁰⁸⁵⁷ mutants.

sr¹⁵⁵/bnl⁰⁰⁸⁵⁷ double mutants exhibit impaired ganglionic branch migration.

(Butí et al., 2014)

The increase in the number of tracheal branches that terminate on ventrolateral body wall muscle 12 caused by expression of ago^ΔF.Scer\UAS under the control of Scer\GAL4^tey-5053A is suppressed if the flies are also heterozygous for bnl⁰⁰⁸⁵⁷.

bnl⁰⁰⁸⁵⁷ dominantly suppresses the increase in number of terminal branches per lateral LH cell seen in ago¹/ago^Δ3-7 larvae.

(Mortimer and Moberg, 2013)

bnl⁰⁰⁸⁵⁷/+ leads to a significant reduction in the penetrance of Scer\GAL4^btl.PS Vhl^{dsRNA.Scer\UAS} tracheal phenotypes.

(Mortimer and Moberg, 2009)

Pak⁶; bnl⁰⁰⁸⁵⁷ double heterozygotes have a range of tracheal phenotypes not seen in single heterozygotes for either of these alleles: The mildest phenotype is a misrouting of the dorsal branches toward the anteroposterior direction; more severely effected embryos also exhibit truncations of the dorsal trunk.

(Chihara et al., 2003)

The bnl⁰⁰⁸⁵⁷/+ tracheal branch outgrowth defects are partially suppressed by grh^s2140/grh^s2140; 78% of the tracheal ganglionic branches reach the central nervous system in the double mutant embryos.

(Hemphala et al., 2003)

Expression of Cdc42^V12.Scer\UAS under the control of Scer\GAL4^btl.PS in a bnl⁰⁰⁸⁵⁷ background show occasional (4.4%) fusions of the adjacent lollipop-like dorsal trunk structures (bnl⁰⁰⁸⁵⁷ single mutants show no fusion of the adjacent metameric tracheal units).

(Wolf et al., 2002)

stumps^09904b, bnl⁰⁰⁸⁵⁷ double heterozygotes show a dramatic increase in tracheal outgrowth defects, with nearly four times as many stalled ganglionic branches as the bnl⁰⁰⁸⁵⁷ heterozygote alone. The double heterozygotes also displayed increased dorsal branch outgrowth defects compared to bnl⁰⁰⁸⁵⁷ heterozygotes as well as rare dorsal trunk outgrowth defects never seen in bnl⁰⁰⁸⁵⁷ heterozygotes.

(Imam et al., 1999)

btl^LG18 bnl⁰⁰⁸⁵⁷ double mutants exhibit a tracheal phenotype similar to either mutant alone. btl^LG19/btl⁺ bnl⁰⁰⁸⁵⁷/bnl⁺ double heterozygote exhibits enhanced bnl⁰⁰⁸⁵⁷ phenotype, defects in tracheal outgrowth. A constitutively activated form of the btl receptor (btl::tor^{t4021b.hs.sev}) can partially ameliorate the effect of absence of bnl; modest restoration of branching in bnl⁰⁰⁸⁵⁷ embryos.

(Sutherland et al., 1996)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

bnl⁷⁰⁸

(Myat et al., 2005)

bnl⁰⁶⁹¹⁶

(BDGP Project Members, 1994-1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

6384

ry⁵⁰⁶ P{PZ}bnl⁰⁰⁸⁵⁷/TM3, Sb¹

Notes on Origin

Discoverer

Comments

Complements: l(3)01344⁰¹³⁴⁴. Complements: l(3)05050⁰⁵⁰⁵⁰. Complements: sr^06915b. Complements: sr^06948b.

(BDGP Project Members, 1994-1999)

P{PZ} insertion carries an internal deletion than removes most of the Tn\kanR and Ecol\ori region. Δ2-3 mediated transposition yields a homozygous viable revertant that restores tracheal branching and other revertants with intermediate tracheal phenotype. This confirms the P{PZ} element causes the bnl phenotype.

(Sutherland et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

I(3)00857

(Spradling et al., 1999)

bnl⁰⁰⁸⁵⁷

(Feuillette et al., 2020, Mukherjee et al., 2012, Christensen et al., 2009.5.6, Christensen et al., 2009.5.6, Christensen et al., 2008.9.29)

bnl¹

(Inoue and Hayashi, 2007)

bnl^PI

(Park et al., 2003)

bnl^p1

(Hemphala et al., 2003)

l(3)0857

(Sutherland et al., 1996)

Name Synonyms

Secondary FlyBase IDs

References (39)

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