A transheterozygous combination of eveΔRP2A/+, unc-58/+ reduces beat-Ia levels to 50% and significantly increases the defects seen in intersegmental nerves, with the number of axons that exhibit stalling increasing from 3% to 7.5% in eveΔRP2A/+, unc-58/+ transheterozygotes and eveΔRP2A/+, unc-58/+, beat-Ia3/+ triple heterozygotes, respectively.
CNS exit from EW neurons misexpressing eveScer\UAS.cBa is partially suppressed in an unc-58 beat-IaC163/beat-Ia3 mutant background while midline crossing is partially restored (in 15% of hemisegments).
The neuromuscular junction innervation defects seen in the dorsal muscles of beat-Ia3/beat-IaC163 third instar larvae are not enhanced by expression of sideScer\UAS.cSa under the control of either Scer\GAL4how-24B or Scer\GAL4Mef2.PR.
beat-Ia/+, SoxNGA1192/+ double heterozygous embryos commissures are partially fused and the longitudinal connectives are thinner or missing. This phenotype is not seen in either beat-Ia/+ or SoxNGA1192/+ embryos.