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General Information
Symbol
Dmel\Nf1hs.PT
Species
D. melanogaster
Name
heat shock construct of The
FlyBase ID
FBal0061706
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

An Hsp70 promoter drives expression of Nf1. The construct includes the shorter Nf1 C-terminus predicted by exon 18b and lacks a 30 amino acid segment predicted by alternatively spliced exon 14.

Hybrid cDNA genomic Nf1 minigene (contains 3 introns) is expressed from an Hsp70 promoter.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Nf1hs.PT greatly increases the resistance to paraquat-induced oxidative stress, compared to wild-type.

Expression of Nf1hs.PT in a wild-type background increases aconitase activity by more than 90%.

Upregulation of Nf1hs.PT expression by maintaining these flies at 25[o]C throughout life results in an increase in mean life span of 49% for male flies and 68% for females and an increase in maximum life span of 38% for males and 52% for females. By contrast, when Nf1hs.PT flies are maintained at 25[o]C through embryogenesis and 4 days in adulthood but then switched to 18[o]C for the remainder of their adult life, results in the elimination of the extension of maximum life span and a reduction in the extension of mean life span. The mortality rates of these flies do not change compared to wild-type.

Both fertility and fecundity are higher in Nf1hs.PT-expressing flies compared to wild-type. This is not because the Nf1hs.PT flies are bigger, as they have similar body lengths to controls, although Nf1hs.PT females are slightly heavier.

Testing the stress resistance of Nf1hs.PT-overexpressing flies by assessing up-climbing ability reveals that these flies are highly resistant to heat stress and paraquat-induced oxidative stress, with Nf1hs.PT females showing a 56% increase in survival time and the males a 51% increase in survival time during paraquat exposure, relative to controls. However Nf1hs.PT flies are not more resistant to desiccation.

Mitochondria NADH-linked (pyruvate+malate) respiration rates from Nf1hs.PT overexpressing flies are 25%-38% higher than those of controls, in the presence of ADP, but not in the absence of ADP. Consequently, the respiratory control ratio is significantly higher for these mitochondria than for controls. Nf1hs.PT-overexpressing mitochondria have a 54% higher ATP production rate when metabolising NADH-linked substrates, compared to controls. In contrast, the respiration rates recorded when using the FADH[[2]]-linked complex II substrate succinate are essentially the same as in controls, with or without ADP.

Complex I activity is 28% higher in Nf1hs.PT-expressing flies compared to controls.

Mitochondrial H[[2]]O[[2]] production is reduced by 58-59% in Nf1hs.PT mutants compared to control flies. This reduced mitochondrial ROS is also associated with increased mitochondrial aconitase activity throughout life.

Expression of Nf1hs.PT in a wild-type background using heat shock has no effect on learning scores.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescues

Nf1hs.PT partially rescues Nf1P2

Comments

Nf1hs.PT rescues the neuromuscular junction overgrowth phenotype seen in Nf1E2/Nf1E2 larvae. The reduced body size of Nf1E2/Nf1E2 pupae is also rescued.

The presence of Nf1hs.PT rescues the short life span seen in Nf1P2 mutants.

The paraquat sensitivity of Nf1P2 mutant flies is eliminated in Nf1hs.PT/+; Nf1P2 mutants.

Mitochondrial aconitase activity is restored by expression of Nf1hs.PT in a Nf1P2 mutant background.

Elevated superoxide levels in Nf1P2 mutant flies are reduced to wild-type levels by the introduction of Nf1hs.PT.

The pupal size defect seen in Nf1E2 animals is rescued by expression of Nf1hs.PT using heat shock.

Addition of Nf1hs.PT to Nf1P2 animals fully rescues the perineurial glial thickness phenotype.

Rescues the learning defects of Nf1P2 flies when expressed using heat shock during adult stages. Expression of Nf1hs.PT using a single heat shock during larval stages does not rescue the smaller body size of Nf1P2 flies.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Nf1hs.PT
Name Synonyms
heat shock construct of The
Secondary FlyBase IDs
    References (8)