Open Close
General Information
Symbol
Dmel\Mer3
Species
D. melanogaster
Name
FlyBase ID
FBal0061732
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

G19692427H

Amino acid change:

M177I | Mer-PA; M177I | Mer-PB

Reported amino acid change:

M177I

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. Nucleotide substitution not specified (could be G to A, G to T, or G to C)

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: M177I.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The seminal vesicles of mutant males are smaller than normal and are shrivelled. Very few sperm are found in the seminal vesicles and those that are found are immotile. The testes have fewer sperm heads in each bundle than normal and the bundles are disorganised.

No abnormalities are found during mitosis of spermatogonia or spermatocyte growth in the mutant testes.

Three types of abnormality are occasionally found during meiosis of spermatocytes. In approximately 5% of cases, spermatids containing two nuclei of equal size and two Nebenkerns are seen. Rarely, tripolar or four-polar spindles are seen in spermatocytes undergoing the second meiotic division.

Spermatid nuclei in elongating cysts are grouped into two locations in some cysts and appear more scattered than normal in others.

Some mutant spermatids at the late stage of spermatid elongation contain two paracrystalline bodies within the major mitochondrial derivative compared to one in control cysts. Some mutant spermatids have two axonemes present compared to only one in control cysts. Mutant cysts are loosely arranged compared to control cysts, which display cell-cell contact. Some cytoplasmic shedding is present mutant cysts.

The sperm nuclei in mutant spermatid cysts undergoing individualization have variable morphology and appear scattered. The actin cones are dispersed.

At the individualisation stage, mutant spermatids show variable phenotypes, including two paracrystalline body-filled mitochondrial derivatives with abnormal shape, or three paracrystalline body-filled mitochondrial derivatives together with one axoneme, or spermatids having one or two paracrystalline-filled mitochondrial derivatives but no axoneme compared to control spermatids.

Mutant cysts show gross disorganization in the arrangement of the individualized spermatids. Some spermatids appear fused together.

Hemizygous males have broadened wings and show a low penetrance of disruptions of the posterior crossvein. The eyes are slightly rough and smaller than normal, and 10% have ectopic growths and vibrissae almost exclusively in the anterior ventral portion of the eye. Minor perturbation of ommatidial organisation is seen. Approximately 50% of the expected number of Mer3/Y males eclose.

Mosaic egg chambers in which all the follicle cells are homozygous, or in which a large homozygous clone covers all the posterior follicle cells show a strong Mer mutant phenotype indistinguishable from non-mosaic homozygous mutants. The oocyte nucleus fails to migrate. Mosaic egg chambers with homozygous clones in the anterior follicle cells or main body follicle cells do not have a Mer mutant phenotype. In mosaic egg chambers in which only a few posterior follicle cells are homozygous, the heterozygous cells rescue the Mer mutant phenotype in all the neighbouring follicle cells and also rescue the oocyte nuclear migration phenotype, indicating that Mer acts non-autonomously.

The ventral portion of the eye disc is significantly enlarged and the morphogenetic furrow appears to neither initiate or progress through the ectopic tissue in hemizygous animals. In less than 5% of cases, an ectopic morphogenetic furrow develops in the ventral portion of the disc with consequent neuronal differentiation. Hemizygous adults have a weakly roughened, reduced eye with expansion of ventral and posterior head cuticle and development of ectopic vibrissae (the bristles found ventral and anterior to the eye). There are only minor perturbations in interommatidial organisation and no obvious disruptions in ommatidial polarity. Hemizygous adults have broadened wing blades and may show disruption of the posterior crossvein. The increased wing size appears to be due to an increase in cell number rather than an increase in cell size and in fact cell size is slightly decreased compared to wild type.

Homozygotes have broadened wings, and show a low and variably penetrant expression of weakly roughened eyes and development of abnormal head cuticle structures. Homozygous clones in the eye have a normal overall morphology, although occasionally a very weak roughened phenotype is seen. The clones are significantly larger than their wild-type sister clones.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

Mer3 has visible phenotype, enhanceable by Df(2L)C144/+

Mer3 has visible phenotype, enhanceable by +/In(2R)bwVDe2LCyR

Mer3 has visible phenotype, enhanceable by bs03267/bs[+]

Mer3 has visible phenotype, enhanceable by bs[+]/bs242

Mer3 has visible phenotype, enhanceable by bs[+]/bs253

Mer3 has visible phenotype, enhanceable by bs364/bs[+]

Mer3 has visible | recessive phenotype, enhanceable by dpphr56/dpp[+]

Mer3 has visible | recessive phenotype, enhanceable by exe1

Mer3 has visible | recessive phenotype, enhanceable by ex697

NOT Enhanced by
Statement
Reference

Mer3 has partially lethal | recessive | male phenotype, non-enhanceable by bs242

Mer3 has partially lethal | recessive | male phenotype, non-enhanceable by bs253

Mer3 has partially lethal | recessive | male phenotype, non-enhanceable by net107

Mer3 has partially lethal | recessive | male phenotype, non-enhanceable by net383

Suppressed by
Statement
Reference

Mer3 has visible phenotype, suppressible by bs03267/bs[+]

Mer3 has visible phenotype, suppressible by bs[+]/bs242

Mer3 has visible phenotype, suppressible by bs[+]/bs253

Mer3 has visible phenotype, suppressible by bs364/bs[+]

Mer3 has visible phenotype, suppressible by net107/net[+]

Mer3 has visible phenotype, suppressible by net[+]/net383

Other
Statement
Reference
Phenotype Manifest In
Enhanced by
Statement
Reference

Mer3 has posterior crossvein phenotype, enhanceable by bs242

Mer3 has eye phenotype, enhanceable by Df(2L)C144/+

Mer3 has ommatidium phenotype, enhanceable by Df(2L)C144/+

Mer3 has vibrissae phenotype, enhanceable by Df(2L)C144/+

Mer3 has eye phenotype, enhanceable by +/In(2R)bwVDe2LCyR

Mer3 has ommatidium phenotype, enhanceable by +/In(2R)bwVDe2LCyR

Mer3 has vibrissae phenotype, enhanceable by +/In(2R)bwVDe2LCyR

Mer3 has eye phenotype, enhanceable by dpphr56/dpp[+]

Mer3 has adult cuticle & head phenotype, enhanceable by exe1

Mer3 has ommatidium phenotype, enhanceable by dpphr56/dpp[+]

Mer3 has interommatidial bristle phenotype, enhanceable by dpphr56/dpp[+]

Mer3 has adult head & cuticle phenotype, enhanceable by dpphr56/dpp[+]

Mer3 has eye phenotype, enhanceable by ex697

Mer3 has vibrissae | ectopic phenotype, enhanceable by ex697

Mer3 has adult cuticle & head phenotype, enhanceable by ex697

NOT Enhanced by
Statement
Reference

Mer3 has head phenotype, non-enhanceable by bs242

Mer3 has leg phenotype, non-enhanceable by bs242

Mer3 has head phenotype, non-enhanceable by bs253

Mer3 has leg phenotype, non-enhanceable by bs253

Mer3 has head phenotype, non-enhanceable by net107

Mer3 has leg phenotype, non-enhanceable by net107

Mer3 has head phenotype, non-enhanceable by net383

Mer3 has leg phenotype, non-enhanceable by net383

Mer3 has phenotype, non-enhanceable by Df(2L)H20/+

Mer3 has phenotype, non-enhanceable by +/Df(2L)Mdh

Mer3 has phenotype, non-enhanceable by Df(2L)cact-255rv64/+

Mer3 has phenotype, non-enhanceable by Df(2R)cn88b/+

Mer3 has phenotype, non-enhanceable by Df(2R)en30/+

Mer3 has phenotype, non-enhanceable by Df(3R)Antp17/+

Mer3 has phenotype, non-enhanceable by Df(3R)Tl-P/+

Mer3 has phenotype, non-enhanceable by Df(3R)awd-KRB/+

Mer3 has phenotype, non-enhanceable by sbb04440

Mer3 has phenotype, non-enhanceable by sbbk00702

Mer3 has phenotype, non-enhanceable by Df(2L)net-PMF/+

Suppressed by
Statement
Reference

Mer3 has wing phenotype, suppressible | partially by bs03267/bs[+]

Mer3 has wing phenotype, suppressible | partially by bs[+]/bs242

Mer3 has wing phenotype, suppressible | partially by bs[+]/bs253

Mer3 has wing phenotype, suppressible | partially by bs364/bs[+]

Mer3 has wing phenotype, suppressible | partially by net107/net[+]

Mer3 has wing phenotype, suppressible | partially by net[+]/net383

Mer3 has wing vein phenotype, suppressible by net383

NOT suppressed by
Statement
Reference

Mer3 has head phenotype, non-suppressible by bs242

Mer3 has leg phenotype, non-suppressible by bs242

Mer3 has phenotype, non-suppressible by +/Df(2L)Mdh

Mer3 has phenotype, non-suppressible by Df(2L)cact-255rv64/+

Mer3 has phenotype, non-suppressible by Df(2R)cn88b/+

Mer3 has phenotype, non-suppressible by Df(2R)en30/+

Mer3 has phenotype, non-suppressible by Df(3R)Antp17/+

Mer3 has phenotype, non-suppressible by Df(3R)Tl-P/+

Mer3 has phenotype, non-suppressible by Df(3R)awd-KRB/+

Mer3 has phenotype, non-suppressible by sbb04440

Mer3 has phenotype, non-suppressible by sbbk00702

Mer3 has phenotype, non-suppressible by Df(2L)net-PMF/+

Mer3 has head phenotype, non-suppressible by bs253

Mer3 has leg phenotype, non-suppressible by bs253

Mer3 has head phenotype, non-suppressible by net107

Mer3 has leg phenotype, non-suppressible by net107

Mer3 has head phenotype, non-suppressible by net383

Mer3 has leg phenotype, non-suppressible by net383

Mer3 has phenotype, non-suppressible by Df(2L)H20/+

Additional Comments
Genetic Interactions
Statement
Reference

Mutant phenotype is dominantly modified by Df(2L)al, Df(2L)ast2, Df(2L)JS32, Df(2L)cl-h3, Df(2L)30A-C, Df(2L)J39, Df(2R)M41A, Df(2R)nap1, Df(2R)pk78s, Df(2R)44CE, Df(2R)en-A, Df(2R)or-BR6, Df(3L)HR119, Df(3L)ZN47, Df(3L)brm11, Df(3L)VW3, Df(3R)T-32, Df(3R)ry615, Df(3R)P14, Df(3R)Cha7 or Df(3R)e-N19. Df(2L)C144 or In(2R)bwVDe2LCyR dominantly enhances the Mer3 phenotypes, including reduction in size of the eye and formation of aberrant cuticle, bristles and outgrowths. The increase in the size of the wing blade seen in Mer3/Y flies is partially suppressed by bs253/+, bs242/+, bs364/+, bs03267/+, net107/+ or net383/+.

dpphr56 dominantly enhances the phenotype of Mer3 hemizygotes; the eye is smaller and more roughened and there is increased expansion of peripheral head cuticle. Numerous interommatidial bristle duplications are seen.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Rescued by
Comments
Images (1)
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (11)