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General Information
Symbol
Dmel\exUAS.cBa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Boedigheimer
FlyBase ID
FBal0062855
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-ex, UAS-expanded
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Near full length ex cDNA expression is governed by UASt regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expressing exUAS.cBa under the control of Scer\GAL4GMR.PU induces ectopic cell apoptosis (in TUNEL assay) in the wing disc posterior compartment.

Ectopic expression of exScer\UAS.cBa under the control of Scer\GAL4nub.PU results in severely undergrown wings and expression under Scer\GAL4GMR.PU results in loss of eye tissue and smaller eyes in adult flies.

Expression of exScer\UAS.cBa under the control of Scer\GAL4C587 has no apparent germarium phenotype.

Expression of exScer\UAS.cBa under the control of Scer\GAL4GMR.PF induces a stripe of dying cells posterior to the morphogenetic furrow in third instar eye discs.

Expression of exScer\UAS.cBa under the control of Scer\GAL432B results in a substantial reduction in wing size.

Cells in the wing disc expressing exScer\UAS.cBa under the control of Scer\GAL4hh-Gal4 grow very poorly.

Expression of exScer\UAS.cBa under the control of Scer\GAL4hh-Gal4 from the early third larval instar onwards (the temperature sensitive Scer\GAL80ts.αTub84B allele is used to restrict expression before this stage) does not disrupt the apical F-actin network in the wing disc.

Expression of exUAS.cBa under the control of Scer\GAL4dpp.PU causes slight undergrowth of the expressing region of the wing disc in the larva. Expression of exUAS.cBa under the control of Scer\GAL4nub.PU causes an extreme reduction in the size of the adult wing.

Expression of exScer\UAS.cBa in the germline, under the control of Scer\GAL4nos.UTR.T:Hsim\VP16, does not result in germ-cell over-proliferation, or any alteration of cell morphology or loss of fertility.

Expression of exScer\UAS.cBa in the somatic cells of the testis, under the control of Scer\GAL4C587, results in non-autonomous over-proliferation of spermatogonial cells.

Expression of exScer\UAS.cBa, under the control of Scer\GAL4GMR.PF at 25oC elicits a severe reduction in eye size.

Larval wing disc clones of cells overexpressing exScer\UAS.cBa under the control of Scer\GAL4Scer\FRT.Act5C are normally composed of only a few cells and are lost over time.

exScer\UAS.cBa; Scer\GAL4GMR.PF flies have severely reduced and mispatterned eyes. At midpupal stages, the developing retinas in these animals are disorganised with very reduced numbers of inter-ommatidial cells.

Increased levels of cell death are seen in the eye discs of these animals at late third instar.

Expression of exScer\UAS.cBa under the control of Scer\GAL4hs.2sev results in a small, externally roughened eye. Expression of exScer\UAS.cBa under the control of Scer\GAL4dpp.blk1 results in a reduction of the L3-L4 intervein area. Flies expressing exScer\UAS.cBa under the control of Scer\GAL4mir-278-Gal4KI are lean, measured as the normalised ratio of total body triglycerides to total protein.

Overexpression of exScer\UAS.cBa during eye development, under the control of Scer\GAL4GMR.PF severely disrupts eye development and results in small and rough eyes.

Expression of exScer\UAS.cBa under the control of Scer\GAL4hs.2sev results in smaller eyes than normal and roughening and blistering of the eye.

Expression of exScer\UAS.cBa under the control of Scer\GAL4ey.PH leads to adult eyes that are often bulged and misshapened, and reduced in the ventral region. Mild eye roughening is seen. Mild patterning defects in the eye are seen, primarily affecting the position of the equator. Expression of exScer\UAS.cBa under the control of Scer\GAL432B, leads to external roughening of the eye, reflected ommatidial rotations and a loss of pigment cells are seen. Expression of exScer\UAS.cBa under the control of Scer\GAL4GMR.PF leads to results in severe defects in eye development - adult eyes are blistered over the entire surface and are reduced in size. Expression of exScer\UAS.cBa under the control of Scer\GAL4hs.2sev results in severe defects in eye development - adult eyes are blistered and reduced in size. Blistering is visible in anterior regions but morphology is relatively normal in posterior ommatidial rows. The blistered areas show highly disorganised ommatidia. The non-blistered areas exhibit a lack of pigment cells, defects in photoreceptor recruitment and defects in planar polarity. Expression of exScer\UAS.cBa under the control of Scer\GAL4ey.PH produces defects in the eye disc. Discs are not flat and small relative to the antennal portions of the same disc. However, ommatidial preclusters look fairly well ordered. Expression of exScer\UAS.cBa under the control of Scer\GAL432B in eye discs leads to normal alignment of photoreceptor precursor pairs, but misrotated clusters are observed even in more anterior rows. Rotation defects are also prominent in anterior rows if exScer\UAS.cBa is driven by Scer\GAL4hs.2sev or Scer\GAL4GMR.PF. In Scer\GAL4hs.2sev, exScer\UAS.cBa discs one of the R3/R4 precursor pair is occasionally missing. The number of accessory cells which normally surround the photoreceptors appears to be greatly reduced. Organisation of ommatidia is severely disrupted and in many cases ommatidial preclusters have too few cone cell nuclei. Expression of exScer\UAS.cBa under the control of Scer\GAL4hs.2sev, Scer\GAL4GMR.PF or Scer\GAL4ey.PH causes an increase in apoptosis (as indicated by incorporation of Acridine Orange) in the eye disc. Expression of exScer\UAS.cBa under the control of Scer\GAL4bi-omb-Gal4 results in increased cell death in the wing pouch and parts of the leg disc. producing adult flies with severely reduced wings with patterning defects including loss of vein material and disruption of the margin. Legs appear to be fragile and twisted. Expression of exScer\UAS.cBa under the control of Scer\GAL4Bx-MS1096 results in small, unpatterned wings and misshapen legs.

When expression is driven by Scer\GAL432B in an otherwise wild type background the wing area is severely reduced. There is greater size reduction between L2 and L3 than anywhere else. Reduction in size is due to reduced cell number, not size. Some wings show ectopic anterior crossveins, with delta-like wing veins and notched wing margins appearing at lower frequencies.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
NOT Enhanced by
Statement
Reference

Scer\GAL4hs.2sev, exUAS.cBa has visible phenotype, non-enhanceable by Cdk43/Cdk4[+]

Suppressed by
Statement
Reference
NOT suppressed by
Suppressor of
NOT Suppressor of
Phenotype Manifest In
Enhanced by
Statement
Reference
NOT Enhanced by
Statement
Reference

Scer\GAL4hs.2sev, exUAS.cBa has eye phenotype, non-enhanceable by Cdk43/Cdk4[+]

Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference

Scer\GAL4hs.2sev, exUAS.cBa has eye phenotype, non-suppressible by Cdk43/Cdk4[+]

Suppressor of
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

The severely undergrown wing phenotype seen in adult flies expressing exScer\UAS.cBa under the control of Scer\GAL4nub.PU can be strongly alleviated by co-expression of ZyxScer\UAS.cGa.

The small eye phenotype seen in adult flies expressing exScer\UAS.cBa under the control of Scer\GAL4GMR.PU can be strongly alleviated by co-expression of ZyxScer\UAS.T:Myr-Src42A.

The severely undergrown wing phenotype seen in adult flies expressing exScer\UAS.cBa under the control of Scer\GAL4nub.PU cannot be suppressed by co-expression of ZyxΔ208-231.Scer\UAS : the wings of flies expressing both exScer\UAS.cBa and ZyxΔ208-231.Scer\UAS are even smaller than those of animals expressing exScer\UAS.cBa alone, the wing blade being lost altogether.

Expression of sdJF02514 (co-expressed with Dcr-2Scer\UAS.cDa) significantly suppresses the morphogenetic furrow cell death seen when exScer\UAS.cBa is expressed under the control of Scer\GAL4GMR.PF.

Expression of TgiGD7081 (co-expressed with Dcr-2Scer\UAS.cDa) significantly suppresses the morphogenetic furrow cell death seen when exScer\UAS.cBa is expressed under the control of Scer\GAL4GMR.PF.

Co-expression of exScer\UAS.cBa partially suppresses the F-actin accumulation caused by expression of cpadsRNA.Scer\UAS.cFa under the control of Scer\GAL4hh-Gal4 from the early third larval instar onwards (the temperature sensitive Scer\GAL80ts.αTub84B allele is used to restrict expression before this stage).

Expression of ex[Scer\UAS.cBa] under the control of Scer\GAL4Act.PU suppresses the head overgrowth seen in mopsfv3 Df(3L)H99 mutant clones (generated in a minute background).

Expression of exScer\UAS.cBa under the control of Scer\GAL4C855a suppresses the increase in volume of the optic lobe neuroepithelium which is seen in l(3)mbtGM76 homozygotes.

Co-expression of exScer\UAS.cBa in clones expressing ykiS168A.Scer\UAS.T:SV5\V5, wtsScer\UAS.T:Hsap\MYC and hpoScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4Act5C.PI decreases the size and number of clones recovered in the wing disc.

Clones co-expressing ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 exScer\UAS.cBa, wtsScer\UAS.T:Hsap\MYC and hpoScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4Act5C.PI in the wing disc are large and round.

Clones of cells in the wing or eye disc in which wtsScer\UAS.T:Hsap\MYC, hpoScer\UAS.T:Zzzz\FLAG and exScer\UAS.cBa are over-expressed together with ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 generally overgrow compared to control clones, but can be less overgrown than ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5-expressing clones alone. This is most evident in the wing pouch.

The overgrowth and folding of the eye disc induced by Scer\GAL4GMR.PU-mediated expression of ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 is largely suppressed by co-expression of wtsScer\UAS.T:Hsap\MYC, hpoScer\UAS.T:Zzzz\FLAG and exScer\UAS.cBa.

The overgrowth and folding of the eye disc induced by Scer\GAL4GMR.PU-mediated expression of ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 is not suppressed by co-expression of exScer\UAS.cBa alone, nor by co-expression of wtsScer\UAS.T:Hsap\MYC and exScer\UAS.cBa together.

The overgrowth and folding of the eye disc induced by Scer\GAL4GMR.PU-mediated expression of ykiS111A.S168A.S250A.Scer\UAS.T:SV5\V5 is partly suppressed by co-expression of hpoScer\UAS.T:Zzzz\FLAG and exScer\UAS.cBa together.

Eye clones of ft8 misexpressing exScer\UAS.cBa, under the control of Scer\GAL4GMR.PF at 25oC exhibit small eyes, indicating that the small-eye phenotype produced by misexpressing exScer\UAS.cBa is epistatic to the eye size resulting from ft8.

In eye-discs mosaic for ft8, the expression exScer\UAS.cBa under the control of Scer\GAL4GMR.PF induces apoptosis as in wild-type tissue.

A wtsx1 mutant background allows recovery of larval wing disc clones expressing exScer\UAS.cBa under the control of Scer\GAL4Scer\FRT.Act5C.

The loss of inter-ommatidial cells in the developing retinas of exScer\UAS.cBa; Scer\GAL4GMR.PF animals at the mid-pupal stage is completely suppressed by hpo42-47/hpo42-47 or in wtsx1/wtsx1 somatic clones. In each case, the resulting retinas have an excess of inter-ommatidial cells as do wtsx1/wtsx1 or hpo42-47/hpo42-47 animals. Heterozygosity for wtsx1 partially suppresses the reduction in size and patterning defects seen in the eyes of exScer\UAS.cBa; Scer\GAL4GMR.PF animals.

The increase in cell death the late third instar eye discs of exScer\UAS.cBa; Scer\GAL4GMR.PF animals is largely suppressed by wtsx1/wtsx1 or hpo42-47/hpo42-47.

Overexpression of exScer\UAS.cBa in the developing eyes of ft422 mutant heads, under the control of Scer\GAL4GMR.PF results in small and rough eyes, very similar to the overexpression of exScer\UAS.cBa in a wild-type background, indicating that exScer\UAS.cBa expression suppresses the ft422 mutant eye phenotype. ft422 mutant phenotypes, such as extra vibrassae, are still observed in the head cuticle, where exScer\UAS.cBa is not overexpressed (under the control of Scer\GAL4GMR.PF).

Co-expression of banEP3622 almost completely suppresses the eye phenotype caused by expression of exScer\UAS.cBa under the control of Scer\GAL4hs.2sev. The eye phenotype caused by expression of exScer\UAS.cBa under the control of Scer\GAL4hs.2sev is enhanced by Df(3L)banΔ1/+. Co-expression of both CycDScer\UAS.cMa and Cdk4Scer\UAS.cMa in animals expressing exScer\UAS.cBa under the control of Scer\GAL4hs.2sev increases the overall size of the eye but has little effect on the roughness and blistering. The eye phenotype caused by expression of exScer\UAS.cBa under the control of Scer\GAL4hs.2sev is not altered by Cdk43/+.

Addition of BacA\p35Scer\UAS.cHa to exScer\UAS.cBa, Scer\GAL4hs.2sev flies rescues the phenotypes seen in exScer\UAS.cBa, Scer\GAL4hs.2sev flies alone. Externally the eye is much smoother and in sections most of the cell loss is rescued, although occasional photoreceptor and pigment cell loss is evident. Defects in planar polarity are still prominent.

Xenogenetic Interactions
Statement
Reference

Co-expression of BacA\p35Scer\UAS.cHa allows recovery of larval wing disc clones expressing exScer\UAS.cBa under the control of Scer\GAL4Scer\FRT.Act5C.

The loss of inter-ommatidial cells in the developing retinas of exScer\UAS.cBa; Scer\GAL4GMR.PF animals at the mid-pupal stage is only partially suppressed by BacA\p35Scer\UAS.cHa.

Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
exScer\UAS.cBa
exUAS.cBa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Boedigheimer
Secondary FlyBase IDs
    References (23)