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General Information
Symbol
Dmel\pumbem
Species
D. melanogaster
Name
FlyBase ID
FBal0083062
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

The P{lacW} transposon in inserted between exons 8 and 9 on the pum transcription unit, about 54kb from exon 8 and 75 kb from exon 9.

P{lacW} element in the intron between exons 8 and 9 of pum.

Insertion components
P{lacW}pumbem
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

No retinal phenotypes are seen in pumbem mutant flies.

In pumbem aCC/RP2 neurons, the magnitude of the persistent component of voltage-gated Na+ current is significantly increased relative to controls, while the transient component is significantly different. Endogenous membrane excitability in pumbem aCC/RP2 neurons, determined by injection of constant current, is increased relative to controls.

pumbem/pum7 and pumbem/pum9 transheterozygotes exhibit a significantly faster long term facilitation (LTF) at the neuromuscular junction than seen in wild-type. Larvae also respond to nerve stimuli with excitatory junctional potentials (ejp's) about 95% of time (as compared to about 80% in controls) in low Ca2+ (0.15mM) conditions. At 0.10mM Ca2+ an ejp response is seen about 85% of the time (as compared to about 35% in controls).

Flies exhibit greatly reduced coordination, flight ability and fertility. Mutation affects synaptic transmission at the larval neuromuscular junction via an affect on excitability in the motor neuron: the mutation acts presynaptically to cause increased transmitter release. Larvae exhibit a more rapid onset of augmentation than pum+ larvae.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressor of
Statement
Reference

pumbem/pum[+] is a suppressor of retina phenotype of Hsap\ATXN182Q.UAS, Scer\GAL4GMR.PU

Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

One copy of pumbem suppresses the retinal phenotype seen when Hsap\HTT128Q.1-336.Scer\UAS is expressed under the control of Scer\GAL4GMR.PU.

One copy of pumbem suppresses the retinal phenotype seen when Hsap\ATXN182Q.Scer\UAS is expressed under the control of Scer\GAL4GMR.PU.

Complementation and Rescue Data
Comments

Complementation tested against pumbem female sterility phenotype. pum03203, pum7, pum9 were also tested against the pumbem gravitaxis and flight defects, and found not to complement. pum7, pum9 were also tested against the pumbem long term facilitation (LTF) defects and found not to complement.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

Reversion tests reveal P{lacW} insertion is responsible for the mutant phenotype.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (7)