FlyBase Allele Report: Dmel\Ras85D[ΔC40B]

Type II neuroblast lineage MARCM clones mutant for Ras85D^ΔC40B do not display any significant changes in the clone morphology, size or the number of progenitor cells it contains compared to wild-type clones.

(Rives-Quinto et al., 2017)

Although the initial growth of Ras85D^ΔC40B mutant intestinal stem cell clones is normal, their long-term proliferation is severely compromised. These clones do not grow even after the flies have recovered from Pseudomonas entomophila infection.

(Jiang et al., 2011)

6 days after clone induction, 100% of testes carry one or more marked homozygous CPC clones, with an average of 3.4 marked homozygous CPCs per testis (compared to 92% of testes carrying one or more marked wild-type CPC clones, with an average of 1.1 marked wild-type CPCs per testis).

(Singh et al., 2010)

Ras85D^ΔC40B mutant eye disc clones show defects in ommatidium development. Gaps and partially-formed rosettes are observed within the ommatidial array.

(Sung et al., 2010)

In Ras85D^ΔC40B mutant adult Malpighian tubule clones only a few differentiated renalcytes (RCs) are detected, reduced in number compared to control clones.

(Zeng et al., 2010)

Homozygous Ras85D^ΔC40B mutant adult midgut progenitor cells fail to proliferate during larval development.

(Jiang and Edgar, 2009)

Homozygous clones in the eye disc are much smaller than their wild-type twin spots.

(Tseng et al., 2007)

Ras85D^ΔC40B Minute clones in the eye disc fail to form the arcs and rosettes of cells in the morphogenetic furrow that are seen in wild-type eye discs.

(Brown et al., 2006)

Larval eye discs containing Ras85D^ΔC40B clones show a higher level of cell death than controls.

(Polesello et al., 2006)

Clones of Ras85D^ΔC40B generated in the developing eye are aberrantly small.

(Polesello et al., 2006)

Oocyte polarity and apical-basal polarity of posterior follicle cells (PFCs) is disrupted in Ras85D^ΔC40B mutant PFC clones.

(Poulton and Deng, 2006)

The initiation of mesoderm spreading is unaffected in homozygous embryos derived from homozygous female germline clones.

(Wilson et al., 2005)

Embryos derived from homozygous Ras85D^ΔC40B female germ line clones have 20-30% fewer pole cells by the cellularization stage, due to mitotic defects, when compared with wild-type embryos. These mutant embryos exhibit defective gastrulation and lack the posterior midgut primordium. The majority of pole cells are unable to enter the embryo at gastrulation and those that do enter the embryo appear abnormal in both directed migration and aggregation, leading to their random scattering in the embryonic cavity. Analysis of pole cell transplantations, in which pole cells from cellularization stage embryos derived from Ras85D^ΔC40B female germ line clones and wild-type fathers are transplanted into wild-type embryos shows that no Ras85D^ΔC40B pole cells are ever found in the gonad of the transplanted embryo but migrate to ectopic locations. These Ras85D^ΔC40B mutant pole cells disappear in late stage embryos, possibly due to cell death.

(Li et al., 2003)

In Ras85D^ΔC40B mutant somatic clones in the 3rd instar eye disc, photoreceptors R2-R5 fail to undergo or differentiation. Within Ras85D^ΔC40B mutant somatic clones there is a failure of G1 arrest in the furrow: all cells except R8s re-enter the cell cycle. However, none of these cells progress past G2.

(Yang and Baker, 2003)

Homozygous clones in the follicle cells are smaller than control clones and are detected at a lower frequency 6 days after clone induction. Large follicle stem cell clones are not recovered 10 days after clone induction. 16% of stage 14 eggshells laid by mosaic females (containing homozygous follicle cell clones) 5-7 days after clone induction have dorsal appendage defects. The defects include complete lack of dorsal appendages (15%), a small "nub" of extra chorion material next to two normal dorsal appendages (4.5%), short dorsal appendages (11%), fragmented dorsal appendages (11%), one dorsal appendage replaced by a nub of chorion material (42%), both dorsal appendages replaced by a nub of chorion material (12%) and fragmented dorsal appendages plus an extra nub of material (4.5%). Mutant cells are not found amongst the migrating follicle cells that give rise to dorsal appendages. Aberrant nubs and fragments of dorsal appendages are not made by mutant cells, but by wild-type cells adjacent to mutant clones. Egg chambers with dorsal anterior mutant clones show gross disruption of the apical morphology of the dorsal appendage primordia at stage 12.

(James et al., 2002)

Homozygous somatic clones in the wing, autonomously prevent wing vein formation.

(Roch et al., 2002)

Homozygous embryos show loss of VA2 muscle precursor cells (only 9% of hemisegments contain VA2 precursor cells).

(Johnson Hamlet and Perkins, 2001)

Excess R8 cell precursors are seen in homozygous clones in the eye disc.

(Yang and Baker, 2001)

A gradual decline over time in the number of homozygous clones present compared to wild-type sister clones is seen in imaginal discs. There is also a decline in the area of the surviving homozygous clones compared to wild-type clones. The homozygous clones contain many pyknotic nuclei. Relatively large homozygous clones containing 40-80 cells are seen even 72 hours after induction, but at a low frequency. Homozygous clones (which are RpS3⁺) induced in discs which are RpS3^-/+ have increased survival rates and are larger than homozygous clones induced in a wild-type background.

(Prober and Edgar, 2000)

Embryos derived from homozygous female germline clones develop abdominal segment A8 in 20.3% of cases and filzkorper in 0.4% of cases.

(Lu and Li, 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Ras85D^ΔC40B has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by Stat92E⁰⁶³⁴⁶

(Li et al., 2003)

Suppressed by

Statement

Reference

Ras85D^ΔC40B has increased cell death | somatic clone phenotype, suppressible by Rassf^X36

(Polesello et al., 2006)

Ras85D^ΔC40B has decreased cell size | somatic clone phenotype, suppressible by Rassf^X36

(Polesello et al., 2006)

Ras85D^ΔC40B has abnormal cell polarity | somatic clone | cell non-autonomous phenotype, suppressible by Scer\GAL4^Act.PU/Dg^RNAi.UAS

(Poulton and Deng, 2006)

Ras85D^ΔC40B has abnormal cell polarity | somatic clone | cell autonomous phenotype, suppressible by Scer\GAL4^Act.PU/Dg^RNAi.UAS

(Poulton and Deng, 2006)

Enhancer of

Statement

Reference

Ras85D^ΔC40B is an enhancer of abnormal mitotic cell cycle | maternal effect phenotype of Stat92E⁰⁶³⁴⁶

(Li et al., 2003)

NOT Enhancer of

Statement

Reference

Ras85D^ΔC40B/Ras85D^ΔC40B is a non-enhancer | somatic clone of abnormal cell number | third instar larval stage | somatic clone phenotype of cno^R2

(Rives-Quinto et al., 2017)

Ras85D^ΔC40B is a non-enhancer of visible phenotype of upd1^GMR.PB

(Bach et al., 2003)

Suppressor of

Statement

Reference

Ras85D^ΔC40B/Ras85D^ΔC40B is a suppressor | partially | somatic clone of abnormal neuroanatomy | somatic clone | third instar larval stage phenotype of cno^R2

(Rives-Quinto et al., 2017)

Ras85D^ΔC40B/Ras85D^ΔC40B is a suppressor | somatic clone of decreased cell size | somatic clone | third instar larval stage phenotype of cno^R2

(Rives-Quinto et al., 2017)

Ras85D^ΔC40B is a suppressor of visible phenotype of Ret^MEN2B.GMR

(Read et al., 2005)

NOT Suppressor of

Statement

Reference

Ras85D^ΔC40B is a non-suppressor of abnormal mitotic cell cycle | somatic clone phenotype of Delta^RevF10

(Baonza and Freeman, 2005)

Ras85D^ΔC40B is a non-suppressor of visible phenotype of upd1^GMR.PB

(Bach et al., 2003)

Other

Statement

Reference

Delta^RevF10, Ras85D^ΔC40B has abnormal neuroanatomy | somatic clone phenotype

(Baonza and Freeman, 2005)

Delta^RevF10, Ras85D^ΔC40B has decreased occurrence of cell division phenotype

(Baonza and Freeman, 2005)

Raf^Su2, Ras85D^ΔC40B has lethal phenotype

(Li et al., 2000)

Raf^Su2, Ras85D[+]/Ras85D^ΔC40B has lethal phenotype

(Li et al., 2000)

Phenotype Manifest In

Enhanced by

Statement

Reference

Ras85D^ΔC40B has primordial germ cell | maternal effect phenotype, enhanceable by Stat92E⁰⁶³⁴⁶

(Li et al., 2003)

Suppressed by

Statement

Reference

Ras85D^ΔC40B has oocyte | somatic clone | cell non-autonomous phenotype, suppressible by Scer\GAL4^Act.PU/Dg^RNAi.UAS

(Poulton and Deng, 2006)

Ras85D^ΔC40B has main body follicle cell | somatic clone | cell autonomous phenotype, suppressible by Scer\GAL4^Act.PU/Dg^RNAi.UAS

(Poulton and Deng, 2006)

Ras85D^ΔC40B has wing vein | somatic clone phenotype, suppressible by cic^D49

(Roch et al., 2002)

Ras85D^ΔC40B has muscle cell of ventral acute muscle 2 | precursor phenotype, suppressible by Scer\GAL4^twi.PG/csw^{UAS.Tag:Myr(Src64B)}

(Johnson Hamlet and Perkins, 2001)

Ras85D^ΔC40B has imaginal disc | somatic clone phenotype, suppressible by Scer\GAL4^en-e16E/BacA\p35^UAS.cHa

(Prober and Edgar, 2000)

Ras85D^ΔC40B has embryonic abdominal segment 8 phenotype, suppressible by Src42A^7-4

(Lu and Li, 1999)

Ras85D^ΔC40B has filzkorper phenotype, suppressible by Src42A^7-4

(Lu and Li, 1999)

NOT suppressed by

Statement

Reference

Ras85D^ΔC40B has adult Malpighian tubule | somatic clone phenotype, non-suppressible by scrib¹

(Zeng et al., 2010)

Ras85D^ΔC40B has adult Malpighian tubule | somatic clone phenotype, non-suppressible by sav³

(Zeng et al., 2010)

Ras85D^ΔC40B has ommatidial precursor cluster | somatic clone phenotype, non-suppressible by Scer\GAL4^GMR.PF/ato^UAS.cJa

(Brown et al., 2006)

Enhancer of

Statement

Reference

Ras85D^ΔC40B is an enhancer of primordial germ cell | maternal effect phenotype of Stat92E⁰⁶³⁴⁶

(Li et al., 2003)

Ras85D^ΔC40B is an enhancer of phenotype of csw^lf

(Firth et al., 2000)

Ras85D^ΔC40B is an enhancer of eye phenotype of hid^GMR.PG

(Bergmann et al., 1998)

Ras85D^ΔC40B is an enhancer of ommatidium phenotype of hid^GMR.PG

(Bergmann et al., 1998)

NOT Enhancer of

Statement

Reference

Ras85D^ΔC40B is a non-enhancer of eye phenotype of upd1^GMR.PB

(Bach et al., 2003)

Suppressor of

Statement

Reference

Ras85D^ΔC40B is a suppressor of eye phenotype of Ret^MEN2B.GMR

(Read et al., 2005)

Ras85D^ΔC40B is a suppressor of embryonic/first instar larval cuticle | maternal effect phenotype of tor^12D

(Li and Li, 2003)

NOT Suppressor of

Statement

Reference

Ras85D^ΔC40B is a non-suppressor of eye disc | somatic clone phenotype of Delta^RevF10

(Baonza and Freeman, 2005)

Ras85D^ΔC40B is a non-suppressor of eye phenotype of upd1^GMR.PB

(Bach et al., 2003)

Ras85D^ΔC40B is a non-suppressor of phenotype of Src42A^Su(Raf)1-1

(Zhang et al., 1999)

Other

Statement

Reference

Ras85D^ΔC40B, Stat92E³⁹⁷ has intestinal stem cell phenotype

(Jiang et al., 2011)

Ras85D^ΔC40B, Stat92E³⁹⁷ has enterocyte phenotype

(Jiang et al., 2011)

Ras85D^ΔC40B, cic^Q474X has ommatidium | somatic clone phenotype

(Tseng et al., 2007)

Ras85D^ΔC40B, cic^Q474X has photoreceptor neuron | somatic clone phenotype

(Tseng et al., 2007)

Delta^RevF10, Ras85D^ΔC40B has eye disc | somatic clone phenotype

(Baonza and Freeman, 2005)

Additional Comments

Genetic Interactions

Statement

Reference

The reduced number of progenitor cells in cno^R2 clones is not significantly affected by combination with Ras85D^ΔC40B, but the morphology and size of the clones and the neuroblasts within them is more similar to wild-type.

(Rives-Quinto et al., 2017)

Ras85D^ΔC40B, Stat92E³⁹⁷ double mutant intestinal stem cell clones are smaller than adjacent cells and lack enterocyte cells.

(Jiang et al., 2011)

Ras85D^ΔC40B, scrib¹ double mutant adult Malpighian tubule clones display only a few differentiated renalcytes (RCs), like the single Ras85D^ΔC40B mutant phenotype.

Ras85D^ΔC40B, sav³ double mutant adult Malpighian tubule clones display only a few differentiated renalcytes (RCs), like the single Ras85D^ΔC40B mutant phenotype.

(Zeng et al., 2010)

Ras85D^ΔC40B, cic^Q474X double mutant clones in the eye disc are typically larger than their wild-type twin spots, as occurs in cic^Q474X single mutant clones. In the adult, double mutant clones are seen, but the mutant tissue lacks the normal ommatidial architecture.

Ras85D^ΔC40B Tsc1^unspecified double mutant clones in the eye discs are no larger than Ras85D^ΔC40B single mutant clones.

(Tseng et al., 2007)

Expressing ato^Scer\UAS.cJa under the control of Scer\GAL4^GMR.PF fails to suppress the morphogenetic furrow phenotype of Ras85D^ΔC40B clones.

(Brown et al., 2006)

Larval eye discs containing Ras85D^ΔC40B, Rassf^X36 double mutant clones show a partial rescue of the increased cell death phenotype observed in discs with Ras85D^ΔC40B single mutant clones.

(Polesello et al., 2006)

Double-mutant Ras85D^ΔC40B, Rassf^X36 clones in the eye show a rescue of the growth defect seen in single mutant Ras85D^ΔC40B clones.

(Polesello et al., 2006)

The oocyte polarity defects seen when Ras85D^ΔC40B mutant clones are made in the posterior follicle cell layer are partially rescued by expression of Dg^{dsRNA.Scer\UAS} in the clone using Scer\GAL4^Act and the 'MARCM' technique.

The polarity defects of posterior follicle cells (PFCs) seen in Ras85D^ΔC40B mutant PFC clones are partially rescued by expression of Dg^{dsRNA.Scer\UAS} in the clone using Scer\GAL4^Act and the 'MARCM' technique.

(Poulton and Deng, 2006)

Neural differentiation and the second mitotic wave are blocked in clones of cells in the eye disc simultaneously mutant for Dl^RevF10 and Ras85D^ΔC40B in a Minute/+ background: mutant cells in the position of the second mitotic wave do not enter S phase and do not undergo mitosis, as in Dl^RevF10 clones.

(Baonza and Freeman, 2005)

Embryos derived from double mutant Stat92E⁰⁶³⁴⁶; Ras85D^ΔC40B female germ line clones have 54% fewer pole cells by the cellularization stage than wild-type embryos, which is a greater reduction than is seen in embryos derived from either single mutant female germline clone. Pole cells of these double mutant embryos appear slighlty larger than wild-type cells, consistent with their slower mitotic division rate.

(Li et al., 2003)

Within Ras85D^ΔC40B mutant clones in the eye disc in a BacA\p35^GMR.PH background, caspase activation is seen posterior to column 5, relative to the morphogenetic furrow. There is no increase in caspase activation outside of these clones.

(Yang and Baker, 2003)

The addition of cic^D49 suppresses the loss of wing vein phenotype seen in Ras85D^ΔC40B clones.

(Roch et al., 2002)

Expression of csw::Src64B^{src90.Scer\UAS} under the control of Scer\GAL4^twi.PG partially suppresses the loss of VA2 muscle precursor cells seen in Ras85D^ΔC40B embryos (21% of hemisegments have VA2 cells).

(Johnson Hamlet and Perkins, 2001)

Expression of BacA\p35^Scer\UAS.cHa under the control of Scer\GAL4^en-e16E increases the survival of homozygous Ras85D^ΔC40B clones in the imaginal disc from 2% to 86% (at 72 hours after induction of the clone). The size of the clones is also increased compared to their wild-type sister clones, although there is still a large size difference between them.

(Prober and Edgar, 2000)

A higher proportion of embryos derived from homozygous female germline clones develop abdominal segment A8 (52.9%) or filzkorper (12.4%) if the females are also carrying Src42A^7-4.

(Lu and Li, 1999)

Does not suppress the ability of Src42A^Su(phl)1-1 to suppress the lethality of phl¹/Y flies.

(Zhang et al., 1999)

Embryos derived from phl^Su3, Ras85D^ΔC40B double mutant germ cells die during embryogenesis with cuticles indistinguishable from those of Ras85D^ΔC40B mutants.

(Li et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Ras85D^ΔC40B is rescued by Ras85D^UAS.cKa/Scer\GAL80^ts.αTub84B/Scer\GAL4^αTub84B.PL

(Sung et al., 2010)

Ras85D^ΔC40B is rescued by Ras85D^V12.UAS/Scer\GAL80^ts.αTub84B/Scer\GAL4^αTub84B.PL

(Sung et al., 2010)

Ras85D^ΔC40B is rescued by Ras85D^{C186S.UAS.Tag:MYC}/Scer\GAL80^ts.αTub84B/Scer\GAL4^αTub84B.PL

(Sung et al., 2010)

Ras85D^ΔC40B is rescued by Ras85D^{12V,C186S.UAS.Tag:MYC}/Scer\GAL80^ts.αTub84B/Scer\GAL4^αTub84B.PL

(Sung et al., 2010)

Ras85D^ΔC40B is rescued by Scer\GAL4^en-e16E/Ras85D^UAS.cKa

(Prober and Edgar, 2000)

Partially rescued by

Ras85D^ΔC40B is partially rescued by Ras85D^{K187V.UAS.Tag:MYC}/Scer\GAL80^ts.αTub84B/Scer\GAL4^αTub84B.PL

(Sung et al., 2010)

Ras85D^ΔC40B is partially rescued by Scer\GAL80^ts.αTub84B/Scer\GAL4^αTub84B.PL/Ras85D^{Ktail6Q.UAS.Tag:MYC.Tag:CAAX(hKRAS)}

(Sung et al., 2010)

Ras85D^ΔC40B is partially rescued by Ras85D^{Ktail.UAS.Tag:PM(hKRAS).Tag:MYC}/Scer\GAL80^ts.αTub84B/Scer\GAL4^αTub84B.PL

(Sung et al., 2010)

Comments

Expression of Ras85D^Scer\UAS.cKa under the control of Scer\GAL4^αTub84B.PL in combination with Scer\GAL80^ts.αTub84B in eye disc clones rescues the ommatidial development defects of Ras85D^ΔC40B homozygous cells.

Expression of Ras85D^{Ktail.Scer\UAS.T:Hsap\CAAX.T:Hsap\MYC} under the control of Scer\GAL4^αTub84B.PL in combination with Scer\GAL80^ts.αTub84B in eye disc clones can partially rescue the ommatidial development defects of Ras85D^ΔC40B homozygous cells.

Expression of Ras85D^{K187V.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^αTub84B.PL in combination with Scer\GAL80^ts.αTub84B in eye disc clones can partially rescue the ommatidial development defects of Ras85D^ΔC40B homozygous cells.

Expression of Ras85D^{Ktail6Q.Scer\UAS.T:Hsap\MYC.T:Hsap\CAAX-6Q} under the control of Scer\GAL4^αTub84B.PL in combination with Scer\GAL80^ts.αTub84B in eye disc clones can partially rescue the ommatidial development defects of Ras85D^ΔC40B homozygous cells.

Expression of Ras85D^{C186S.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^αTub84B.PL in combination with Scer\GAL80^ts.αTub84B in eye disc clones rescues the ommatidial development defects of Ras85D^ΔC40B homozygous cells. Clones show an enhanced recruitment of supernumerary photoreceptor cells.

Expression of Ras85D^V12.Scer\UAS under the control of Scer\GAL4^αTub84B.PL in combination with Scer\GAL80^ts.αTub84B in eye disc clones rescues the ommatidial development defects of Ras85D^ΔC40B homozygous cells. Clones show hyper-recruitment of photoreceptors.

Expression of Ras85D^{12V,C186S.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^αTub84B.PL in combination with Scer\GAL80^ts.αTub84B in eye disc clones rescues the ommatidial development defects of Ras85D^ΔC40B homozygous cells. Clones show an enhanced recruitment of supernumerary photoreceptor cells.

(Sung et al., 2010)

The size and survival of homozygous Ras85D^ΔC40B clones in the imaginal disc is completely rescued by expression of Ras85D^Scer\UAS.cKa under the control of Scer\GAL4^en-e16E.

(Prober and Edgar, 2000)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (26)

Reported As

Symbol Synonym

Dras1^c40b

(Yagi et al., 1998)

Ras1^c40b

(Polesello et al., 2006)

Ras1^mat-

(Li et al., 2003)

Ras1^ΔC40B

(Li and Li, 2003, Li et al., 2003, Li et al., 2002, Li et al., 2000, Li et al., 1998, Li et al., 1997)

Ras1^ΔC40b

(Mora et al., 2013, Mishra et al., 2005, Zhang et al., 1999, Dominguez et al., 1998)

Ras1^Δc40b

(Jiang and Edgar, 2009, Lu and Li, 1999)

Ras85D^CΔ40b

(de Navascués and Modolell, 2007)

Ras85D^c40B

(Wilson et al., 2005)

Ras85D^ΔC40B

(Duncan et al., 2018, Suisse et al., 2017, Andreu et al., 2012, Avet-Rochex et al., 2012, Sung et al., 2010)

Ras85D^Δc40b

(Read et al., 2005)

Ras^C40b

(Zeng et al., 2010)

Ras^DeltaC40B

(Botella et al., 2003)

Ras^c40b

(Juhász et al., 2007, Polesello et al., 2006)

Ras^ΔC40B

(Zhang et al., 2024, Roch et al., 2002, Johnson Hamlet and Perkins, 2001)

Ras^ΔC40b

(Tseng et al., 2007, Poulton and Deng, 2006, James et al., 2002, Firth et al., 2000)

Ras^δC40b

(Zartman et al., 2008)

ras1^c40b

(Singh et al., 2010)

ras1^Δc40b

(Yang and Baker, 2003, Yang and Baker, 2001)

ras^C40B

(Bach et al., 2003)

ras^D40

(Brown et al., 2006, Baonza and Freeman, 2005)

ras^DC408

(Pascual et al., 2017)

ras^c40b

(Prober and Edgar, 2002, Prober and Edgar, 2000)

ras^Δ40

(Baonza and Freeman, 2005)

ras^Δc40b

(Jiang et al., 2011)

rasC40B

(Sun and Stathopoulos, 2018)

rasr^DC40b

(Bergmann et al., 1998)

Name Synonyms

Secondary FlyBase IDs

References (47)

Report Sections

Open Close