Q524term | ed-PA; Q524term | ed-PB; Q524term | ed-PC
Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
sensillum campaniformium & wing | supernumerary | somatic clone
Ubiquitous expression of ed::shgScer\UAS.cCa under the control of Scer\GAL4C-765 is unable to suppress the cell sorting phenotypes seen in ed1X5 mutant wing disc clones. An actomyosin cable still forms around the clone and the boundary between the mutant clone and neighbouring wild type cells remains smooth. Apical constriction of the clone cells is still seen.
Gripex36, ed1X5/+ embryos exhibit more severe VLM defects than Gripex36 embryos. In severe cases, the double mutants exhibit completely deranged somatic musculature, where muscle identification is no longer possible. Lateral transverse muscles are defective in Gripex36/Y; ed1X5/+ embryos, while these muscles appear normal in both single heterozygote.
Independent ed1X5, smo3 double mutant clones that originate in different compartments (as evidenced by ci or en expression) can fuse together to form composite clones which have roundish, smooth shapes. Within such composite clones, the characteristic segregation of 'anterior' and 'posterior' cells is maintained - they do not intermix.
Expression of EgfrDN.Scer\UAS.cBa under the control of Scer\GAL4ap-md544 partially suppresses the extra bristle phenotype seen in ed1X5/edslH8 flies. The number of macrochaetae/heminotum in animals expressing EgfrDN.Scer\UAS.cBa under the control of Scer\GAL4ap-md544 in a ed1X5/edslH8 background is - anterior+posterior notopleural: 2.15, presutural: 1.05, anterior supraalar: 0.80, posterior supraalar: 0, anterior postalar: 0.60, posterior postalar: 0, anterior+posterior dorsocentral: 1.30 and anterior+posterior scutellar: 2.25. N55e11/+ ; ed1X5/edslH8 adults show an almost complete absence of microchaetae on the notum. DlRevF10/+ increases the number of extra macrochaetae on the notum seen in ed1X5/edslH8 adults. H2/+ almost completely eliminates the extra macrochaetae seen on the notum of ed1X5/edslH8 animals, although the increased density of microchaetae seen in ed1X5/edslH8 flies is not reduced by H2/+. The low density of microchaetae on the notum seen in NMcd1/+ flies is not rescued by ed1X5/edslH8.
Ubiquitous expression of edScer\UAS.cBa under the control of Scer\GAL4C-765 rescues the cell sorting phenotypes seen in ed1X5 mutant wing disc clones. The boundary between the clone and the surrounding wild type cells is no longer smooth edged, instead appearing jagged as in wild type. Normal levels of shg are seen.