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General Information
Symbol
Dmel\ed1X5
Species
D. melanogaster
Name
FlyBase ID
FBal0086650
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Mutagen
    Nature of the Allele
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Nucleotide change:

    C4098668T

    Amino acid change:

    Q524term | ed-PA; Q524term | ed-PB; Q524term | ed-PC

    Reported amino acid change:

    ?524term

    Comment:

    Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference

    Amino acid replacement: ?524term.

    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease-implicated variant(s)
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In

    sensillum campaniformium & wing | supernumerary | somatic clone

    Detailed Description
    Statement
    Reference

    In mosaic third instar larval wing discs, ed[1X5] mutant clone cells are constricted when compared to the neighboring control cells.

    ed1X5 mutant eye clones (generated using the ey-FLP method) are overgrown compared with control clones.

    In ed1X5 mutant posterior follicle cell clones multiple layers are formed around the wild type oocyte, rather than the single layer of cells seen in wild type. Mislocalisation of the oocyte nucleus is also seen.

    ed1X5 mutant wing disc clones sort out from the surrounding wild type cells, exhibiting both rounded and smooth contours rather than the jagged border seen in controls. The wild type cells surrounding the clone assemble a ring of actomyosin cable at the interface and the clone cells themselves develop an apical constriction that is inversely proportional to the size of the clone. Similar apical constriction is seen in wild type cells when they are surrounded by a large number of ed1X5 mutant cells.

    Mosaic ommatidia containing wild-type photoreceptors and between one and four mutant cone cells misrotate.

    ed1X5 animals predominantly die as embryos although some develop into second instar

    larvae. In these larvae, defects in ventrolateral muscles and lateral transverse muscles are evident with both muscle groups forming aberrant projections and ectopic adhesion points. Consistent and prominent defects in muscle morphology can also be observed at the embryonic stage. The same defects are present in ed1X5/edslH8 transheterozygotes.

    Epidermal tendon cells are positioned correctly in ed1X5 mutants.

    ed1X5 mutant clones in the third instar wing disc exhibit round and smooth contours in contrast to clones of wild type cells that show wiggly shapes. Both large and small ed1X5 clones are simultaneously observed, and often straddle either the dorsal-ventral or anterior-posterior compartment boundary.

    When ed1X5 clones are small, the mutant cells have a reduced apical surface: this apical constriction continues through the levels of the adherens and septate junctions, ending at the plane just below the gap junctions. Hence, these ed1X5 cells adopt a bottle shape. In contrast, ed1X5 cells within larger mutant clones (over hundreds of cells) have an apical surface more similar to those of wild type cells. Apposed ed1X5/ed1X5 and ed1X5/+ cells at the border of mutant clones are enlarged and adopt a rectangular shape, elongating towards the mutant clone itself.

    Large ed1X5 clones that are close to or touch the dorsal-ventral boundary of the wing disc displace the boundary towards the mutant clone. In contrast, ed1X5 clones that straddle this boundary do not overtly distort it, although the boundary can be less smooth within the clone.

    Adult wings bearing ed1X5 clones display rounded areas with altered patterns of trichomes. When these areas are small, the density of trichomes is increased and/or the pointing of trichomes is altered, while larger areas show these effects to a lesser or no extent. Appropriately located putative clones can develop extra sensilla campaniformia, extra wing vein material or a partial loss of wing vein. A crease of cuticle often surrounds clones, especially small ones.

    The number of macrochaetae/heminotum in ed1X5/edslH8 mutants is - anterior+posterior notopleural: 2.10, presutural: 1.28, anterior supraalar: 1.13, posterior supraalar: 1.05, anterior postalar: 1.80, posterior postalar: 1.85, anterior+posterior dorsocentral: 2.80 and anterior+posterior scutellar: 2.63. The density of microchaetae on the notum is also increased in these flies. Homozygous clones in imaginal wing discs are much smaller than wild-type twin clones, and many twins have no associated mutant clone. Within mutant clones, extra sensory organ precursor (SOP) cells are detected near extant SOPs. These clones give rise to an extra macrochaeta in the adult. Homozygous clones in the wing disc induced a Minute background are viable and include extra SOPs when they include regions from which extant SOPs arise. These clones give rise to groups of macrochaetae or areas of increased density of microchaetae in the adult.

    33% of ommatidia of ed1X5/edslH8 flies have extra R7-like cells. 26% of ommatidia have extra outer photoreceptors and 6% have fewer than normal outer photoreceptors. Mystery cells are transformed into neuronal photoreceptor cells. 69% of ommatidia have 5-6 instead of 4 cone cells, and 10% have three primary pigment cells. ed1X5/edslH8 flies show an enlarged wing with extra wing vein material.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Enhanced by
    Statement
    Reference

    edslH8/ed1X5 has visible phenotype, enhanceable by DlRevF10/Dl[+]

    Suppressed by
    Statement
    Reference

    edslH8/ed1X5 has visible phenotype, suppressible | partially by H2/H[+]

    NOT suppressed by
    Enhancer of
    Statement
    Reference

    ed[+]/ed1X5 is an enhancer of visible phenotype of Egfr::tort4021E.hs.sev

    ed[+]/ed1X5 is an enhancer of visible phenotype of rhohs.PSt

    ed[+]/ed1X5 is an enhancer of visible phenotype of Ras85DV12.sev

    ed[+]/ed1X5 is an enhancer of visible | dominant phenotype of EgfrE3

    ed[+]/ed1X5 is an enhancer of visible phenotype of Raf::tor13D.hs.sev

    ed[+]/ed1X5 is an enhancer of visible phenotype of rlSem

    Suppressor of
    Statement
    Reference

    ed[+]/ed1X5 is a suppressor | partially of visible phenotype of Ras85DN17.sev

    ed[+]/ed1X5 is a suppressor of visible phenotype of aoshs.PF

    NOT Suppressor of
    Statement
    Reference

    edslH8/ed1X5 is a non-suppressor of visible | dominant phenotype of NMcd1

    edslH8/ed1X5 is a non-suppressor of visible | dominant phenotype of NAx-M1

    Other
    Statement
    Reference
    Phenotype Manifest In
    Enhanced by
    Statement
    Reference

    edslH8/ed1X5 has macrochaeta | ectopic phenotype, enhanceable by DlRevF10/Dl[+]

    Suppressed by
    NOT suppressed by
    Statement
    Reference

    ed1X5 has wing disc | somatic clone phenotype, non-suppressible by shgαTub84B.PP

    edslH8/ed1X5 has microchaeta phenotype, non-suppressible by H2/H[+]

    Enhancer of
    Statement
    Reference

    ed[+]/ed1X5 is an enhancer of ommatidium phenotype of cnomis1/cno2

    ed[+]/ed1X5 is an enhancer of ommatidium phenotype of pnt1277/pntΔ88

    ed[+]/ed1X5 is an enhancer of ommatidium phenotype of fz20/fz19

    ed[+]/ed1X5 is an enhancer of ommatidium phenotype of dgo380

    ed[+]/ed1X5 is an enhancer of ommatidium phenotype of dsh1

    ed[+]/ed1X5 is an enhancer of ommatidium phenotype of stanfrz3

    ed[+]/ed1X5 is an enhancer of ventral longitudinal muscle phenotype of Gripex36

    ed[+]/ed1X5 is an enhancer of eye phenotype of Egfr::tort4021E.hs.sev

    ed[+]/ed1X5 is an enhancer of eye phenotype of rhohs.PSt

    ed[+]/ed1X5 is an enhancer of eye phenotype of Ras85DV12.sev

    ed[+]/ed1X5 is an enhancer of eye phenotype of EgfrE3

    ed[+]/ed1X5 is an enhancer of wing vein | increased number phenotype of EgfrE3

    ed[+]/ed1X5 is an enhancer of eye phenotype of Raf::tor13D.hs.sev

    ed[+]/ed1X5 is an enhancer of wing vein | increased number phenotype of rlSem

    ed1X5 is an enhancer of wing vein phenotype of EgfrE3

    ed1X5 is an enhancer of wing vein phenotype of rlSem

    ed1X5 is an enhancer of eye phenotype of Ras85DV12

    ed1X5 is an enhancer of eye phenotype of EgfrE1

    Suppressor of
    Statement
    Reference

    ed[+]/ed1X5 is a suppressor | partially of eye phenotype of Ras85DN17.sev

    edslH8/ed1X5 is a suppressor of ommatidium phenotype of pnt1277/pntΔ88

    edslH8/ed1X5 is a suppressor of ommatidium phenotype of aopACT.sev

    edslH8/ed1X5 is a suppressor of ommatidium phenotype of sina3/sina2

    ed[+]/ed1X5 is a suppressor of eye phenotype of aoshs.PF

    NOT Suppressor of
    Statement
    Reference

    edslH8/ed1X5 is a non-suppressor of macrochaeta phenotype of NAx-M1

    edslH8/ed1X5 is a non-suppressor of microchaeta phenotype of NMcd1

    Other
    Additional Comments
    Genetic Interactions
    Statement
    Reference

    Ubiquitous expression of ed::shgScer\UAS.cCa under the control of Scer\GAL4C-765 is unable to suppress the cell sorting phenotypes seen in ed1X5 mutant wing disc clones. An actomyosin cable still forms around the clone and the boundary between the mutant clone and neighbouring wild type cells remains smooth. Apical constriction of the clone cells is still seen.

    Expression of shgαTub84B.PP is unable to rescue the cell sorting phenotypes seen in ed1X5 mutant wing disc clones.

    ed1X5 dominantly enhances the phenotype of mis-rotation of ommatidia that is seen in dgo380, dsh1, stanfrz3, cno2/cnomis1 and pntΔ88/pnt1277 animals.

    ed1X5 dominantly enhances the phenotype of mis-rotation of ommatidia that is seen in fz19/fz20 animals, without affecting the chirality phenotype.

    Gripex36, ed1X5/+ embryos exhibit more severe VLM defects than Gripex36 embryos. In severe cases, the double mutants exhibit completely deranged somatic musculature, where muscle identification is no longer possible. Lateral transverse muscles are defective in Gripex36/Y; ed1X5/+ embryos, while these muscles appear normal in both single heterozygote.

    Independent ed1X5, smo3 double mutant clones that originate in different compartments (as evidenced by ci or en expression) can fuse together to form composite clones which have roundish, smooth shapes. Within such composite clones, the characteristic segregation of 'anterior' and 'posterior' cells is maintained - they do not intermix.

    Expression of EgfrDN.Scer\UAS.cBa under the control of Scer\GAL4ap-md544 partially suppresses the extra bristle phenotype seen in ed1X5/edslH8 flies. The number of macrochaetae/heminotum in animals expressing EgfrDN.Scer\UAS.cBa under the control of Scer\GAL4ap-md544 in a ed1X5/edslH8 background is - anterior+posterior notopleural: 2.15, presutural: 1.05, anterior supraalar: 0.80, posterior supraalar: 0, anterior postalar: 0.60, posterior postalar: 0, anterior+posterior dorsocentral: 1.30 and anterior+posterior scutellar: 2.25. N55e11/+ ; ed1X5/edslH8 adults show an almost complete absence of microchaetae on the notum. DlRevF10/+ increases the number of extra macrochaetae on the notum seen in ed1X5/edslH8 adults. H2/+ almost completely eliminates the extra macrochaetae seen on the notum of ed1X5/edslH8 animals, although the increased density of microchaetae seen in ed1X5/edslH8 flies is not reduced by H2/+. The low density of microchaetae on the notum seen in NMcd1/+ flies is not rescued by ed1X5/edslH8.

    ed1X5 suppresses the rough eye phenotype caused by overexpression of argos.

    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Not rescued by
    Comments

    Expression of edΔC.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4tub.PU is unable to rescue the multiple cell layer phenotype seen in ed1X5 mutant follicle cell clones.

    Expression of edTMC.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4tub.PU rescues the multiple cell layer phenotype seen in ed1X5 mutant follicle cell clones.

    Ubiquitous expression of edScer\UAS.cBa under the control of Scer\GAL4C-765 rescues the cell sorting phenotypes seen in ed1X5 mutant wing disc clones. The boundary between the clone and the surrounding wild type cells is no longer smooth edged, instead appearing jagged as in wild type. Normal levels of shg are seen.

    Images (0)
    Mutant
    Wild-type
    Stocks (0)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (2)
    References (10)