G24997064A
G14374A
R361H | zip-PA; R316H | zip-PB; R276H | zip-PC; R321H | zip-PD; R276H | zip-PE; R284H | zip-PF; R276H | zip-PG; R284H | zip-PH; R329H | zip-PI
R276H
Amino acid replacement: R277H.
Nucleotide substitution: G14374A. Amino acid replacement: R276H. Nucleotide coordinates are relative to the sequence record U35816. Amino acid coordinates are relative to the transcript published in FBrf0052857.
zipEbr/zip02957 transheterozygotes are viable but have malformed wings with varying degrees of severity. Mutants also have rough eyes. In these retina the Mysoin-II system is disorganised . Some rhabdomeres have gaps along their lengths. Moreover, the rhabdomeres are not as straight as in wild-type, but appear to meander slightly. At the retinal floor of mutants, the hexagonal arrangement of the pigment cell pedicels exhibit irregularities, moreover, the retinal floor is not completely flattened but wrinkled in some areas.
Mutants die as late embryos or early larvae.
Lethality acts post-embryonically.
zip2/zipEbr has increased mortality during development phenotype, enhanceable by cnomis1/cno2
zip2/zipEbr has increased mortality during development phenotype, enhanceable by PDZ-GEFk13720/PDZ-GEFC2
RhoGEF204291, zipEbr has visible | dominant phenotype, suppressible by Mbs3
zip2/zipEbr is an enhancer of increased mortality during development phenotype of cnomis1/cno2
zip2/zipEbr is an enhancer of abnormal cell migration | embryonic stage phenotype of cnomis1/cno2
zip2/zipEbr is an enhancer of increased mortality during development phenotype of PDZ-GEFk13720/PDZ-GEFC2
zipEbr is a suppressor of lethal | recessive | larval stage phenotype of MoeG0323
zip[+]/zipEbr is a suppressor of lethal | embryonic stage phenotype of Scer\GAL4arm.PS, RokUAS.cMa
zip[+], zipEbr, Scer\GAL4[-] is a suppressor of lethal | maternal effect | embryonic stage phenotype of MbsEP3727/Df(3L)th117, Mbs3
zip[+], zipEbr, Scer\GAL4[-] is a suppressor of lethal | maternal effect | embryonic stage phenotype of Mbs3/Df(3L)th117, MbsEP3727
Scer\GAL4dpp.PU, fweUASp.Lose-A.C.Tag:HA, zip2/zipEbr has increased cell death phenotype
PDZ-GEFk13720/PDZ-GEFC2, zip2/zipEbr has abnormal cell migration | embryonic stage phenotype
cnomis1/cno2, zipEbr has visible | adult stage phenotype
RhoGEF24.1, zip[+]/zipEbr has visible | dominant phenotype
E(zip)18-518-5, zip[+]/zipEbr has visible | dominant phenotype
RhoGEF204291/RhoGEF2[+], zipEbr has visible phenotype
RhoGEF211-3, zip[+]/zipEbr has visible | dominant phenotype
RhoGEF21.1, zip[+]/zipEbr has visible | dominant phenotype
Rho112-6, zip[+]/zipEbr has partially lethal phenotype
E(zip)12-512-5, zip[+]/zipEbr has visible | dominant phenotype
E(zip)12-5[+]/E(zip)12-512-5, zipEbr has visible phenotype
E(zip)18-5[+]/E(zip)18-518-5, zipEbr has visible phenotype
E(zip)31-631-6/E(zip)31-6[+], zipEbr has visible phenotype
RhoGEF21.1/RhoGEF2[+], zipEbr has visible phenotype
RhoGEF211-3/RhoGEF2[+], zipEbr has visible phenotype
RhoGEF24.1/RhoGEF2[+], zipEbr has visible phenotype
E(zip)31-631-6, zip[+]/zipEbr has visible | dominant phenotype
RhoGEF204291, zip[+]/zipEbr has visible | dominant phenotype
E(br)444Ebr444, zip[+]/zipEbr has visible | dominant phenotype
E(br)65Ebr65, zip[+]/zipEbr has visible | dominant phenotype
E(br)121Ebr121/E(br)121[+], zipEbr has visible phenotype
E(br)160[+]/E(br)160Ebr160, zipEbr has visible phenotype
E(br)444[+]/E(br)444Ebr444, zipEbr has visible phenotype
E(br)65Ebr65/E(br)65[+], zipEbr has visible phenotype
Rho1Ebr233/Rho1[+], zipEbr has visible phenotype
Rho1[+]/Rho1Ebr246, zipEbr has visible phenotype
Rho1Ebr233, zip[+]/zipEbr has visible | dominant phenotype
E(br)121Ebr121, zip[+]/zipEbr has visible | dominant phenotype
Rho1Ebr246, zip[+]/zipEbr has visible | dominant phenotype
E(br)160Ebr160, zip[+]/zipEbr has visible | dominant phenotype
RhoGEF204291, zipEbr has visible | dominant phenotype
RhoGEF204291/RhoGEF2[+], zipEbr has visible | dominant phenotype
Rho1J3.8, zip[+]/zipEbr has partially lethal - majority die | dominant phenotype
Rho1E3.10, zipEbr has partially lethal - majority die | dominant phenotype
Rho1J3.8, zipEbr has partially lethal - majority die | dominant phenotype
RhoGEF21.1, zipEbr has visible | dominant phenotype
RhoGEF24.1, zipEbr has visible | dominant phenotype
Rho1E3.10, zip[+]/zipEbr has partially lethal - majority die | dominant phenotype
Df(1)v-L15/+, zipEbr has visible | dominant phenotype
Df(1)v-N48/+, zipEbr has visible | dominant phenotype
Df(2L)30A-C/+, zipEbr has visible | dominant phenotype
Df(2L)TW50/+, zipEbr has visible | dominant phenotype
+/In(2R)bwVDe2LCyR, zipEbr has visible | dominant phenotype
Df(2R)nap1/+, zipEbr has visible | dominant phenotype
In(2LR)Px4/+, zipEbr has visible | dominant phenotype
Df(2L)cl-h3/+, zipEbr has visible | dominant phenotype
Df(2R)bwVDe2LPxKR/+, zipEbr has visible | dominant phenotype
Df(3L)81k19/+, zipEbr has visible | dominant phenotype
Df(3L)81k19/+, zipEbr has partially lethal - majority die | dominant phenotype
Df(3L)st-b11/+, zipEbr has visible | dominant phenotype
Rho1E3.10/Rho1[+], zipEbr has partially lethal - majority die | dominant phenotype
Rho1J3.8/Rho1[+], zipEbr has partially lethal - majority die | dominant phenotype
Df(2R)Jp8, zipEbr has partially lethal - majority die | dominant phenotype
Df(1)64c18/+, zipEbr has visible | dominant phenotype
Rho1J3.8, zip[+]/zipEbr has partially lethal - majority live phenotype
+/Df(1)ct-J4, zipEbr has visible | dominant phenotype
Df(1)ct4b1/+, zipEbr has visible | dominant phenotype
Rho1E3.10, zip[+]/zipEbr has partially lethal - majority live phenotype
Df(1)HF396/+, zipEbr has visible | dominant phenotype
Df(2L)JS32/+, zipEbr has visible | dominant phenotype
Df(2L)sc19-5/+, zipEbr has visible | dominant phenotype
Df(2L)TE29Aa-11/+, zipEbr has visible | dominant phenotype
Df(2L)osp29/+, zipEbr has visible | dominant phenotype
+/Df(2R)M41A4, zipEbr has visible | dominant phenotype
Df(2R)vg-C/+, zipEbr has visible | dominant phenotype
Df(2R)or-BR6/+, zipEbr has visible | dominant phenotype
Df(3L)HR370/+, zipEbr has visible | dominant phenotype
Df(3L)HR119/+, zipEbr has visible | dominant phenotype
Df(3L)GN24/+, zipEbr has visible | dominant phenotype
Df(3L)rdgC-co2/+, zipEbr has visible | dominant phenotype
Df(3L)Pc-MK/+, zipEbr has visible | dominant phenotype
Df(1)sc-J4/+, zipEbr has visible | dominant phenotype
zip2/zipEbr has embryo phenotype, enhanceable by PDZ-GEFk13720/PDZ-GEFC2
zipEbr has phenotype, non-enhanceable by Eip74EFDL-1
zipEbr has phenotype, non-enhanceable by Eip74EFneo24
zipEbr has phenotype, non-enhanceable by l(2)52FbD2.33
zipEbr has phenotype, non-enhanceable by l(2)52FcD8.13
zipEbr has phenotype, non-enhanceable by l(2)52FdE3.27
zipEbr has phenotype, non-enhanceable by l(2)52FeE6.17
zip2/zipEbr is an enhancer of embryonic/first instar larval cuticle | embryonic stage phenotype of cnomis1/cno2
zip2/zipEbr is an enhancer of embryo phenotype of PDZ-GEFk13720/PDZ-GEFC2
PDZ-GEFk13720/PDZ-GEFC2, zip2/zipEbr has embryonic/first instar larval cuticle | embryonic stage phenotype
PDZ-GEFk13720/PDZ-GEFC2, zip2/zipEbr has ectoderm | embryonic stage phenotype
E(zip)31-631-6, zip[+]/zipEbr has wing phenotype
RhoGEF24.1, zip[+]/zipEbr has leg phenotype
E(zip)18-518-5, zip[+]/zipEbr has leg phenotype
E(zip)18-518-5, zip[+]/zipEbr has wing phenotype
RhoGEF211-3, zip[+]/zipEbr has leg phenotype
RhoGEF211-3, zip[+]/zipEbr has wing phenotype
RhoGEF21.1, zip[+]/zipEbr has leg phenotype
E(zip)12-512-5, zipEbr has leg phenotype
E(zip)12-512-5, zipEbr has wing phenotype
E(zip)18-518-5, zipEbr has leg phenotype
E(zip)18-518-5, zipEbr has wing phenotype
E(zip)31-631-6, zipEbr has leg phenotype
E(zip)31-631-6, zipEbr has wing phenotype
RhoGEF211-3, zipEbr has leg phenotype
RhoGEF211-3, zipEbr has wing phenotype
E(zip)12-512-5, zip[+]/zipEbr has leg phenotype
E(zip)12-512-5, zip[+]/zipEbr has wing phenotype
E(zip)31-631-6, zip[+]/zipEbr has leg phenotype
RhoGEF204291, zipEbr has leg phenotype
RhoGEF21.1, zipEbr has leg phenotype
RhoGEF24.1, zipEbr has leg phenotype
RhoGEF204291, zip[+]/zipEbr has leg phenotype
E(br)65Ebr65, zip[+]/zipEbr has leg phenotype
E(br)444Ebr444, zip[+]/zipEbr has leg phenotype
E(br)121Ebr121, zipEbr has leg phenotype
E(br)160Ebr160, zipEbr has leg phenotype
E(br)444Ebr444, zipEbr has leg phenotype
E(br)65Ebr65, zipEbr has leg phenotype
Rho1Ebr233, zipEbr has leg phenotype
Rho1Ebr246, zipEbr has leg phenotype
Rho1Ebr233, zip[+]/zipEbr has leg phenotype
E(br)121Ebr121, zip[+]/zipEbr has leg phenotype
Rho1Ebr246, zip[+]/zipEbr has leg phenotype
E(br)160Ebr160, zip[+]/zipEbr has leg phenotype
RhoGEF204291/RhoGEF2[+], zipEbr has wing phenotype
RhoGEF204291, zipEbr has wing phenotype
RhoGEF24.1, zip[+]/zipEbr has femur phenotype
RhoGEF24.1, zip[+]/zipEbr has tibia phenotype
RhoGEF24.1, zip[+]/zipEbr has wing phenotype
RhoGEF24.1, zipEbr has femur phenotype
RhoGEF24.1, zipEbr has tibia phenotype
RhoGEF24.1, zipEbr has wing phenotype
Df(1)v-L15/+, zipEbr has femur phenotype
Df(1)v-L15/+, zipEbr has tibia phenotype
Df(1)v-N48/+, zipEbr has femur phenotype
Df(1)v-N48/+, zipEbr has tibia phenotype
Df(2L)30A-C/+, zipEbr has femur phenotype
Df(2L)30A-C/+, zipEbr has tibia phenotype
Df(2L)TW50/+, zipEbr has femur phenotype
Df(2L)TW50/+, zipEbr has tibia phenotype
+/In(2R)bwVDe2LCyR, zipEbr has femur phenotype
+/In(2R)bwVDe2LCyR, zipEbr has tibia phenotype
Df(2R)nap1/+, zipEbr has femur phenotype
Df(2R)nap1/+, zipEbr has tibia phenotype
In(2LR)Px4/+, zipEbr has femur phenotype
In(2LR)Px4/+, zipEbr has tibia phenotype
Df(2L)cl-h3/+, zipEbr has femur phenotype
Df(2L)cl-h3/+, zipEbr has tibia phenotype
Df(2R)bwVDe2LPxKR/+, zipEbr has femur phenotype
Df(2R)bwVDe2LPxKR/+, zipEbr has tibia phenotype
Df(3L)81k19/+, zipEbr has femur phenotype
Df(3L)81k19/+, zipEbr has tibia phenotype
Df(3L)st-b11/+, zipEbr has femur phenotype
Df(3L)st-b11/+, zipEbr has tibia phenotype
Df(1)64c18/+, zipEbr has femur phenotype
Df(1)64c18/+, zipEbr has tibia phenotype
+/Df(1)ct-J4, zipEbr has femur phenotype
+/Df(1)ct-J4, zipEbr has tibia phenotype
Df(1)ct4b1/+, zipEbr has femur phenotype
Df(1)ct4b1/+, zipEbr has tibia phenotype
Df(1)HF396/+, zipEbr has femur phenotype
Df(1)HF396/+, zipEbr has tibia phenotype
Df(2L)JS32/+, zipEbr has femur phenotype
Df(2L)JS32/+, zipEbr has tibia phenotype
Df(2L)sc19-5/+, zipEbr has femur phenotype
Df(2L)sc19-5/+, zipEbr has tibia phenotype
Df(2L)TE29Aa-11/+, zipEbr has femur phenotype
Df(2L)TE29Aa-11/+, zipEbr has tibia phenotype
Df(2L)osp29/+, zipEbr has femur phenotype
Df(2L)osp29/+, zipEbr has tibia phenotype
+/Df(2R)M41A4, zipEbr has femur phenotype
+/Df(2R)M41A4, zipEbr has tibia phenotype
Df(2R)vg-C/+, zipEbr has femur phenotype
Df(2R)vg-C/+, zipEbr has tibia phenotype
Df(2R)or-BR6/+, zipEbr has femur phenotype
Df(2R)or-BR6/+, zipEbr has tibia phenotype
Df(3L)HR370/+, zipEbr has femur phenotype
Df(3L)HR370/+, zipEbr has tibia phenotype
Df(3L)HR119/+, zipEbr has femur phenotype
Df(3L)HR119/+, zipEbr has tibia phenotype
Df(3L)GN24/+, zipEbr has femur phenotype
Df(3L)GN24/+, zipEbr has tibia phenotype
Df(3L)rdgC-co2/+, zipEbr has femur phenotype
Df(3L)rdgC-co2/+, zipEbr has tibia phenotype
Df(3L)Pc-MK/+, zipEbr has femur phenotype
Df(3L)Pc-MK/+, zipEbr has tibia phenotype
Df(1)sc-J4/+, zipEbr has femur phenotype
Df(1)sc-J4/+, zipEbr has tibia phenotype
A zip2/zipEbr mutant background (which reducing tension in cells) induces cell death at the anterior boundary when fweScer\UAS.P\T.Lose-A.C.T:Ivir\HA1 is expressed under the control of Scer\GAL4dpp.PU in the anterior compartment of the wing disc.
The frequency of the malformed leg phenotype seen in Sb63b/+ heterozygotes (8%) is increased if the flies are also heterozygous for zipEbr (63%). The frequency of the malformed leg phenotype seen in Sb70/+ heterozygotes (7%) is increased if the flies are also heterozygous for zipEbr (96%). The frequency of the malformed leg phenotype seen in br1/Y males (1%) is increased if the flies are also heterozygous for zipEbr (56%).
zipEbr shows no interaction with sqhunspecified (assayed in terms of a malformed leg phenotype). zipEbr shows a strong interaction (at least 50% of double heterozygotes have at least one malformed leg) with the following mutations: RhoGEF211-3, E(zip)12-512-5 and Rho112-6. zipEbr shows a moderate interaction (25-49% of double heterozygotes have at least one malformed leg) with the following mutations: E(zip)18-518-5. zipEbr shows a weak interaction (5-24% of double heterozygotes have at least one malformed leg) with the following mutations: E(zip)31-631-6. zipEbr shows a strong interaction (at least 50% of double heterozygotes have at least one malformed wing) with the following mutations: rok1, Sb70, RhoGEF211-3, E(zip)12-512-5, Rho112-6 and E(zip)31-631-6. zipEbr shows a moderate interaction (25-49% of double heterozygotes have at least one malformed wing) with the following mutations: E(zip)18-518-5.
The fraction of flies showing a malformed leg phenotype in at least one leg, for zipEbr in double heterozygous combination with one of the following alleles is - SbEbr20 : 46%, SbEbr48 : 30%, SbEbr228 : 14%, SbEbr448 : 57%, SbEbr536 : 33%, SbEbr623 : 26%, Rho1Ebr233 : 93%, Rho1Ebr246 : 83%, bsEbr292 : 2%, E(br)24Ebr24 : 9%, E(br)65Ebr65 : 67%, E(br)155Ebr155 : 7%, E(br)165Ebr165 : 6%, E(br)333Ebr333 : 4%, E(br)72Ebr72 : 4%, E(br)121Ebr121 : 82%, E(br)160Ebr160 : 20%, E(br)187Ebr187 : 6%, E(br)420Ebr420 : 15% and E(br)444Ebr444 : 84%.
The lethality of Mbs mutant embryos derived from MbsEP3727/Df(3L)th117 females mated to Mbs3/+ males is suppressed by zipEbr/+.
27% of RhoGEF24.1/zipEbr double heterozygotes have a malformed leg phenotype, which shows variable expressivity. In less severe cases, there is a dent in the femur or tibia. Flies with malformed legs often also show wing defects. Weak phenotypes include a broadening of the wing blade and folding back of the distal portion of the wing blade. In the most severe cases, wings are greatly reduced in size. 38% of RhoGEF204291/zipEbr double heterozygotes have a malformed leg phenotype. 33% of RhoGEF21.1/zipEbr double heterozygotes have a malformed leg phenotype. 100% of Rho1J3.8/zipEbr double heterozygotes have a malformed leg phenotype. 100% of Rho1E3.10/zipEbr double heterozygotes have a malformed leg phenotype, which shows variable expressivity. In more severe cases, a dent and twisting of the femur is apparent, along with shortening and thickening of the tibia. Notching of the posterior region of the wing blade is also seen. 96% of Rho172F/zipEbr double heterozygotes have a malformed leg phenotype. Wing defects are also seen. Weak phenotypes include a broadening of the wing blade and folding back of the distal portion of the wing blade. In more severe cases, wings lacking adhesion between the wing blade bilayer and any apparent wing venation are seen. 92% of Rho172O/zipEbr double heterozygotes have a malformed leg phenotype. 90% of Df(2R)Jp4/zipEbr double heterozygotes have a malformed leg phenotype. 98% of Df(2R)Jp8/zipEbr double heterozygotes have a malformed leg phenotype.
Produces malformed phenotype when in double heterozygosis with second site zip non-complementing mutants. Shows a higher level of penetrance with second site non-complementers than does zipmhc-c6.1.