dorsal mesothoracic disc & peripodial epithelium | somatic clone, with Scer\GAL4αTub84B.PL
dorsal mesothoracic disc & peripodial epithelium | somatic clone | cell non-autonomous, with Scer\GAL4αTub84B.PL
sensory mother cell & dorsal mesothoracic disc | ectopic, with Scer\GAL4sd-SG29.1
The co-expression of wgUAS.cGa and pnutGD1512 from the second instar larval stage under the combined control of Scer\GAL4ap-md544 and Gal80[ts] does not leads to the formation of tumors in the third instar larval wing disc.
Marker expression data suggests that the presence of vgScer\UAS.cZa transforms wgScer\UAS.cGa; Scer\GAL4αTub84B.PL somatic clone cells in the peripodial epithelium from wing hinge like to wing pouch like.
Co-expression of wgScer\UAS.cGa and Ras85DG13Q.Scer\UAS under the control of Scer\GAL4twi.PG results in a lateral expansion of the eve-expressing progenitor clusters in the mesoderm. Co-expression of twiScer\UAS.cBa, Ras85DG13Q.Scer\UAS and wgScer\UAS.cGa under the control of Scer\GAL4twi.PG results in an almost continuous anteroposterior stripe of eve-expressing progenitor cells confined to the dorsal mesoderm. The number of extra eve-expressing progenitor cells in the mesoderm is substantially increased when twiScer\UAS.cBa is co-expressed with wgScer\UAS.cGa under the control of Scer\GAL4twi.PG.
The addition of wgScer\UAS.cGa suppresses the wing pouch phenotype seen in NECN.Scer\UAS/Scer\GAL4sd-SG29.1 wing discs and leads to cell death occurring over the whole of the wing pouch. Wings in these adult flies are small and bear a small number of bristles. The addition of wgScer\UAS.cGa to NECN.Scer\UAS/Scer\GAL4sd-SG29.1 flies leads to an increase in the area of the wing disc covered by SOPs, similar to when wgScer\UAS.cGa is expressed alone, though they are not evenly spaced.
Coexpression of dppScer\UAS.cSa and wgScer\UAS.cGa under the control of Scer\GAL4vg.int2.1 leads to a pronounced extension of the wing blade towards the notum in the wing disc. In some cases, the development of up to 8 wing blades, all with a common origin over a point ventral to the notum, is seen.
Expression of wgScer\UAS.cGa under the control of Scer\GAL4pros.PMG partially rescues the loss of naked cuticle in wgl-17 larvae, but reduces denticle diversity. This expression restores RP2 neurons to wgl-17 embryos.
wgScer\UAS.cGa; Scer\GAL4da.G32 rescues the loss of naked ventral cuticle in wgl-17 homozygous embryos. About half of the resulting embryos have the wgScer\UAS.cGa; Scer\GAL4da.G32 overexpression phenotype of a completely naked ventral cuticle. wgScer\UAS.cGa; Scer\GAL4da.G32 rescues the dorsal-ventral elongation and orientation of microtubule bundles in the leading edge cells of stage 13 wgl-17 homozygous embryos. wgScer\UAS.cGa; Scer\GAL4332.3 rescues the dorsal-ventral polarity of leading edge cells in these embryos, including orientation of the microtubule bundles. But dorsal-ventral elongation of these cells is occurs later than normal and is reduced.