FlyBase Allele Report: Dmel\wg[UAS.cGa]

General Information

Symbol

Dmel\wg^UAS.cGa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Gieseler

FlyBase ID

FBal0088531

Feature type

allele

Associated gene

Dmel\wg

Associated Insertion(s)

Carried in Construct

P{UAS-wg.G}

Also Known As

UAS-wg, UAS-wg^E, UASwg^E1, UASwg

Key Links

Allele class

Mutagen

in vitro construct - regulatory fusion

Nature of the Allele

Allele class

Mutagen

in vitro construct - regulatory fusion

(Klein and Martinez Arias, 1998)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{UAS-wg.G}

Cytology

Nature of the lesion

Statement

Reference

UAS regulatory sequences drive expression of wg.

(Klein and Martinez Arias, 1998)

Allele components

Product class / Tool use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^sd-SG29.1

(Brennan et al., 1999)

Phenotype Manifest In

abdominal ventral acute muscle 1 founder cell | increased number, with Scer\GAL4^Mef2.PR

(Cox and Baylies, 2005)

abdominal ventral acute muscle 1 founder cell | increased number, with Scer\GAL4^twi.PB

(Cox and Baylies, 2005)

abdominal ventral acute muscle 2 founder cell | increased number, with Scer\GAL4^Mef2.PR

(Cox and Baylies, 2005)

abdominal ventral acute muscle 2 founder cell | increased number, with Scer\GAL4^twi.PB

(Cox and Baylies, 2005)

abdominal ventral acute muscle 3 founder cell | increased number, with Scer\GAL4^Mef2.PR

(Cox and Baylies, 2005)

abdominal ventral acute muscle 3 founder cell | increased number, with Scer\GAL4^twi.PB

(Cox and Baylies, 2005)

dorsal mesothoracic disc & peripodial epithelium | somatic clone, with Scer\GAL4^αTub84B.PL

(Baena-Lopez et al., 2003)

dorsal mesothoracic disc & peripodial epithelium | somatic clone | cell non-autonomous, with Scer\GAL4^αTub84B.PL

(Baena-Lopez et al., 2003)

macrochaeta, with Scer\GAL4^dpp.blk1

(Whitworth and Russell, 2003)

scutellum, with Scer\GAL4^dpp.blk1

(Whitworth and Russell, 2003)

sensory mother cell & dorsal mesothoracic disc | ectopic, with Scer\GAL4^sd-SG29.1

(Brennan et al., 1999)

wing, with Scer\GAL4^sd-SG29.1

(Brennan et al., 1999)

wing | ectopic | proximal, with Scer\GAL4^dpp.blk1

(Whitworth and Russell, 2003)

wing | proximal, with Scer\GAL4^zfh2-MS209

(Russell, 2000)

wing blade anterior compartment, with Scer\GAL4^sd-SG29.1

(Brennan et al., 1999)

wing disc, with Scer\GAL4^dpp.blk1

(Brennan et al., 1999, Klein and Martinez Arias, 1999)

wing disc, with Scer\GAL4^sd-SG29.1

(Brennan et al., 1999)

wing disc, with Scer\GAL4^zfh2-MS209

(Russell, 2000)

wing hinge | precursor, with Scer\GAL4^sd-SG29.1

(Brennan et al., 1999)

wing hinge primordium | ectopic | larval stage | somatic clone, with Scer\GAL4^αTub84B.PL

(Baena-Lopez et al., 2003)

wing margin bristle | ectopic, with Scer\GAL4^sd-SG29.1

(Brennan et al., 1999)

wing sclerite | ectopic, with Scer\GAL4^dpp.blk1

(Whitworth and Russell, 2003)

Detailed Description

Statement

Reference

When wg^Scer\UAS.cGa is driven by Scer\GAL4^twi.PB or Scer\GAL4^Mef2.PR the cII cluster of slou expressing muscle founder cells is expanded.

(Cox and Baylies, 2005)

Expression of wg^Scer\UAS.cGa in ganglion mother cells, driven by Scer\GAL4^pros.PMG, does not affect slou-expressing founder cell clusters I and II.

(Cox et al., 2005)

wg^Scer\UAS.cGa; Scer\GAL4^αTub84B.PL somatic clones in the peripodial epithelium of the wing disc are transformed from squamous to columnar morphology. This transformation occurs not just within the clone but affects cells non-autonomously within a range of 7-15 cellular diameters away from the clone. The severity of the morphological change decreases with distance to the clone Marker expression suggests that these clones are transformed towards a wing hinge fate, rather than wing blade or notum.

(Baena-Lopez et al., 2003)

In wg^Scer\UAS.cGa, Scer\GAL4^dpp.blk1 pharate adults a loss of macrochaete and scutellum is seen as well as ectopic outgrowth and sclerites characteristic of proximal wing structures.

(Whitworth and Russell, 2003)

Expression of wg^Scer\UAS.cGa under the control of Scer\GAL4^twi.PG has no stable effect on eve-expressing progenitor formation in the mesoderm.

(Halfon et al., 2000)

Ectopic expression of wg^Scer\UAS.cGa driven by Scer\GAL4^zfh2-MS209 leads to an overgrown wing disc and subsequently an overgrowth in the proximal wing region.

(Russell, 2000)

The three slou-expressing muscles are largely normal in embryos expressing wg^Scer\UAS.cGa under the control of Scer\GAL4^twi.PB.

(Brennan et al., 1999)

Overexpression of wg^Scer\UAS.cGa driven by Scer\GAL4^sd-SG29.1 increases the size of the hinge region of the wing disc but does not effect the viability of the cells. The adult wing is smaller than wild-type with a large number of sensory organs. When expression of wg^Scer\UAS.cGa is driven by Scer\GAL4^sd-SG29.1, the whole of the anterior compartment of the wing disc is covered in evenly spaced SOPs (sensory organ precursors). Expression of wg^Scer\UAS.cGa during the induction of the wing primordia in the early phases of wing development by Scer\GAL4^dpp.blk1, leads to an increased wing primordia which distorts the disc as the primordia invades the notum.

(Brennan et al., 1999)

Flies expressing wg^Scer\UAS.cGa under the control of Scer\GAL4^dpp.blk1 show morphological changes in the wing disc, due to ectopic induction of hinge fate in the notal area. Expression of wg^Scer\UAS.cGa under the control of Scer\GAL4^vg.int2.1 does not enlarge the wing pouch.

(Klein and Martinez Arias, 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

wg^UAS.cGa, Scer\GAL4^sd-SG29.1 is a suppressor of visible phenotype of N^ECN.UAS, Scer\GAL4^sd-SG29.1

(Brennan et al., 1999)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Scer\GAL4^zfh2-MS209, wg^UAS.cGa has wing disc phenotype, non-enhanceable by Scer\GAL4^zfh2-MS209

(Russell, 2000)

Scer\GAL4^zfh2-MS209, wg^UAS.cGa has wing | proximal phenotype, non-enhanceable by Scer\GAL4^zfh2-MS209

(Russell, 2000)

Suppressed by

Statement

Reference

Scer\GAL4^Mef2.PR, wg^UAS.cGa has abdominal ventral acute muscle 1 founder cell | increased number phenotype, suppressible by hh^AC/hh⁸

(Cox and Baylies, 2005)

Scer\GAL4^Mef2.PR, wg^UAS.cGa has abdominal ventral acute muscle 2 founder cell | increased number phenotype, suppressible by hh^AC/hh⁸

(Cox and Baylies, 2005)

Scer\GAL4^Mef2.PR, wg^UAS.cGa has abdominal ventral acute muscle 3 founder cell | increased number phenotype, suppressible by hh^AC/hh⁸

(Cox and Baylies, 2005)

NOT suppressed by

Statement

Reference

Scer\GAL4^zfh2-MS209, wg^UAS.cGa has wing disc phenotype, non-suppressible by Scer\GAL4^zfh2-MS209

(Russell, 2000)

Scer\GAL4^zfh2-MS209, wg^UAS.cGa has wing | proximal phenotype, non-suppressible by Scer\GAL4^zfh2-MS209

(Russell, 2000)

Enhancer of

Statement

Reference

wg^UAS.cGa/twi^UAS.cBa, Scer\GAL4^twi.PG is an enhancer of mesoderm phenotype of Ras85D^G13Q.UAS, Scer\GAL4^twi.PG

(Halfon et al., 2000)

wg^UAS.cGa/Ras85D^G13Q.UAS, Scer\GAL4^twi.PG is an enhancer of mesoderm phenotype of Scer\GAL4^twi.PG, twi^UAS.cBa

(Halfon et al., 2000)

wg^UAS.cGa, Scer\GAL4^twi.PG is an enhancer of mesoderm phenotype of Ras85D^G13Q.UAS, Scer\GAL4^twi.PG

(Halfon et al., 2000)

Scer\GAL4^twi.PG/wg^UAS.cGa is an enhancer of mesoderm phenotype of Scer\GAL4^twi.PG, twi^UAS.cBa

(Halfon et al., 2000)

Suppressor of

Statement

Reference

wg^UAS.cGa/Scer\GAL4^69B is a suppressor | partially of embryonic/first instar larval cuticle | dorsal phenotype of hep¹

(Kaltschmidt et al., 2002)

wg^UAS.cGa/Scer\GAL4^69B is a suppressor | partially of amnioserosa phenotype of hep¹

(Kaltschmidt et al., 2002)

wg^UAS.cGa/Scer\GAL4^69B is a suppressor | partially of embryonic dorsal epidermis phenotype of hep¹

(Kaltschmidt et al., 2002)

wg^UAS.cGa/Scer\GAL4^69B is a suppressor | partially of embryonic leading edge cell & actin filament phenotype of hep¹

(Kaltschmidt et al., 2002)

wg^UAS.cGa, Scer\GAL4^sd-SG29.1 is a suppressor of wing disc phenotype of N^ECN.UAS, Scer\GAL4^sd-SG29.1

(Brennan et al., 1999)

wg^UAS.cGa, Scer\GAL4^sd-SG29.1 is a suppressor of wing phenotype of N^ECN.UAS, Scer\GAL4^sd-SG29.1

(Brennan et al., 1999)

NOT Suppressor of

Statement

Reference

wg^UAS.cGa/Scer\GAL4^dpp.blk1 is a non-suppressor of wing phenotype of Su(H)⁸/Su(H)²

(Klein and Martinez Arias, 1998)

wg^UAS.cGa/Scer\GAL4^dpp.blk1 is a non-suppressor of wing phenotype of vg^83b27-R

(Klein and Martinez Arias, 1998)

wg^UAS.cGa/Scer\GAL4^dpp.blk1 is a non-suppressor of wing phenotype of Ser^CS94

(Klein and Martinez Arias, 1998)

wg^UAS.cGa/Scer\GAL4^dpp.blk1 is a non-suppressor of wing phenotype of ap^UGO35

(Klein and Martinez Arias, 1998)

Other

Statement

Reference

Scer\GAL4^αTub84B.PL, vg^UAS.cZa, wg^UAS.cGa has wing pouch | ectopic | somatic clone | larval stage phenotype

(Baena-Lopez et al., 2003)

N^{ECNΔ17-19.24-26.UAS}, Scer\GAL4^sd-SG29.1, wg^UAS.cGa has sensory mother cell & dorsal mesothoracic disc | ectopic phenotype

(Brennan et al., 1999)

N^{ECNΔ10-12.UAS}, Scer\GAL4^sd-SG29.1, wg^UAS.cGa has sensory mother cell & dorsal mesothoracic disc | ectopic phenotype

(Brennan et al., 1999)

Scer\GAL4^vg.int2.1, dpp^UAS.cSa, wg^UAS.cGa has wing disc phenotype

(Klein and Martinez Arias, 1999)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of wg^UAS.cGa and pnut^GD1512 from the second instar larval stage under the combined control of Scer\GAL4^ap-md544 and Gal80[ts] does not leads to the formation of tumors in the third instar larval wing disc.

(Gerlach et al., 2018)

Marker expression data suggests that the presence of vg^Scer\UAS.cZa transforms wg^Scer\UAS.cGa; Scer\GAL4^αTub84B.PL somatic clone cells in the peripodial epithelium from wing hinge like to wing pouch like.

(Baena-Lopez et al., 2003)

Co-expression of wg^Scer\UAS.cGa and Ras85D^{G13Q.Scer\UAS} under the control of Scer\GAL4^twi.PG results in a lateral expansion of the eve-expressing progenitor clusters in the mesoderm. Co-expression of twi^Scer\UAS.cBa, Ras85D^{G13Q.Scer\UAS} and wg^Scer\UAS.cGa under the control of Scer\GAL4^twi.PG results in an almost continuous anteroposterior stripe of eve-expressing progenitor cells confined to the dorsal mesoderm. The number of extra eve-expressing progenitor cells in the mesoderm is substantially increased when twi^Scer\UAS.cBa is co-expressed with wg^Scer\UAS.cGa under the control of Scer\GAL4^twi.PG.

(Halfon et al., 2000)

The addition of D¹⁰ to flies with wg^Scer\UAS.cGa driven by Scer\GAL4^zfh2-MS209 has no effect on the phenotype seen in wg^Scer\UAS.cGa,Scer\GAL4^zfh2-MS209 flies alone.

(Russell, 2000)

The addition of wg^Scer\UAS.cGa suppresses the wing pouch phenotype seen in N^ECN.Scer\UAS/Scer\GAL4^sd-SG29.1 wing discs and leads to cell death occurring over the whole of the wing pouch. Wings in these adult flies are small and bear a small number of bristles. The addition of wg^Scer\UAS.cGa to N^ECN.Scer\UAS/Scer\GAL4^sd-SG29.1 flies leads to an increase in the area of the wing disc covered by SOPs, similar to when wg^Scer\UAS.cGa is expressed alone, though they are not evenly spaced.

(Brennan et al., 1999)

Coexpression of dpp^Scer\UAS.cSa and wg^Scer\UAS.cGa under the control of Scer\GAL4^vg.int2.1 leads to a pronounced extension of the wing blade towards the notum in the wing disc. In some cases, the development of up to 8 wing blades, all with a common origin over a point ventral to the notum, is seen.

(Klein and Martinez Arias, 1999)

Scer\GAL4^dpp.blk1-mediated expression in Su(H)²/Su(H)⁸, vg^83b27-R or ap^UGO35 mutants fails to rescue loss of wing tissue.

(Klein and Martinez Arias, 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

wg^UAS.cGa/Scer\GAL4^da.G32 rescues wg^l-17

(Kaltschmidt et al., 2002)

Partially rescues

Scer\GAL4^pros.PMG/wg^UAS.cGa partially rescues wg^l-17

(Cox et al., 2005)

wg^UAS.cGa/Scer\GAL4^332.3 partially rescues wg^l-17

(Morel and Arias, 2004)

wg^UAS.cGa/Scer\GAL4^da.G32 partially rescues wg^l-17

(Morel and Arias, 2004)

Comments

Expression of wg^Scer\UAS.cGa under the control of Scer\GAL4^pros.PMG partially rescues the loss of naked cuticle in wg^l-17 larvae, but reduces denticle diversity. This expression restores RP2 neurons to wg^l-17 embryos.

(Cox et al., 2005)

wg^Scer\UAS.cGa; Scer\GAL4^da.G32 rescues the loss of naked ventral cuticle in wg^l-17 homozygous embryos. About half of the resulting embryos have the wg^Scer\UAS.cGa; Scer\GAL4^da.G32 overexpression phenotype of a completely naked ventral cuticle. wg^Scer\UAS.cGa; Scer\GAL4^da.G32 rescues the dorsal-ventral elongation and orientation of microtubule bundles in the leading edge cells of stage 13 wg^l-17 homozygous embryos. wg^Scer\UAS.cGa; Scer\GAL4^332.3 rescues the dorsal-ventral polarity of leading edge cells in these embryos, including orientation of the microtubule bundles. But dorsal-ventral elongation of these cells is occurs later than normal and is reduced.

(Morel and Arias, 2004)

wg^Scer\UAS.cGa; Scer\GAL4^da.G32 s the polarity and organization of leading edge cells during dorsal closure in wg^l-17 mutant embryos.

(Kaltschmidt et al., 2002)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

wg^Scer\UAS.cGa

wg^UAS.cGa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Gieseler

(Klein and Martinez Arias, 1998)

Secondary FlyBase IDs

References (15)

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