FlyBase Allele Report: Dmel\Rho1[k02107b]

(Bertet et al., 2009, Rothenfluh et al., 2006, Sepp and Auld, 2003, Ward et al., 2003, Magie et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body size | P-stage

(Green et al., 2016)

lethal | recessive

(BDGP Project Members, 1994-1999)

lethal - all die before end of P-stage

(Green et al., 2016)

lethal - all die during embryonic stage

(Sepp and Auld, 2003)

lethal - all die during embryonic stage | recessive

(Magie et al., 1999)

some die during embryonic stage

(Sepp and Auld, 2003)

some die during embryonic stage | recessive

(Magie et al., 1999)

Phenotype Manifest In

PNS glial cell

(Sepp and Auld, 2003)

presumptive embryonic salivary gland

(Xu et al., 2008)

presumptive embryonic salivary gland (with Df(2R)Jp1)

(Xu et al., 2008)

pupa

(Green et al., 2016)

Detailed Description

Statement

Reference

Rho1^k02107b homozygotes are lethal, pupae are abnormally thin but third instar larvae present no obvious muscle phenotype, as compared to controls.

(Green et al., 2016)

The majority of salivary glands fail to invaginate in homozygous embryos, with the cells remaining at the ventral surface of the embryo. These invagination defects are first observed in late stage 11. In those mutant embryos where the salivary glands do invaginate, invagination always starts at the correct dorsal-posterior position.

The salivary gland epithelium of homozygous embryos consists of mesenchymal shaped cells with disorganised cortical F-actin (in contrast to control cells, which are columnar in shape and have prominent cortical F-actin).

Rho1^k02107b/Df(2R)Jp1 embryos show defects in salivary gland migration and invagination.

(Xu et al., 2008)

Mutant embryos show a lack of peripheral glial sheaths in the lateral region of the embryo and loss of glia at the CNS/PNS transition zone.

(Sepp and Auld, 2003)

Strong allele.

(Magie et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

GluRIIA^SP16, Rho1[+]/Rho1^k02107b has abnormal neurophysiology | third instar larval stage | recessive phenotype, non-enhanceable by Exn^EY01953/Exn[+]

(Frank et al., 2009)

Enhancer of

Statement

Reference

Rho1[+]/Rho1^k02107b is an enhancer of visible | dominant phenotype of Sb⁷⁰

(Chen et al., 2004)

Rho1[+]/Rho1^k02107b is an enhancer of visible | dominant phenotype of br¹

(Chen et al., 2004)

Rho1[+]/Rho1^k02107b is an enhancer of visible | dominant phenotype of Sb^63b

(Chen et al., 2004)

Rho1[+]/Rho1^k02107b is an enhancer of visible phenotype of br¹

(Ward et al., 2003)

Suppressor of

Statement

Reference

Rho1[+]/Rho1^k02107b is a suppressor of chemical resistant phenotype of RhoGAP18B¹

(Rothenfluh et al., 2006)

Rho1[+]/Rho1^k02107b is a suppressor of visible phenotype of LIMK1^UAS.cCa, Scer\GAL4^en-e16E

(Chen et al., 2004)

Other

Statement

Reference

GluRIIA^SP16, Rho1[+]/Rho1^k02107b has abnormal neurophysiology | recessive | third instar larval stage phenotype

(Frank et al., 2009)

E(br)121^Ebr121, Rho1[+]/Rho1^k02107b has visible | dominant phenotype

(Ward et al., 2003)

E(br)444^Ebr444, Rho1[+]/Rho1^k02107b has visible | dominant phenotype

(Ward et al., 2003)

E(br)121^Ebr121/E(br)121[+], Rho1^k02107b has visible phenotype

(Ward et al., 2003)

E(br)444[+]/E(br)444^Ebr444, Rho1^k02107b has visible phenotype

(Ward et al., 2003)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

GluRIIA^SP16, Rho1[+]/Rho1^k02107b has embryonic/larval neuromuscular junction phenotype, non-enhanceable by Exn^EY01953/Exn[+]

(Frank et al., 2009)

Suppressed by

Statement

Reference

Rho1^k02107b has presumptive embryonic salivary gland phenotype, suppressible by Scer\GAL4^fkh.PH/crb^UAS.cWa

(Xu et al., 2008)

Rho1^k02107b has presumptive embryonic salivary gland phenotype, suppressible by Scer\GAL4^fkh.PH/Rok^CAT.UAS

(Xu et al., 2008)

Enhancer of

Statement

Reference

Rho1[+]/Rho1^k02107b is an enhancer of leg phenotype of Sb⁷⁰

(Chen et al., 2004)

Rho1[+]/Rho1^k02107b is an enhancer of leg phenotype of br¹

(Chen et al., 2004, Ward et al., 2003)

Rho1[+]/Rho1^k02107b is an enhancer of leg phenotype of Sb^63b

(Chen et al., 2004)

Suppressor of

Statement

Reference

Rho1^k02107b is a suppressor of ectoderm | extended germ band stage phenotype of upd1^YM55

(Bertet et al., 2009)

Rho1[+]/Rho1^k02107b is a suppressor of wing phenotype of LIMK1^UAS.cCa, Scer\GAL4^en-e16E

(Chen et al., 2004)

Other

Statement

Reference

GluRIIA^SP16, Rho1[+]/Rho1^k02107b has embryonic/larval neuromuscular junction phenotype

(Frank et al., 2009)

E(br)121^Ebr121, Rho1[+]/Rho1^k02107b has leg phenotype

(Ward et al., 2003)

E(br)444^Ebr444, Rho1[+]/Rho1^k02107b has leg phenotype

(Ward et al., 2003)

E(br)121^Ebr121, Rho1^k02107b has leg phenotype

(Ward et al., 2003)

E(br)444^Ebr444, Rho1^k02107b has leg phenotype

(Ward et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

An average of 6% os^upd-4, Rho1^k02107b homozygous mutant embryos show germ-band extension defects, much reduced compared to os^upd-4 homozygous mutant embryos.

(Bertet et al., 2009)

The Rho1^k02107b/+ heterozygous mutation can partially suppress the GluRIIA^SP16 NMJ synaptic homeostatic response.

The Exn^EY01953/+ heterozygous mutation does not enhance the Rho1^k02107b/+, GluRIIA^SP16 double mutant NMJ synaptic homeostasis phenotype.

(Frank et al., 2009)

Expression of crb^Scer\UAS.cWa under the control of Scer\GAL4^fkh.PH partially suppresses the salivary gland invagination defects of Rho1^k02107b embryos; the fraction of non-invaginated glands decreases from 80% to 68%, and the fraction of partially invaginated glands increases from 18% to 29%.

Expression of rok^CAT.Scer\UAS under the control of Scer\GAL4^fkh.PH partially suppresses the salivary gland invagination defects of Rho1^k02107b embryos.

(Xu et al., 2008)

Heterozygosity for Rho1^k02107b or Df(2R)Jp6 (which removes Rho1) strongly suppresses the RhoGAP18B¹ ethanol-resistance phenotype.

(Rothenfluh et al., 2006)

Rho1^k02107b/+ suppresses the mutant wing phenotype caused by expression of LIMK1^Scer\UAS.cCa under the control of Scer\GAL4^en-e16E (the % of wings with normal morphology at 18^oC is increased from 9% to 95%). The frequency of the malformed leg phenotype seen in Sb^63b/+ heterozygotes (8%) is increased if the flies are also heterozygous for Rho1^k02107b (93%). The frequency of the malformed leg phenotype seen in Sb⁷⁰/+ heterozygotes (7%) is increased if the flies are also heterozygous for Rho1^k02107b (95%). The frequency of the malformed leg phenotype seen in br¹/Y males (1%) is increased if the flies are also heterozygous for Rho1^k02107b (55%).

(Chen et al., 2004)

Dominantly enhances the frequency of br¹ mutant animals with malformed legs. The fraction of flies showing a malformed leg phenotype in at least one leg, for Rho1^k02107b in double heterozygous combination with one of the following alleles is - Sb^Ebr20 : 8%, Sb^Ebr48 : 2%, Sb^Ebr228 : 12%, Sb^Ebr448 : 2%, Sb^Ebr536 : 10%, Sb^Ebr623 : 2%, bs^Ebr292 : 0%, E(br)24^Ebr24 : 3%, E(br)65^Ebr65 : 6%, E(br)155^Ebr155 : 0%, E(br)165^Ebr165 : 7%, E(br)333^Ebr333 : 6%, E(br)72^Ebr72 : 5%, E(br)121^Ebr121 : 56%, E(br)160^Ebr160 : 8%, E(br)187^Ebr187 : 2%, E(br)420^Ebr420 : 2% and E(br)444^Ebr444 : 37%.

(Ward et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Rho1^Ebr233

(Ward et al., 2003)

Rho1^Ebr246

(Ward et al., 2003)

Rho1^k07236

(Magie et al., 1999)

Partially rescued by

Rho1^k02107b is partially rescued by Scer\GAL4^fkh.PH/Rho1^UAS.cHa

(Xu et al., 2008)

Comments

Expression of Rho1^Scer\UAS.cHa under the control of Scer\GAL4^fkh.PH partially rescues the salivary gland defects of Rho1^k02107b embryos; with the fraction of non-invaginated glands decreasing from 80% to 27% and the fraction of completely invaginated glands increasing from 2% to 58%.

(Xu et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

12185

y¹ w^67c23; P{lacW}k02107a P{lacW}Rho1^k02107b/CyO

Notes on Origin

Discoverer

I. Kiss.

(BDGP Project Members, 1994-1999)

Comments

This allele was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was discarded from the gene disruption project prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Spradling (spradling@mail1.ciwemb.edu).

Complements: l(2)05070⁰⁵⁰⁷⁰. Complements: l(2)44DEa⁰⁵⁸⁴⁷. Complements: ptc^k02507. Complements: l(2)44DEa^k05512. Complements: spin^k06110. Complements: Vps25^k08904. Complements: spin^k09905. Complements: l(2)k15617^k15617. Complements: l(2)s1878^s1878.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

Rho1^K02107b

(Xu et al., 2008)

Rho1^K

(Xu et al., 2008)

Rho1^k02107

(Ward et al., 2003)

Rho1^k02107b

(Green et al., 2016, Frank et al., 2009, Rothenfluh et al., 2006)

Rho1^l(2)k02107

(Magie et al., 1999)

RhoAk02107a

(Sepp and Auld, 2003)

l(2)k02107b^k02107b

(BDGP Project Members, 1994-1999)

rho1^K2107B

(Bertet et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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