FlyBase Allele Report: Dmel\Egfr[DN.UAS.cBa]

General Information

Symbol

Dmel\Egfr^DN.UAS.cBa

Species

D. melanogaster

Name

dominant negative

FlyBase ID

FBal0090688

Feature type

allele

Associated gene

Dmel\Egfr

Associated Insertion(s)

Carried in Construct

P{UAS-Egfr.DN.B}

Also Known As

UAS-EGFR^DN, UAS-Egfr-DN, UAS-dnEGFR, UAS-DN-Egfr, UAS-DNDER, UAS-EGFRDN, UAS-DER^DN, Egfr^DN, UAS-Egfr^DN.B

Key Links

Transgenic product class

dominant_negative_variant

(Buff et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Buff et al., 1998)

Mutagen

in vitro construct

(Buff et al., 1998, Perkins et al., 1996)

Progenitor genotype

Carried in construct

P{UAS-Egfr.DN.B}

Cytology

Description

A dominant negative form of Egfr, with a stop codon introduced 20 amino acids C-terminal to the transmembrane domain, is expressed under the control of UASt regulatory sequences.

(Buff et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Egfr

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal learning | larval stage, with Scer\GAL4^ey-OK107

(Rahn et al., 2013)

abnormal learning | larval stage, with Scer\GAL4^nSyb.PS

(Rahn et al., 2013)

abnormal learning | larval stage | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

(Rahn et al., 2013)

abnormal neuroanatomy, with Scer\GAL4^GMR84B12

(Hernández et al., 2015)

abnormal neuroanatomy, with Scer\GAL4^{neur-GAL4-A101}

(Karim and Moore, 2011)

abnormal neuroanatomy, with Scer\GAL4^Ret.P2

(Hernández et al., 2015)

abnormal size, with Scer\GAL4^MD-638

(Molnar et al., 2011)

abnormal wound healing, with Scer\GAL4^69B

(Shen et al., 2013)

abnormal wound healing, with Scer\GAL4^c381

(Shen et al., 2013)

abnormal wound healing, with Scer\GAL4^LE

(Shen et al., 2013)

abnormal wound healing, with Scer\GAL4^ptc-559.1

(Shen et al., 2013)

decreased body size | male limited, with Scer\GAL4^elav.PU

(Yu et al., 2012)

decreased body size | male limited, with Scer\GAL4^Ilp2.PR

(Yu et al., 2012)

decreased cell death | heat sensitive, with Scer\GAL4^wg-IS650, Scer\GAL80^ts.αTub84B

(Takemura and Adachi-Yamada, 2011)

decreased cell number | adult stage | somatic clone, with Scer\GAL4^Tub.PU

(Gervais et al., 2019, Andriatsilavo et al., 2018)

decreased cell number | embryonic stage, with Scer\GAL4^how-24B, Scer\GAL4^twi.2PE

(Schwarz et al., 2018)

decreased cell number | third instar larval stage, with Scer\GAL4^nrv2.PS

(Matzat et al., 2015)

decreased cell number | third instar larval stage | conditional, with Scer\GAL4^HHLT

(Sinenko et al., 2011)

increased cell number | adult stage, with Scer\GAL4^C587

(Liu et al., 2015)

viable, with Scer\GAL4^sca-C253

(Perkins et al., 1996)

visible, with Scer\GAL4^71B

(Butchar et al., 2012)

visible, with Scer\GAL4^ap-md544

(Escudero et al., 2003, Culi et al., 2001)

visible, with Scer\GAL4^Bx-MS1096

(Guichard et al., 1999)

visible, with Scer\GAL4^c591

(Amin et al., 1999)

visible, with Scer\GAL4^dpp.3KK

(Molnar et al., 2006)

visible, with Scer\GAL4^GMR.PF

(Alvarado et al., 2004)

visible, with Scer\GAL4^MD-638

(Molnar et al., 2011)

visible, with Scer\GAL4^sca-C253

(Perkins et al., 1996)

visible | adult stage, with Scer\GAL4^MD-638

(Molnar and de Celis, 2013)

Phenotype Manifest In

abdominal dorsolateral adult muscle precursor cell, with Scer\GAL4^twi.PG

(Figeac et al., 2010)

abdominal lateral adult muscle precursor cell, with Scer\GAL4^twi.PG

(Figeac et al., 2010)

adult cuticle & head capsule | dorsal, with Scer\GAL4^c591

(Amin et al., 1999)

adult gut, with Scer\GAL4^NP0001

(Buchon et al., 2010)

adult gut, with Scer\GAL4^NP0001, Scer\GAL80^ts.αTub84B

(Buchon et al., 2010)

adult heart | heat sensitive, with Scer\GAL4^tin.CΔ4, Scer\GAL80^ts.αTub84B

(Yu et al., 2010)

adult midgut epithelium | adult stage | somatic clone, with Scer\GAL4^Tub.PU

(Gervais et al., 2019)

adult midgut epithelium | somatic clone, with Scer\GAL4^Tub.PU

(Andriatsilavo et al., 2018)

amnioserosa, with Scer\GAL4^69B

(Shen et al., 2013)

amnioserosa, with Scer\GAL4^c381

(Shen et al., 2013)

amnioserosa, with Scer\GAL4^LE

(Shen et al., 2013)

amnioserosa, with Scer\GAL4^ptc-559.1

(Shen et al., 2013)

anterior dorsocentral bristle, with Scer\GAL4^ap-md544

(Culi et al., 2001)

anterior postalar bristle, with Scer\GAL4^ap-md544

(Culi et al., 2001)

anterior scutellar bristle, with Scer\GAL4^ap-md544

(Culi et al., 2001)

anterior supraalar bristle, with Scer\GAL4^ap-md544

(Culi et al., 2001)

axon & mechanosensory neuron & adult head, with Scer\GAL4^unspecified

(Garcia-Alonso et al., 2000)

axon & ocellus sensory structure, with Scer\GAL4^sca-537.4

(Garcia-Alonso et al., 2000)

dorsal appendage, with Scer\GAL4^55B

(Wahlstrom et al., 2006)

egg, with Scer\GAL4^T155

(Perkins et al., 1996)

egg chorion, with Scer\GAL4^CY2

(Yakoby et al., 2008, Zartman et al., 2008, Alvarado et al., 2004)

embryonic/larval CNS subperineurial glial cell | third instar larval stage, with Scer\GAL4^moody.SPG

(Matzat et al., 2015)

embryonic/larval cuticle, with Scer\GAL4^69B

(Shen et al., 2013)

embryonic/larval cuticle, with Scer\GAL4^c381

(Shen et al., 2013)

embryonic/larval cuticle, with Scer\GAL4^LE

(Shen et al., 2013)

embryonic/larval cuticle, with Scer\GAL4^ptc-559.1

(Shen et al., 2013)

embryonic/larval frontal nerve, with Scer\GAL4^GMR84B12

(Hernández et al., 2015)

embryonic/larval frontal nerve, with Scer\GAL4^Ret.P2

(Hernández et al., 2015)

embryonic/larval ganglionic tracheal branch, with Scer\GAL4^btl.PS

(Iordanou et al., 2014)

embryonic/larval PNS perineurial glial cell | third instar larval stage, with Scer\GAL4^nrv2.PS

(Matzat et al., 2015)

embryonic/larval recurrent nerve, with Scer\GAL4^GMR84B12

(Hernández et al., 2015)

embryonic/larval recurrent nerve, with Scer\GAL4^Ret.P2

(Hernández et al., 2015)

embryonic/larval tracheal section, with Scer\GAL4^btl.PS

(Ruiz et al., 2012)

embryonic/larval wrapping glial cell | third instar larval stage, with Scer\GAL4^nrv2.PS

(Matzat et al., 2015)

embryonic/larval wrapping glial cell | third instar larval stage, with Scer\GAL4^repo.PL

(Matzat et al., 2015)

embryonic cardioblast, with Scer\GAL4^how-24B, Scer\GAL4^twi.2PE

(Schwarz et al., 2018)

embryonic head, with Scer\GAL4^69B

(Shen et al., 2013)

embryonic head, with Scer\GAL4^c381

(Shen et al., 2013)

embryonic head, with Scer\GAL4^LE

(Shen et al., 2013)

embryonic head, with Scer\GAL4^ptc-559.1

(Shen et al., 2013)

embryonic pericardial cell, with Scer\GAL4^twi.2PE

(Schwarz et al., 2018)

enterocyte, with Scer\GAL4^NP0001, Scer\GAL80^ts.αTub84B

(Buchon et al., 2010)

eye, with Scer\GAL4^c591

(Amin et al., 1999)

eye, with Scer\GAL4^GMR.PF

(Alvarado et al., 2004)

eye, with Scer\GAL4^sca-C253

(Perkins et al., 1996)

eye disc | third instar larval stage, with Scer\GAL4^ato.3'FL

(Yu et al., 2015)

eye disc | third instar larval stage, with Scer\GAL4^ato.5'EYE3'

(Yu et al., 2015)

eye disc | third instar larval stage, with Scer\GAL4^ato.5'EYE

(Yu et al., 2015)

filzkorper, with Scer\GAL4^ems.HRE

(Merabet et al., 2005)

germinal proliferation center | adult stage, with Scer\GAL4^C587

(Liu et al., 2015)

heart primordium, with Scer\GAL4^how-24B, Scer\GAL4^twi.2PE

(Schwarz et al., 2018)

heart primordium, with Scer\GAL4^twi.2PE

(Schwarz et al., 2018)

intestinal stem cell | adult stage | somatic clone, with Scer\GAL4^Tub.PU

(Gervais et al., 2019)

intestinal stem cell of posterior adult midgut epithelium, with Scer\GAL4^{Delta-05151-G}

(Zhou et al., 2013)

lamellocyte | larval stage | conditional, with Scer\GAL4^HHLT

(Sinenko et al., 2011)

lamellocyte | third instar larval stage | conditional, with Scer\GAL4^HHLT

(Sinenko et al., 2011)

larval somatic muscle cell, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG

(Buff et al., 1998)

larval ventral multidendritic neuron vdaB, with Scer\GAL4^{neur-GAL4-A101}

(Karim and Moore, 2011)

macrochaeta, with Scer\GAL4^ap-md544

(Escudero et al., 2003)

male germline cell | adult stage, with Scer\GAL4^C587

(Liu et al., 2015)

midgut, with Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B

(Buchon et al., 2010)

muscle cell of abdominal 2 dorsal acute muscle 1, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG

(Buff et al., 1998)

muscle cell of abdominal 2 ventral acute muscle 2, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG

(Buff et al., 1998)

muscle cell of abdominal 3 dorsal acute muscle 1, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG

(Buff et al., 1998)

muscle cell of abdominal 3 ventral acute muscle 2, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG

(Buff et al., 1998)

muscle cell of abdominal 4 dorsal acute muscle 1, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG

(Buff et al., 1998)

muscle cell of abdominal 4 ventral acute muscle 2, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG

(Buff et al., 1998)

muscle cell of abdominal 5 dorsal acute muscle 1, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG

(Buff et al., 1998)

muscle cell of abdominal 5 ventral acute muscle 2, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG

(Buff et al., 1998)

muscle cell of abdominal 6 dorsal acute muscle 1, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG

(Buff et al., 1998)

muscle cell of abdominal 6 ventral acute muscle 2, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG

(Buff et al., 1998)

muscle cell of abdominal 7 dorsal acute muscle 1, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG

(Buff et al., 1998)

muscle cell of abdominal 7 ventral acute muscle 2, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG

(Buff et al., 1998)

muscle cell of ventral acute muscle 2 | precursor, with Scer\GAL4^twi.PG

(Johnson Hamlet and Perkins, 2001)

ocellar bristle, with Scer\GAL4^c591

(Amin et al., 1999)

ocellus, with Scer\GAL4^c591

(Amin et al., 1999)

ommatidium, with Scer\GAL4^GMR.PF

(Alvarado et al., 2004)

photoreceptor neuron | third instar larval stage, with Scer\GAL4^ato.3'FL

(Yu et al., 2015)

photoreceptor neuron | third instar larval stage, with Scer\GAL4^ato.5'EYE3'

(Yu et al., 2015)

photoreceptor neuron | third instar larval stage, with Scer\GAL4^ato.5'EYE

(Yu et al., 2015)

posterior postalar bristle, with Scer\GAL4^ap-md544

(Culi et al., 2001)

posterior scutellar bristle, with Scer\GAL4^ap-md544

(Culi et al., 2001)

posterior supraalar bristle, with Scer\GAL4^ap-md544

(Culi et al., 2001)

stigmatophore, with Scer\GAL4^salm-459.2

(Merabet et al., 2005)

stomatogastric nervous system anlage, with Scer\GAL4^Gsc-SNS1

(Gonzalez-Gaitan and Jackle, 2000)

testis | adult stage, with Scer\GAL4^C587

(Liu et al., 2015)

tracheal lumen, with Scer\GAL4^btl.PS

(Ruiz et al., 2012)

wing, with Scer\GAL4^Bx-MS1096

(Guichard et al., 2002, Guichard et al., 1999)

wing blade, with Scer\GAL4^MD-638

(Molnar et al., 2011)

wing hair | heat sensitive, with Scer\GAL4^wg-IS650, Scer\GAL80^ts.αTub84B

(Takemura and Adachi-Yamada, 2011)

wing margin | heat sensitive, with Scer\GAL4^wg-IS650, Scer\GAL80^ts.αTub84B

(Takemura and Adachi-Yamada, 2011)

wing vein, with Scer\GAL4^71B

(Butchar et al., 2012)

wing vein, with Scer\GAL4^Bx-MS1096

(Guichard et al., 2002, Guichard et al., 1999)

wing vein, with Scer\GAL4^dpp.3KK

(Molnar et al., 2006)

wing vein, with Scer\GAL4^MD-638

(Molnar and de Celis, 2013, Molnar et al., 2011)

wing vein L3, with Scer\GAL4^Bx-MS1096

(Guichard et al., 1999)

wing vein L4, with Scer\GAL4^Bx-MS1096

(Guichard et al., 1999)

wing vein L5, with Scer\GAL4^Bx-MS1096

(Guichard et al., 1999)

Detailed Description

Statement

Reference

Somatic clones expressing Egfr^DN.UAS.cBa under the control of Scer\GAL4^Tub.PU contain a smaller overall number of cells and intestinal stem cells in adult flies compared to controls, but maintain a wild-type ratio of stem cells/clone.

(Gervais et al., 2019)

Adulthood-generated midgut clones expressing Egfr^DN.UAS.cBa under the control of Scer\GAL4^Tub.PU have significantly fewer cells, but insignificant changes in the number and proportion of intestinal stem cells, as compared to control clones.

(Andriatsilavo et al., 2018)

Expressing Egfr^DN.UAS.cBa under the double control of Scer\GAL4^twi.2PE and Scer\GAL4^how-24B leads to embryos showing severely decreased numbers of cardioblasts, namely of generic cardioblasts, leading to a lower generic CBs:ostial CBs ratio than the typical 4:2 ratio.

Expressing Egfr^DN.UAS.cBa under the control of Scer\GAL4^twi.2PE leads to embryos showing a significant decrease in the number of odd pericardial cells, although even pericardial cells are unaffected, as compared to controls.

(Schwarz et al., 2018)

Expression of Egfr^{DN.Scer\UAS.cBa} under the control of either Scer\GAL4^Ret.P2 or Scer\GAL4^GMR84B12 results in frequent loss or reduction of the frontal nerve and disruption of the recurrent nerve.

(Hernández et al., 2015)

Expression of Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^C587 (in combination with Gal80[ts] to restrict expression to adulthood) leads to a substantial increase in the number of germline stem cells in the germinal proliferation center, as compared to controls.

(Liu et al., 2015)

Expression of Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^repo.PL (and using tub-Gal80[ts] to restrict the expression to larval stages) result in significantly reduced axon wrapping index (proportion of axon clusters wrapped by wrapping glia) and many axons are wrapped in abnormally large fascicles, expression under Scer\GAL4^nrv2.PS also reduces the wrapping index but the number of wrapping glial cells along the nerve is unchanged whereas the number of perineurial glial cells is robustly decreased in third instar larvae.

In wild-type larvae, subperineurial glial cells (SPGs) form autocellular contacts with pronounced septate junction, the length of these autocellular contacts is significantly reduced upon Scer\GAL4^moody.SPG-driven (in SPGs) knock-down of vn.

(Matzat et al., 2015)

Expression of Egfr^{DN.Scer\UAS.cBa} under the control of either Scer\GAL4^ato.3'FL or Scer\GAL4^ato.5'EYE results in loss or delay in photoreceptor neurons recruitment in the developing eye disc. Expression under the Scer\GAL4^ato.5'EYE3' driver has even stronger effect and leads to a severe failure in photoreceptor neuron recruitment.

(Yu et al., 2015)

Scer\GAL4^btl.PS-mediated expression of Egfr^{DN.Scer\UAS.cBa} results in shorter tracheal ganglionic branches.

(Iordanou et al., 2014)

Larvae expressing Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^n-syb.PS show a significantly lower learning index compared to control larvae in an olfactory learning assay. These larvae show normal gustatory preference responses to fructose and to quinine and show normal olfactory responses to amyl acetate and to 1-octanol.

Expression of Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^{elav.Switch.PO} for just 24 hours in late larval stages (using mifepristone to control the timing of expression) results in significantly reduced olfactory learning ability in larvae.

Larvae expressing Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^ey-OK107 show a significantly lower learning index compared to control larvae in an olfactory learning assay.

(Rahn et al., 2013)

Expression of Egfr^{DN.Scer\UAS.cBa} in the general epidermis and epidermal stripes, under the control of Scer\GAL4^69B and Scer\GAL4^ptc-559.1, results in bowed embryos with occasional dorsal holes or scabs. 100% of embryos expressing Egfr^{DN.Scer\UAS.cBa} with Scer\GAL4^ptc-559.1 exhibit a bowed phenotype and 10% have a dorsal hole or scab.

Expression of Egfr^{DN.Scer\UAS.cBa} during dorsal closure, under the control of Scer\GAL4^LE reslts in a bowed embryo phenotype in about a quarter of embryos. Bowed embryos and dorsal holes are also seen when Egfr^{DN.Scer\UAS.cBa} is expressed in the amnioserosa, under the control of Scer\GAL4^c381.

(Shen et al., 2013)

Expression of under the control of Scer\GAL4^Dl-05151-G (and using tub-Gal80[ts] to limit the stage of expression) diminishes the increase in the rate of intestinal stem cell division normally observed in the adult midgut upon bacterial infection challenge. This effect is not seen when Egfr^{DN.Scer\UAS.cBa} is expressed under the Scer\GAL4^esg.PU driver (with tub-Gal80[ts]).

(Zhou et al., 2013)

Flies expressing Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^71B show complete loss of wing veins.

(Butchar et al., 2012)

Expression of Egfr^{DN.Scer\UAS.cBa} in the tracheal system under the control of Scer\GAL4^btl.PS results in gas-filling defects in the terminal cells.

(Ruiz et al., 2012)

Male Scer\GAL4^elav.PU>Egfr^{DN.Scer\UAS.cBa} flies have an approximately 13% body weight decrease.

Flies expressing Egfr^{DN.Scer\UAS.cBa} using Scer\GAL4^Ilp2.PR show no alterations in the morphology and number of insulin producing cells compared to controls.

Expression of Egfr^{DN.Scer\UAS.cBa} in insulin producing cells under the control of Scer\GAL4^Ilp2.PR results in reduced body weight in males.

(Yu et al., 2012)

Expression of Egfr^{DN.Scer\UAS.cBa} in stage 16 embryos, under the control of Scer\GAL4^{neur-GAL4-A101} results in the loss of vdaB in 75% of parasegments. There is no loss of v'ada. Examination of stage 12 embryos reveals that vdaB-loss is preceded by SOP1a loss in 78% of cases. SOP4a are not affected.

(Karim and Moore, 2011)

Expression of Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^MD-638 causes the loss of wing veins and reduction in wing size.

(Molnar et al., 2011)

Cells expressing Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^HHLT are unable to become lamellocytes following wasp infestation.

(Sinenko et al., 2011)

Expression of Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^HHLT reduces the increase in lamellocyte number seen following wasp infestation.

(Sinenko et al., 2011)

Induction of Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^wg-IS650 during 0-30 hours after pupal formation blocks apoptosis and rearranges the wing margin cells. In addition, blocking ecdysone signalling results in overlap of the double-row hairs in the adult wing.

(Takemura and Adachi-Yamada, 2011)

Expression of Egfr^{DN.Scer\UAS.cBa} in intestinal stem cells (ISCs) driven by Scer\GAL4^esg-NP5130 and Scer\GAL80^ts.αTub84B does not significantly affect the number of ISCs in the gut. However, in sharp contrast to wild-type flies, Erwinia carotovora carotovora 15 (Ecc15) infection does not increase the number of intestinal mitotic cells.

Guts from flies expressing Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^{Myo31DF-NP0001} are almost double the length and half the width compared with the guts of wild-type flies. However, the total number of enterocytes is the same as it is in wild-type controls. The alteration in gut length correlates with changes in the shape of enterocytes. In contrast to wild-type enterocytes, the mutant enterocytes appear flattened and lack the extension of their apical domain. In addition, the distance between nuclei of neighbouring enterocytes (indicative of epithelial density) increases twofold in these flies and altered in its axis. This effect on cell shape is more pronounced in certain gut segments, particularly around the copper cell region which is normally composed of a highly polarised columnar-type epithelium and is folded within the abdomen. As a consequence, guts of flies expressing Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^{Myo31DF-NP0001} are long and thin in this region and lack their characteristic folding.

Flies expressing Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^{Myo31DF-NP0001} succumb within 48 hours after ingesting Erwinia carotovora carotovora 15 (Ecc15). This increased susceptibility is associated with a rupture in gut integrity, as indicated by the presence of bacteria in the hemolymph.

Guts of flies overexpressing Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^{Myo31DF-NP0001} and Scer\GAL80^ts.αTub84B shifted to the restrictive temperature for 4 to 5 days are initially of normal size. A significant increase in gut length is observed when flies are incubated for 3 weeks at the restrictive temperature.

Guts of flies overexpressing Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^{Myo31DF-NP0001} and Scer\GAL80^ts.αTub84B are 20% longer compared with guts wild-type flies 48 hours post-infection with Erwinia carotovora carotovora 15 (Ecc15).

Guts of flies expressing Egfr^{DN.Scer\UAS.cBa} in enterocytes under the control of Scer\GAL4^{Myo31DF-NP0001} and Scer\GAL80^ts.αTub84B shrink less upon infection with Erwinia carotovora carotovora 15 (Ecc15). After a recovery phase of 2 or 8 days, the guts are 25% longer than their wild-type counterparts.

In contrast to wild-type flies, cell blebbing, the multi-layering of enterocytes or loss of cells into the lumen of flies are not observed in flies expressing Egfr^{DN.Scer\UAS.cBa} in enterocytes under the control of Scer\GAL4^{Myo31DF-NP0001} and Scer\GAL80^ts.αTub84B. In sharp contrast to wild-type enterocytes, apoptotic enterocytes with fragmented nuclei are observed within the epithelium of flies expressing Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^{Myo31DF-NP0001} and Scer\GAL80^ts.αTub84B.

(Buchon et al., 2010)

Embryos expressing Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^twi.PG show a decrease in the number of dorsolateral and lateral adult muscle precursor cells compared to wild type.

(Figeac et al., 2010)

The cardiac function of flies expressing Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^tin.CΔ4 and Scer\GAL80^ts.αTub84B is normal at 18[o]C, but, compared with controls, it progressively deteriorates at 25[o]C as manifested by enlarged end-diastolic (EDD) and end-systolic dimensions (ESD). The Egfr^{DN.Scer\UAS.cBa}-mediated effects on cardiac chamber size are reversible when Egfr^{DN.Scer\UAS.cBa} expression is repressed by temperature shift.

(Yu et al., 2010)

Expression of Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^CY2 abolishes the dorsal eggshell structures.

(Yakoby et al., 2008)

Expression of Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^CY2 results in a ventralised eggshell.

(Zartman et al., 2008)

Expression of Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^dpp.3KK results in loss of wing veins.

(Molnar et al., 2006)

Expression of Egfr^{DN.Scer\UAS.cBa} in the anterior cells of the egg chamber, under the control of Scer\GAL4^55B, results in eggs with a single fused dorsal appendage or with a patch of dorsal appendage material.

(Wahlstrom et al., 2006)

Expression of Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4¹¹⁵¹ does not affect founder cell formation of patterning.

(Dutta et al., 2005)

Expression of Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^ems.HRE impairs filzkorper formation but does not affect stigmatophore formation. However, expression of this transgene under the control of Scer\GAL4^salm-459.2 has the reciprocal effect; stigmatophore formation is impaired while filzkorper formation is unaffected.

(Merabet et al., 2005)

Expression of Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^CY2 results in eggs with strongly ventralised chorions. Expression of Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^GMR.PF results in a severe rough eye phenotype. The rough eye phenotype caused by expression of Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^GMR.PF is not suppressed by Egfr^SOK2/+.

(Alvarado et al., 2004)

The number of macrochaetae/heminotum in animals expressing Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^ap-md544 is - anterior+posterior notopleural: 1.61, presutural: 0.70, anterior supraalar: 0.50, posterior supraalar: 0, anterior postalar: 0, posterior postalar: 0, anterior+posterior dorsocentral: 0.78 and anterior+posterior scutellar: 1.55.

(Escudero et al., 2003)

Egfr^{DN.Scer\UAS.cBa}; Scer\GAL4^Bx-MS1096 flies have small curved wings with vein truncations.

(Guichard et al., 2002)

When expression is driven by Scer\GAL4^ap-md544 most notum macrochaetae are removed, though microchaetae are unaffected.

(Culi et al., 2001)

Embryos expressing Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^twi.PG show loss of VA2 muscle precursor cells (only 26% of hemisegments contain VA2 precursor cells).

(Johnson Hamlet and Perkins, 2001)

Expression of Egfr^{DN.Scer\UAS.cBa} can result in loss of sensory organs in the ocellar sensory system (OSS), unless raised at 17^oC, which allows some differentiation of these neurons. In these conditions, some axon guidance defects are seen in OSS neurons. The most common alteration (in about 1/3 of animals) is bristle mechanosensory (BM) axons stalling in the epidermis. Alterations in Ocellar pioneer (OP) axon guidance are much less frequent.

(Garcia-Alonso et al., 2000)

Expression of Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^Gsc-SNS1 results in suppression of the formation of the anterior most invagination fold in the stomatogastric nervous system anlage, without affecting the others, in stage 11 embryos.

(Gonzalez-Gaitan and Jackle, 2000)

When expressed under the control of Scer\GAL4^c591, the ocelli and the ocellar bristles are deleted. However, most of the interocellar cuticle is retained.

(Amin et al., 1999)

Expression of Egfr^{DN.Scer\UAS.cBa} driven by Scer\GAL4^Bx-MS1096 deletes parts of dorsal veins L3, L5 and the distal portion of L4, reduces the width of the wing, and causes mis-alignment between dorsal and ventral components of the veins.

(Guichard et al., 1999)

The ventral muscle founder cells are specified in embryos expressing Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^twi.PB.

(Schulz and Gajewski, 1999)

Many myofibres are missing from all muscle groups in embryos expressing Egfr^{DN.Scer\UAS.cBa} under the control of both Scer\GAL4^twi.PG and Scer\GAL4^how-24B. The precursor of muscles DA1 is missing. Muscles DO1, LT2 and LT4, and the eve-expressing pericardial cell precursors form normally. Apoptosis within the mesoderm is significantly higher than in wild-type embryos. Embryos expressing Egfr^{DN.Scer\UAS.cBa} under the control of both Scer\GAL4^twi.PG and Scer\GAL4^how-24B show a partial reduction in the development of muscles DA1 and VA2. This phenotype is suppressed by co-expression of Egfr^Scer\UAS.cBa. The P15 muscle precursor cell does not form in embryos expressing Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^twi.PG. Embryos expressing rho^Scer\UAS.cBa under the control of Scer\GAL4^twi.PG show a marked overproduction of eve-positive mesodermal founder cells. This phenotype is partly suppressed if the embryos are also carrying Egfr^{DN.Scer\UAS.cBa}.

(Buff et al., 1998)

Scer\GAL4^sca-C253 mediated expression generates flies with reduced eyes. Females carrying Scer\GAL4^T155 and Egfr^{DN.Scer\UAS.cBa} lay partially to completely ventralised eggs.

(Perkins et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Egfr^DN.UAS.cBa, Scer\GAL4^T155 has visible phenotype, enhanceable by csw^LE120

(Perkins et al., 1996)

Egfr^DN.UAS.cBa, Scer\GAL4^T155 has visible phenotype, enhanceable by csw⁶

(Perkins et al., 1996)

NOT Enhanced by

Statement

Reference

Egfr^DN.UAS.cBa, Scer\GAL4^MD-638 has visible | adult stage phenotype, non-enhanceable by tay^RNAi.UAS, Scer\GAL4^MD-638

(Molnar and de Celis, 2013)

Egfr^DN.UAS.cBa, Scer\GAL4^MD-638 has visible phenotype, non-enhanceable by krz^RNAi.UAS, Scer\GAL4^MD-638

(Molnar et al., 2011)

Egfr^DN.UAS.cBa, Scer\GAL4^MD-638 has abnormal size phenotype, non-enhanceable by krz^RNAi.UAS, Scer\GAL4^MD-638

(Molnar et al., 2011)

Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096 has visible phenotype, non-enhanceable by rho^UAS.cBa, Scer\GAL4^Bx-MS1096

(Guichard et al., 1999)

Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096 has visible phenotype, non-enhanceable by rho^UAS.cBa/vn^UAS.cSa, Scer\GAL4^Bx-MS1096

(Guichard et al., 1999)

Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096 has visible phenotype, non-enhanceable by rho^UAS.cBa/S^UAS.cGb, Scer\GAL4^Bx-MS1096

(Guichard et al., 1999)

NOT suppressed by

Statement

Reference

Egfr^DN.UAS.cBa, Scer\GAL4^MD-638 has visible phenotype, non-suppressible by krz^RNAi.UAS, Scer\GAL4^MD-638

(Molnar et al., 2011)

Egfr^DN.UAS.cBa, Scer\GAL4^MD-638 has abnormal size phenotype, non-suppressible by krz^RNAi.UAS, Scer\GAL4^MD-638

(Molnar et al., 2011)

Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096 has visible phenotype, non-suppressible by rho^UAS.cBa, Scer\GAL4^Bx-MS1096

(Guichard et al., 1999)

Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096 has visible phenotype, non-suppressible by rho^UAS.cBa/vn^UAS.cSa, Scer\GAL4^Bx-MS1096

(Guichard et al., 1999)

Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096 has visible phenotype, non-suppressible by rho^UAS.cBa/S^UAS.cGb, Scer\GAL4^Bx-MS1096

(Guichard et al., 1999)

NOT Enhancer of

Statement

Reference

Scer\GAL4^Tub.PU/Egfr^DN.UAS.cBa is a non-enhancer of increased cell number | adult stage | somatic clone phenotype of spen⁵

(Andriatsilavo et al., 2018)

Scer\GAL4^insc-Mz1407/Egfr^DN.UAS.cBa is a non-enhancer of increased cell number | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Scer\GAL4^insc-Mz1407/Egfr^DN.UAS.cBa is a non-enhancer of abnormal neuroanatomy | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Suppressor of

Statement

Reference

Egfr^DN.UAS.cBa, Scer\GAL4^NP0001 is a suppressor of increased occurrence of cell division | adult stage phenotype of Rab21^GD9402, Scer\GAL4^NP0001

(Nassari et al., 2022)

Egfr^DN.UAS.cBa, Scer\GAL4^NP0001 is a suppressor of increased occurrence of cell division | adult stage phenotype of Rab21^KK115582, Scer\GAL4^NP0001

(Nassari et al., 2022)

Egfr^DN.UAS.cBa, Scer\GAL4^NP0001 is a suppressor of increased occurrence of cell division | adult stage phenotype of Rab21^T27N.UAS.GFP, Scer\GAL4^NP0001

(Nassari et al., 2022)

Egfr^DN.UAS.cBa, Scer\GAL4^bun-GSG5961 is a suppressor of increased occurrence of cell division | adult stage phenotype of Nrg^167.UAS, Scer\GAL4^bun-GSG5961

(Resnik-Docampo et al., 2021)

Scer\GAL4^Tub.PU/Egfr^DN.UAS.cBa is a suppressor of increased cell number | recessive | somatic clone | adult stage phenotype of kis^10D26

(Gervais et al., 2019)

Egfr^DN.UAS.cBa, Scer\GAL4^Tub.PU is a suppressor of increased cell number | somatic clone | adult stage phenotype of Scer\GAL4^Tub.PU, trr^JF03242

(Gervais et al., 2019)

Scer\GAL4^NP6267/Egfr^DN.UAS.cBa is a suppressor of increased occurrence of cell division | adult stage phenotype of Sulf1^KO.Cherry/Sulf1^ΔP1

(Takemura and Nakato, 2017)

Egfr^DN.UAS.cBa, Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B is a suppressor | partially of neoplasia phenotype of Sox21a⁶

(Zhai et al., 2015)

Egfr^DN.UAS.cBa, Scer\GAL4^elav.PU is a suppressor of increased body size | male limited phenotype of Cbl^GD12111, Scer\GAL4^elav.PU

(Yu et al., 2012)

Egfr^DN.UAS.cBa, Scer\GAL4^Ilp2.PR is a suppressor of increased body size | male limited phenotype of Cbl^GD12111, Scer\GAL4^Ilp2.PR

(Yu et al., 2012)

Egfr^DN.UAS.cBa, Scer\GAL4^NP5130 is a suppressor of increased occurrence of cell division | adult stage phenotype of Scer\GAL4^NP5130, upd3^UAS.cMa

(Buchon et al., 2010)

Scer\GAL4^ap-md544/Egfr^DN.UAS.cBa is a suppressor | partially of visible phenotype of ed^slH8/ed^1X5

(Escudero et al., 2003)

Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096 is a suppressor of visible phenotype of Scer\GAL4^Bx-MS1096, rho^UAS.cBa

(Guichard et al., 1999)

NOT Suppressor of

Statement

Reference

Egfr^DN.UAS.cBa, Scer\GAL4^NP0001 is a non-suppressor of increased occurrence of cell division | adult stage phenotype of Scer\GAL4^NP0001, Syx17^JF01937

(Nassari et al., 2022)

Egfr^DN.UAS.cBa, Scer\GAL4^NP0001 is a non-suppressor of increased occurrence of cell division | adult stage phenotype of Scer\GAL4^NP0001, Vamp7^NIG.1599R

(Nassari et al., 2022)

Scer\GAL4^Tub.PU/Egfr^DN.UAS.cBa is a non-suppressor of increased cell number | somatic clone | adult stage phenotype of spen⁵

(Andriatsilavo et al., 2018)

Scer\GAL4^insc-Mz1407/Egfr^DN.UAS.cBa is a non-suppressor of increased cell number | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Scer\GAL4^insc-Mz1407/Egfr^DN.UAS.cBa is a non-suppressor of abnormal neuroanatomy | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Phenotype Manifest In

Enhanced by

Statement

Reference

Egfr^DN.UAS.cBa, Scer\GAL4^how-24B, Scer\GAL4^twi.PG has muscle cell of abdominal 2 dorsal acute muscle 1 phenotype, enhanceable by S[+]/S^IIN

(Buff et al., 1998)

Egfr^DN.UAS.cBa, Scer\GAL4^how-24B, Scer\GAL4^twi.PG has muscle cell of abdominal 3 dorsal acute muscle 1 phenotype, enhanceable by S[+]/S^IIN

(Buff et al., 1998)

Egfr^DN.UAS.cBa, Scer\GAL4^how-24B, Scer\GAL4^twi.PG has muscle cell of abdominal 4 dorsal acute muscle 1 phenotype, enhanceable by S[+]/S^IIN

(Buff et al., 1998)

Egfr^DN.UAS.cBa, Scer\GAL4^how-24B, Scer\GAL4^twi.PG has muscle cell of abdominal 5 dorsal acute muscle 1 phenotype, enhanceable by S[+]/S^IIN

(Buff et al., 1998)

Egfr^DN.UAS.cBa, Scer\GAL4^how-24B, Scer\GAL4^twi.PG has muscle cell of abdominal 6 dorsal acute muscle 1 phenotype, enhanceable by S[+]/S^IIN

(Buff et al., 1998)

Egfr^DN.UAS.cBa, Scer\GAL4^how-24B, Scer\GAL4^twi.PG has muscle cell of abdominal 7 dorsal acute muscle 1 phenotype, enhanceable by S[+]/S^IIN

(Buff et al., 1998)

Egfr^DN.UAS.cBa, Scer\GAL4^how-24B, Scer\GAL4^twi.PG has muscle cell of abdominal 2 ventral acute muscle 2 phenotype, enhanceable by S[+]/S^IIN

(Buff et al., 1998)

Egfr^DN.UAS.cBa, Scer\GAL4^how-24B, Scer\GAL4^twi.PG has muscle cell of abdominal 3 ventral acute muscle 2 phenotype, enhanceable by S[+]/S^IIN

(Buff et al., 1998)

Egfr^DN.UAS.cBa, Scer\GAL4^how-24B, Scer\GAL4^twi.PG has muscle cell of abdominal 4 ventral acute muscle 2 phenotype, enhanceable by S[+]/S^IIN

(Buff et al., 1998)

Egfr^DN.UAS.cBa, Scer\GAL4^how-24B, Scer\GAL4^twi.PG has muscle cell of abdominal 5 ventral acute muscle 2 phenotype, enhanceable by S[+]/S^IIN

(Buff et al., 1998)

Egfr^DN.UAS.cBa, Scer\GAL4^how-24B, Scer\GAL4^twi.PG has muscle cell of abdominal 6 ventral acute muscle 2 phenotype, enhanceable by S[+]/S^IIN

(Buff et al., 1998)

Egfr^DN.UAS.cBa, Scer\GAL4^how-24B, Scer\GAL4^twi.PG has muscle cell of abdominal 7 ventral acute muscle 2 phenotype, enhanceable by S[+]/S^IIN

(Buff et al., 1998)

NOT Enhanced by

Statement

Reference

Egfr^DN.UAS.cBa, Scer\GAL4^MD-638 has wing vein phenotype, non-enhanceable by tay^RNAi.UAS, Scer\GAL4^MD-638

(Molnar and de Celis, 2013)

Egfr^DN.UAS.cBa, Scer\GAL4^MD-638 has wing blade phenotype, non-enhanceable by krz^RNAi.UAS, Scer\GAL4^MD-638

(Molnar et al., 2011)

Egfr^DN.UAS.cBa, Scer\GAL4^MD-638 has wing vein phenotype, non-enhanceable by krz^RNAi.UAS, Scer\GAL4^MD-638

(Molnar et al., 2011)

Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096 has wing phenotype, non-enhanceable by rho^UAS.cBa, Scer\GAL4^Bx-MS1096

(Guichard et al., 2000)

Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096 has wing phenotype, non-enhanceable by stet^UAS.cGa, Scer\GAL4^Bx-MS1096

(Guichard et al., 2000)

Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096, rho^UAS.cBa has wing vein L3 phenotype, non-enhanceable by vn^UAS.cSa, Scer\GAL4^Bx-MS1096

(Guichard et al., 1999)

Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096, rho^UAS.cBa has wing vein L4 phenotype, non-enhanceable by vn^UAS.cSa, Scer\GAL4^Bx-MS1096

(Guichard et al., 1999)

Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096, rho^UAS.cBa has wing vein L5 phenotype, non-enhanceable by vn^UAS.cSa, Scer\GAL4^Bx-MS1096

(Guichard et al., 1999)

Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096, rho^UAS.cBa has wing phenotype, non-enhanceable by S^UAS.cGb, Scer\GAL4^Bx-MS1096

(Guichard et al., 1999)

Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096, rho^UAS.cBa has wing vein phenotype, non-enhanceable by S^UAS.cGb, Scer\GAL4^Bx-MS1096

(Guichard et al., 1999)

Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096, rho^UAS.cBa has wing vein L3 phenotype, non-enhanceable by S^UAS.cGb, Scer\GAL4^Bx-MS1096

(Guichard et al., 1999)

Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096, rho^UAS.cBa has wing vein L4 phenotype, non-enhanceable by S^UAS.cGb, Scer\GAL4^Bx-MS1096

(Guichard et al., 1999)

Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096, rho^UAS.cBa has wing vein L5 phenotype, non-enhanceable by S^UAS.cGb, Scer\GAL4^Bx-MS1096

(Guichard et al., 1999)

Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096, rho^UAS.cBa has wing phenotype, non-enhanceable by vn^UAS.cSa, Scer\GAL4^Bx-MS1096

(Guichard et al., 1999)

Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096, rho^UAS.cBa has wing vein phenotype, non-enhanceable by vn^UAS.cSa, Scer\GAL4^Bx-MS1096

(Guichard et al., 1999)

Suppressed by

Statement

Reference

Egfr^DN.UAS.cBa, Scer\GAL4^twi.PG has muscle cell of ventral acute muscle 2 | precursor phenotype, suppressible | partially by csw^{UAS.Tag:Myr(Src64B)}, Scer\GAL4^twi.PG

(Johnson Hamlet and Perkins, 2001)

Egfr^DN.UAS.cBa, Scer\GAL4^how-24B, Scer\GAL4^twi.PG has muscle cell of abdominal 2 dorsal acute muscle 1 phenotype, suppressible by S^UAS.cBa, Scer\GAL4^how-24B, Scer\GAL4^twi.PG

(Buff et al., 1998)

Egfr^DN.UAS.cBa, Scer\GAL4^how-24B, Scer\GAL4^twi.PG has muscle cell of abdominal 3 dorsal acute muscle 1 phenotype, suppressible by S^UAS.cBa, Scer\GAL4^how-24B, Scer\GAL4^twi.PG

(Buff et al., 1998)

Egfr^DN.UAS.cBa, Scer\GAL4^how-24B, Scer\GAL4^twi.PG has muscle cell of abdominal 4 dorsal acute muscle 1 phenotype, suppressible by S^UAS.cBa, Scer\GAL4^how-24B, Scer\GAL4^twi.PG

(Buff et al., 1998)

Egfr^DN.UAS.cBa, Scer\GAL4^how-24B, Scer\GAL4^twi.PG has muscle cell of abdominal 5 dorsal acute muscle 1 phenotype, suppressible by S^UAS.cBa, Scer\GAL4^how-24B, Scer\GAL4^twi.PG

(Buff et al., 1998)

Egfr^DN.UAS.cBa, Scer\GAL4^how-24B, Scer\GAL4^twi.PG has muscle cell of abdominal 6 dorsal acute muscle 1 phenotype, suppressible by S^UAS.cBa, Scer\GAL4^how-24B, Scer\GAL4^twi.PG

(Buff et al., 1998)

Egfr^DN.UAS.cBa, Scer\GAL4^how-24B, Scer\GAL4^twi.PG has muscle cell of abdominal 7 dorsal acute muscle 1 phenotype, suppressible by S^UAS.cBa, Scer\GAL4^how-24B, Scer\GAL4^twi.PG

(Buff et al., 1998)

Egfr^DN.UAS.cBa, Scer\GAL4^how-24B, Scer\GAL4^twi.PG has muscle cell of abdominal 2 ventral acute muscle 2 phenotype, suppressible by S^UAS.cBa, Scer\GAL4^how-24B, Scer\GAL4^twi.PG

(Buff et al., 1998)

Egfr^DN.UAS.cBa, Scer\GAL4^how-24B, Scer\GAL4^twi.PG has muscle cell of abdominal 3 ventral acute muscle 2 phenotype, suppressible by S^UAS.cBa, Scer\GAL4^how-24B, Scer\GAL4^twi.PG

(Buff et al., 1998)

Egfr^DN.UAS.cBa, Scer\GAL4^how-24B, Scer\GAL4^twi.PG has muscle cell of abdominal 4 ventral acute muscle 2 phenotype, suppressible by S^UAS.cBa, Scer\GAL4^how-24B, Scer\GAL4^twi.PG

(Buff et al., 1998)

Egfr^DN.UAS.cBa, Scer\GAL4^how-24B, Scer\GAL4^twi.PG has muscle cell of abdominal 5 ventral acute muscle 2 phenotype, suppressible by S^UAS.cBa, Scer\GAL4^how-24B, Scer\GAL4^twi.PG

(Buff et al., 1998)

Egfr^DN.UAS.cBa, Scer\GAL4^how-24B, Scer\GAL4^twi.PG has muscle cell of abdominal 6 ventral acute muscle 2 phenotype, suppressible by S^UAS.cBa, Scer\GAL4^how-24B, Scer\GAL4^twi.PG

(Buff et al., 1998)

Egfr^DN.UAS.cBa, Scer\GAL4^how-24B, Scer\GAL4^twi.PG has muscle cell of abdominal 7 ventral acute muscle 2 phenotype, suppressible by S^UAS.cBa, Scer\GAL4^how-24B, Scer\GAL4^twi.PG

(Buff et al., 1998)

NOT suppressed by

Statement

Reference

Egfr^DN.UAS.cBa, Scer\GAL4^btl.PS has embryonic/larval ganglionic tracheal branch phenotype, non-suppressible by exp¹³⁵

(Iordanou et al., 2014)

Egfr^DN.UAS.cBa, Scer\GAL4^MD-638 has wing blade phenotype, non-suppressible by krz^RNAi.UAS, Scer\GAL4^MD-638

(Molnar et al., 2011)

Egfr^DN.UAS.cBa, Scer\GAL4^MD-638 has wing vein phenotype, non-suppressible by krz^RNAi.UAS, Scer\GAL4^MD-638

(Molnar et al., 2011)

Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096 has wing phenotype, non-suppressible by stet^UAS.cGa, Scer\GAL4^Bx-MS1096

(Guichard et al., 2000)

Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096 has wing phenotype, non-suppressible by rho^UAS.cBa, Scer\GAL4^Bx-MS1096

(Guichard et al., 2000)

Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096, rho^UAS.cBa has wing phenotype, non-suppressible by S^UAS.cGb, Scer\GAL4^Bx-MS1096

(Guichard et al., 1999)

Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096, rho^UAS.cBa has wing vein phenotype, non-suppressible by S^UAS.cGb, Scer\GAL4^Bx-MS1096

(Guichard et al., 1999)

Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096, rho^UAS.cBa has wing vein L3 phenotype, non-suppressible by S^UAS.cGb, Scer\GAL4^Bx-MS1096

(Guichard et al., 1999)

Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096, rho^UAS.cBa has wing vein L4 phenotype, non-suppressible by S^UAS.cGb, Scer\GAL4^Bx-MS1096

(Guichard et al., 1999)

Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096, rho^UAS.cBa has wing vein L5 phenotype, non-suppressible by S^UAS.cGb, Scer\GAL4^Bx-MS1096

(Guichard et al., 1999)

Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096, rho^UAS.cBa has wing phenotype, non-suppressible by vn^UAS.cSa, Scer\GAL4^Bx-MS1096

(Guichard et al., 1999)

Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096, rho^UAS.cBa has wing vein phenotype, non-suppressible by vn^UAS.cSa, Scer\GAL4^Bx-MS1096

(Guichard et al., 1999)

Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096, rho^UAS.cBa has wing vein L3 phenotype, non-suppressible by vn^UAS.cSa, Scer\GAL4^Bx-MS1096

(Guichard et al., 1999)

Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096, rho^UAS.cBa has wing vein L4 phenotype, non-suppressible by vn^UAS.cSa, Scer\GAL4^Bx-MS1096

(Guichard et al., 1999)

Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096, rho^UAS.cBa has wing vein L5 phenotype, non-suppressible by vn^UAS.cSa, Scer\GAL4^Bx-MS1096

(Guichard et al., 1999)

NOT Enhancer of

Statement

Reference

Scer\GAL4^Tub.PU/Egfr^DN.UAS.cBa is a non-enhancer of adult midgut epithelium | somatic clone phenotype of spen⁵

(Andriatsilavo et al., 2018)

Scer\GAL4^Tub.PU/Egfr^DN.UAS.cBa is a non-enhancer of intestinal stem cell | adult stage | somatic clone phenotype of spen⁵

(Andriatsilavo et al., 2018)

Scer\GAL4^insc-Mz1407/Egfr^DN.UAS.cBa is a non-enhancer of larval neuroblast | larval stage | increased number phenotype of Vps35^E42

(Li et al., 2018)

Scer\GAL4^insc-Mz1407/Egfr^DN.UAS.cBa is a non-enhancer of larval brain | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Suppressor of

Statement

Reference

Egfr^DN.UAS.cBa, Scer\GAL4^NP0001 is a suppressor of adult gut phenotype of Rab21^GD9402, Scer\GAL4^NP0001

(Nassari et al., 2022)

Egfr^DN.UAS.cBa, Scer\GAL4^NP0001 is a suppressor of adult gut phenotype of Rab21^KK115582, Scer\GAL4^NP0001

(Nassari et al., 2022)

Egfr^DN.UAS.cBa, Scer\GAL4^NP0001 is a suppressor of adult gut phenotype of Rab21^T27N.UAS.GFP, Scer\GAL4^NP0001

(Nassari et al., 2022)

Egfr^DN.UAS.cBa, Scer\GAL4^bun-GSG5961 is a suppressor of adult posterior midgut epithelium phenotype of Nrg^167.UAS, Scer\GAL4^bun-GSG5961

(Resnik-Docampo et al., 2021)

Scer\GAL4^Tub.PU/Egfr^DN.UAS.cBa is a suppressor of adult midgut epithelium | somatic clone phenotype of kis^10D26

(Gervais et al., 2019)

Scer\GAL4^Tub.PU/Egfr^DN.UAS.cBa is a suppressor of intestinal stem cell | somatic clone | adult stage phenotype of kis^10D26

(Gervais et al., 2019)

Egfr^DN.UAS.cBa, Scer\GAL4^Tub.PU is a suppressor of adult midgut epithelium | somatic clone | adult stage phenotype of Scer\GAL4^Tub.PU, trr^JF03242

(Gervais et al., 2019)

Egfr^DN.UAS.cBa, Scer\GAL4^Tub.PU is a suppressor of intestinal stem cell | somatic clone | adult stage phenotype of Scer\GAL4^Tub.PU, trr^JF03242

(Gervais et al., 2019)

Scer\GAL4^NP6267/Egfr^DN.UAS.cBa is a suppressor of adult posterior midgut epithelium phenotype of Sulf1^KO.Cherry/Sulf1^ΔP1

(Takemura and Nakato, 2017)

Egfr^DN.UAS.cBa, Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B is a suppressor | partially of adult anterior midgut epithelium phenotype of Sox21a⁶

(Zhai et al., 2015)

Scer\GAL4^btl.PS/Egfr^DN.UAS.cBa is a suppressor of embryonic/larval tracheal lateral trunk phenotype of exp¹³⁵

(Iordanou et al., 2014)

Scer\GAL4^btl.PS/Egfr^DN.UAS.cBa is a suppressor of embryonic/larval ganglionic tracheal branch phenotype of exp¹³⁵

(Iordanou et al., 2014)

Scer\GAL80^ts.αTub84B, Egfr^DN.UAS.cBa, Scer\GAL4^NP5130 is a suppressor of midgut phenotype of Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B, upd3^UAS.cMa

(Buchon et al., 2010)

Scer\GAL80^ts.αTub84B, Egfr^DN.UAS.cBa, Scer\GAL4^NP5130 is a suppressor of midgut phenotype of Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B, dome^UAS.EGFP

(Buchon et al., 2010)

Scer\GAL4^btl.PS/Egfr^DN.UAS.cBa is a suppressor of larval tracheal system phenotype of Ptp10D¹, Ptp4E¹

(Jeon and Zinn, 2009)

Egfr^DN.UAS.cBa, Scer\GAL4^CY2 is a suppressor of egg chorion phenotype of Egfr::kek1^{KEΔCG.UAS.GFP}, Scer\GAL4^CY2

(Alvarado et al., 2004)

Scer\GAL4^ap-md544/Egfr^DN.UAS.cBa is a suppressor | partially of macrochaeta | ectopic phenotype of ed^slH8/ed^1X5

(Escudero et al., 2003)

Egfr^DN.UAS.cBa/Scer\GAL4^Bx-MS1096 is a suppressor of wing vein | ectopic phenotype of Pstu\aarA^UAS.cGa

(Gallio et al., 2002)

Egfr^DN.UAS.cBa/Scer\GAL4^Bx-MS1096 is a suppressor of wing phenotype of Pstu\aarA^UAS.cGa

(Gallio et al., 2002)

Egfr^DN.UAS.cBa, Scer\GAL4^twi.PG is a suppressor | partially of muscle founder cell | embryonic stage phenotype of Scer\GAL4^twi.PG, rho^UAS.cBa

(Buff et al., 1998)

NOT Suppressor of

Statement

Reference

Egfr^DN.UAS.cBa, Scer\GAL4^NP0001 is a non-suppressor of adult gut phenotype of Scer\GAL4^NP0001, Syx17^JF01937

(Nassari et al., 2022)

Egfr^DN.UAS.cBa, Scer\GAL4^NP0001 is a non-suppressor of adult gut phenotype of Scer\GAL4^NP0001, Vamp7^NIG.1599R

(Nassari et al., 2022)

Scer\GAL4^Tub.PU/Egfr^DN.UAS.cBa is a non-suppressor of adult midgut epithelium | somatic clone phenotype of spen⁵

(Andriatsilavo et al., 2018)

Scer\GAL4^Tub.PU/Egfr^DN.UAS.cBa is a non-suppressor of intestinal stem cell | somatic clone | adult stage phenotype of spen⁵

(Andriatsilavo et al., 2018)

Scer\GAL4^insc-Mz1407/Egfr^DN.UAS.cBa is a non-suppressor of larval neuroblast | increased number | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Scer\GAL4^insc-Mz1407/Egfr^DN.UAS.cBa is a non-suppressor of larval brain | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

The increased division of intestinal stem cells in the adult posterior midgut during normal homeostasis characteristic for Sulf1^KO;Cherry/Sulf1^ΔP1 mutants can be suppressed by expression of Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^NP6267 (using tub-Gal80[ts] to limit the time of expression to adulthood).

(Takemura and Nakato, 2017)

Expression of Egfr^{DN.Scer\UAS.cBa} in the midgut cell progenitors under the control of Scer\GAL4^esg-NP5130 (limited to the adult stages using Scer\GAL80^ts.αTub84B) suppresses the formation of tumors in the anterior midgut of Sox21a⁶ mutant flies.

(Zhai et al., 2015)

Scer\GAL4^btl.PS-mediated expression of Egfr^{DN.Scer\UAS.cBa} suppresses the tracheal cyst phenotype of exp¹³⁵ embryos.

(Iordanou et al., 2014)

Expression of tay^{dsRNA.Scer\UAS} does not modify the wing vein loss phenotype seen when Egfr^{DN.Scer\UAS.cBa} is expressed under the control of Scer\GAL4^MD-638.

(Molnar and de Celis, 2013)

Neuronal Egfr^{DN.Scer\UAS.cBa} expression using Scer\GAL4^elav.PU suppresses the Cbl^GD12111-induced increase in body weight in males.

Expression of Egfr^{DN.Scer\UAS.cBa} in insulin producing cells under the control of Scer\GAL4^Ilp2.PR suppresses the Cbl^GD12111-induced increase in body weight in males.

(Yu et al., 2012)

The wing phenotypes resulting from the expression of Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^MD-638 are not modified by the co-expression of krz^{dsRNA.Scer\UAS}.

(Molnar et al., 2011)

Co-expression of Egfr^{DN.Scer\UAS.cBa} blocks the epithelium renewal induced by upd3^Scer\UAS.cMa-expression under the control of Scer\GAL4^esg-NP5130 and Scer\GAL80^ts.αTub84B.

Co-expression of Egfr^{DN.Scer\UAS.cBa} blocks the extra ISC proliferation induced by dome^{Scer\UAS.T:Avic\GFP-EGFP}-expression under the control of Scer\GAL4^esg-NP5130 and Scer\GAL80^ts.αTub84B.

(Buchon et al., 2010)

The dorsalised chorion phenotype caused by expression of Egfr::kek1^{KEΔCG.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^CY2 is completely suppressed by coexpression of Egfr^{DN.Scer\UAS.cBa}.

(Alvarado et al., 2004)

Expression of Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^ap-md544 partially suppresses the extra bristle phenotype seen in ed^1X5/ed^slH8 flies. The number of macrochaetae/heminotum in animals expressing Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^ap-md544 in a ed^1X5/ed^slH8 background is - anterior+posterior notopleural: 2.15, presutural: 1.05, anterior supraalar: 0.80, posterior supraalar: 0, anterior postalar: 0.60, posterior postalar: 0, anterior+posterior dorsocentral: 1.30 and anterior+posterior scutellar: 2.25.

(Escudero et al., 2003)

Co-expression of csw::Src64B^{src90.Scer\UAS} significantly suppresses the loss of VA2 muscle precursor cells seen in embryos expressing Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^twi.PG (71% of hemisegments have VA2 cells).

(Johnson Hamlet and Perkins, 2001)

The addition of stet^Scer\UAS.cGa or rho^Scer\UAS.cBa to Egfr^{DN.Scer\UAS.cBa}, Scer\GAL4^Bx-MS1096 flies gives a phenotype almost identical to that of Egfr^{DN.Scer\UAS.cBa}, Scer\GAL4^Bx-MS1096 flies alone.

(Guichard et al., 2000)

Co-expression of Egfr^{DN.Scer\UAS.cBa} and rho^Scer\UAS.cBa driven by Scer\GAL4^Bx-MS1096 in females has an almost identical phenotype to Scer\GAL4^Bx-MS1096 driven Egfr^{DN.Scer\UAS.cBa} expression alone. In males a stronger vein-loss phenotype is seen. This phenotype is not significantly affected by vn^Scer\UAS.cSa or S^Scer\UAS.cGb. Co-expression of Egfr^{DN.Scer\UAS.cBa}, Egfr^Scer\UAS.cBa and rho^Scer\UAS.cBa driven by Scer\GAL4^Bx-MS1096 fully restores the rho^Scer\UAS.cBa induced ectopic vein phenotype.

(Guichard et al., 1999)

The partial reduction in the development of muscles DA1 and VA2 seen in embryos expressing Egfr^{DN.Scer\UAS.cBa} under the control of both Scer\GAL4^twi.PG and Scer\GAL4^how-24B is suppressed by co-expression of S^Scer\UAS.cBa, and is dominantly enhanced by S^IIN.

(Buff et al., 1998)

The phenotypes caused by expression of Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^T155 are enhanced in a csw^LE120 or csw⁶ mutant background.

(Perkins et al., 1996)

The phenotypes caused by expression of Scer\GAL4^T155 under the control of Egfr^{DN.Scer\UAS.cBa} are enhanced in a csw^LE120 or csw⁶ mutant background.

(Perkins et al., 1996)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Egfr^DN.UAS.cBa is partially rescued by Scer\GAL4^twi.PG/Scer\GAL4^how-24B/Egfr^UAS.cBa

(Buff et al., 1998)

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Wild-type