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General Information
Symbol
Dmel\lbeUAS.cJa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Jagla
FlyBase ID
FBal0092633
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-lbe, UAS-Lb
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive lbe expression.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Mis-expression of lbeScer\UAS.cJa under the control of Scer\GAL4kirre.PR results in the formation of DA1, DT1 and VA2 having seven nuclei which is lower than that in wild-type.

Ectopic expression of lbeScer\UAS.cJa under the control of Scer\GAL4kirre.PR is sufficient to change the DA1 embryonic myoblast fusion rate to SBM-like one.

Myoblast driven expression of lbeScer\UAS.cJa (under the control of Scer\GAL41151) leads to severe defects in the patterning of dorsally located leg muscles. The tibia talm muscle is abnormally shaped and the homologous levator muscle in the femur is completely disrupted. A large proportion of dorsally located femur fibres normally contributing to the tilm muscle are unable to attach to the tilt tendon, most probably leading to its degeneration. Instead, they attach on both sides to the disc epithelium, adopting orientations perpendicular to the proximal-distal axis. In addition, some ectopic dorsal muscle fibres are observed in the proximal part of the tibia and femur.

Expression of lbeScer\UAS.cJa under the control of Scer\GAL41151 results in poor locomotor co-ordination and a spread-out positioning of the legs with respect to the body axis. The ability of these flies to catch, maintain and rotate a polystyrene ball are also impaired. About 60% of flies are unable to catch the ball and more than 80% lose it in less than 30 seconds, whereas wild-type flies do not have a problem with this.

When lbeScer\UAS.cJa is driven by Scer\GAL4l(3)31-1-31-1 defects in CNS morphology is seen. There is a thinning or absence of the posterior commissures in some neuromeres and a fusion of the commissures in others. The EL neurons deriving from NB 3-3 are also defective. Supernumerary subperineural and exit glial cells are also seen. The distance between the ISN and SN nerve clusters is decreased.

In lbeScer\UAS.cJa; Scer\GAL4tin.cBa, there are no obvious gain-of-function induced alterations in the patterning of the cardiac outflow region. In contrast, lbeScer\UAS.cJa; Scer\GAL4how-24B embryos exhibit abnormal contact of the cardiac outflow muscles with the tip of the heart. Most precisely, the cardiac outflow muscles attach directly to the most anterior cardiac cells that form the cardiac outflow and not to the second pair of cardiac cells as in the wild-type. In addition ventral bending of the most anterior part of the heart is more pronounced than in wild-type, and the cardiac outflow muscles themselves. However, the contact between heart anchoring cells and cardiac cells is not affected.

When lbeScer\UAS.cJa is driven by Scer\GAL4how-24B leads to a 60% loss in muscle DA1 founders and a 40% loss of DO1 founders.

Embryos expressing lbeScer\UAS.cJa under the control of Scer\GAL4how-24B have enlarged or duplicated segmental border muscles (SBMs) in approximately 70% of hemisegments. The number of lateral adult muscle precursors (LaPs) is significantly increased. The overproduction of SBM and LaPs is frequently associated with the loss of some neighbouring lateral muscle fibres. The number of ventral adult muscle precursors is significantly reduced.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of lbedsRNA.cMa and lbldsRNA.cMa under the control of Scer\GAL4how-24B results in 3 types of mutant phenotype in the segment border muscles (SBMs) of embryos. Approximately 37% of the embryos show a founder cell migration phenotype, such that the SBM founder is no longer located dorsally, leading to altered SBM shape and ventral accumulation of myoblast nuclei. 50% of the embryos show a myoblast fusion defect characterised by rounded cells close to the SBM. Approximately 13% of the embryos show abnormal attachment of the SBM to adjacent muscles and an abnormal SBM shape.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
lbeScer\UAS.cJa
lbeUAS.cJa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Jagla
Secondary FlyBase IDs
    References (14)