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General Information
Symbol
Dmel\sliUAS.cKa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Kidd
FlyBase ID
FBal0096688
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-slit, UAS-sli
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

sli is expressed under the control of UASt regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The growth cones of the aCC and RP2 neurons project normally in embryos expressing sliScer\UAS.cKa under the control of both Scer\GAL4arm.PS and Scer\GAL4Scer\UAS.cHa. The growth cone of the pCC neuron projects normally in embryos expressing sliScer\UAS.cKa under the control of both Scer\GAL4ptc-559.1 and Scer\GAL4Scer\UAS.cHa and no breaks in the longitudinal tracts are observed.

Expression of sliScer\UAS.cKa in the visceral mesoderm during salivary gland migration, driven by Scer\GAL4bap.3, causes glands to curve ventrally, away from the visceral mesoderm.

The brains of sliScer\UAS.cKa; Scer\GAL4loco.1.3 flies have ectopic glomeruli outside the normal antennal lobe circumference. This effect is more severe if multiple copies of sliScer\UAS.cKa are present.

Expression of sliScer\UAS.cKa under the control of Scer\GAL4C321c results in stalls and premature turns in 37% of the ganglionic branches. The longitudinal tracts in these embryos appear to form normally. Expression of sliScer\UAS.cKa under the control of Scer\GAL4en-e16E results in defects in 23% of the dorsal trunk tracheal branches. Expression of sliScer\UAS.cKa under the control of Scer\GAL4twi.PG results in visceral tracheal branches being generated from the T3 tracheal metamere in 11% of embryos (T3 does not normally produce visceral branches).

When expression is driven by Scer\GAL4elav.PLu, old embryos show defasciculation defects and subtle misroutings of longitudinal connectives across the midline. In earlier embryogenesis glial migration is delayed, defective migration along axons and increased glial cell numbers and increased distance from the midline between the connectives. More glia cluster at the site of exit of the axons from the CNS and some interface glia are missing from the connectives. Older embryos have recovered from these defects and look more normal.

sli2 embryos in which the midline has been rescued by expressing sliScer\UAS.cKa under the control of Scer\GAL4sim.P3.7 often show misinsertion of dorsal muscles 1,2, 9 and 10. However, the overall position of these muscles is not dramatically affected in these embryos. Muscles 21 to 23 are largely unaffected although occasionally an extra muscle is seen. 1% of segments show crossing of the midline by muscles 6/7, 54% show abnormal insertion of muscle 5 and 36% show abnormal insertion of muscles 6/7 (either failing to reach their specific muscle insertion sites in the epidermis - 12%, or reaching the epidermis but making abnormal connections - 24%). In sli2 embryos expressing sliScer\UAS.cKa under the control of Scer\GAL4en-e16E, 49% of segments show muscle cells aligned with sites of ectopic sli expression. In sli2 embryos expressing sliScer\UAS.cKa under the control of Scer\GAL4ptc-559.1, 20% of segments show muscle cells aligned with sites of ectopic sli expression.

The initial migration defect seen in the ventral muscle precursors of sli2 embryos is rescued by expression of sliScer\UAS.cKa under the control of Scer\GAL4sim.P3.7. However, striking defects are seen during the second phase, as muscles extend towards their (muscle attachment sites) MASs. Many muscles that normally attach at sli-positive MASs are instead attached to the wrong places in the epidermis. Muscles 6 and 7 either do not reach their MASs or make abnormal connections with the epidermis. Muscle 5 is often missing or is not properly attached at one or both ends. Muscle 4 is also often not properly attached. Expression of sliScer\UAS.cKa under the control of Scer\GAL4en-e16E results in little change in the muscle pattern. Expression of sliScer\UAS.cKa under the control of Scer\GAL4en-e16E in a sli2 background results in dramatic muscle patterning defects. Dorsal muscles 1, 2, 9 and 10 often fail to stretch across each segment and instead align themselves along the stripe of ectopic sli expression.

The central nervous system commissures are thicker and more fuzzy in embryos expressing sliScer\UAS.cKa under the control of Scer\GAL4elav.PLu or Scer\GAL4sca-537.4 than in wild-type embryos. The longitudinal tracts are thiner than normal, the innermost pCC axons aberrantly cross the midline, and in addition, the lateral and medial pathways are affected, also sometimes crossing the midline. When sliScer\UAS.cKa is expressed in the muscles using Scer\GAL4how-24B, most of the ISNb motor axons stall in the vicinity of their target muscles (6, 7, 12 and 13) and fail to innervate them. The morphology of the muscles is normal.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Suppressor of
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

23.8% of dMP2 neurons project contralaterally in sliGA20/robo1 transheterozygous embryos. This phenotype is suppressed by expression of sliScer\UAS.cKa under the control of Scer\GAL4605.

The induction of sliScer\UAS.cKa under the control of Scer\GAL4sim.PS in a ptcunspecified mutant background does not rescue the ptcunspecified phenotype.

Expression of sliScer\UAS.cKa under the control of the autoregulatory loop Scer\GAL4sim.PS and Scer\GAL4Scer\UAS.cHa in a ptcunspecified mutant background does not rescue the ptcunspecified phenotype.

Expression of sliScer\UAS.cKa under the control of Scer\GAL4twi.PG in a learobo2-8 background does not result in any new tracheal branches growing from trunk metameres T2 to T9. Expression of sliScer\UAS.cKa under the control of Scer\GAL4twi.PG in a robounspecified background results in the formation of additional visceral branches from metamere T3 and also from other tracheal metameres.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of sliScer\UAS.cKa under the control of Scer\GAL4Mef2.PR (which drives expression in all cardioblasts and somatic muscle cells before they begin their migration toward the dorsal midline) significantly rescues the sli2 mutant phenotype in 95% of cases. The cardioblasts adhere to each other and there are no visible gaps between adjacent cardioblasts and pericardial cells. Rescued embryos show a similar number of of Mef2-positive cardioblasts at the dorsal midline and columnar-shaped cardioblasts similar to wild type controls.

The vmd1a neuron of the ventral multidendritic neuron cluster can cross the midline in sli2 embryos. This phenotype is rescued by expression of sliScer\UAS.cKa under the control of Scer\GAL4sim.P3.7.

The photoreceptor axon targeting defects seen in slik04807b/sli2 third instar larvae are largely rescued by expression of sliScer\UAS.cKa under the control of Scer\GAL4bi-md653.

The midline is rescued in sli2 embryos expressing sliScer\UAS.cKa under the control of Scer\GAL4sim.P3.7.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
sliScer\UAS.cKa
sliUAS.cKa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Kidd
Secondary FlyBase IDs
    References (16)