FlyBase Allele Report: Dmel\E2f1[i2]

General Information

Symbol

Dmel\E2f1ⁱ²

Species

D. melanogaster

Name

FlyBase ID

FBal0097365

Feature type

allele

Associated gene

Dmel\E2f1

Associated Insertion(s)

Carried in Construct

Also Known As

dE2F1ⁱ²

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Moon et al., 2005)

Mutagen

ethyl methanesulfonate

(Royzman et al., 1999)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q527term.

(Royzman et al., 1999)

Nucleotide substitution: C?T.

(Royzman et al., 1999)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:21,623,523..21,623,523 [-]

Nucleotide change:

C21623523T

Reported nucleotide change:

C?T

Amino acid change:

Reported amino acid change:

Q527term

(Royzman et al., 1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell death

(Royzman et al., 2002)

abnormal size | oogenesis

(Royzman et al., 2002)

female semi-sterile | recessive

(Royzman et al., 1999)

female sterile

(Royzman et al., 2002, Du, 2000)

viable

(Du, 2000, Royzman et al., 1999)

viable (with E2f1⁹¹)

(Cayirlioglu et al., 2003)

visible, with E2f1^RNAi.UAS.cJa, Scer\GAL4^GMR.PU

(Ji et al., 2012)

visible | recessive

(Royzman et al., 1999)

Phenotype Manifest In

dorsal appendage (with E2f1⁰⁷¹⁷²)

(Geng and MacDonald, 2007)

egg (with E2f1⁰⁷¹⁷²)

(Geng and MacDonald, 2007)

egg chorion

(Royzman et al., 1999)

eye

(Royzman et al., 1999)

eye, with E2f1^RNAi.UAS.cJa, Scer\GAL4^GMR.PU

(Ji et al., 2012)

fertilized egg | maternal effect

(Royzman et al., 2002)

follicle cell

(Royzman et al., 1999)

heterochromatin & nurse cell (with Df(3R)e-BS2)

(Royzman et al., 2002)

nuclear chromosome & nurse cell (with Df(3R)e-BS2)

(Royzman et al., 2002)

nuclear membrane & nurse cell (with Df(3R)e-BS2)

(Royzman et al., 2002)

nurse cell

(Royzman et al., 2002)

nurse cell (with Df(3R)e-BS2)

(Royzman et al., 2002)

oocyte (with E2f1⁰⁷¹⁷²)

(Geng and MacDonald, 2007)

ovary (with Df(3R)e-BS2)

(Royzman et al., 2002)

polar body nucleus (with Df(2R)vg56)

(Royzman et al., 2002)

unfertilized egg

(Royzman et al., 2002)

wing disc | wandering third instar larval stage

(Moon et al., 2005)

Detailed Description

Statement

Reference

Expression of one copy of E2f1^RNAi.UAS.cJa under the control of Scer\GAL4^GMR.PU results in a slight rough eye phenotype. The phenotype is more severe if the flies also carry E2f1ⁱ²/+.

(Ji et al., 2012)

E2fⁱ²/E2f⁰⁷¹⁷² trans-heterozygotes are viable and can complete oogenesis. Eggs laid by these females display a variety of dorsoventral polarity defects, including eggshells with fused dorsal appendages (ventralised, 22%), eggshells with fused but slightly expanded dorsal appendages (between dorsalised and ventralised, 39%), and eggshells with fused but widely spread dorsal appendages (strongly dorsalised, 11%). In addition, 8% of the eggs laid by E2fⁱ²/E2f⁰⁷¹⁷² females have greatly degenerated dorsal appendages, which obscure the dorsoventral polarity pattern of these eggshells.

(Geng and MacDonald, 2007)

In wild-type animals, exposure to 40Gy of γ rays induces a distinct pattern of apoptosis in the wing disc, focussed on the the wing pouch, but excluding the DV boundary region and prospective vein. In E2fⁱ² homozygotes, the same irradiation treatment induces less cell death was reduced in the intervein regions but increased cell death at the D/V boundary.

(Moon et al., 2005)

The ovaries of E2fⁱ²/Df(3R)e-BS2 females are smaller than wild-type but have the normal complement of nurse and follicle cells. Occasionally, degenerating early egg chambers (stage 8 and earlier) are seen. The predominant defects in E2fⁱ²/Df(3R)e-BS2 females are a failure of nurse cells to deposit their contents into the oocyte and degenerate and an increased amount of heterochromatic DNA in the nurse cell nuclei. This appears to be caused by continued replication, when normally replication is blocked by a reduction in E2f activity. In E2fⁱ²/Df(3R)e-BS2 mutants defects in nurse cell chromosome morphology are also seen. Although mutant chromosomes go through the polytene and bulbous states, the homologous copies of each of the large chromosomes are frequently separated rather than associated as in wild-type. The fourth chromosome is always a single sphere. Sister chromatids often do not separate after the bulbous stage, becoming more condensed and having a polytene rather than interphase appearance. Individual chromosome arms are visible, and the chromosomes are clustered as if the homologous sister chromatids remain in proximity. In stage 12 E2fⁱ¹/Df(3R)e-BS2 egg chambers the nurse cell nuclear envelope does not break down as normal. In homozygous E2fⁱ² egg chambers, unlike wild-type (where nurse cells undergo apoptosis during stages 12 to 13 and have degenerated by stage 14), degeneration of nurse cells is abnormal and occurs late if at all. E2fⁱ²/Df(3R)e-BS2 mutant mothers lay few eggs. In the rare cases in which mature eggs are laid most are unfertilized, apparently due to defects in chorion structure. The few fertilized embryos arrest in early division cycles. In addition, the morphology of the polar bodies are affected. In embryos collected from these mothers some have normal polar body rosettes whereas others show aberrant morphology. Only a few eggs show some nuclear divisions, but with aberrant chromosome condensation.

(Royzman et al., 2002)

Hemizygotes are fully viable and have slightly rough eyes. Hemizygous females have reduced fertility. E2fⁱ¹/E2fⁱ² flies are fully viable and fertile. One copy of CycE⁰⁵²⁰⁶ has no effect on the viability of E2fⁱ²/E2f⁹¹ flies. E2fⁱ² flies produce thick and rough eggshells. The size and intensity of foci of BrdU incorporation are increased in the follicle cells of hemizygous egg chambers compared to wild type.

(Royzman et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

E2f1[+]/E2f1ⁱ² is a non-enhancer of decreased size | adult stage | somatic clone - tissue specific phenotype of hid^GMR.PG

(Herz and Bergmann, 2024)

E2f1[+]/E2f1ⁱ² is a non-enhancer of visible | adult stage | somatic clone - tissue specific phenotype of hid^GMR.PG

(Herz and Bergmann, 2024)

Suppressor of

Statement

Reference

E2f1[+]/E2f1ⁱ² is a suppressor of visible | adult stage | somatic clone - tissue specific phenotype of Kdm5^I1, hid^GMR.PG

(Herz and Bergmann, 2024)

E2f1[+]/E2f1ⁱ² is a suppressor of decreased size | adult stage | somatic clone - tissue specific phenotype of Kdm5^I1, hid^GMR.PG

(Herz and Bergmann, 2024)

E2f1ⁱ²/Scer\GAL4^ptc.PU is a suppressor of increased size | recessive | larval stage phenotype of lncRNA:DREAMer^KO

(Li et al., 2024)

E2f1ⁱ²/E2f1^rM729 is a suppressor of increased cell death | somatic clone | third instar larval stage phenotype of Rbf^15aΔ, Stam¹⁹

(Sheng et al., 2016)

E2f1ⁱ² is a suppressor of increased cell death phenotype of Rbf^15aΔ, gig⁶⁴

(Li et al., 2010)

E2f1ⁱ² is a suppressor of increased cell death phenotype of Rbf^15aΔ, gig¹⁹²

(Li et al., 2010)

NOT Suppressor of

Statement

Reference

E2f1[+]/E2f1ⁱ² is a non-suppressor of decreased size | adult stage | somatic clone - tissue specific phenotype of hid^GMR.PG

(Herz and Bergmann, 2024)

E2f1[+]/E2f1ⁱ² is a non-suppressor of visible | adult stage | somatic clone - tissue specific phenotype of hid^GMR.PG

(Herz and Bergmann, 2024)

Other

Statement

Reference

E2f1ⁱ²/E2f1⁹¹, E2f2^1-188, E2f2³²⁹ has lethal phenotype

(Cayirlioglu et al., 2003)

E2f1ⁱ²/E2f1^rM729, Rbf¹⁴ has partially lethal phenotype

(Du, 2000)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

E2f1[+]/E2f1ⁱ² is a non-enhancer of eye | somatic clone - tissue specific phenotype of hid^GMR.PG

(Herz and Bergmann, 2024)

Suppressor of

Statement

Reference

E2f1[+]/E2f1ⁱ² is a suppressor of eye | somatic clone - tissue specific phenotype of Kdm5^I1, hid^GMR.PG

(Herz and Bergmann, 2024)

E2f1ⁱ²/Scer\GAL4^ptc.PU is a suppressor of embryonic/larval salivary gland | larval stage phenotype of lncRNA:DREAMer^KO

(Li et al., 2024)

E2f1ⁱ²/Scer\GAL4^ptc.PU is a suppressor of nucleus | larval stage phenotype of lncRNA:DREAMer^KO

(Li et al., 2024)

E2f1ⁱ²/E2f1^rM729 is a suppressor of eye disc | somatic clone | third instar larval stage phenotype of Rbf^15aΔ, Stam¹⁹

(Sheng et al., 2016)

E2f1ⁱ² is a suppressor of eye | somatic clone phenotype of Rbf^15aΔ, gig⁶⁴

(Li et al., 2010)

E2f1ⁱ² is a suppressor of eye | somatic clone phenotype of Rbf^15aΔ, gig¹⁹²

(Li et al., 2010)

E2f1ⁱ²/E2f1^rM729 is a suppressor of eye | somatic clone phenotype of Rbf^15aΔ, rno¹

(Steele et al., 2009)

E2f1ⁱ²/E2f1^rM729 is a suppressor of photoreceptor cell R8 | somatic clone | third instar larval stage phenotype of Rbf^15aΔ, rno¹

(Steele et al., 2009)

NOT Suppressor of

Statement

Reference

E2f1[+]/E2f1ⁱ² is a non-suppressor of eye | somatic clone - tissue specific phenotype of hid^GMR.PG

(Herz and Bergmann, 2024)

Additional Comments

Genetic Interactions

Statement

Reference

The high levels of apoptosis (detected by Caspase-3 staining) observed in Rbf^15aΔ;Stam¹⁹ double homozygous somatic clones in third instar larval eye disc is completely abolished when the clones are created in E2f1[i2]/E2f1[rM729] mutant background.

(Sheng et al., 2016)

The presence of an E2fⁱ² background significantly decreases gig⁶⁴/Rbf^15aΔ induced cell death in the eye. In addition, much larger gig⁶⁴/Rbf^15aΔ double mutant clones are observed in adult eyes.

The presence of an E2fⁱ² background significantly decreases gig¹⁹²/Rbf^15aΔ induced cell death in the eye. In addition, much larger gig¹⁹²/Rbf^15aΔ double mutant clones are observed in adult eyes.

(Li et al., 2010)

The shiny-eye and multiple photoreceptor-R8 phenotype seen in rno¹, Rbf^15aΔ eye somatic mutant clones is significantly suppressed in a E2fⁱ²/E2f^rM729 mutant background.

(Steele et al., 2009)

Approximately 50% of the expected number of Rbf¹⁴/Y ; E2fⁱ²/E2f^rM729 flies survive to the adult stage, and the development of these flies is not significantly delayed compared to sibling flies. The surviving flies have normal eyes, normal macrochaetae on the notum and normal wings and legs.

(Du, 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

7274

e^* E2f1ⁱ²/TM3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

E2F1ⁱ²

(Herz and Bergmann, 2024, Manansala et al., 2013)

E2f1ⁱ²

(Cayirlioglu et al., 2003)

E2fⁱ²

dE2F1ⁱ²

(Steele et al., 2009, Royzman et al., 2002, Bosco et al., 2001)

dE2F<up>i2 </up>

(Rowland and Bernards, 2006)

dE2Fⁱ²

(Royzman et al., 1999, Royzman and Orr-Weaver, 1998)

dE2f1ⁱ²

(Ji et al., 2012)

de2f1ⁱ²

(Sheng et al., 2016, Li et al., 2010, Moon et al., 2005, Du, 2000)

e2f1ⁱ²

(Li et al., 2024, Li et al., 2021)

(Geng and MacDonald, 2007)

Name Synonyms

Secondary FlyBase IDs

References (18)

Report Sections

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