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General Information
Symbol
Dmel\spen5
Species
D. melanogaster
Name
FlyBase ID
FBal0104420
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Comment:

54bp deletion in the spen coding region.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Nucleotide deletion at 2L:192716-192769 .

Deletion of nucleotides 39548-39601. The resulting frameshift is predicted to cause premature termination of the protein.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Adulthood-generated spen5 midgut clones have significantly more cells and show a severe increase in the number of intestinal stem cells (Dl-positive, N-positive, Spdo-positive), but not enteroendocrine (Pros-positive) or enterocyte (polyploid) cells, as compared to control clones; these clones also show a severe increase in the proportion of mitotic cells, namely mitotic stem cells (Dl-positive), as compared to control clones.

spen5 clones do not show sensory organ differentiation defects in pupae.

Mutant embryos derived from females containing homozygous spen3 germline clones crossed to spen5/+ males (lacking both maternal and zygotic spen function) show alterations in the number of many peripheral and central nervous system cell types, and the development of other organs is affected. The number of lateral chordotonal organs in each abdominal hemisegment varies from 0 to 6, and is typically 4 (wild-type number is 5). Clusters containing the normal number are often disorganised. Mutant embryos derived from females containing homozygous spen5 germline clones crossed to spen3/+ males (lacking both maternal and zygotic spen function) show alterations in the number of many peripheral and central nervous system cell types, and the development of other organs is affected. The intersegmental nerve motor axon pathway and commissural central nervous system axon tracts are normal in homozygous, hemizygous or spen3/spen5 embryos. Defects are seen in the elongation and pathfinding of axons in the intersegmental nerve b (ISNb) and segmental nerve a (SNa) motor axon pathways and in the transverse nerve.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Suppressed by
NOT suppressed by
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (2)