FlyBase Allele Report: Dmel\capt[E636]

General Information

Symbol

Dmel\capt^E636

Species

D. melanogaster

Name

FlyBase ID

FBal0117577

Feature type

allele

Associated gene

Dmel\capt

Associated Insertion(s)

Carried in Construct

Also Known As

acu^E636

Genomic Maps

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Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Benlali et al., 2000)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

point mutation

2L:1,153,004..1,153,004 [-]

Nucleotide change:

G1153004A

Amino acid change:

W145term | capt-PA; W504term | capt-PB; W145term | capt-PC

Reported amino acid change:

W145term

Comment:

G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation. (exact site of mutation unspecified). Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Benlali et al., 2000)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Nature of the lesion

Statement

Reference

Amino acid replacement: W145term.

(Benlali et al., 2000)

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates muscular dystrophy

modeled by Dys^{dsRNA.NH2.UAS}

(Kucherenko et al., 2011)

modeled by Dg^O86

(Marrone et al., 2011)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage

(Medina et al., 2008)

abnormal neuroanatomy | larval stage (with capt^K304)

(Medina et al., 2008)

female sterile | germline clone | recessive

(Benlali et al., 2000)

lethal (with capt^K304)

(Medina et al., 2008)

lethal | recessive

(Benlali et al., 2000)

visible | recessive | somatic clone

(Benlali et al., 2000)

Phenotype Manifest In

dendrite | larval stage

(Medina et al., 2008)

dendrite | larval stage (with capt^K304)

(Medina et al., 2008)

dorsal-ventral compartment boundary of the wing disc | cell non-autonomous

(Major and Irvine, 2005)

egg | germline clone

(Benlali et al., 2000)

eye | somatic clone

(Benlali et al., 2000)

eye disc & actin filament | somatic clone

(Benlali et al., 2000)

eye disc | somatic clone

(Benlali et al., 2000)

morphogenetic furrow | somatic clone

(Benlali et al., 2000)

neuron & eye disc | posterior | somatic clone

(Benlali et al., 2000)

photoreceptor cell & eye disc | somatic clone

(Benlali et al., 2000)

wing margin bristle | somatic clone

(Benlali et al., 2000)

Detailed Description

Statement

Reference

Homozygous and capt^K304/capt^E636 larvae have punctate-like accumulations expressing Act5C^{Scer\UAS.T:Avic\GFP} in the dendrites of the sensory neuron dorsal cluster (these actin accumulations are absent in the dendrites of wild-type larvae).

(Medina et al., 2008)

Two days after their induction in the wing disc, capt^E636 clones cause strong disturbances of the dorsoventral boundary. The disruptions are bidirectional and non-autonomous in nature; ventral cells can appear in the dorsal part of the disc and dorsal cells can appear in the ventral part of the disc, and wild-type cells adjacent to clones are also affected. The anterior-posterior compartment boundary is not affected and there is no cell death or cell loss from the disc epithelium.

(Major and Irvine, 2005)

Homozygotes show lethality with a variable onset, from the embryonic to the second larval instar stage. Homozygous clones overlapping the morphogenetic furrow show premature photoreceptor differentiation. Many cells in posterior homozygous clones in the eye disc fail to differentiate as neurons and later appear to die, leaving scars in the adult eye. Eye discs in which all capt function has been removed (by inducing clones in a Minute background) show a severe disorganisation of the pattern of differentiation. Clones at the wing margin have wing margin bristles of abnormal shapes and sizes and the bristles are sometimes missing. Females containing homozygous germ line clones do not lay eggs. Homozygous clones in the eye disc show excessive actin polymerisation; actin filaments appear to fill the cell bodies of the photoreceptor cells, rather than being restricted to a small region of each ommatidium (as occurs in wild type). Mutant cells in the region of the morphogenetic furrow do not undergo the normal shape changes and instead retain large apical profiles. Apical constriction of cells at the lateral edges of mutant clones in the eye disc is seen.

(Benlali et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Csk^GD9345, Scer\GAL4^ptc-559.1, capt^E636/capt[+] has abnormal cell migration phenotype

(Rudrapatna et al., 2014)

Csk^GD9345, capt^E636 has lethal phenotype

(Rudrapatna et al., 2014)

Df(3R)Exel6184/+, capt^E636 has abnormal neuroanatomy | third instar larval stage phenotype

(Marrone et al., 2011)

Phenotype Manifest In

Enhancer of

Statement

Reference

capt^E636/capt[+] is an enhancer of indirect flight muscle phenotype of Dys^{dsRNA.NH2.UAS}, Scer\GAL4^Act.PU

(Kucherenko et al., 2011)

NOT Enhancer of

Statement

Reference

capt^E636/capt[+] is a non-enhancer of indirect flight muscle phenotype of Dg^dsRNA.UAS, Scer\GAL4^Act.PU

(Kucherenko et al., 2011)

Other

Statement

Reference

Csk^GD9345, Scer\GAL4^ptc-559.1, capt^E636/capt[+] has wing disc phenotype

(Rudrapatna et al., 2014)

Df(3R)Exel6184/+, capt^E636 has indirect flight muscle phenotype

(Kucherenko et al., 2011)

Df(3R)Exel6184/+, capt^E636 has lamina plexus | third instar larval stage phenotype

(Marrone et al., 2011)

Df(3R)Exel6184/+, capt^E636 has rhabdomere | adult stage phenotype

(Marrone et al., 2011)

Dg^O86, capt^E636/capt[+] has rhabdomere | adult stage phenotype

(Marrone et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

A capt^E636 heterozygous mutant background modifies the actin remodelling and subsequent basolateral invasion of epithelial cells seen in flies expressing Csk^GD9345 in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4^ptc-559.1.

(Rudrapatna et al., 2014)

capt Dys double heterozygous flies (capt^E636/Df(3R)Exel6184) exhibit indirect flight muscle degeneration.

capt^E636 Dg^O86 double heterozygous flies do not exhibit indirect flight muscle degeneration.

One copy of capt^E636 enhances the indirect flight muscle degeneration seen when Dys^{dsRNA.NH2.Scer\UAS} is expressed under the control of Scer\GAL4^Act.PU.

One copy of capt^E636 does not enhance the indirect flight muscle degeneration seen when Dg^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^tub.PU.

capt^E636 Dg³²³ double heterozygous flies do not exhibit indirect flight muscle degeneration.

capt^E636/+ mutant flies do not exhibit temperature-induced mobility defects.

(Kucherenko et al., 2011)

Combination of Df(3R)Exel6184 in heterozygous state with a single copy of capt^E636 results in a significantly increased frequency of lamina plexus defects in the third instar larval brain and reduced rhabdomere length in the adult eye in the double heterozygotes.

Combination of Dg^O86 in heterozygous state with a single copy of capt^E636 does not significantly affect the frequency of lamina plexus defects in the third instar larvae but results in a significantly reduced rhabdomere length in the adults.

(Marrone et al., 2011)

Central nervous system axons are seen to cross the midline in Abl² capt^E636 double heterozygous embryos. Expression of capt^Scer\UAS.cBa under the control of Scer\GAL4^elav.PLu completely rescues the midline crossing defects seen in capt^E636 Abl² double heterozygotes. Expression of capt^C.Scer\UAS under the control of Scer\GAL4^elav.PLu partially rescues the midline crossing defects seen in capt^E636 Abl² double heterozygotes.

(Wills et al., 2002)

Clones in the eye disc which are doubly mutant for capt^E636 and chic²²¹ show predominantly the chic²²¹ mutant phenotype.

(Benlali et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

capt^K304

(Medina et al., 2008)

Partially rescued by

capt^E636/capt^E593 is partially rescued by capt^C.UAS/Scer\GAL4^da.G32

(Benlali et al., 2000)

capt^E636/capt^E593 is partially rescued by Scer\GAL4^da.G32/capt^UAS.cBa

(Benlali et al., 2000)

Comments

Expression of capt^Scer\UAS.cBa under the control of Scer\GAL4^da.G32 rescues capt^E593/capt^E636 animals to viability, although the rescued adults sometimes show wing or eye defects. Expression of capt^C.Scer\UAS under the control of Scer\GAL4^da.G32 rescues capt^E593/capt^E636 animals to viability and restores normal photoreceptor differentiation.

(Benlali et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

5944

w^*; capt^E636 P{neoFRT}40A/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Capt^E636

(Marrone et al., 2011)

acu^E636

(Wills et al., 2002, Benlali et al., 2000)

capt^E636

(Nithianandam and Chien, 2018, Rudrapatna et al., 2014, Kucherenko et al., 2011, Medina et al., 2008, Janody and Treisman, 2006)

capt^W145

(Medina et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (9)

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