FlyBase Allele Report: Dmel\sqh[E20.E21]

General Information

Symbol

Dmel\sqh^E20.E21

Species

D. melanogaster

Name

FlyBase ID

FBal0122886

Feature type

allele

Associated gene

Dmel\sqh

Associated Insertion(s)

Carried in Construct

P{sqh-E20E21}

Also Known As

sqhEE, sqh^E20E21, sqhE20E21, sqh-E20E21

Key Links

Transgenic product class

missense_variant

(Winter et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Winter et al., 2001)

Mutagen

in vitro construct

(Winter et al., 2001)

Progenitor genotype

Carried in construct

P{sqh-E20E21}

Cytology

Description

A mutant form of sqh is expressed under the control of its own promoter; carries the amino acid replacements T20E and S21E.

(Winter et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

sqh

Encoded product / tool

sqh

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates cancer

modeled by M6^W186stop, Ras85D^V12.cUa.UAS

(Dunn et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

egg

(Ferreira et al., 2014)

egg chamber

(Ferreira et al., 2014)

nurse cell

(Ferreira et al., 2014)

Detailed Description

Statement

Reference

sqh^E20.E21/+ significantly reduces egg length, shows increased nurse cell-to-oocyte membrane collapse and high abnormal accumulation of F-actin in nurse cells of stage 9/10 egg chambers compared to controls.

(Ferreira et al., 2014)

Expression of sqh^E20.E21 rescues the para-segmental boundary defects found in Y-27632-treated embryos.

(Monier et al., 2010)

Expression of one copy of the sqh^E20.E21 transgene has no effect on axial expansion in wild-type flies.

(Chodagam et al., 2005)

The addition of sqh^E20.E21 rescues the germ band extension defects seen in embryos injected with Y-27632 (a specific inhibitor of rok).

(Bertet et al., 2004)

One copy of sqh^E20.E21 in a wild-type background has no effect on eye development.

(Lee and Treisman, 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

aurA^IM, sqh^E20.E21 has some die during pharate adult stage phenotype, suppressible by rok[+]/Rok²

(Moon and Matsuzaki, 2013)

aurA^IM, sqh^E20.E21 has some die during pharate adult stage phenotype, suppressible by zip[+]/zip¹

(Moon and Matsuzaki, 2013)

Enhancer of

Statement

Reference

sqh^E20.E21 is an enhancer of lethal - all die during P-stage phenotype of aurA^ST

(Moon and Matsuzaki, 2013)

sqh^E20.E21 is an enhancer of some die during pharate adult stage phenotype of aurA^IM

(Moon and Matsuzaki, 2013)

sqh^E20.E21 is an enhancer of increased mortality during development phenotype of aurA^WK

(Moon and Matsuzaki, 2013)

sqh^E20.E21 is an enhancer of abnormal neuroanatomy | third instar larval stage phenotype of asp^E3

(Rujano et al., 2013)

sqh^E20.E21 is an enhancer of visible phenotype of MYPT-75D^F117A.UAS, Scer\GAL4^Bx-MS1096

(Vereshchagina et al., 2004)

sqh^E20.E21 is an enhancer of abnormal neuroanatomy phenotype of Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng

(Kim et al., 2002)

sqh^E20.E21 is an enhancer of abnormal neuroanatomy phenotype of RhoGAPp190^RNAi.GAP.UAS, Scer\GAL4^ey-OK107

(Billuart et al., 2001)

sqh^E20.E21 is an enhancer of visible | recessive phenotype of ck⁰⁷¹³⁰

(Winter et al., 2001)

Suppressor of

Statement

Reference

sqh^E20.E21 is a suppressor of visible phenotype of Drak^KO

(Neubueser and Hipfner, 2010)

sqh^E20.E21 is a suppressor of lethal phenotype of Drak^del/Drak^KO, rok[+]/Rok²

(Neubueser and Hipfner, 2010)

sqh^E20.E21 is a suppressor of visible phenotype of flw¹, puc^A251.1F3/puc[+]

(Kirchner et al., 2007)

sqh^E20.E21 is a suppressor of abnormal cell polarity phenotype of Lkb1^X5

(Lee et al., 2007)

sqh^E20.E21 is a suppressor of abnormal mitotic cell cycle phenotype of Lkb1^X5

(Lee et al., 2007)

sqh^E20.E21 is a suppressor | partially of abnormal mitotic cell cycle | larval stage phenotype of AMPKα^D1

(Lee et al., 2007)

sqh^E20.E21 is a suppressor of visible | recessive phenotype of dsh¹

(Winter et al., 2001)

sqh^E20.E21 is a suppressor of lethal | recessive | larval stage phenotype of Rok²

(Winter et al., 2001)

NOT Suppressor of

Statement

Reference

sqh^E20.E21 is a non-suppressor of lethal | male phenotype of flw⁶

(Vereshchagina et al., 2004)

sqh^E20.E21 is a non-suppressor of lethal | male phenotype of flw^G0172

(Vereshchagina et al., 2004)

sqh^E20.E21 is a non-suppressor of abnormal planar polarity phenotype of dsh¹

(Winter et al., 2001)

Other

Statement

Reference

flw¹, sqh^E20.E21 has lethal | male phenotype

(Vereshchagina et al., 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

sqh^E20.E21 has egg phenotype, enhanceable by Pkn^1T/Pkn^1T

(Ferreira et al., 2014)

sqh^E20.E21 has egg phenotype, enhanceable by Pkn^2T/Pkn^2T

(Ferreira et al., 2014)

Enhancer of

Statement

Reference

sqh^E20.E21/sqh[+] is an enhancer of egg | germline clone phenotype of Pkn^1T

(Ferreira et al., 2014)

sqh^E20.E21/sqh[+] is an enhancer of egg | germline clone phenotype of Pkn^2T

(Ferreira et al., 2014)

sqh^E20.E21 is an enhancer of larval brain | third instar larval stage phenotype of asp^E3

(Rujano et al., 2013)

sqh^E20.E21 is an enhancer of wing phenotype of MYPT-75D^F117A.UAS, Scer\GAL4^Bx-MS1096

(Vereshchagina et al., 2004)

sqh^E20.E21 is an enhancer of eye phenotype of Rac1^GMR.PN

(Tan et al., 2003)

sqh^E20.E21 is an enhancer of larval longitudinal connective phenotype of Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng

(Kim et al., 2002)

sqh^E20.E21 is an enhancer of mushroom body vertical lobe phenotype of RhoGAPp190^RNAi.GAP.UAS, Scer\GAL4^ey-OK107

(Billuart et al., 2001)

sqh^E20.E21 is an enhancer of mushroom body medial lobe phenotype of RhoGAPp190^RNAi.GAP.UAS, Scer\GAL4^ey-OK107

(Billuart et al., 2001)

sqh^E20.E21 is an enhancer of wing hair | increased number phenotype of ck⁰⁷¹³⁰

(Winter et al., 2001)

Suppressor of

Statement

Reference

sqh^E20.E21 is a suppressor of wing phenotype of Drak^KO

(Neubueser and Hipfner, 2010)

sqh^E20.E21 is a suppressor of metathoracic leg phenotype of Drak^KO

(Neubueser and Hipfner, 2010)

sqh^E20.E21 is a suppressor of wing phenotype of flw¹, puc^A251.1F3/puc[+]

(Kirchner et al., 2007)

sqh^E20.E21 is a suppressor | partially of embryonic/first instar larval cuticle | maternal effect phenotype of AMPKα^D1

(Lee et al., 2007)

sqh^E20.E21 is a suppressor of cleavage nucleus phenotype of Cp190²

(Chodagam et al., 2005)

sqh^E20.E21 is a suppressor of eye phenotype of Scer\GAL4^ey.PB, sti^RNAi.Sym.UAS

(D'Avino et al., 2004)

sqh^E20.E21 is a suppressor of ommatidium phenotype of Scer\GAL4^ey.PB, sti^RNAi.Sym.UAS

(D'Avino et al., 2004)

sqh^E20.E21 is a suppressor of wing hair | increased number phenotype of dsh¹

(Winter et al., 2001)

sqh^E20.E21 is a suppressor of wing hair | increased number phenotype of Rok²

(Winter et al., 2001)

NOT Suppressor of

Statement

Reference

sqh^E20.E21 is a non-suppressor of mesoderm phenotype of stumps^09904b

(Wilson et al., 2005)

sqh^E20.E21 is a non-suppressor of wing hair phenotype of dsh¹

(Winter et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Shorter egg length in sqh^E20.E21/+ is enhanced in Pkn^1T/Pkn^1T or Pkn^2T/Pkn^2T germline clones.

(Ferreira et al., 2014)

Introducing one copy of sqh^E20.E21 into aur^ST, aur^IM, or aur^WK mutants significantly enhances aur mutant lethality.

A rok²/+ heterozygous background suppresses the enhanced lethality of aur^IM females bearing one copy of sqh^E20.E21, and ameliorates the enhanced rough eye phenotype of these flies.

A rok²/+ heterozygous background suppresses the enhanced lethality of aur^WK females bearing one copy of sqh^E20.E21, and ameliorates the enhanced rough eye phenotype of these flies.

(Moon and Matsuzaki, 2013)

sqh^E20.E21 enhances the neuroepithelial defects of asp^E3 larval brains.

(Rujano et al., 2013)

Expression of sqh^E20.E21 suppresses the short wing and deformed T3 leg phenotypes of Drak^KO mutants.

(Neubueser and Hipfner, 2010)

The wing defects seen in flw¹/Y ; puc^A251.1F3/+ flies are suppressed to wild type by sqh^E20.E21.

(Kirchner et al., 2007)

The epithelial polarity defects and genomic polyploidy defects seen in lkb1^X5 larvae are suppressed by sqh^E20.E21.

The cuticle defects seen in mutant embryos derived from SNF1A^D1 homozygous female germline clones are partially suppressed by sqh^E20.E21; the fraction of embryos that form cuticle is increased from approximately 10% to approximately 30%.

The fraction of SNF1A^D1 mitotic larval brain neuroblasts that show extreme polyploidy is decreased if the larvae are also mutant for sqh^E20.E21.

(Lee et al., 2007)

Expression of one copy of the sqh^E20.E21 transgene partially restores axial expansion in Cp190² germ-line clone embryos.

(Chodagam et al., 2005)

A single copy of the sqh^E20.E21 transgene is able to considerably suppress the sti^{dsRNA.Sym.Scer\UAS} (under the regulation of Scer\GAL4^ey.PB) rough eye phenotype.

(D'Avino et al., 2004)

The amount of Mbs^T541 photoreceptor clones that are mislocalized to the basal region of the eye disc is increased when these discs carry one copy of sqh^E20.E21 (a transgene that contains two phosphomimetic mutations).

(Lee and Treisman, 2004)

The flw¹ mutation is lethal in combination with the phosphomimetic sqh^E20.E21 mutation. The amount of blistering and crumpling of wings in flies expressing MYPT-75D^{F117A.Scer\UAS} under the control of Scer\GAL4^Bx-MS1096 is enhanced by sqh^E20.E21.

(Vereshchagina et al., 2004)

The small, rough eye phenotype of Rac1^GMR.PN flies is enhanced by heterozygosity for sqh^E20.E21.

(Tan et al., 2003)

4% of rok²/Y ; sqh^E20.E21/+ flies survive to adulthood. The multiple wing hair phenotype caused by rok² is almost completely suppressed by sqh^E20.E21. The dsh¹ multiple hair phenotype but not the hair misorientation phenotype is suppressed by sqh^E20.E21.

(Winter et al., 2001)

Xenogenetic Interactions

Statement

Reference

The midline crossover phenotype caused expression of Ggal\MLCK^ct.Scer\UAS under the control of Scer\GAL4^ftz.ng is enhanced by sqh^E20.E21.

(Kim et al., 2002)

Complementation and Rescue Data

Partially rescues

sqh^E20.E21 partially rescues sqh¹

(Royou et al., 2002)

sqh^E20.E21 partially rescues sqh^AX3

(Royou et al., 2002)

Comments

Expressing one copy of the sqh^E20.E21 transgene restores axial expansion in sqh¹ germline clones and substantially restores both oogenesis and subsequent axial expansion in sqh^AX3 germline clones.

(Royou et al., 2002)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

MRLC^E20E21

(Monier et al., 2010)

MRLC^EE

(Lee et al., 2007)

Sqh^E20/E21

(Neubueser and Hipfner, 2010)

Sqh^E20E21

(Kakanj et al., 2016)

Sqh^EE

(Neubueser and Hipfner, 2010)

sqh^E20.E21

sqh^E20E21

(D'Avino et al., 2004)

sqhE20E21

(Bertet et al., 2009, Barros et al., 2003)

Name Synonyms

Secondary FlyBase IDs

References (26)

Report Sections

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