FB2025_01 , released February 20, 2025
Allele: Dmel\Ras85DG12V.sev
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General Information
Symbol
Dmel\Ras85DG12V.sev
Species
D. melanogaster
Name
FlyBase ID
FBal0123114
Feature type
allele
Associated gene
Dmel\Ras85D
Associated Insertion(s)
Carried in Construct
P{sev-Ras85D.G12V}
Key Links
Transgenic product class
missense_variant
(Halfar et al., 2001)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Halfar et al., 2001)
Mutagen
in vitro construct
(Halfar et al., 2001)
Progenitor genotype
Carried in construct
P{sev-Ras85D.G12V}
Cytology
Description

Construct: Amino acid replacement: G12V. sev enhancer sequences drive expression of a mutated form of Ras85D.

(Halfar et al., 2001)
Allele components
Component
Use(s)
Regulatory region(s)
sev
Encoded product / tool
Ras85D
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Ras85DG12V.sev flies have a rough eye phenotype, with a significant increase in the number of R cells per ommatidium and ommatidia are often fused with neighbours.

      (Aron et al., 2010)

      Flies expressing Ras85DG12V.sev have rough eyes, due to the presence of additional R7 photoreceptor cells.

      (Halfar et al., 2001)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Suppressed by
      Statement
      Reference

      Ras85DG12V.sev, Scer\GAL4GMR.PU has visible phenotype, suppressible by dj-1βΔ93/dj-1beta[+]/DJ-1alpha[+]/DJ-1αΔ72

      (Aron et al., 2010)

      Ras85DG12V.sev has increased cell number | adult stage phenotype, suppressible by dj-1βΔ93/dj-1beta[+]/DJ-1alpha[+]/DJ-1αΔ72

      (Aron et al., 2010)
      Phenotype Manifest In
      Enhanced by
      Suppressed by
      Statement
      Reference

      Ras85DG12V.sev has eye phenotype, suppressible by dj-1βΔ93/dj-1beta[+]/DJ-1alpha[+]/DJ-1αΔ72

      (Aron et al., 2010)

      Ras85DG12V.sev has eye photoreceptor cell | increased number | adult stage phenotype, suppressible by dj-1βΔ93/dj-1beta[+]/DJ-1alpha[+]/DJ-1αΔ72

      (Aron et al., 2010)

      Ras85DG12V.sev has ommatidium phenotype, suppressible by dj-1βΔ93/dj-1beta[+]/DJ-1alpha[+]/DJ-1αΔ72

      (Aron et al., 2010)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      dj-1βΔ93/+ DJ-1αΔ72/+ suppresses eye phenotypes (including increase in R cell number per ommatidium) in Ras85DG12V.sev flies. Expression of DJ-1αScer\UAS.cMa driven by Scer\GAL4GMR.PU significantly enhances eye phenotypes (including increasing R cell number per ommatidium) in Ras85DG12V.sev flies.

      (Aron et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Ras85DG12V.sev
      Name Synonyms
      Secondary FlyBase IDs
        References (2)