FlyBase Allele Report: Dmel\Pvr[UAS.cDa]

General Information

Symbol

Dmel\Pvr^UAS.cDa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Duchek

FlyBase ID

FBal0127281

Feature type

allele

Associated gene

Dmel\Pvr

Associated Insertion(s)

Carried in Construct

P{UAS-Pvr.D}

Also Known As

UAS-Pvr, UASpvr

Key Links

Transgenic product class

cDNA

(Duchek et al., 2001)

wild_type

(Duchek et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Duchek et al., 2001)

wild_type

(Duchek et al., 2001)

Mutagen

in vitro construct

(Duchek et al., 2001)

Progenitor genotype

Carried in construct

P{UAS-Pvr.D}

Cytology

Description

UASt sequences drive expression of a full-length Pvr cDNA.

(Duchek et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Pvr

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates acute myeloid leukemia

modeled by Hsap\HOXA9::Hsap\NUP98^WT.UAS

(Baril et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

actin filament | ectopic, with Scer\GAL4^Act5C.PP

(Somogyi and Rorth, 2004)

adult midgut, with Scer\GAL4^NP5130

(Choi et al., 2008)

border follicle cell, with Scer\GAL4^slbo.2.6

(Poukkula et al., 2011)

cortical actin cytoskeleton & oocyte associated follicle cell, with Scer\GAL4^slbo.2.6

(Jekely et al., 2005)

cortical zone of lymph gland primary lobe | third instar larval stage, with Scer\GAL4^Hml.Δ

(Baril et al., 2017)

embryonic/larval hemocyte | increased number | larval stage, with Scer\GAL4^Hml.Δ

(P et al., 2020)

embryonic/larval hemocyte | third instar larval stage, with Scer\GAL4^Hml.Δ

(Baril et al., 2017)

enterocyte, with Scer\GAL4^NP5130

(Choi et al., 2008)

enteroendocrine cell | increased number, with Scer\GAL4^NP5130

(Choi et al., 2008)

intestinal stem cell, with Scer\GAL4^NP5130

(Choi et al., 2008)

prothoracic gland | larval stage, with Scer\GAL4^spok.1.45

(Pan and O'Connor, 2021)

wing, with Scer\GAL4^Hml.Δ

(P et al., 2020)

Detailed Description

Statement

Reference

Larval lymph glands expressing Pvr^UAS.cDa under the control of Scer\GAL4^Hml.Δ have a smaller cortical zone and fewer Hml-positive hemocytes than controls.

(Baril et al., 2017)

Scer\GAL4^He.PZ-mediated expression of Pvr^Scer\UAS.cDa does not affect hemocyte counts in larvae.

(Tran et al., 2013)

The late phase of posterior border follicle cell migration are transformed to a behaviour similar to that of the early phase in females expressing Pvr^Scer\UAS.cDa under the control of Scer\GAL4^slbo.2.6, with the clusters showing sliding movement, reduced tumbling and elongated shapes. The number of cellular extensions from the front of the clusters is also increased in the late phase of posterior border follicle cell migration in these animals. In contrast to wild type, the mutant border cell clusters fail to migrate dorsally upon reaching the oocyte.

(Poukkula et al., 2011)

The number of proliferating cells in the adult midgut is significantly increased in flies expressing Pvr^Scer\UAS.cDa under the control of Scer\GAL4^esg-NP5130 relative to controls.

The intestinal stem cell population in the midgut of flies expressing Pvr^Scer\UAS.cDa under the control of Scer\GAL4^esg-NP5130 is increased compared to controls. These flies show a 1.7 fold increase in the number of enteroendocrine cells and a 0.6 fold reduction in the number of differentiated enterocytes with large nuclei compared to controls.

(Choi et al., 2008)

Cells expressing Pvr^Scer\UAS.cDa under the control of Scer\GAL4^c522 are preferentially located at the front of the cluster during border cell posterior migration in mosaic border cell clusters consisting of both wild-type and mutant cells.

(Bianco et al., 2007)

Border cell migration occurs normally in Pvr^Scer\UAS.cDa; Scer\GAL4^slbo.2.6 egg chambers. By oogenesis stage 10, sporadic follicle cells in these egg chambers accumulate filamentous actin in the cortex.

(Jekely et al., 2005)

When Pvr^Scer\UAS.cDa is driven by Scer\GAL4^Act5C.PP in somatic clones in the follicle cells, an small increase is seen in the accumulation of F-actin.

(Somogyi and Rorth, 2004)

Expression of Pvr^Scer\UAS.cDa when driven by Scer\GAL4^slbo.2.6 has a negligible effect on border cell migration.

(Duchek et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^spok.1.45/Pvr^UAS.cDa is a suppressor of abnormal developmental rate phenotype of Ptth^120F2A

(Pan and O'Connor, 2021)

Pvr^UAS.cDa, Scer\GAL4^Hml.Δ is a suppressor of abnormal size | third instar larval stage phenotype of Hsap\HOXA9::Hsap\NUP98^WT.UAS, Scer\GAL4^Hml.Δ

(Baril et al., 2017)

Pvr^UAS.cDa, Scer\GAL4^Hml.Δ is a suppressor of increased cell number | third instar larval stage phenotype of Hsap\HOXA9::Hsap\NUP98^WT.UAS, Scer\GAL4^Hml.Δ

(Baril et al., 2017)

Other

Statement

Reference

Ptth^120F2A, Pvr^UAS.cDa, Scer\GAL4^spok.1.45 has decreased body size | P-stage phenotype

(Pan and O'Connor, 2021)

Phenotype Manifest In

Enhancer of

Statement

Reference

Scer\GAL4^slbo.2.6/Pvr^UAS.cDa is an enhancer of border follicle cell | somatic clone phenotype of Cbl^F165

(Jekely et al., 2005)

Pvr^UAS.cDa, Scer\GAL4^slbo.2.6 is an enhancer of border follicle cell phenotype of Pvf1^EPg11235, Scer\GAL4^slbo.2.6

(Duchek et al., 2001)

Suppressor of

Statement

Reference

Pvr^UAS.cDa, Scer\GAL4^Hml.Δ is a suppressor of embryonic/larval lymph gland | third instar larval stage phenotype of Hsap\HOXA9::Hsap\NUP98^WT.UAS, Scer\GAL4^Hml.Δ

(Baril et al., 2017)

Pvr^UAS.cDa, Scer\GAL4^Hml.Δ is a suppressor of embryonic/larval hemocyte | third instar larval stage phenotype of Hsap\HOXA9::Hsap\NUP98^WT.UAS, Scer\GAL4^Hml.Δ

(Baril et al., 2017)

Pvr^UAS.cDa, Scer\GAL4^Hml.Δ is a suppressor of posterior signaling center of lymph gland primary lobe | third instar larval stage phenotype of Hsap\HOXA9::Hsap\NUP98^WT.UAS, Scer\GAL4^Hml.Δ

(Baril et al., 2017)

Pvr^UAS.cDa, Scer\GAL4^Hml.Δ is a suppressor of posterior signaling center of lymph gland primary lobe | third instar larval stage phenotype of Arf1^GD12522, Scer\GAL4^Hml.Δ

(Khadilkar et al., 2014)

Pvr^UAS.cDa, Scer\GAL4^Hml.Δ is a suppressor of embryonic/larval lymph gland phenotype of Scer\GAL4^Hml.Δ, bip1^NIG.7574R

(Mondal et al., 2014)

Pvr^UAS.cDa, Scer\GAL4^slbo.2.6 is a suppressor of border follicle cell phenotype of Scer\GAL4^slbo.2.6, grk^ΔTC.UAS

(Poukkula et al., 2011)

Pvr^UAS.cDa, Scer\GAL4^slbo.2.6 is a suppressor of border follicle cell phenotype of Scer\GAL4^slbo.2.6, vn^EPg35521

(Duchek et al., 2001)

NOT Suppressor of

Statement

Reference

Pvr^UAS.cDa, Scer\GAL4^Hml.Δ is a non-suppressor of embryonic/larval plasmatocyte | third instar larval stage phenotype of Arf1^GD12522, Scer\GAL4^Hml.Δ

(Khadilkar et al., 2014)

Pvr^UAS.cDa, Scer\GAL4^Hml.Δ is a non-suppressor of embryonic/larval crystal cell | third instar larval stage phenotype of Arf1^GD12522, Scer\GAL4^Hml.Δ

(Khadilkar et al., 2014)

Other

Statement

Reference

Pvr^UAS.cDa, Scer\GAL4^slbo.2.6, spri^6G1 has border follicle cell phenotype

(Jekely et al., 2005)

Pvf1^UASp.cRb, Pvr^UAS.cDa, Scer\GAL4^slbo.2.6 has border follicle cell phenotype

(Jekely et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Pvr^Scer\UAS.cDa does not suppress the increase in the differentiation of prohemocytes to plasmatocytes and crystal cells seen when Arf79F^GD12522 is expressed under the control of Scer\GAL4^Hml.Δ. The moderate reduction in niche (posterior signalling center) cell number is suppressed upon expression of Pvr^Scer\UAS.cDa.

(Khadilkar et al., 2014)

Expression of Pvr^Scer\UAS.cDa under the control of Scer\GAL4^Act.PU in the cells surrounding scrib¹ clones in the eye-antennal disc significantly enhances the elimination of the mutant scrib¹ clones compared to that seen when the cells surrounding the clone are wild type.

(Ohsawa et al., 2011)

Defective migration of border follicle cells in Cbl^F165 homozygous somatic clones is enhanced by Pvr^Scer\UAS.cDa with Scer\GAL4^slbo.2.6.

Border follicle cell migration is frequently defective in Pvf1^{Scer\UAS.P\T.cRb}; Pvr^Scer\UAS.cDa; Scer\GAL4^slbo.2.6 animals.

Migration of border follicle cells is normal in spri^6G1 homozygotes, or in flies carrying Pvr^Scer\UAS.cDa with Scer\GAL4^slbo.2.6, but significant defects in border cell migration border cell migration defects are seen in spri^6G1/spri^6G1 Pvr^Scer\UAS.cDa Scer\GAL4^slbo.2.6 flies.

(Jekely et al., 2005)

When Pvr^Scer\UAS.cDa is added to Pvf1^EPg11235, Scer\GAL4^slbo.2.6 an enhancement of the border cell migration phenotype is seen. A quarter of the border cells at stage 10 are at the oocyte, but another quarter do not move at all.

(Duchek et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

58998

w^*; P{UAS-Pvr.D}3

58429

w¹¹¹⁸; P{UAS-Pvr.D}8

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Pvr^Scer\UAS.cDa

Pvr^UAS.cDa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Duchek

(Duchek et al., 2001)

Secondary FlyBase IDs

References (20)

Report Sections

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