Open Close
General Information
Symbol
Dmel\jeb-c1
Species
D. melanogaster
Name
FlyBase ID
FBal0127957
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Excision of the P{lacW} element.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Homozygous jeb-c1 mutant longitudinal visceral muscle (LVM) founder cells in stage 12 embryos migrate normally towards the trunk visceral mesoderm (TVM) and very few dying cells are seen. At stage 13 the front migrating cells reach the anterior end of the trunk visceral mesoderm as in wild type. However during late stage 13 the migration becomes disordered and progressive founder cell death is seen.

jeb-c1 mutants show a salivary gland phenotype. At stage 14, mutant salivary glands remain associated with the inner circular muscle layer, while in wild type, these structures become separate. After stage 15, cells from the distal tips of the jeb-c1 salivary glands spread into the region of the undifferentiated midgut that forms the gastric caecae in the wild-type embryos. The mutant glands become mispositioned and/or elongated and maintain contact with the area of the midgut immediately adjacent to the proventriculus.

Marker analysis indicates that no visceral muscle founders are specified in jeb-c1 mutant embryos. Myoblasts do form in the visceral mesoderm of these embryos, but these cells fuse with somatic muscle founders (i.e.- they are still fusion competent). The resulting animals exhibit loss of visceral musculature, but have normal somatic muscle patterning.

Differentiated visceral mesoderm is not seen in mutant embryos, although other mesodermal tissues (somatic muscles, heart, fat body and hemocytes) develop normally. Visceral mesoderm precursor cells are specified but fail to migrate normally. There is an increase in the number of nuclei in positions consistent with an increase in somatic muscle precursors, but there is no major disruption of somatic muscle patterning. The midgut endoderm is specified normally and migrates to form two longitudinal bands. Subsequent dorsal and ventral endoderm migration is abnormal (probably because this migration depends on the visceral mesoderm).

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Statement
Reference

jeb-c1 has muscle founder cell & visceral mesoderm phenotype, suppressible by Scer\GAL4bap.3/Alk::Hsap\NPM1UAS.act

Additional Comments
Genetic Interactions
Statement
Reference

Ras85DV12.Scer\UAS; Scer\GAL4twi.2PE substantially suppresses the visceral mesoderm phenotype seen in stage 12 jeb-c1 homozygous embryos. Loss of visceral muscle founder cells in jeb-c1 mutant embryos, is suppressed by AlkScer\UAS.T:Hsap\NPM1; Scer\GAL4bap.3.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (5)