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General Information
Symbol
Dmel\RhoGAPp190dsRNA.N.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0128099
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of two copies of RhoGAPp190 sequence (the N-terminal domain), arranged in an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of RhoGAPp190dsRNA.N.Scer\UAS under the control of Scer\GAL4elav.PLu results in premature defasciculation of ISNb axons prior to reaching muscle 13, and sometimes muscle 6, reflecting either increased defasciculation or a defect in muscle target recognition. The ISNb premature branching phenotype is seen in 22.1% of hemisegments, while the total fraction of hemisegments showing ISNb defects is 36.4%.

Expression of RhoGAPp190dsRNA.N.Scer\UAS under the control of Scer\GAL4OK107 results in truncation or loss of dorsal branches of the mushroom body.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Sema-1ak13702/+ suppresses the premature ISNb branching seen in embryos expressing RhoGAPp190dsRNA.N.Scer\UAS under the control of Scer\GAL4elav.PLu from 22.1% to 8.2% of hemisegments, while the total fraction of hemisegments showing ISNb defects is reduced from 36.4% to 21.2% in these animals.

Heterozygosity for either Df(4)C3 or plexBKG00878 has no effect on the mutant ISNb phenotype seen in embryos expressing RhoGAPp190dsRNA.N.Scer\UAS under the control of Scer\GAL4elav.PLu.

pbl2/+ significantly reduces the penetrance of the ISNb premature branching phenotype seen in embryos expressing RhoGAPp190dsRNA.N.Scer\UAS under the control of Scer\GAL4elav.PLu (from 22.1% to 9.4% of hemisegments). These embryos show a significant increase in defasciculation defects (excluding premature branching defects) at the last ISNb choice point.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

Transcription from the P{UAS-RhoGAPp190.N-dsRNA} construct should produce RhoGAPp190 dsRNA, resulting in dsRNA interference (RNAi) of the RhoGAPp190 gene.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
RhoGAPp190dsRNA.N.Scer\UAS
RhoGAPp190dsRNA.N.UAS
Name Synonyms
Secondary FlyBase IDs
    References (3)