FlyBase Allele Report: Dmel\rib[P7]

General Information

Symbol

Dmel\rib^P7

Species

D. melanogaster

Name

FlyBase ID

FBal0129105

Feature type

allele

Associated gene

Dmel\rib

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Shim et al., 2001)

Progenitor genotype

Cytology

Description

Amino acid replacement: W22term.

(Shim et al., 2001)

Nucleotide substitution: G?A.

(Shim et al., 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:19,278,194..19,278,194 [+]

Nucleotide change:

G19278194A

Reported nucleotide change:

TGG?TGA

Amino acid change:

W22term | rib-PA; W22term | rib-PB; W22term | rib-PC

Reported amino acid change:

W22term

(Shim et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity (with rib¹)

(Kerman et al., 2008)

decreased cell growth rate | embryonic stage (with rib¹)

(Loganathan et al., 2022)

decreased cell size | embryonic stage (with rib¹)

(Loganathan et al., 2022)

decreased size | embryonic stage (with rib¹)

(Loganathan et al., 2022)

lethal | recessive

(Shim et al., 2001)

Phenotype Manifest In

Detailed Description

Statement

Reference

The developing salivary glands in rib^P7 mutant animals fail to turn and migrate properly. In addition, the dorsal trunks of the trachea exhibit only partial extension at stage 14, but relatively normal extension of dorsal and visceral branches occurs. These defects are also observed in rib¹/rib^P7 animals.

rib¹/rib^P7 mutant animals have normal salivary gland epithelial cell architecture, but exhibit short lumena with distally tilted apical-basal cell axes. There are reduced numbers of apical vesicles and increased microvillar structure.

(Kerman et al., 2008)

Stage 13 rib^P7 embryos show little tracheal branch budding or outgrowth. By stage 14, 99% of tracheal branches remain stalled, although 1% have formed and grown out excessively and in abnormal directions.

(Shim et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

rib^P7/rib¹ has presumptive embryonic salivary gland phenotype, suppressible | partially by crb^intra.UAS/Scer\GAL4^btl.PS

(Kerman et al., 2008)

rib^P7/rib¹ has tracheal dorsal trunk primordium phenotype, suppressible | partially by Scer\GAL4^btl.PS/Moe^{T559A.UAS.Tag:MYC}

(Kerman et al., 2008)

NOT suppressed by

Statement

Reference

rib^P7/rib¹ has tracheal dorsal trunk primordium phenotype, non-suppressible by crb^intra.UAS/Scer\GAL4^btl.PS

(Kerman et al., 2008)

rib^P7/rib¹ has presumptive embryonic salivary gland phenotype, non-suppressible by Scer\GAL4^fkh.PH/crb^UAS.cWa

(Kerman et al., 2008)

rib^P7/rib¹ has tracheal dorsal trunk primordium phenotype, non-suppressible by crb^UAS.cWa/Scer\GAL4^btl.PS

(Kerman et al., 2008)

rib^P7/rib¹ has presumptive embryonic salivary gland phenotype, non-suppressible by Scer\GAL4^fkh.PH/Moe^{T559A.UAS.Tag:MYC}

(Kerman et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of crb^{intra.Scer\UAS} in the trachea under the control of Scer\GAL4^btl.PS in rib¹/rib^P7 mutant animals partially rescues the tracheal dorsal trunk extension defects.

Expression of crb^{intra.Scer\UAS} in the salivary gland under the control of Scer\GAL4^fkh.PH in rib¹/rib^P7 animals fails to rescue salivary gland migration defects.

Expression of crb^Scer\UAS.cWa in the salivary gland under the control of Scer\GAL4^fkh.PH in rib¹/rib^P7 animals fails to rescue salivary gland migration defects.

Expression of crb^Scer\UAS.cWa in the trachea under the control of Scer\GAL4^btl.PS in rib¹/rib^P7 animals fails to rescue tracheal defects.

Expression of Moe^{T559A.Scer\UAS.T:Hsap\MYC} in the trachea under the control of Scer\GAL4^btl.PS in rib¹/rib^P7 mutant animals partially rescues the dorsal trunk extension defects.

Expression of Moe^{T559A.Scer\UAS.T:Hsap\MYC} in the salivary gland under the control of Scer\GAL4^fkh.PH in rib¹/rib^P7 mutant animals fails to rescue the salivary gland migration defects.

(Kerman et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

rib²

(Shim et al., 2001)

rib¹

(Shim et al., 2001)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Based on the extent of salivary gland development, the following rib alleles can be ranked from strongest to weakest as follows: Df(2R)rib-ex12 > rib^P7 > rib¹/rib^P7 > rib¹.

(Kerman et al., 2008)

Based on the extent of tracheal development, the following rib alleles can be ranked from strongest to weakest as follows: Df(2R)rib-ex12 > rib¹/rib^P7 > rib¹ > rib^P7 .

(Kerman et al., 2008)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

rib^P7

(Loganathan et al., 2022, Loganathan et al., 2016, Kerman et al., 2008, Luschnig et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (5)

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