FB2025_01 , released February 20, 2025
Allele: Scer\GAL4JO15
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General Information
Symbol
Scer\GAL4JO15
Species
S. cerevisiae
Name
FlyBase ID
FBal0141511
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{J21.17-GAL4}
Also Known As
JO15, JO15-GAL4, JO-AB, JO15-2-GAL4, JO-AB-Gal4
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Sharma et al., 2002)
Progenitor genotype
Carried in construct
P{J21.17-GAL4}
Cytology
Description

A 5.8kb BamHI genomic fragment (which derives from the genome about 0.4kb from the H{Lw2} insertion site in the H{Lw2}J21.17 enhancer trap line) is fused upstream of a minimal promoter driving GAL4 expression.

(Sharma et al., 2002)
Allele components
Component
Use(s)
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      pupal stage
      zone A Johnston organ neuron
      (Lee et al., 2010)
      zone B Johnston organ neuron
      (Lee et al., 2010)
      pharate adult stage P9
      Johnston organ

      Comment: reference states 60 hr APF

      (Sarpal et al., 2003)
      Johnston organ neuron

      Comment: reference states 60 hr APF

      (Sarpal et al., 2003)
      sensory neuron | subset

      Comment: reference states 60 hr APF

      (Sarpal et al., 2003)
      adult stage
      zone B Johnston organ neuron | subset
      (Kamikouchi et al., 2006)
      zone A Johnston organ neuron | subset
      (Kamikouchi et al., 2006)
      zone B Johnston organ neuron
      (Kamikouchi et al., 2009, Yorozu et al., 2009)
      zone A Johnston organ neuron
      (Kamikouchi et al., 2009, Yorozu et al., 2009)
      sound activated Johnston organ neuron
      (Kamikouchi et al., 2009, Yorozu et al., 2009)
      adult brain | restricted
      (Hampel et al., 2015)
      adult ventral nerve cord | restricted
      (Hampel et al., 2015)
      subregion AA of AMMC zone A
      (Flybrain Neuron Database et al., 2018.6.28)
      subregion AD of AMMC zone A
      (Flybrain Neuron Database et al., 2018.6.28)
      subregion AP of AMMC zone A
      (Flybrain Neuron Database et al., 2018.6.28)
      subregion AV1 of AMMC zone A
      (Flybrain Neuron Database et al., 2018.6.28)
      subregion AV2 of AMMC zone A
      (Flybrain Neuron Database et al., 2018.6.28)
      subregion BO of AMMC zone B
      (Flybrain Neuron Database et al., 2018.6.28)
      Additional Information
      Statement
      Reference

      Drives expression in zone A and zone B Johnston organ neurons.

      (FlyBase Curators, 2017)

      Expression of ScerGAL4JO15 is seen in the ~50-100 neurons innervating AMMC zone A, and ~100-150 neurons innervating AMMC zone B.

      (Kamikouchi et al., 2009)
       
      Marker for
      zone A Johnston organ neuron
      (Lee et al., 2010)
      zone B Johnston organ neuron
      (Lee et al., 2010)
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Optogenetic activation (using Cnoc\ChR1Cs.IVS.20xUAS.Venus with red light activation) of Scer\GAL4JO15 neurons does not significantly affect the number of antennal grooming bouts compared to controls.

      (Hampel et al., 2015)

      Inhibition of neural activity in Scer\GAL4JO15-expressing neurons results in courtship defects.

      (Shankar et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Scer\GAL4JO15
      Name Synonyms
      Secondary FlyBase IDs
        References (22)