The increased cell proliferation in the adult gut expressing NdsRNA.P.Scer\UAS under the control of Scer\GAL4esg-NP5130 (using tub-gal80[ts] to limit the time of expression) is suppressed by co-expression of PEKGD5584.
A Tsc1Q87X mutant clone background results in the gradual loss of Tsc1Q87X NdsRNA.P.Scer\UAS double mutant intestinal stem cell clones from the epithelium, as only 11.3% intestinal stem cell clones are maintained by 21 days after clone induction, compared with a 94.8% maintenance rate for NdsRNA.P.Scer\UAS single mutants. There is no significant increase in apoptosis in these double mutant cells. At 2 weeks after clone induction, when significant numbers of Tsc1Q87X NdsRNA.P.Scer\UAS double mutant intestinal stem cells have been eliminated, many mutant cells have detached from the epithelium and are found in the lumen. Some delaminate as single cells while others delaminate in clusters.
Tsc1Q87X NdsRNA.P.Scer\UAS double mutant intestinal stem cell (ISC) clones are larger than wild-type clones. They are Dl-positive, indicating that they are ISC-like, remain diploid, and are negative for the epithelial cell marker nub. Enteroendocrine cell-like tumours are barely observed in these double mutants.
Apc2g10, ApcQ8 double mutant clones in the adult midgut that are also expressing NdsRNA.P.Scer\UAS under the control of Scer\GAL4tub often show extensive proliferation and multilayering 10 days after induction in the adult. The number of intestinal stem cells present and the mitotic index of the intestinal stem cells 5 days after induction is significantly higher in Apc2g10, ApcQ8 double mutant clones in the adult midgut that are also expressing NdsRNA.P.Scer\UAS under the control of Scer\GAL4tub compared to clones expressing NdsRNA.P.Scer\UAS under the control of Scer\GAL4tub in an otherwise wild-type background.
Expression of NdsRNA.P.Scer\UAS under the control of Scer\GAL4elav.PH in ewg2 motorneurons (these motorneurons have been obtained by using FLP/FRT mediated recombination to inactivate the ewgelav.PH rescuing transgene and simultaneously activate the Scer\GAL4elav.PH driver in a ewg2 background) results in intermediate numbers of boutons at the larval neuromuscular junction compared to wild-type and ewg2 motorneurons.