Adulthood-only expression of NdsRNA.P.UAS under the control of Scer\GAL4NP5130 (and GAL80[ts] for the temporal control of expression) leads to the the development of midgut ISC-like tumors.
NdsRNA.P.UAS expression only during adulthood under the combined control of Scer\GAL4GMR35H09 and Gal80[ts] leads to significantly fewer nuclei per indirect flight muscle in 10-days old adults, as compared to controls.
Third instar larval eye-antennal discs expressing NdsRNA.P.Scer\UAS under the control of Scer\GAL4hth-GETDB show a disruption of epithelial folding at the A1 fold, as compared to controls.
Expression of NdsRNA.P.Scer\UAS under the control of Scer\GAL4en.PU results in a significant decrease in size of the third instar wing disc posterior compartment, as compared to controls.
Expression of NdsRNA.P.Scer\UAS under the control of Scer\GAL4esg-NP5130 (using tub-gal80[ts] to limit the time of expression) increases cell proliferation in the adult gut.
The temporally regulated expression (by Gal80[ts]) of NdsRNA.P.UAS, for 6 days under the control of Scer\GAL4NP5130, leads to a strong increase in the combined population of intestinal stem cells and enteroendocrine cells, assessed by immuno-staining for Delta and Prospero, respectively, in the adult midgut, as compared to controls.
Expression of NdsRNA.P.Scer\UAS under the control of Scer\GAL4ato.3.6 results in a significant increase in the number of medulla axons compared to wild type.
Expression of NdsRNA.P.Scer\UAS under the control of Scer\GAL4ato.3.6 in the dorsal cluster neurons specifically during the pupal stage (using the temperature-sensitive Scer\GAL80ts.αTub84B allele to limit the timing of expression) results in an increase in the number of medulla axons when expression occurs between 24 and 60 hours after puparium formation.
NdsRNA.P.Scer\UAS intestinal stem cell (ISC) clones develop into both Dl-positive ISC-like and pros-positive ee cell-like tumours with a 1:1 ratio.
Embryos co-expressing NKK102890 and NdsRNA.P.Scer\UAS under the control of Scer\GAL4elav-C155 (in the presence of Dcr-2Scer\UAS.cDa to enhance RNAi) have an increased number of "Ap" neurons in the NB5-6T lineage.
Expression of NdsRNA.P.Scer\UAS under the control of Scer\GAL4pnt-14-94 results in the progressive loss of type II neuroblasts in larvae.
Late third instar wing discs expressing NdsRNA.P.Scer\UAS under the control of Scer\GAL4vg.int2.1 have greatly disturbed dorsal-ventral (DV) boundaries compared to controls. BrdU staining shows that increased boundary cell proliferation is occurring at the DV boundary.
Males expressing NdsRNA.P.Scer\UAS under the control of Scer\GAL4Abd-B-LDN show normal genitalia rotation.
When NdsRNA.P.Scer\UAS is expressed under the control of Scer\GAL4bbg-C96, the wing margin is dramatically scalloped and almost devoid of trichomes.
When NdsRNA.P.Scer\UAS is expressed under the control of Scer\GAL4pnr-MD237, a disruption of the pattern of sensory bristles on the thorax and balding is apparent.
Expression of NdsRNA.P.Scer\UAS under the control of Scer\GAL4GMR.PF results in rough eyes.
Expression of NdsRNA.P.Scer\UAS under the control of Scer\GAL4elav.PH in clones induced by FLP/FRT mediated recombination (to activate the Scer\GAL4elav.PH driver) results in an increase in the number of type 1b boutons at muscle 13 compared to wild type.
Expression of NdsRNA.P.Scer\UAS under the control of Scer\GAL4en-e16E results in a range of phenotypes in the wing, depending on the insertion line used. The phenotypes range from no mutant phenotype (P{UAS-N.RNAi.M}attP3), to a predominantly vein thickening phenotype (P{UAS-N.RNAi.M}attP2), to a severe vein thickening defect often accompanied by wing notches (P{UAS-N.RNAi.M}attP40).
Adults carrying NdsRNA.P.Scer\UAS, Scer\GAL4esg-NP5130 and Scer\GAL80ts.αTub84B and raised at the permissive temperature have wild-type midguts (at this temperature, the Scer\GAL80ts.αTub84B protein inhibits Scer\GAL4esg-NP5130). When these flies are shifted to the non-permissive temperature (which inactivates Scer\GAL80ts.αTub84B, allowing expression of NdsRNA.P.Scer\UAS under the control of Scer\GAL4esg-NP5130), there is an increase in the number of small cells in the adult midgut epithelium.
When NdsRNA.P.Scer\UAS is driven by Scer\GAL4c747, short term memory is normal, however long term memory is impaired.
Expression of NdsRNA.P.Scer\UAS under the control of Scer\GAL4C96 results in a number of wing phenotypes depending on the P{UAS-N.dsRNA.P} insertion line used, ranging from no defects in wing margin formation, through subtle bristle defects, a consistent notched phenotype to extreme loss of dorsal and ventral wing tissue. Expression of NdsRNA.P.Scer\UAS under the control of Scer\GAL4hs.2sev or Scer\GAL4GMR.PF results in a rough eye phenotype. Animals expressing NdsRNA.P.Scer\UAS under the control of Scer\GAL4pnr-MD237 show balding of the thorax and either loss of notal macrochaetae or multiplication of macrochaetae (depending on the P{UAS-N.dsRNA.P} insertion line used). Animals expressing NdsRNA.P.Scer\UAS under the control of Scer\GAL4A307 have a markedly impaired startle response to proprionic acid vapour compared to controls.
NdsRNA.P.UAS, Scer\GAL4NP5130 has increased cell number | adult stage phenotype, enhanceable by Xbp1GD4745, Scer\GAL4NP5130
NdsRNA.P.UAS, Scer\GAL4NP5130 has neoplasia | adult stage phenotype, suppressible by Sox100BNIG.15552R, Scer\GAL4NP5130
NdsRNA.P.UAS, Scer\GAL4NP5130 has neoplasia | adult stage phenotype, suppressible by Sox100BUAS.cJa, Scer\GAL4NP5130
NdsRNA.P.UAS, Scer\GAL4NP5130 has increased occurrence of cell division phenotype, suppressible by PEKGD5584, Scer\GAL4NP5130
NdsRNA.P.UAS, Scer\GAL4NP5130 has increased cell number | adult stage phenotype, suppressible by Xbp1UAS.RB, Scer\GAL4NP5130
Scer\GAL4FRT.Act5C/NdsRNA.P.UAS is a suppressor of increased cell number phenotype of elB3.3.1, nocd64
Scer\GAL4FRT.Act5C/NdsRNA.P.UAS is a suppressor of neoplasia | adult stage phenotype of elB3.3.1, nocd64
NdsRNA.P.UAS, Scer\GAL4NP5130 has intestinal stem cell | adult stage phenotype, enhanceable by Xbp1GD4745, Scer\GAL4NP5130
NdsRNA.P.UAS, Scer\GAL4NP5130 has enteroendocrine cell | adult stage phenotype, enhanceable by Xbp1GD4745, Scer\GAL4NP5130
NdsRNA.P.UAS, Scer\GAL4NP5130 has midgut | adult stage phenotype, enhanceable by Xbp1GD4745, Scer\GAL4NP5130
NdsRNA.P.UAS, Scer\GAL4NP5130 has intestinal stem cell | increased number | adult stage phenotype, suppressible by Sox100BNIG.15552R, Scer\GAL4NP5130
NdsRNA.P.UAS, Scer\GAL4NP5130 has enteroblast | increased number | adult stage phenotype, suppressible by Sox100BNIG.15552R, Scer\GAL4NP5130
NdsRNA.P.UAS, Scer\GAL4NP5130 has adult midgut epithelium | adult stage phenotype, suppressible by Sox100BNIG.15552R, Scer\GAL4NP5130
NdsRNA.P.UAS, Scer\GAL4NP5130 has intestinal stem cell | increased number | adult stage phenotype, suppressible by Sox100BUAS.cJa, Scer\GAL4NP5130
NdsRNA.P.UAS, Scer\GAL4NP5130 has enteroblast | increased number | adult stage phenotype, suppressible by Sox100BUAS.cJa, Scer\GAL4NP5130
NdsRNA.P.UAS, Scer\GAL4NP5130 has adult midgut epithelium | adult stage phenotype, suppressible by Sox100BUAS.cJa, Scer\GAL4NP5130
NdsRNA.P.UAS, Scer\GAL4NP5130 has adult gut phenotype, suppressible by PEKGD5584, Scer\GAL4NP5130
NdsRNA.P.UAS, Scer\GAL4NP5130 has intestinal stem cell | adult stage phenotype, suppressible by Xbp1UAS.RB, Scer\GAL4NP5130
NdsRNA.P.UAS, Scer\GAL4NP5130 has enteroendocrine cell | adult stage phenotype, suppressible by Xbp1UAS.RB, Scer\GAL4NP5130
NdsRNA.P.UAS, Scer\GAL4NP5130 has midgut | adult stage phenotype, suppressible by Xbp1UAS.RB, Scer\GAL4NP5130
NdsRNA.P.UAS has dorsal-ventral compartment boundary of the wing disc | third instar larval stage phenotype, suppressible | partially by +/Df(3L)mir-banΔ1
NdsRNA.P.UAS has dorsal-ventral compartment boundary of the wing disc | third instar larval stage phenotype, suppressible | partially by Dcr-1GD11429/Scer\GAL4vg.int2.1
Scer\GAL4Abd-B-LDN/NdsRNA.P.UAS is a suppressor | partially of male genitalia phenotype of UvragKG04163/UvragB21
Scer\GAL4FRT.Act5C/NdsRNA.P.UAS is a suppressor of eye-antennal disc | somatic clone phenotype of elB3.3.1, nocd64
Scer\GAL4FRT.Act5C/NdsRNA.P.UAS is a suppressor of head capsule | somatic clone phenotype of elB3.3.1, nocd64
NdsRNA.P.UAS, Scer\GAL4NP5130, Tsc1Q87X has intestinal stem cell | somatic clone phenotype
Apc2g10, ApcQ8, NdsRNA.P.UAS, Scer\GAL4Tub.PU has intestinal stem cell | somatic clone phenotype
The increased cell proliferation in the adult gut expressing NdsRNA.P.Scer\UAS under the control of Scer\GAL4esg-NP5130 (using tub-gal80[ts] to limit the time of expression) is suppressed by co-expression of PEKGD5584.
A Tsc1Q87X mutant clone background results in the gradual loss of Tsc1Q87X NdsRNA.P.Scer\UAS double mutant intestinal stem cell clones from the epithelium, as only 11.3% intestinal stem cell clones are maintained by 21 days after clone induction, compared with a 94.8% maintenance rate for NdsRNA.P.Scer\UAS single mutants. There is no significant increase in apoptosis in these double mutant cells. At 2 weeks after clone induction, when significant numbers of Tsc1Q87X NdsRNA.P.Scer\UAS double mutant intestinal stem cells have been eliminated, many mutant cells have detached from the epithelium and are found in the lumen. Some delaminate as single cells while others delaminate in clusters.
Tsc1Q87X NdsRNA.P.Scer\UAS double mutant intestinal stem cell (ISC) clones are larger than wild-type clones. They are Dl-positive, indicating that they are ISC-like, remain diploid, and are negative for the epithelial cell marker nub. Enteroendocrine cell-like tumours are barely observed in these double mutants.
One copy of Df(3L)banΔ1 largely rescues the dorsal-ventral boundary defects seen when NdsRNA.P.Scer\UAS is expressed under the control of Scer\GAL4vg.int2.1.
Expression of Dcr-1GD11429 largely rescues the dorsal-ventral boundary defects seen when NdsRNA.P.Scer\UAS is expressed under the control of Scer\GAL4vg.int2.1.
Expression of bansponge.Scer\UAS.T:Disc\RFP largely rescues the dorsal-ventral boundary defects seen when NdsRNA.P.Scer\UAS is expressed under the control of Scer\GAL4vg.int2.1.
Expression of P{UAS-N.dsRNA.P} using Scer\GAL4Abd-B-LDN partially rescues the genital rotation defect in UvragKG04163/UvragB21 males.
Apc2g10, ApcQ8 double mutant clones in the adult midgut that are also expressing NdsRNA.P.Scer\UAS under the control of Scer\GAL4tub often show extensive proliferation and multilayering 10 days after induction in the adult. The number of intestinal stem cells present and the mitotic index of the intestinal stem cells 5 days after induction is significantly higher in Apc2g10, ApcQ8 double mutant clones in the adult midgut that are also expressing NdsRNA.P.Scer\UAS under the control of Scer\GAL4tub compared to clones expressing NdsRNA.P.Scer\UAS under the control of Scer\GAL4tub in an otherwise wild-type background.
Expression of NdsRNA.P.Scer\UAS under the control of Scer\GAL4elav.PH in ewg2 motorneurons (these motorneurons have been obtained by using FLP/FRT mediated recombination to inactivate the ewgelav.PH rescuing transgene and simultaneously activate the Scer\GAL4elav.PH driver in a ewg2 background) results in intermediate numbers of boutons at the larval neuromuscular junction compared to wild-type and ewg2 motorneurons.
A reduction of N protein levels in elB3.3.1 nocd64 double mutant ey clones, through expression of NdsRNA.P.Scer\UAS under the control of Scer\GAL4Scer\FRT.Act5C reduces the frequency of overgrowths per adult fly from 51.5% to 2.2%, without affecting cell viability.
The P{UAS-N.RNAi.M} transgene has been introduced into 3 different phiC31\attP docking sites in the genome, to produce an allelic series.