FlyBase Allele Report: Dmel\N[dsRNA.P.UAS]

General Information

Symbol

Dmel\N^dsRNA.P.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0141643

Feature type

allele

Associated gene

Dmel\N

Associated Insertion(s)

Carried in Construct

P{UAS-N.dsRNA.P}

P{UAS-N.RNAi.M}

Also Known As

UAS-Notch-RNAi, UAS-N^RNAi, N RNAi, UAS-N^dsRNA, UAS-Ni, UAS-N-dsRNA, UAS-NotchRNAi, N-IR

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

in vitro construct - regulatory fusion

(Markstein et al., 2008, Presente et al., 2002)

in vitro construct - RNAi

(Presente et al., 2002)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{UAS-N.dsRNA.P}

P{UAS-N.RNAi.M}

Cytology

Nature of the lesion

Statement

Reference

UASt regulatory sequences drive expression of N sequences arranged in an inverted repeat (each copy of the repeat is a 1001bp fragment from N exon 6).

(Presente et al., 2002)

Allele components

Product class / Tool use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates carcinoma

modeled by Vps25^A3

(Vaccari and Bilder, 2005)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

abnormal chemosensitive behavior

(Presente et al., 2002)

abnormal memory, with Scer\GAL4^c747

(Presente et al., 2004)

abnormal neuroanatomy | adult stage, with Scer\GAL4^ato.3.6

(Langen et al., 2013)

abnormal neuroanatomy | embryonic stage, with Dcr-2^UAS.cDa, N^KK102890, Scer\GAL4^elav-C155

(Ulvklo et al., 2012)

abnormal neuroanatomy | larval stage, with Scer\GAL4^pnt-14-94

(Zhu et al., 2012)

abnormal neuroanatomy | somatic clone | third instar larval stage, with Scer\GAL4^elav.PH

(Haussmann et al., 2008)

abnormal size | third instar larval stage, with Scer\GAL4^en.PU

(Slaninova et al., 2016)

increased occurrence of cell division, with Scer\GAL4^NP5130

(Wang et al., 2015)

increased occurrence of cell division | third instar larval stage, with Scer\GAL4^vg.int2.1

(Becam et al., 2011)

visible, with Scer\GAL4^bbg-C96

(Boyles et al., 2010, Presente et al., 2002)

visible, with Scer\GAL4^en-e16E

(Markstein et al., 2008)

visible, with Scer\GAL4^GMR.PF

(Boyles et al., 2010, Presente et al., 2002)

visible, with Scer\GAL4^hs.2sev

(Presente et al., 2002)

visible, with Scer\GAL4^pnr-MD237

(Boyles et al., 2010, Presente et al., 2002)

Phenotype Manifest In

adult external thorax, with Scer\GAL4^pnr-MD237

(Boyles et al., 2010)

adult gut, with Scer\GAL4^NP5130

(Wang et al., 2015)

adult midgut | heat sensitive, with Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B

(Micchelli and Perrimon, 2006)

adult thoracic sensillum, with Scer\GAL4^pnr-MD237

(Boyles et al., 2010)

adult thorax, with Scer\GAL4^pnr-MD237

(Presente et al., 2002)

apterous-expressing neuron of the lateral cluster of the thorax, with Dcr-2^UAS.cDa, N^KK102890, Scer\GAL4^elav-C155

(Ulvklo et al., 2012)

dorsal-ventral compartment boundary of the wing disc | third instar larval stage, with Scer\GAL4^vg.int2.1

(Becam et al., 2011)

embryonic/larval neuromuscular junction | somatic clone, with Scer\GAL4^elav.PH

(Haussmann et al., 2008)

eye, with Scer\GAL4^GMR.PF

(Boyles et al., 2010, Presente et al., 2002)

eye, with Scer\GAL4^hs.2sev

(Presente et al., 2002)

eye-antennal disc | third instar larval stage, with Scer\GAL4^hth-GETDB

(Ku and Sun, 2017)

macrochaeta, with Scer\GAL4^pnr-MD237

(Presente et al., 2002)

macrochaeta | increased number, with Scer\GAL4^pnr-MD237

(Presente et al., 2002)

medulla, with Scer\GAL4^ato.3.6

(Langen et al., 2013)

NMJ bouton | somatic clone, with Scer\GAL4^elav.PH

(Haussmann et al., 2008)

nucleus | adult stage, with Scer\GAL4^GMR35H09

(Boukhatmi and Bray, 2018)

ommatidium, with Scer\GAL4^GMR.PF

(Presente et al., 2002)

ommatidium, with Scer\GAL4^hs.2sev

(Presente et al., 2002)

type II neuroblast | larval stage, with Scer\GAL4^pnt-14-94

(Zhu et al., 2012)

wing, with Scer\GAL4^bbg-C96

(Presente et al., 2002)

wing, with Scer\GAL4^en-e16E

(Markstein et al., 2008)

wing disc posterior compartment | third instar larval stage, with Scer\GAL4^en.PU

(Slaninova et al., 2016)

wing margin, with Scer\GAL4^bbg-C96

(Boyles et al., 2010, Presente et al., 2002)

wing vein, with Scer\GAL4^en-e16E

(Markstein et al., 2008)

Detailed Description

Statement

Reference

N^dsRNA.P.UAS expression only during adulthood under the combined control of Scer\GAL4^GMR35H09 and Gal80[ts] leads to significantly fewer nuclei per indirect flight muscle in 10-days old adults, as compared to controls.

(Boukhatmi and Bray, 2018)

Third instar larval eye-antennal discs expressing N^{dsRNA.P.Scer\UAS} under the control of Scer\GAL4^hth-GETDB show a disruption of epithelial folding at the A1 fold, as compared to controls.

(Ku and Sun, 2017)

Expression of N^{dsRNA.P.Scer\UAS} under the control of Scer\GAL4^en.PU results in a significant decrease in size of the third instar wing disc posterior compartment, as compared to controls.

(Slaninova et al., 2016)

Expression of N^{dsRNA.P.Scer\UAS} under the control of Scer\GAL4^esg-NP5130 (using tub-gal80[ts] to limit the time of expression) increases cell proliferation in the adult gut.

(Wang et al., 2015)

Expression of N^{dsRNA.P.Scer\UAS} under the control of Scer\GAL4^ato.3.6 results in a significant increase in the number of medulla axons compared to wild type.

Expression of N^{dsRNA.P.Scer\UAS} under the control of Scer\GAL4^ato.3.6 in the dorsal cluster neurons specifically during the pupal stage (using the temperature-sensitive Scer\GAL80^ts.αTub84B allele to limit the timing of expression) results in an increase in the number of medulla axons when expression occurs between 24 and 60 hours after puparium formation.

(Langen et al., 2013)

N^{dsRNA.P.Scer\UAS} intestinal stem cell (ISC) clones develop into both Dl-positive ISC-like and pros-positive ee cell-like tumours with a 1:1 ratio.

(Quan et al., 2013)

Embryos co-expressing N^KK102890 and N^{dsRNA.P.Scer\UAS} under the control of Scer\GAL4^elav-C155 (in the presence of Dcr-2^Scer\UAS.cDa to enhance RNAi) have an increased number of "Ap" neurons in the NB5-6T lineage.

(Ulvklo et al., 2012)

Expression of N^{dsRNA.P.Scer\UAS} under the control of Scer\GAL4^pnt-14-94 results in the progressive loss of type II neuroblasts in larvae.

(Zhu et al., 2012)

Late third instar wing discs expressing N^{dsRNA.P.Scer\UAS} under the control of Scer\GAL4^vg.int2.1 have greatly disturbed dorsal-ventral (DV) boundaries compared to controls. BrdU staining shows that increased boundary cell proliferation is occurring at the DV boundary.

(Becam et al., 2011)

Males expressing N^{dsRNA.P.Scer\UAS} under the control of Scer\GAL4^Abd-B-LDN show normal genitalia rotation.

(Lee et al., 2011)

When N^{dsRNA.P.Scer\UAS} is expressed under the control of Scer\GAL4^bbg-C96, the wing margin is dramatically scalloped and almost devoid of trichomes.

When N^{dsRNA.P.Scer\UAS} is expressed under the control of Scer\GAL4^pnr-MD237, a disruption of the pattern of sensory bristles on the thorax and balding is apparent.

Expression of N^{dsRNA.P.Scer\UAS} under the control of Scer\GAL4^GMR.PF results in rough eyes.

(Boyles et al., 2010)

Expression of N^{dsRNA.P.Scer\UAS} under the control of Scer\GAL4^elav.PH in clones induced by FLP/FRT mediated recombination (to activate the Scer\GAL4^elav.PH driver) results in an increase in the number of type 1b boutons at muscle 13 compared to wild type.

(Haussmann et al., 2008)

Expression of N^{dsRNA.P.Scer\UAS} under the control of Scer\GAL4^en-e16E results in a range of phenotypes in the wing, depending on the insertion line used. The phenotypes range from no mutant phenotype (P{UAS-N.RNAi.M}attP3), to a predominantly vein thickening phenotype (P{UAS-N.RNAi.M}attP2), to a severe vein thickening defect often accompanied by wing notches (P{UAS-N.RNAi.M}attP40).

(Markstein et al., 2008)

Adults carrying N^{dsRNA.P.Scer\UAS}, Scer\GAL4^esg-NP5130 and Scer\GAL80^ts.αTub84B and raised at the permissive temperature have wild-type midguts (at this temperature, the Scer\GAL80^ts.αTub84B protein inhibits Scer\GAL4^esg-NP5130). When these flies are shifted to the non-permissive temperature (which inactivates Scer\GAL80^ts.αTub84B, allowing expression of N^{dsRNA.P.Scer\UAS} under the control of Scer\GAL4^esg-NP5130), there is an increase in the number of small cells in the adult midgut epithelium.

(Micchelli and Perrimon, 2006)

When N^{dsRNA.P.Scer\UAS} is driven by Scer\GAL4^c747, short term memory is normal, however long term memory is impaired.

(Presente et al., 2004)

Expression of N^{dsRNA.P.Scer\UAS} under the control of Scer\GAL4^C96 results in a number of wing phenotypes depending on the P{UAS-N.dsRNA.P} insertion line used, ranging from no defects in wing margin formation, through subtle bristle defects, a consistent notched phenotype to extreme loss of dorsal and ventral wing tissue. Expression of N^{dsRNA.P.Scer\UAS} under the control of Scer\GAL4^hs.2sev or Scer\GAL4^GMR.PF results in a rough eye phenotype. Animals expressing N^{dsRNA.P.Scer\UAS} under the control of Scer\GAL4^pnr-MD237 show balding of the thorax and either loss of notal macrochaetae or multiplication of macrochaetae (depending on the P{UAS-N.dsRNA.P} insertion line used). Animals expressing N^{dsRNA.P.Scer\UAS} under the control of Scer\GAL4^A307 have a markedly impaired startle response to proprionic acid vapour compared to controls.

(Presente et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

N^dsRNA.P.UAS, Scer\GAL4^NP5130 has increased occurrence of cell division phenotype, suppressible by PEK^GD5584, Scer\GAL4^NP5130

(Wang et al., 2015)

Suppressor of

Statement

Reference

Scer\GAL4^FRT.Act5C/N^dsRNA.P.UAS is a suppressor of increased cell number phenotype of elB^3.3.1, noc^d64

(Luque and Milan, 2007)

Scer\GAL4^FRT.Act5C/N^dsRNA.P.UAS is a suppressor of neoplasia | adult stage phenotype of elB^3.3.1, noc^d64

(Luque and Milan, 2007)

Other

Statement

Reference

Apc2^g10, Apc^Q8, N^dsRNA.P.UAS, Scer\GAL4^Tub.PU has increased occurrence of cell division | somatic clone phenotype

(Lee et al., 2009)

Phenotype Manifest In

Suppressed by

Statement

Reference

N^dsRNA.P.UAS, Scer\GAL4^NP5130 has adult gut phenotype, suppressible by PEK^GD5584, Scer\GAL4^NP5130

(Wang et al., 2015)

N^dsRNA.P.UAS has dorsal-ventral compartment boundary of the wing disc | third instar larval stage phenotype, suppressible | partially by +/Df(3L)mir-ban^Δ1

(Becam et al., 2011)

N^dsRNA.P.UAS has dorsal-ventral compartment boundary of the wing disc | third instar larval stage phenotype, suppressible | partially by Dcr-1^GD11429/Scer\GAL4^vg.int2.1

(Becam et al., 2011)

N^dsRNA.P.UAS, Scer\GAL4^vg.int2.1 has dorsal-ventral compartment boundary of the wing disc | third instar larval stage phenotype, suppressible | partially by Disc\RFP^{dsRed.Scer\UAS.ban-sponge}, Scer\GAL4^vg.int2.1

(Becam et al., 2011)

Suppressor of

Statement

Reference

Scer\GAL4^Abd-B-LDN/N^dsRNA.P.UAS is a suppressor | partially of male genitalia phenotype of Uvrag^KG04163/Uvrag^B21

(Lee et al., 2011)

Scer\GAL4^FRT.Act5C/N^dsRNA.P.UAS is a suppressor of eye-antennal disc | somatic clone phenotype of elB^3.3.1, noc^d64

(Luque and Milan, 2007)

Scer\GAL4^FRT.Act5C/N^dsRNA.P.UAS is a suppressor of head capsule | somatic clone phenotype of elB^3.3.1, noc^d64

(Luque and Milan, 2007)

Other

Statement

Reference

N^dsRNA.P.UAS, Scer\GAL4^NP5130, Tsc1^Q87X has intestinal stem cell | somatic clone phenotype

(Quan et al., 2013)

Apc2^g10, Apc^Q8, N^dsRNA.P.UAS, Scer\GAL4^Tub.PU has intestinal stem cell | somatic clone phenotype

(Lee et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The increased cell proliferation in the adult gut expressing N^{dsRNA.P.Scer\UAS} under the control of Scer\GAL4^esg-NP5130 (using tub-gal80[ts] to limit the time of expression) is suppressed by co-expression of PEK^GD5584.

(Wang et al., 2015)

A Tsc1^Q87X mutant clone background results in the gradual loss of Tsc1^Q87X N^{dsRNA.P.Scer\UAS} double mutant intestinal stem cell clones from the epithelium, as only 11.3% intestinal stem cell clones are maintained by 21 days after clone induction, compared with a 94.8% maintenance rate for N^{dsRNA.P.Scer\UAS} single mutants. There is no significant increase in apoptosis in these double mutant cells. At 2 weeks after clone induction, when significant numbers of Tsc1^Q87X N^{dsRNA.P.Scer\UAS} double mutant intestinal stem cells have been eliminated, many mutant cells have detached from the epithelium and are found in the lumen. Some delaminate as single cells while others delaminate in clusters.

Tsc1^Q87X N^{dsRNA.P.Scer\UAS} double mutant intestinal stem cell (ISC) clones are larger than wild-type clones. They are Dl-positive, indicating that they are ISC-like, remain diploid, and are negative for the epithelial cell marker nub. Enteroendocrine cell-like tumours are barely observed in these double mutants.

(Quan et al., 2013)

One copy of Df(3L)ban^Δ1 largely rescues the dorsal-ventral boundary defects seen when N^{dsRNA.P.Scer\UAS} is expressed under the control of Scer\GAL4^vg.int2.1.

Expression of Dcr-1^GD11429 largely rescues the dorsal-ventral boundary defects seen when N^{dsRNA.P.Scer\UAS} is expressed under the control of Scer\GAL4^vg.int2.1.

Expression of ban^{sponge.Scer\UAS.T:Disc\RFP} largely rescues the dorsal-ventral boundary defects seen when N^{dsRNA.P.Scer\UAS} is expressed under the control of Scer\GAL4^vg.int2.1.

(Becam et al., 2011)

Expression of P{UAS-N.dsRNA.P} using Scer\GAL4^Abd-B-LDN partially rescues the genital rotation defect in Uvrag^KG04163/Uvrag^B21 males.

(Lee et al., 2011)

Apc2^g10, Apc^Q8 double mutant clones in the adult midgut that are also expressing N^{dsRNA.P.Scer\UAS} under the control of Scer\GAL4^tub often show extensive proliferation and multilayering 10 days after induction in the adult. The number of intestinal stem cells present and the mitotic index of the intestinal stem cells 5 days after induction is significantly higher in Apc2^g10, Apc^Q8 double mutant clones in the adult midgut that are also expressing N^{dsRNA.P.Scer\UAS} under the control of Scer\GAL4^tub compared to clones expressing N^{dsRNA.P.Scer\UAS} under the control of Scer\GAL4^tub in an otherwise wild-type background.

(Lee et al., 2009)

Expression of N^{dsRNA.P.Scer\UAS} under the control of Scer\GAL4^elav.PH in ewg² motorneurons (these motorneurons have been obtained by using FLP/FRT mediated recombination to inactivate the ewg^elav.PH rescuing transgene and simultaneously activate the Scer\GAL4^elav.PH driver in a ewg² background) results in intermediate numbers of boutons at the larval neuromuscular junction compared to wild-type and ewg² motorneurons.

(Haussmann et al., 2008)

A reduction of N protein levels in elB^3.3.1 noc^d64 double mutant ey clones, through expression of N^{dsRNA.P.Scer\UAS} under the control of Scer\GAL4^{Scer\FRT.Act5C} reduces the frequency of overgrowths per adult fly from 51.5% to 2.2%, without affecting cell viability.

(Luque and Milan, 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (4)

Bloomington

7077

w^*; P{UAS-N.dsRNA.P}9G

7078

P{UAS-N.dsRNA.P}14E, w^*

86307

P{hsFLP}12, P{tubP-GAL80}LL1 w^* P{neoFRT}19A; P{UAS-N.dsRNA.P}2; P{tubP-GAL4}LL7 P{neoFRT}82B P{tubP-GAL80}LL3/TM6C, Sb¹ Tb¹

86312

P{hsFLP}12, P{UAS-GFP.U}1, y¹ w^*; P{UAS-N.dsRNA.P}2/CyO; P{tubP-GAL4}LL7 P{neoFRT}82B P{tubP-GAL80}LL3/TM6C, Sb¹ Tb¹

Notes on Origin

Discoverer

Comments

The P{UAS-N.RNAi.M} transgene has been introduced into 3 different phiC31\attP docking sites in the genome, to produce an allelic series.

(Markstein et al., 2008)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

N^{dsRNA.P.Scer\UAS}

N^dsRNA.P.UAS

Notch^RNAi

(Wang et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (43)

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