FlyBase Allele Report: Dmel\EcR[B1-ΔC655.W650A.UAS]

UASt regulatory sequences drive expression of a mutated EcR B1 isoform which ends at EcR amino acid residue 655 and has the amino acid replacement W650A.

(Cherbas et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

EcR

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell number | larval stage, with Scer\GAL4^GMR31H09

(Lee et al., 2024)

abnormal cell shape | somatic clone, with Scer\GAL4^e22c

(Boyle and Berg, 2009)

abnormal developmental rate, with Scer\GAL4^Lsp2.PH

(Liu et al., 2013)

abnormal neuroanatomy

(Brown and Truman, 2009)

abnormal neuroanatomy, with Scer\GAL4^109(2)80

(Kirilly et al., 2009)

abnormal neuroanatomy, with Scer\GAL4^alrm.PD

(Tasdemir-Yilmaz and Freeman, 2014)

abnormal neuroanatomy, with Scer\GAL4^FMRFa.PS

(Brown et al., 2006)

abnormal neuroanatomy, with Scer\GAL4^ppk.1.9

(Ou et al., 2008)

abnormal neuroanatomy, with Scer\GAL4^αTub84B.PP

(Roy et al., 2007)

abnormal neuroanatomy | early third instar larval stage, with Scer\GAL4^CCAP.PP

(Lee et al., 2019)

abnormal neuroanatomy | larval stage, with Scer\GAL4^GMR31H09

(Lee et al., 2024)

abnormal neuroanatomy | P-stage, with Scer\GAL4^ppk.PG

(Sung et al., 2024)

abnormal neuroanatomy | P-stage, with Scer\GAL4^ppk.PU

(Zhang et al., 2014)

abnormal neuroanatomy | pupal stage, with Scer\GAL4^Crz.PU

(Lee et al., 2019)

abnormal neuroanatomy | second instar larval stage, with Scer\GAL4^CCAP.PP

(Lee et al., 2019)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^Burs.PL

(Lee et al., 2019)

abnormal neuroanatomy | wandering third instar larval stage, with Scer\GAL4^CCAP.PP

(Lee et al., 2019)

abnormal size | pupal stage, with Scer\GAL4^CCAP.PP

(Lee et al., 2019)

decreased cell death, with Scer\GAL4^Crz.PC

(Choi et al., 2006)

decreased cell death | pupal stage, with Scer\GAL4^Crz.PU

(Lee et al., 2019)

decreased cell size | somatic clone, with Scer\GAL4^e22c

(Boyle and Berg, 2009)

decreased size | larval stage, with Scer\GAL4^GMR31H09

(Lee et al., 2024)

increased cell death | early third instar larval stage, with Scer\GAL4^CCAP.PP

(Lee et al., 2019)

increased cell death | second instar larval stage, with Scer\GAL4^CCAP.PP

(Lee et al., 2019)

increased cell death | third instar larval stage, with Scer\GAL4^Burs.PL

(Lee et al., 2019)

increased cell death | wandering third instar larval stage, with Scer\GAL4^CCAP.PP

(Lee et al., 2019)

lethal, with Scer\GAL4^A9

(Lee et al., 2019)

lethal - all die before end of pupal stage, with Scer\GAL4^Tab2-201Y

(Hoopfer et al., 2008)

lethal - all die during embryonic stage, with Scer\GAL4^Tub.PU

(Lee et al., 2019)

lethal - all die during larval stage, with Scer\GAL4^r4

(Lee et al., 2019)

long lived | RU486 conditional, with Scer\GAL4^da.Switch.PT

(Tricoire et al., 2009)

melanotic mass phenotype | larval stage, with Scer\GAL4^A9

(Lee et al., 2019)

short lived | female | RU486 conditional, with Scer\GAL4^da.Switch.PT

(Tricoire et al., 2009)

short lived | RU486 conditional, with Scer\GAL4^da.Switch.PT

(Tricoire et al., 2009)

some die during larval stage, with Scer\GAL4^A9

(Lee et al., 2019)

some die during pupal stage, with Scer\GAL4^A9

(Lee et al., 2019)

some die during pupal stage, with Scer\GAL4^Tab2-201Y

(Hoopfer et al., 2008)

viable, with Scer\GAL4^ey-OK107

(Hoopfer et al., 2008)

visible | pupal stage, with Scer\GAL4^CCAP.PP

(Lee et al., 2019)

Phenotype Manifest In

adult CSD interneuron, with Scer\GAL4^αTub84B.PP

(Roy et al., 2007)

amnioserosa, with Scer\GAL4^da.G32

(Mohseni et al., 2009)

astrocyte-like glial cell, with Scer\GAL4^alrm.PD

(Tasdemir-Yilmaz and Freeman, 2014)

astrocyte-like glial cell | somatic clone, with Scer\GAL4^alrm.PD

(Tasdemir-Yilmaz and Freeman, 2014)

autolysosome, with Scer\GAL4^Lsp2.PH

(Liu et al., 2013)

autophagosome, with Scer\GAL4^Lsp2.PH

(Rusten et al., 2004)

cephalopharyngeal skeleton, with Scer\GAL4^69B

(Chavoshi et al., 2010)

dendrite, with Scer\GAL4^109(2)80

(Kirilly et al., 2009)

dendrite, with Scer\GAL4^ppk.1.9

(Ou et al., 2008)

dendrite | P-stage, with Scer\GAL4^ppk.PG

(Sung et al., 2024)

dendritic tree | P-stage, with Scer\GAL4^ppk.PU

(Zhang et al., 2014)

embryo | dorsal closure stage, with Scer\GAL4^69B

(Chavoshi et al., 2010)

embryo | embryonic stage 14, with Scer\GAL4^69B

(Chavoshi et al., 2010)

embryonic/larval midgut, with Scer\GAL4^da.G32

(Mohseni et al., 2009)

embryonic/larval optic lobe | larval stage, with Scer\GAL4^GMR31H09

(Lee et al., 2024)

fat body, with Scer\GAL4^Lsp2.PH

(Liu et al., 2013)

gamma Kenyon cell, with Scer\GAL4^alrm.PD

(Tasdemir-Yilmaz and Freeman, 2014)

gamma Kenyon cell | somatic clone, with Scer\GAL4^alrm.PD

(Tasdemir-Yilmaz and Freeman, 2014)

larval brain, with Scer\GAL4^GMR31H09

(Lee et al., 2024)

larval CCAP neuron | early third instar larval stage, with Scer\GAL4^CCAP.PP

(Lee et al., 2019)

larval CCAP neuron | second instar larval stage, with Scer\GAL4^CCAP.PP

(Lee et al., 2019)

larval CCAP neuron | third instar larval stage, with Scer\GAL4^Burs.PL

(Lee et al., 2019)

larval CCAP neuron | wandering third instar larval stage, with Scer\GAL4^CCAP.PP

(Lee et al., 2019)

larval central brain, with Scer\GAL4^GMR31H09

(Lee et al., 2024)

larval digestive system | larval stage, with Scer\GAL4^A9

(Lee et al., 2019)

larval dorsal multidendritic neuron ddaC, with Scer\GAL4^109(2)80

(Kirilly et al., 2009)

larval dorsal multidendritic neuron ddaC, with Scer\GAL4^ppk.1.9

(Ou et al., 2008)

larval dorsal multidendritic neuron ddaC | P-stage, with Scer\GAL4^ppk.PG

(Sung et al., 2024)

larval dorsal multidendritic neuron ddaC | P-stage, with Scer\GAL4^ppk.PU

(Zhang et al., 2014)

larval thoracic neuromere | somatic clone, with Scer\GAL4^elav-C155

(Brown and Truman, 2009)

larval tracheal system | heat sensitive, with Scer\GAL4^btl.PS

(Chavoshi et al., 2010)

leg | adult stage | somatic clone, with Scer\GAL4^elav-C155

(Brown and Truman, 2009)

leg | pupal stage, with Scer\GAL4^CCAP.PP

(Lee et al., 2019)

lysosome, with Scer\GAL4^Lsp2.PH

(Liu et al., 2013, Rusten et al., 2004)

mesothoracic ventral neurosecretory neuron & axon, with Scer\GAL4^FMRFa.PS

(Brown et al., 2006)

metathoracic ventral thoracic neurosecretory neuron & axon, with Scer\GAL4^FMRFa.PS

(Brown et al., 2006)

microtubule & dorsal multidendritic neuron ddaC | pupal stage, with Scer\GAL4^ppk.PG

(Lee et al., 2009)

midgut, with Scer\GAL4^twi.PG

(Chavoshi et al., 2010)

motor neuron | adult stage | somatic clone, with Scer\GAL4^elav-C155

(Brown and Truman, 2009)

neuroblast | larval stage | somatic clone, with Scer\GAL4^elav-C155

(Brown and Truman, 2009)

neuroblast | pupal stage | somatic clone, with Scer\GAL4^elav-C155

(Brown and Truman, 2009)

neurohemal organ, with Scer\GAL4^FMRFa.PS

(Brown et al., 2006)

neuron & ventral nerve cord, with Scer\GAL4^Crz.PC

(Choi et al., 2006)

oocyte | oogenesis stage S10B | somatic clone, with Scer\GAL4^e22c

(Boyle and Berg, 2009)

peptidergic neuron | pupal stage, with Scer\GAL4^Crz.PU

(Lee et al., 2019)

prothoracic ventral neurosecretory neuron & axon, with Scer\GAL4^FMRFa.PS

(Brown et al., 2006)

synapse, with Scer\GAL4^alrm.PD

(Tasdemir-Yilmaz and Freeman, 2014)

ventral denticle belt, with Scer\GAL4^69B

(Chavoshi et al., 2010)

wing | pupal stage, with Scer\GAL4^CCAP.PP

(Lee et al., 2019)

Detailed Description

Statement

Reference

Expressing EcR^{B1-ΔC655.W650A.UAS} under the control of Scer\GAL4^CCAP.PP leads to the progressive loss of most CCAP neurons starting from late 2nd instar through to wandering third instar larval stage. This results in a severely defective pupal development, characterized by a failure in head eversion and shortened wings and legs.

Expressing EcR^{B1-ΔC655.W650A.UAS} under the control of Scer\GAL4^Crz.PU completely suppresses the death of vCrz peptidergic neurons at 6-7 hours after puparium formation.

Expressing EcR^{B1-ΔC655.W650A.UAS} under the control of Scer\GAL4^A9 results in the formation of melanotic spots in the digestive system of dying larvae.

Expressing EcR^{B1-ΔC655.W650A.UAS} under the control of Scer\GAL4^Burs.PL results in the loss of most CCAP neurons in third instar larvae.

(Lee et al., 2019)

Expression of EcR^{B1-ΔC655.W650A.Scer\UAS} in astrocytes, under the control of Scer\GAL4^alrm.PD, does not affect astrocyte morphology in 3rd instar larvae. However, EcR^{B1-ΔC655.W650A.Scer\UAS} expression completely blocks their morphological transformation into the highly vacuolated morphology apparent in controls at 6 hours after pupal formation.

Astrocyte morphological transformation can be suppressed when EcR^{B1-ΔC655.W650A.Scer\UAS} is expressed in one to two astrocytes, using MARCM, rather than the entire population. EcR^{B1-ΔC655.W650A.Scer\UAS} expression blocks the induction of phagolysomal activity.

Blockage of EcR signaling in astrocytes, through expression of EcR^{B1-ΔC655.W650A.Scer\UAS} under the control of Scer\GAL4^alrm.PD, suppresses the clearance of brp[+] synapses from multiple brain regions at 18 hours after pupal formation and vCrz[+] neuronal debris, with neurite debris lingering until 18 hours after pupal formation.

Astrocytic EcR^{B1-ΔC655.W650A.Scer\UAS} expression, under the control of Scer\GAL4^alrm.PD, blocks the transformation of these astrocytes into phagocytes.

While the dorsal and medial lobes of mushroom body γ neurons are largely pruned by 18 hours after pupal formation in controls, pruning of these axonal branches is strongly inhibited by astrocytic EcR^{B1-ΔC655.W650A.Scer\UAS} expression.

(Tasdemir-Yilmaz and Freeman, 2014)

In comparison to wild-type white prepupae, fewer and smaller autolysosomes are present in the fat body cells of flies expressing EcR^{B1-ΔC655.W650A.Scer\UAS} under the control of Scer\GAL4^Lsp2.PH.

Autophagy is significantly decreased by EcR^{B1-ΔC655.W650A.Scer\UAS} overexpression.

Expression of EcR^{B1-ΔC655.W650A.Scer\UAS} in the remodelling fat body, under the control of Scer\GAL4^Lsp2.PH delays pupal development by more than 8 hours.

(Liu et al., 2013)

Severing of the dendrites of ddaC neurons occurs in animals expressing EcR^{B1-ΔC655.W650A.Scer\UAS} under the control of Scer\GAL4^repo does occur, albeit with some delay. However, the glial membranes that wrap the proximal dendrites as part of this dendrite pruning process show very little sign of retraction or disintegration by 16 hours after puparium formation and the wrapped dendrite segments remain intact. This defect persist until the death of the neurons during metamorphosis.

(Han et al., 2011)

Expression of EcR^{B1-ΔC655.W650A.Scer\UAS} under the control of Scer\GAL4^twi.PG results in embryos with arrested midgut morphogenesis (midgut constriction and folding is impeded), while tracheal development, head involution and dorsal closure are unaffected.

Expression of EcR^{B1-ΔC655.W650A.Scer\UAS} under the control of Scer\GAL4^btl.PS at 20[o]C results in embryos with bloated dorsal tracheal trunks. If the temperature is raised to 29[o]C at the time of the 20-hydroxy-ecdysone pulse, the phenotype is more severe, with impaired tracheal branch growth.

Embryos expressing EcR^{B1-ΔC655.W650A.Scer\UAS} under the control of Scer\GAL4^69B show incomplete head involution and dorsal closure, as well as a reduced head skeleton and denticle belts.

(Chavoshi et al., 2010)

Follicular cells expressing EcR^{B1-ΔC655.W650A.Scer\UAS} under the control of Scer\GAL4^e22c have normal cell shape at S10A. Cells are smaller than normal by S10B, and no apical constriction occurs. Cell nuclei are slightly smaller compared to control cells.

Follicular cells expressing EcR^{B1-ΔC655.W650A.Scer\UAS} under the control of Scer\GAL4^e22c block stretch cell formation.

(Boyle and Berg, 2009)

Neuroblasts are found 24hr after puparium formation in 18 out of 101 MARCM clones expressing EcR^{B1-ΔC655.W650A.Scer\UAS}, in contrast to controls which show no neuroblasts at this stage.

Motorneuron clones of a laterally located lineage (identified as lineage 15) expressing EcR^{B1-ΔC655.W650A.Scer\UAS} show abnormally clumped and reduced dendritic arbors that leave large spaces devoid of dendrites. The axons of these clones generally show missing arbors in the adult femur and tibia, misaligned and clumped neuromuscular junctions, and about half the number of femoral endplates of controls. In all seven clones examined, proximal femoral branches were clumped or misaligned showing a star-shaped morphology projecting away from the primary tract. In approximately half the clones examined, the distal femoral arbors are completely missing, but of those still present most are misaligned. In a second, more medially located neuroblast clone (identified as lineage 7), similar phenotypes are seen with clumping and severe reduction in the extent of the dendritic arbor.

In the larval first thoracic neuromere, 9 of 11 clones of the neuroblast lineage identified as lineage 7, expressing EcR^{B1-ΔC655.W650A.Scer\UAS}, the ipsilateral arbors of the secondary projections clump together to produce dense strands interspersed with areas containing no bundles. The midline crossing primary bundle also defasciculated in five out of the 11 clones, and the contralateral arbor shows clumping. Lineage 7 neuroblast clones of the larval third thoracic neuromere overexpressing EcR^{B1-ΔC655.W650A.Scer\UAS} shows some abnormal fasciculation of ipsilateral and contralateral arbors.

Of five clones of lineage 6 neuroblast in the first or the third larval thoracic neuromere, expressing EcR^{B1-ΔC655.W650A.Scer\UAS}, one had a clumped ipsilateral arbor, and two showed dorsal projecting neurons with both reduced and clumped ipsilateral arbors.

Three of four dorsally located lineage 18 neuroblast clones expressing EcR^{B1-ΔC655.W650A.Scer\UAS} show a reduced ipsilateral arbor, with two of these showing clumping together of the terminal branching.

Seven of fourteen clones of lineage 9 neuroblast clones expressing expressing EcR^{B1-ΔC655.W650A.Scer\UAS} show defasciculation of the primary bundle as it curves ventrally around the leg neuropil, compared to one out of six control clones.

(Brown and Truman, 2009)

Expression of EcR^{B1-ΔC655.W650A.Scer\UAS} in ddaC neurons under the control of Scer\GAL4^109(2)80 restricts ddaC dendritic pruning resulting in excess primary and secondary dendrites remaining attached to soma at 18h APF.

(Kirilly et al., 2009)

There is no sign of microtubule disruption in the dendrites of ddaC neurons at 6 hours after puparium formation (APF) in animals expressing EcR^{B1-ΔC655.W650A.Scer\UAS} under the control of Scer\GAL4^ppk.PG (microtubules in the dendrites of wild-type ddaC neurons show breakage at this stage).

(Lee et al., 2009)

Scer\GAL4^da.G32-mediated expression of EcR^{B1-ΔC655.W650A.Scer\UAS} results in a mild persistent amnioserosa phenotype. Development of the midgut is also inhibited, but a coherent amnioserosa tissue is evident in innervated and contractile embryos, suggesting amnioserosa degeneration is indeed delayed.

(Mohseni et al., 2009)

Expression of EcR^{B1-ΔC655.W650A.Scer\UAS} under the control of Scer\GAL4^da.Switch.PT in the presence of 1μg ml[-1] RU486 in male flies results in a 10% increase in the median lifespan compared to controls. Higher levels of EcR^{B1-ΔC655.W650A.Scer\UAS} expression (using increasing concentrations of RU486) abolish this positive effect and even result in a decrease in median lifespan (14% decreased compared to controls at a concentration of 50μg ml[-1] RU486).

Expression of EcR^{B1-ΔC655.W650A.Scer\UAS} under the control of Scer\GAL4^da.Switch.PT in the presence of RU486 in female flies decreases lifespan in a RU486 dose-dependent manner.

(Tricoire et al., 2009)

Expression of EcR^{B1-ΔC655.W650A.Scer\UAS} under the control of Scer\GAL4^ppk.1.9 results in a significant reduction in the mean density of the dendrites of the ddaC dendritic arborisation neurons compared to controls. The higher order dendritic branches are primarily affected.

(Ou et al., 2008)

Targeted expression of EcR^{B1-ΔC655.W650A.Scer\UAS} driven by Scer\GAL4^αTub84B.PP abrogates metamorphosis of the CSD interneuron (CSDn), resulting in a 'larva-like' morphology.

(Roy et al., 2007)

Thoracic ventral neurosecretory cells that express EcR^{B1-ΔC655.W650A.Scer\UAS} under the control of Scer\GAL4^Fmrf.PS are of normal size and larval morphology at the start of metamorphosis. However, these mutants show defective neuronal remodelling during metamorphosis. The axons show much lower levels of pruning compared to wild-type cells and show no filopodia during the pruning phase. Scer\GAL4^Fmrf.PS>EcR^{B1-ΔC655.W650A.Scer\UAS} neurons fail to extend filopodia during the outgrowth phase. The rare filopodial-like structures that form are very short, stubby and less active than those seen on control cells. Effects on adult axon outgrowth are severe, with arbors significantly reduced in size.

(Brown et al., 2006)

Expression of EcR^{B1-ΔC655.W650A.Scer\UAS} under the control of Scer\GAL4^Crz blocks programmed cell death in Crz-expressing ventral nerve cord neurons up to 12 hours after pupal formation.

(Choi et al., 2006)

When expression is governed by Scer\GAL4^Lsp2.PH in the larval fat body early autophagosomal structures are able to form but do not acidify, perhaps due to a failure to fuse with lysosomes. At the wandering third instar stage the cytoplasm lacks the usual autophagosomes and large 2-5 micron autolysosomes, and instead contain many small structures - probably autolysosome related. Autophagic area is markedly reduced. Autophagy has been inhibited. In clones, smaller and fewer acidic structures are evident than in wild type, and fat droplets appear distinct.

(Rusten et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^CCAP.PP has visible | pupal stage phenotype, suppressible by BacA\p35^UAS.cUa, Scer\GAL4^CCAP.PP

(Lee et al., 2019)

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^CCAP.PP has abnormal size | pupal stage phenotype, suppressible by BacA\p35^UAS.cUa, Scer\GAL4^CCAP.PP

(Lee et al., 2019)

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^CCAP.PP has increased cell death | wandering third instar larval stage phenotype, suppressible by BacA\p35^UAS.cUa, Scer\GAL4^CCAP.PP

(Lee et al., 2019)

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^CCAP.PP has abnormal neuroanatomy | wandering third instar larval stage phenotype, suppressible by BacA\p35^UAS.cUa, Scer\GAL4^CCAP.PP

(Lee et al., 2019)

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^CCAP.PP has increased cell death | third instar larval stage phenotype, suppressible by rpr^RNAi.RHG.UAS, Scer\GAL4^CCAP.PP

(Lee et al., 2019)

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^CCAP.PP has increased cell death | third instar larval stage phenotype, suppressible by hid^RNAi.RHG.UAS, Scer\GAL4^CCAP.PP

(Lee et al., 2019)

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^CCAP.PP has increased cell death | third instar larval stage phenotype, suppressible by grim^RNAi.RHG.UAS, Scer\GAL4^CCAP.PP

(Lee et al., 2019)

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^CCAP.PP has increased cell death | third instar larval stage phenotype, suppressible by grim^RNAi.UAS.cLa, Scer\GAL4^CCAP.PP

(Lee et al., 2019)

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^CCAP.PP has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by rpr^RNAi.RHG.UAS, Scer\GAL4^CCAP.PP

(Lee et al., 2019)

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^CCAP.PP has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by hid^RNAi.RHG.UAS, Scer\GAL4^CCAP.PP

(Lee et al., 2019)

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^CCAP.PP has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by grim^RNAi.RHG.UAS, Scer\GAL4^CCAP.PP

(Lee et al., 2019)

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^CCAP.PP has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by grim^RNAi.UAS.cLa, Scer\GAL4^CCAP.PP

(Lee et al., 2019)

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^Burs.PL has increased cell death | third instar larval stage phenotype, suppressible | partially by Diap1^UAS.cHa, Scer\GAL4^Burs.PL

(Lee et al., 2019)

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^Burs.PL has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by Diap1^UAS.cHa, Scer\GAL4^Burs.PL

(Lee et al., 2019)

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^ppk.PU has abnormal neuroanatomy | P-stage phenotype, suppressible | partially by Nrg^HMS01638, Scer\GAL4^ppk.PU

(Zhang et al., 2014)

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^109(2)80 has abnormal neuroanatomy phenotype, suppressible by Sox14^fl.UAS, Scer\GAL4^109(2)80

(Kirilly et al., 2009)

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^109(2)80 has abnormal neuroanatomy phenotype, suppressible | partially by Mical^UAS.fl, Scer\GAL4^109(2)80

(Kirilly et al., 2009)

NOT suppressed by

Statement

Reference

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^CCAP.PP has increased cell death | third instar larval stage phenotype, non-suppressible by hid⁰⁵⁰¹⁴/Df(3L)X14

(Lee et al., 2019)

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^CCAP.PP has increased cell death | third instar larval stage phenotype, non-suppressible by rpr⁸⁷/rpr⁸⁷

(Lee et al., 2019)

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^CCAP.PP has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by hid⁰⁵⁰¹⁴/Df(3L)X14

(Lee et al., 2019)

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^CCAP.PP has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by rpr⁸⁷/rpr⁸⁷

(Lee et al., 2019)

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^109(2)80 has abnormal neuroanatomy phenotype, non-suppressible by Mical^N-ter.UAS, Scer\GAL4^109(2)80

(Kirilly et al., 2009)

NOT Suppressor of

Statement

Reference

EcR^{B1-ΔC655.W650A.UAS}/Scer\GAL4^ey-OK107 is a non-suppressor of abnormal neuroanatomy | somatic clone | progressive phenotype of bsk^flp147E

(Rallis et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^CCAP.PP has larval CCAP neuron | third instar larval stage phenotype, suppressible by rpr^RNAi.RHG.UAS, Scer\GAL4^CCAP.PP

(Lee et al., 2019)

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^CCAP.PP has larval CCAP neuron | third instar larval stage phenotype, suppressible by hid^RNAi.RHG.UAS, Scer\GAL4^CCAP.PP

(Lee et al., 2019)

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^CCAP.PP has larval CCAP neuron | third instar larval stage phenotype, suppressible by grim^RNAi.UAS.cLa, Scer\GAL4^CCAP.PP

(Lee et al., 2019)

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^Burs.PL has larval CCAP neuron | third instar larval stage phenotype, suppressible | partially by Diap1^UAS.cHa, Scer\GAL4^Burs.PL

(Lee et al., 2019)

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^CCAP.PP has leg | pupal stage phenotype, suppressible by BacA\p35^UAS.cUa, Scer\GAL4^CCAP.PP

(Lee et al., 2019)

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^CCAP.PP has wing | pupal stage phenotype, suppressible by BacA\p35^UAS.cUa, Scer\GAL4^CCAP.PP

(Lee et al., 2019)

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^CCAP.PP has larval CCAP neuron | wandering third instar larval stage phenotype, suppressible by BacA\p35^UAS.cUa, Scer\GAL4^CCAP.PP

(Lee et al., 2019)

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^ppk.PU has larval dorsal multidendritic neuron ddaC | P-stage phenotype, suppressible | partially by Nrg^HMS01638, Scer\GAL4^ppk.PU

(Zhang et al., 2014)

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^ppk.PU has dendritic tree | P-stage phenotype, suppressible | partially by Nrg^HMS01638, Scer\GAL4^ppk.PU

(Zhang et al., 2014)

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^109(2)80 has dendrite phenotype, suppressible by Sox14^fl.UAS, Scer\GAL4^109(2)80

(Kirilly et al., 2009)

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^109(2)80 has larval dorsal multidendritic neuron ddaC phenotype, suppressible by Sox14^fl.UAS, Scer\GAL4^109(2)80

(Kirilly et al., 2009)

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^109(2)80 has dendrite phenotype, suppressible | partially by Mical^UAS.fl, Scer\GAL4^109(2)80

(Kirilly et al., 2009)

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^109(2)80 has larval dorsal multidendritic neuron ddaC phenotype, suppressible | partially by Mical^UAS.fl, Scer\GAL4^109(2)80

(Kirilly et al., 2009)

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^Lsp2.PH has lysosome phenotype, suppressible by Pten^UAS.cGb, Scer\GAL4^Lsp2.PH

(Rusten et al., 2004)

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^Lsp2.PH has autophagosome phenotype, suppressible by Pten^UAS.cGb, Scer\GAL4^Lsp2.PH

(Rusten et al., 2004)

NOT suppressed by

Statement

Reference

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^CCAP.PP has larval CCAP neuron | third instar larval stage phenotype, non-suppressible by hid⁰⁵⁰¹⁴/Df(3L)X14

(Lee et al., 2019)

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^CCAP.PP has larval CCAP neuron | third instar larval stage phenotype, non-suppressible by rpr⁸⁷/rpr⁸⁷

(Lee et al., 2019)

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^109(2)80 has dendrite phenotype, non-suppressible by Mical^N-ter.UAS, Scer\GAL4^109(2)80

(Kirilly et al., 2009)

EcR^{B1-ΔC655.W650A.UAS}, Scer\GAL4^109(2)80 has larval dorsal multidendritic neuron ddaC phenotype, non-suppressible by Mical^N-ter.UAS, Scer\GAL4^109(2)80

(Kirilly et al., 2009)

NOT Suppressor of

Statement

Reference

EcR^{B1-ΔC655.W650A.UAS}/Scer\GAL4^ey-OK107 is a non-suppressor of alpha/beta Kenyon cell phenotype of bsk^flp147E

(Rallis et al., 2013)

EcR^{B1-ΔC655.W650A.UAS}/Scer\GAL4^ey-OK107 is a non-suppressor of gamma Kenyon cell phenotype of bsk^flp147E

(Rallis et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Overexpression of drpr^I.Scer\UAS (under the control of Scer\GAL4^repo) in a EcR^{B1-ΔC655.W650A.Scer\UAS} background fails to transform astrocytes into phagocytes at puparium formation.

(Tasdemir-Yilmaz and Freeman, 2014)

Co-expression of Sox14^fl.Scer\UAS with EcR^{B1-ΔC655.W650A.Scer\UAS} in ddaC neurons under the control of Scer\GAL4^109(2)80 suppresses the excess dendritic arborization found upon expression of EcR^{B1-ΔC655.W650A.Scer\UAS} alone in ddaC neurons under the control of Scer\GAL4^109(2)80.

Co-expression of Mical^Scer\UAS.fl with EcR^{B1-ΔC655.W650A.Scer\UAS} in ddaC neurons under the control of Scer\GAL4^109(2)80 partially suppresses the excess dendritic arborization found upon expression of EcR^{B1-ΔC655.W650A.Scer\UAS} alone in ddaC neurons under the control of Scer\GAL4^109(2)80.

Co-expression of Mical^{N-ter.Scer\UAS} with EcR^{B1-ΔC655.W650A.Scer\UAS} in ddaC neurons under the control of Scer\GAL4^109(2)80 fails to affect the excess dendritic arborization found upon expression of EcR^{B1-ΔC655.W650A.Scer\UAS} alone in ddaC neurons under the control of Scer\GAL4^109(2)80.

(Kirilly et al., 2009)

The deleterious effect on female lifespan of expression of EcR^{B1-ΔC655.W650A.Scer\UAS} under the control of Scer\GAL4^da.Switch.PT in the presence of RU486 is suppressed by ovo^D1.

(Tricoire et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

EcR^{B1-ΔC655.W650A.UAS} is rescued by EcR^B1.UAS/Scer\GAL4^CCAP.PP

(Lee et al., 2019)

EcR^{B1-ΔC655.W650A.UAS} is rescued by Scer\GAL4^CCAP.PP/EcR^B2.UAS

(Lee et al., 2019)

EcR^{B1-ΔC655.W650A.UAS} is rescued by Scer\GAL4^twi.PG/EcR^A.UAS

(Chavoshi et al., 2010)

EcR^{B1-ΔC655.W650A.UAS} is rescued by Scer\GAL4^btl.PS/EcR^A.UAS

(Chavoshi et al., 2010)

EcR^{B1-ΔC655.W650A.UAS} is rescued by Scer\GAL4^69B/EcR^A.UAS

(Chavoshi et al., 2010)

EcR^{B1-ΔC655.W650A.UAS} is rescued by Scer\GAL4^109(2)80/EcR^B1.UAS

(Kirilly et al., 2009)

Partially rescued by

EcR^{B1-ΔC655.W650A.UAS} is partially rescued by Scer\GAL4^CCAP.PP/EcR^A.UAS

(Lee et al., 2019)

Comments

Expressing either EcR^B1.UAS or EcR^B1.UAS under the control of Scer\GAL4^CCAP.PP fully rescues CCAP neuronal death in larvae and the pupal phenotype (failure in head eversion and shortened wings and legs) induced by expression of EcR^{B1-ΔC655.W650A.UAS} alone. Expressing EcR^A.UAS results only in a partial rescue of both larval CCAP neuronal death and the reduced size of pupal legs and wings, accompanied by a full rescue of the head eversion phenotype.

(Lee et al., 2019)

The defects in midgut morphogenesis which are seen in embryos expressing EcR^{B1-ΔC655.W650A.Scer\UAS} under the control of Scer\GAL4^twi.PG are rescued by co-expression of EcR^A.Scer\UAS.

The defects in tracheal morphogenesis which are seen in embryos expressing EcR^{B1-ΔC655.W650A.Scer\UAS} under the control of Scer\GAL4^btl.PS are rescued by co-expression of EcR^A.Scer\UAS.

The embryonic defects caused by expression or EcR^{B1-ΔC655.W650A.Scer\UAS} under the control of Scer\GAL4^69B are rescued by co-expression of EcR^A.Scer\UAS.

(Chavoshi et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

6872

w¹¹¹⁸; P{UAS-EcR.B1-ΔC655.W650A}TP1-9

Notes on Origin

Discoverer

Comments

Encodes a dominant negative form of EcR.

(Cherbas et al., 2003)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

EcR^{B1-ΔC655.W650A.Scer\UAS}

EcR^{B1-ΔC655.W650A.UAS}

EcR^DN

(Williams and Truman, 2005)

EcR^W650A

(Lee et al., 2019, Gautam and Tapadia, 2010, Okamoto et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (62)

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