FlyBase Allele Report: Dmel\Apc2[UAS.GFP]

General Information

Symbol

Dmel\Apc2^UAS.GFP

Species

D. melanogaster

Name

FlyBase ID

FBal0141949

Feature type

allele

Associated gene

Dmel\Apc2

Associated Insertion(s)

Carried in Construct

P{UAS-Apc2.GFP}

Also Known As

UAS-APC2-GFP, Apc2-GFP

Key Links

Transgenic product class

wild_type

(Akong et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Akong et al., 2002)

Mutagen

in vitro construct

(Akong et al., 2002, Akong et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-Apc2.GFP}

Cytology

Description

UAS regulatory sequences drive expression of Apc2 tagged with GFP.

(Akong et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Apc2

Tagged with

GFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable, with Scer\GAL4^pros.PMG

(Akong et al., 2002)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Apc2^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^pros.PMG has no effect on overall brain morphology.

(Akong et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Apc2^UAS.GFP, Scer\GAL4^wor.PA is a suppressor of abnormal neuroanatomy | larval stage phenotype of Scer\GAL4^wor.PA, klu^UAS.Tag:HA

(Komori et al., 2014)

Scer\GAL4^{VP16.nanos.UTR}/Apc2^UAS.GFP is a suppressor of decreased cell number phenotype of chic⁰¹³²⁰/chic¹¹

(Shields et al., 2014)

NOT Suppressor of

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}/Apc2^UAS.GFP is a non-suppressor of decreased cell number phenotype of chic⁰¹³²⁰/chic²²¹

(Shields et al., 2014)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Apc2^UAS.GFP/Scer\GAL4^da.G32 is a non-enhancer of ventral denticle belt | germline clone phenotype of sgg^M11

(Tolwinski, 2009)

Apc2^UAS.GFP/Scer\GAL4^da.G32 is a non-enhancer of ventral denticle belt | germline clone phenotype of Axn^S044230

(Tolwinski, 2009)

Apc2^UAS.GFP/Scer\GAL4^da.G32 is a non-enhancer of ventral denticle belt | germline clone phenotype of Apc2^d40, Apc^Q8, Axn^S044230

(Tolwinski, 2009)

Suppressor of

Statement

Reference

Apc2^UAS.GFP, Scer\GAL4^wor.PA is a suppressor of type II neuroblast | increased number phenotype of Scer\GAL4^wor.PA, klu^UAS.Tag:HA

(Komori et al., 2014)

Scer\GAL4^{VP16.nanos.UTR}/Apc2^UAS.GFP is a suppressor of male germline stem cell phenotype of chic⁰¹³²⁰/chic¹¹

(Shields et al., 2014)

Apc2^UAS.GFP/Scer\GAL4^da.G32 is a suppressor of ventral denticle belt | germline clone phenotype of Apc2^d40, Apc^Q8

(Tolwinski, 2009)

NOT Suppressor of

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}/Apc2^UAS.GFP is a non-suppressor of male germline stem cell phenotype of chic⁰¹³²⁰/chic²²¹

(Shields et al., 2014)

Apc2^UAS.GFP/Scer\GAL4^da.G32 is a non-suppressor of ventral denticle belt | germline clone phenotype of Apc2^d40, Apc^Q8, Axn^S044230

(Tolwinski, 2009)

Apc2^UAS.GFP/Scer\GAL4^da.G32 is a non-suppressor of ventral denticle belt | germline clone phenotype of Axn^S044230

(Tolwinski, 2009)

Apc2^UAS.GFP/Scer\GAL4^da.G32 is a non-suppressor of ventral denticle belt | germline clone phenotype of sgg^M11

(Tolwinski, 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The formation of supernumerary neuroblasts which is seen in clones derived from single type II neuroblasts expressing klu^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^wor.PA is suppressed if the clones are also co-expressing Apc2^{Scer\UAS.T:Avic\GFP}.

(Komori et al., 2014)

Expression of Apc2^{Scer\UAS.T:Avic\GFP} in germ cells under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} suppresses the germline stem cell (GSC) loss seen in chic¹¹/chic⁰¹³²⁰ mutant testes.

Expression of Apc2^{Scer\UAS.T:Avic\GFP} in germ cells under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} does not suppress the germline stem cell (GSC) loss seen in chic²²¹/chic⁰¹³²⁰ mutant testes.

(Shields et al., 2014)

Expression of Apc2^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^da.G32 has no effect on the ventral denticle belt phenotype of the germ-line and zygotic Axn^S044230-mutation.

Expression of Apc2^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^da.G32 restores wild-type patterning of the ventral cuticle in Apc^Q8, Apc2^d40 double mutant embryos.

Expression of Apc2^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^da.G32 has no effect on the ventral denticle belt phenotype of Axn^S044230, Apc^Q8, Apc2^d40 triple mutants.

Expression of Apc2^{Scer\UAS.T:Avic\GFP} has no effect on the ventral denticle belt phenotype of sgg^M11 mutant embryos.

(Tolwinski, 2009)

Expression of both Apc^Scer\UAS.cHa and Apc2^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^pros.PMG has no effect on overall brain morphology.

(Akong et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Apc2^UAS.GFP/Scer\GAL4^arm.PS partially rescues Apc2^N175K

(Hamada and Bienz, 2002)

Comments

Expression of Apc2^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^arm.PS rescues Apc2^N175K flies through to adulthood, although their viability is poor.

(Hamada and Bienz, 2002)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

8815

w^*; P{UAS-Apc2.GFP}3/TM3, Sb¹

Notes on Origin

Discoverer

R. Rosin-Arbesfeld M. Bienz

(Akong et al., 2002, Akong et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Apc2^{Scer\UAS.T:Avic\GFP}

Apc2^UAS.GFP

Name Synonyms

Secondary FlyBase IDs

References (19)

Report Sections

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