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General Information
Symbol
Dmel\Sod2n283
Species
D. melanogaster
Name
FlyBase ID
FBal0144560
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Comment:

Reported as a 167 bp deletion in Sod2 resulting from the imprecise excision of P{SUPor-P}Sod2KG06854, extending from 2R:11837445..11837611 in R3 coordinates.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Cytology
Nature of the lesion
Statement
Reference

167bp deletion that removes part of the first exon and intron of Sod2 (coordinates 2R:11837612..11837444 , release 3 genome).

Imprecise excision of the insertion in Sod2KG06854, resulting in a deletion of a large portion of the 3' end of the inserted element and some of the Sod2 gene. The 5' end of the inserted element is unaffected.

Insertion components
P{5'SUPor-P}Sod2n283
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Both the median and maximum lifespan of Sod2n283/Sod2KG06854 flies are shortened by approximately 30% compared to wild-type controls.

40- to 42-day old Sod2n283/Sod2KG06854 adults show no increase in the mtDNA mutation frequency compared to controls.

Sod2n283 heterozygous flies have normal lifespans.

Odor avoidance in homozygous adults is indistinguishable from that of controls at 3 and 8 hours of age, but by 16 hours of age olfactory behaviour of homozygotes is no longer detectable (in contrast to control flies).

Negative geotaxis behaviour is substantially impaired in homozygous adults by 2 hours of age and declines to nearly undetectable levels by 7 hours of age.

At 3 hours of age, homozygous adults have a 22% lower resting heart rate than controls. The mutant adults have a more variable resting heart rate throughout the first 24 hours of adult life than controls, which remains on average, much slower than normal. This slower heart rate is due to frequent pauses, during which the mutant heart fails to initiate productive contractions. However, when the heartbeat resumes following a pause, it tends to beat more quickly in the mutant flies. The contractile velocity of the heart in homozygous adults is not significantly different from that of controls over the first 24 hours of adult life. The relaxation velocity of the heart declines steadily in homozygous adults over the first 24 hours of adult life, while that of controls remains steady.

Homozygous adults show a clear trend for spontaneous ambulatory movement to begin to decline immediately within the first 3 hours, and then to be completely absent in most mutant flies by 12 hours of age. There is a decline in the number of mutant flies that respond to gentle stimulus with movement, starting at 9 hours of age and continuing throughout the time course of the experiment (to 24 hours of age), while wild-type flies do not show this decline. Some mutant adults have lost a detectable heartbeat by 6 hours of age, and the number of mutant flies with functional, beating hearts declines thereafter throughout the time course of the experiment (to 24 hours of age).

Chronic hypoxia (5% O[[2]]) partially restores the abbreviated life span of mutant flies.

Sod2n283/+ mutant adult brains do not exhibit neurodegeneration or increased apoptosis of the cortex and neuropil, as compared with controls.

Mean and maximum lifespan are reduced by 20-24% in heterozygous flies. The flies show increased age-specific mortality.

Mean and maximum lifespan are reduced by 38% and 43% respectively in Sod2n283/Sod2KG06854 flies. The flies show increased age-specific mortality.

The brains of homozygous adults show obvious TUNEL staining within the first 20 hours of eclosion, in contrast to wild-type controls. Obvious vacuoles are seen in the brain by 29 days of age (in wild-type brains, neuronal loss is not seen at 35 days of age).

Odor avoidance declines more rapidly with age in Sod2n283/Sod2KG06854 flies than in wild-type controls.

Approximately 98% of homozygous adults die within 24 hours of eclosion and by 36 hours after eclosion all the homozygotes have died.

Heterozygotes are approximately twice as sensitive to oxidative stress (treatment with paraquat) compared to wild-type flies.

Homozygous adults die within 24 hours of eclosion. Heterozygotes have a reduced average life-span of 22-24 days and are slightly more sensitive to paraquat treatment than wild-type controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Transheterozygous Sod2n283 p38bΔ45 mutants have significantly reduced lifespans compared to controls.

Xenogenetic Interactions
Statement
Reference

Sod2n283 heterozygotes expressing Hsap\MAPTE14.Scer\UAS under the control of Scer\GAL4elav.PU are short lived as compared to controls.

Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
References (14)