FlyBase Allele Report: Dmel\Parp[GMR.PU]

General Information

Symbol

Dmel\Parp^GMR.PU

Species

D. melanogaster

Name

glass multimer reporter construct of Uchida

FlyBase ID

FBal0144696

Feature type

allele

Associated gene

Dmel\Parp

Associated Insertion(s)

Carried in Construct

P{GMR-Parp.U}

Allele class

Mutagen

in vitro construct - regulatory fusion

Nature of the Allele

Allele class

Mutagen

in vitro construct - regulatory fusion

(Uchida et al., 2002)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{GMR-Parp.U}

Cytology

Nature of the lesion

Statement

Reference

GMR regulatory sequences drive expression of a full length Parp cDNA.

(Uchida et al., 2002)

Allele components

Product class / Tool use(s)

Regulatory region(s)

GMR

engineered transcription regulatory region

Encoded product / tool

Parp

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

increased cell death

(Uchida et al., 2002)

planar polarity defective

(Uchida et al., 2002)

visible

(Uchida et al., 2002)

Phenotype Manifest In

actin filament & retina

(Uchida et al., 2002)

cone cell

(Uchida et al., 2002)

eye

(Uchida et al., 2002)

eye disc

(Uchida et al., 2002)

ommatidial precursor cluster

(Uchida et al., 2002)

ommatidium

(Uchida et al., 2002)

photoreceptor cell R3

(Uchida et al., 2002)

photoreceptor cell R4

(Uchida et al., 2002)

pigment cell

(Uchida et al., 2002)

Detailed Description

Statement

Reference

Flies expressing Parp^GMR.PU have a rough eye phenotype. Ommatidia have an abnormal shape and size and ommatidial fusion is seen. The orientation of the ommatidia is disrupted; some ommatidia are rotated incorrectly and show incorrect chiral forms. A rectangular pattern of rhabdomeres is seen in some flies. The number of non-neuronal accessory cells in the eye is almost normal, although their morphology and arrangement is abnormal. The arrangement of photoreceptor clusters is slightly disordered in thid larval instar eye discs expressing Parp^GMR.PU. R3/R4 cells are arranged in random orientation in each ommatidium, indicating disordered polarity. The number of apoptotic cells posterior to the morphogenetic furrow is slightly higher than normal. DNA synthesis and mitosis appear normal in these discs. The organisation of the F-actin cytoskeleton is disrupted in the eyes of 40 hour pupae expressing Parp^GMR.PU; F-actin is sporadically organized at the apical surface and the spokewise pattern that is normally seen at the apical surface is almost completely absent. The normal accumulation of F-actin at the centre of the ommatidium (which is the precursor of the rhabdomere) is sometimes missing and the arrangement of the secondary and tertiary pigment cells and bristle complexes at the basal floor is also disrupted.

(Uchida et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Parp^GMR.PU has planar polarity defective phenotype, non-suppressible by BacA\p35^GMR.PH

(Uchida et al., 2002)

Suppressor of

Statement

Reference

Parp^GMR.PU is a suppressor | partially of visible phenotype of Rho1^GMR.PH

(Uchida et al., 2002)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Parp^GMR.PU has ommatidium phenotype, non-suppressible by BacA\p35^GMR.PH

(Uchida et al., 2002)

Suppressor of

Statement

Reference

Parp^GMR.PU is a suppressor | partially of ommatidium phenotype of Rho1^GMR.PH

(Uchida et al., 2002)

Parp^GMR.PU is a suppressor | partially of pigment cell phenotype of Rho1^GMR.PH

(Uchida et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

The misrotation and incorrect chirality of ommatidia seen in flies expressing Parp^GMR.PU is not suppressed by co-expression of BacA\p35^GMR.PH. Parp^GMR.PU shows no genetic interaction with fz¹/+, fz²³/+, fz^GMR.PA or dsh¹/+. The Rho1^GMR.PH phenotype is partially suppressed by Parp^GMR.PU; ommatidial lattices are seen in the rescued flies and pigment cells are seen in deeper regions of the retina. A distinct rhabdomere structure also appears.

(Uchida et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Parp^GMR.PU

Name Synonyms

glass multimer reporter construct of Uchida

(Uchida et al., 2002)

Secondary FlyBase IDs

References (1)

Report Sections

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