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General Information
Symbol
Dmel\nan36a
Species
D. melanogaster
Name
FlyBase ID
FBal0146994
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Comment:

Imprecise excision of P{lacW}519 has caused a deletion that removes bases -1540 to +575 with respect to the nan start codon.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of P{lacW}519 has caused a deletion that removes bases -1540 to +575 with respect to the nan start codon.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

nan36a larvae show decreased head-touch response.

nan36a mutant adult females have no difference in preference for softer plain, or 100 mM sucrose-containing, agarose for egg laying, compared to controls.

nan36a mutant adults have no difference in spiking or mechanotransduction currents of mechanosensory neurons of the labellar taste bristles or labellar taste pegs in response to mechanical stimuli, compared to controls.

nan36a mutant adults show defects in sensing food hardness and in contrast to wild-type display diminished preference for softer food with lower sugar content (0.5mM sucrose, 0.2% agarose) to a hard food with higher sugar content (1mM sucrose, 2% agarose) in a binary choice assay. The pharyngeal pumping rate of nan36a mutants is similar to wild-type.

nan36a mutant adults have significantly increased water consumption, compared to controls.

Mutant flies show a severe defect in negative geotaxis.

Mutant flies show significantly reduced speed and precision of movements during walking compared to controls. They have a reduced ability to successfully traverse gaps compared to wild type, even at short gap distances of 2.5mm.

nan36a mutant larvae do not have alterations in defecation rhythm compared to controls.

The gustatory aversion of mutant flies to 6mM camphor in a two way-choice test is not significantly different from that seen in wild-type flies.

Cho neurons in nan36a mutant larvae exhibit a lower spontaneous firing rate and no increase in action potential firing in response to sound. Ca[2+] imaging also reveals no response of nan36a mutant Cho neurons to sound.

Mutant flies exhibit the wild type avoidance of aristolochic acid in food choice assays.

Mutant adults show normal avoidance of 1% citronellal in a direct airborne repellent test (DART) assay.

Mutant larvae move more slowly than wild-type larvae at both 14[o] and 17.5[o]C.

The normal preference of third instar larvae for 17.5[o]C over 14[o]C in a two-way choice test is eliminated in mutant larvae if the larvae are released in the middle of the plate at the boundary between the two temperature zones. However, if the larvae are released at either end of the plate at the extreme end of either temperature zone, they mostly stay within that temperature zone and thus the defect seen when the larvae are released in the middle of the plate may be due to impaired locomotor activity.

The response of Johnston's organ neurons to arista deflection (measured by changes in intracellular Ca[2+] concentration) is largely eliminated in nan36a homozygous adults.

Compared with wild-type, nan36a mutants display impaired negative geotaxis behaviour in addition to their reduced general locomotion.

The Johnston's organ of nan36a mutant flies show impaired neural activity in response to body rotation.

nan36a mutants retain a preference for 18[o]C (i.e. are thermotactic).

Homozygous flies show disrupted hygrosensing behaviour; a higher fraction of flies choose moist air (100% humidity) over dry air (0% humidity) compared to wild-type controls in a hygrosensory behaviour assay after five minutes.

Mutants show an impaired electrophysiological response to dry air, while leaving moist air sensing unaffected (recordings were taken at the base of the 2nd antennal segment in these experiments).

Homozygotes and nandy5/nan36a transheterozygous adults are viable but show abnormal behaviour: they are sedentary, mildly uncoordinated, more likely than wild-type flies to fall whilst climbing, and slower to right themselves after a fall. Homozygotes show a loss of sound-evoked potentials when the antennal extracellular potentials are recorded. Mutant chordotonal organs appear to have normal ultrastructure.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

The defects in sensing food hardness characteristic for nan36a mutant adults can be rescued by expression of nanScer\UAS.cKa under the control of Scer\GAL4GMR41E11 in the mutant background.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
References (32)