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General Information
Symbol
Dmel\hidAla5.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0147320
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-hidAla5, UAS-HIDAla5
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of a mutated form of hid in which the 5 phosphoacceptor residues of the rl phosphorylation sites have been mutated to non-phosphorylatable Ala residues; carries the amino acid replacements S121A, T148A, T180A, T228A and S251A.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Scer\GAL4Send2.4kb driven by Scer\GAL4Send2.4kb results in the ablation of spermathecal secretory cells (SSC) due to ectopic apoptosis. By 43 hours post-mating, the majority of SSC are dead. Females whose SSC are ablated after mating using WAla5.Scer\UAS in combination with Scer\GAL4Send2.4kb retain as many sperm cells in their spermathecae as do their wild-type sisters. Sperm clumping in the seminal receptacle is not observed in these SSC-ablated females.

Expression of Scer\GAL4Send1.4kb driven by Scer\GAL4Send1.4kb results in the ablation of spermathecal secretory cells (SSC) due to ectopic apoptosis. Similarly to wild-type animals, sperm cells are found in the seminal receptacle and uterus, and occasionally in the oviduct of mated females whose SSC are ablated prior to mating. However, in contrast to wild-type controls, sperm cells are often absent in the spermathecae. By 7 hours post-mating, nearly all remaining sperm cells in wild-type females are in the seminal receptacle or spermathecae. By contrast, SSC-ablated females expressing WAla5.Scer\UAS under the control of Scer\GAL4Send1.4kb have sperm cells in their seminal receptacles, yet tend to lack sperm cells in the spermathecae.

Females with SSC ablated prior to mating as a result of Scer\GAL4Send1.4kb-driven expression of WAla5.Scer\UAS lay as many eggs on days 1 to 3 post-mating as their wild-type sisters. However, after day 3, their egg laying is significantly reduced compared with wild-type. In contrast to wild-type, these SSC-ablated females are ovoviviparous: a large proportion of such females have a late-stage embryo or live first-instar larva stuck in the uterus.

Hemocytes in WAla5.Scer\UAS; Scer\GAL4srp.Hemo late embryos aggregate abnormally.

Hemocytes in WAla5.Scer\UAS; Scer\GAL4srp.Hemo late stage embryos aggregate abnormally. The hemocytes in these animals have fewer extensions than wild-type, are dramatically enlarged and carry multiple inclusions.

Stage 17 WAla5.Scer\UAS; Scer\GAL4sli.PS embryos have little or no midline glial cells and fused commissures in the ventral nerve cord.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Wings discs containing clones coexpressing WAla5.Scer\UAS and BacA\p35Scer\UAS.cHa under the control of Scer\GAL4Act5C.PP show increased cell proliferation, with most of the increased proliferation occurring in cells outside the clones.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
WAla5.Scer\UAS
hidAla5.Scer\UAS
hidAla5.UAS
Name Synonyms
Secondary FlyBase IDs
    References (7)