Expression of Scer\GAL4Send2.4kb driven by Scer\GAL4Send2.4kb results in the ablation of spermathecal secretory cells (SSC) due to ectopic apoptosis. By 43 hours post-mating, the majority of SSC are dead. Females whose SSC are ablated after mating using WAla5.Scer\UAS in combination with Scer\GAL4Send2.4kb retain as many sperm cells in their spermathecae as do their wild-type sisters. Sperm clumping in the seminal receptacle is not observed in these SSC-ablated females.
Expression of Scer\GAL4Send1.4kb driven by Scer\GAL4Send1.4kb results in the ablation of spermathecal secretory cells (SSC) due to ectopic apoptosis. Similarly to wild-type animals, sperm cells are found in the seminal receptacle and uterus, and occasionally in the oviduct of mated females whose SSC are ablated prior to mating. However, in contrast to wild-type controls, sperm cells are often absent in the spermathecae. By 7 hours post-mating, nearly all remaining sperm cells in wild-type females are in the seminal receptacle or spermathecae. By contrast, SSC-ablated females expressing WAla5.Scer\UAS under the control of Scer\GAL4Send1.4kb have sperm cells in their seminal receptacles, yet tend to lack sperm cells in the spermathecae.
Females with SSC ablated prior to mating as a result of Scer\GAL4Send1.4kb-driven expression of WAla5.Scer\UAS lay as many eggs on days 1 to 3 post-mating as their wild-type sisters. However, after day 3, their egg laying is significantly reduced compared with wild-type. In contrast to wild-type, these SSC-ablated females are ovoviviparous: a large proportion of such females have a late-stage embryo or live first-instar larva stuck in the uterus.
Hemocytes in WAla5.Scer\UAS; Scer\GAL4srp.Hemo late stage embryos aggregate abnormally. The hemocytes in these animals have fewer extensions than wild-type, are dramatically enlarged and carry multiple inclusions.