FB2025_04 , released October 2, 2025
Allele: Dmel\hidAla5.UAS
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General Information
Symbol
Dmel\hidAla5.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0147320
Feature type
allele
Associated gene
Dmel\hid
Associated Insertion(s)
Carried in Construct
P{UAS-hid.Ala5}
Also Known As
UAS-hidAla5, UAS-HIDAla5
Key Links
Transgenic product class
missense_variant
(Bergmann et al., 2002)
polypeptide_post_translational_processing_variant
(Bergmann et al., 2002)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Bergmann et al., 2002)
polypeptide_post_translational_processing_variant
(Bergmann et al., 2002)
Mutagen
in vitro construct
(Bergmann et al., 2002)
Progenitor genotype
Carried in construct
P{UAS-hid.Ala5}
Cytology
Description

UAS regulatory sequences drive expression of a mutated form of hid in which the 5 phosphoacceptor residues of the rl phosphorylation sites have been mutated to non-phosphorylatable Ala residues; carries the amino acid replacements S121A, T148A, T180A, T228A and S251A.

(Bergmann et al., 2002)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
hid
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Scer\GAL4Send2.4kb driven by Scer\GAL4Send2.4kb results in the ablation of spermathecal secretory cells (SSC) due to ectopic apoptosis. By 43 hours post-mating, the majority of SSC are dead. Females whose SSC are ablated after mating using WAla5.Scer\UAS in combination with Scer\GAL4Send2.4kb retain as many sperm cells in their spermathecae as do their wild-type sisters. Sperm clumping in the seminal receptacle is not observed in these SSC-ablated females.

      Expression of Scer\GAL4Send1.4kb driven by Scer\GAL4Send1.4kb results in the ablation of spermathecal secretory cells (SSC) due to ectopic apoptosis. Similarly to wild-type animals, sperm cells are found in the seminal receptacle and uterus, and occasionally in the oviduct of mated females whose SSC are ablated prior to mating. However, in contrast to wild-type controls, sperm cells are often absent in the spermathecae. By 7 hours post-mating, nearly all remaining sperm cells in wild-type females are in the seminal receptacle or spermathecae. By contrast, SSC-ablated females expressing WAla5.Scer\UAS under the control of Scer\GAL4Send1.4kb have sperm cells in their seminal receptacles, yet tend to lack sperm cells in the spermathecae.

      Females with SSC ablated prior to mating as a result of Scer\GAL4Send1.4kb-driven expression of WAla5.Scer\UAS lay as many eggs on days 1 to 3 post-mating as their wild-type sisters. However, after day 3, their egg laying is significantly reduced compared with wild-type. In contrast to wild-type, these SSC-ablated females are ovoviviparous: a large proportion of such females have a late-stage embryo or live first-instar larva stuck in the uterus.

      (Schnakenberg et al., 2011)

      Hemocytes in WAla5.Scer\UAS; Scer\GAL4srp.Hemo late embryos aggregate abnormally.

      (Bruckner et al., 2004)

      Hemocytes in WAla5.Scer\UAS; Scer\GAL4srp.Hemo late stage embryos aggregate abnormally. The hemocytes in these animals have fewer extensions than wild-type, are dramatically enlarged and carry multiple inclusions.

      (Bruckner et al., 2004)

      Stage 17 WAla5.Scer\UAS; Scer\GAL4sli.PS embryos have little or no midline glial cells and fused commissures in the ventral nerve cord.

      (Bergmann et al., 2002)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Wings discs containing clones coexpressing WAla5.Scer\UAS and BacA\p35Scer\UAS.cHa under the control of Scer\GAL4Act5C.PP show increased cell proliferation, with most of the increased proliferation occurring in cells outside the clones.

      (Huh et al., 2004)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      Bergmann

      (Bergmann et al., 2002)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      WAla5.Scer\UAS
      hidAla5.Scer\UAS
      hidAla5.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (7)