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General Information
Symbol
Dmel\slifUY681
Species
D. melanogaster
Name
FlyBase ID
FBal0151480
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
slifAnti, UAS-Slif-anti
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

The P{Mae-UAS.6.11} is inserted in the first intron of the slif gene. The orientation of the insert is opposite to the direction of transcription.

Insertion components
P{Mae-UAS.6.11}slifUY681
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Adult females expressing slifUY681 under the control of Scer\GAL4fit.PS show significantly weaker suppression of feeding (on standard food) after pre-feeding with tryptone compared to controls.

Expression of slifUY681, throughout development, under the control of Scer\GAL4ple.PF, but not Scer\GAL4NPF.PU, Scer\GAL4Trh.PB, Scer\GAL4Tdc2.PU, Scer\GAL4ChAT.PU, Scer\GAL4Gad1.3.098, Scer\GAL4Hug.PU or Scer\GAL4386Y, results in a significant reduction in larval food intake, compared to controls; this effect also occurs when a Gal80[ts] transgene is used to restrict expression under the control of Scer\GAL4ple.PF to the 16 hours prior to analysis.

Expression of slifUY681 under the control of Scer\GAL4ple.TH-D', but not Scer\GAL4ple.TH-C, in combination with a Gal80[ts] transgene to restrict expression to the 16 hours prior to analysis, reduces larval food intake and body size, compared to controls.

Central nervous system neuroblasts of fed larvae expressing slifUY681 under the control of Scer\GAL4Cg.PA show a reduction in EdU incorporation compared to controls.

Larvae expressing slifUY681 under the control of Scer\GAL4ppl.PP and raised to the third instar on a standard diet resemble starved wild-type larvae in that lipid droplets aggregate in the fat body. Oenocytes in third instar larvae expressing slifUY681 under the control of Scer\GAL4ppl.PP contain numerous lipid droplets, both in larvae that have been fed on a standard diet or those that have been food-deprived for 14 hours (this contrasts with wild-type larvae, where oenocytes accumulate lipid droplets specifically under starvation conditions).

Expression of slifUY681 under the control of Scer\GAL4Lsp2.PH results in an accumulation of lipid droplets in the oenocytes of fed larvae.

Colored food is present in the gut of Scer\GAL4ppl.PP>slifUY681 animals, showing that these mutants are able to swallow and process food.

When slifUY681 is driven by Scer\GAL4Bx-MS1096 a reduction in size is seen. The animals have phenotypes mimicking amino acid deprivation, exhibiting storage vesicle aggregation in the fat body. When slifUY681 is driven by Scer\GAL4ppl.PP in the fat bodies, despite normal feeding behaviour and processing of food along the digestive tract, larval development is delayed and accompanied by significant growth defects. Growth rate is strongly reduced throughout larval life. When induced at 18oC emerging adults are reduced in size and body weight (54% of wild-type). Stronger induction (at 25oC) provokes a longer developmental delay associated with pupal lethality. Unlike for wild-type flies, a 90% reduction of arginine concentration in the food leads to a dramatic aggravation of the growth defect, with most animals dying as small L2 larvae. This aggravation is not seen after starvation for valine. growth in the salivary glands (a larval endoreduplicating tissue) are less affected than growth in imaginal discs. These larvae also displayed remobilisation of lipid vesicles in fat body cells, as well as a reduction in cell size and endoreduplication levels comparable to the effect of a diet lacking protein.

Flies expressing slifUY681 under the control of Scer\GAL4en-e16E show a significant difference in growth between the posterior compartment of the wing (which expresses Scer\GAL4en-e16E) and the anterior compartment of the wing (internal control).

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Third instar larval eye-antennal imaginal disc clones co-expressing dlg1GD4689 and Ras85DV12.Scer\UAS under the control of Scer\GAL4Act5C.CoinFLP-FRT, in combination with Dicer-2 for efficient RNAi, form invasive tumors, whose size is significantly reduced by the additional clonal co-expression of slifUY681.

Expression of slifUY681 in Pten117 mutant clones results in a slight reduction of the mutant tissue under normal conditions. Under starvation conditions (10g/l yeast), these double mutant cells are almost completely eliminated. This loss of Pten117 mutant tissue is accompanied by a massive increase in apoptosis. The structure of adult eyes in these mutants are wild-type, indicating that the surrounding tissue is able to compensate for the loss of the clones.

Knockdown of Atg5, through expression of Atg5dsRNA.Scer\UAS, does not impact on the initial growth of Pten117 clones with reduced slif function (through expression of slifUY681).

Xenogenetic Interactions
Statement
Reference

Blocking apoptosis, through expression of BacA\p35Scer\UAS.cHa in Pten117 clones with diminished slif function (through expression of slifUY681), delays the collapse of the clones but they are absent from adult eyes.

Complementation and Rescue Data
Partially rescued by
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (13)