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General Information
Symbol
Dmel\RhebUAS.cSa
Species
D. melanogaster
Name
UAS construct of Saucedo
FlyBase ID
FBal0152251
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Rheb
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Expression of a XhoI/BglII fragment of a full length Rheb EST (GH10361), is under the control of UASt sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Ectopic expression of RhebScer\UAS.cSa under the Scer\GAL4elav-C155 driver increases the number of neuromuscular junction boutons in third instar larvae compared to controls.

Expression of RhebScer\UAS.cSa under the control of Scer\GAL4GMR.PU greatly increases cell size in the compound eyes of adult flies.

Expression of RhebScer\UAS.cSa under the control of Scer\GAL4en.PU results in an increase in the size of the posterior compartment of the wing relative to the anterior compartment.

Young flies expressing RhebScer\UAS.cSa in the eye under the control of Scer\GAL4GMR.PU exhibit normal eye morphology, although the ommatidium size is much larger than in controls. However, these flies undergo an age-dependent loss of photoreceptor cells when cultured on a 12-h light/12-h dark cycle. Flies cultured in continuous darkness lose photoreceptor cells more slowly than those exposed to light, indicating that photoreceptor degeneration in RhebScer\UAS.cSa-overexpressing eyes is dependent on phototransduction.

The eyes of Scer\GAL4GMR.PF driven RhebScer\UAS.cSa flies are similar in size to controls and have normal retinal morphology when the flies are young. However, few R1-R6 rhabdomeres are detected in light-exposed 30-day old flies, although R7 rhabdomeres are generally present.

Flies expressing RhebScer\UAS.cSa under the control of Scer\GAL4GMR.PF lose major photoreceptor neurons after 30 days of light/dark culture.

Expression of RhebScer\UAS.cSa under the control of Scer\GAL4en-e16E increases nucleolar size, which is an index of ribosome biogenesis.

Expression of RhebScer\UAS.cSa under the control of Scer\GAL4ptc-559.1 results in a 15% increased in the distance between wing veins L3 and L4 compared to controls.

RhebScer\UAS.cSa; Scer\GAL4GMR.PF flies have highly enlarged eyes. The wings of RhebScer\UAS.cSa; Scer\GAL4en-e16E flies have an enlarged posterior compartment (22% expansion; P < 0.0002), with minimal disruption of patterning. Wing hair density in the posterior compartment is decreased to 0.74 of wild-type, corresponding to a 34% increase in adult wing cell area (P > 0.0004). Analysis of cell size by forward scatter analysis of dissociated wing disc cells of the genotype RhebScer\UAS.cSa; Scer\GAL4Act5C.PP, shows an increase of up to 65% in mean FSC compared to wild-type. The fraction of these cells in G1 is reduced from 43% to 16% compared to wild-type. The doubling time of these cells is only slightly reduced compared to wild-type (13.7 hours vs 13.9 hours for controls). (All of the above data comes from Scer\GAL4Act5C.PP clones induced 88 hours after egg laying, and harvested/analysed as third instar larvae). In RhebScer\UAS.cSa animals, somatic clones of Scer\GAL4Act5C.PP cells in the gut, proventriculus and fat body. Such clones induced in fat body prior to endoreplication encompass approximately 2.5 times the area of control cells (P < 0.02) and contain, on average, 64% more DNA (P<0.007) when assayed in wandering, fed L3 larvae. In second instar larvae starved for 72 hours, these clone cells continue to grow, whereas surrounding wild-type cells do not.

RhebScer\UAS.cSa ; Scer\GAL4Act5C.PP clones in the wing imaginal disc attain a significantly larger size than surrounding wild-type cells. This enlargement is caused by a significant increase in cell size (a 48% increase in area covered per cell). Observation of mosaic ommatidia shows that this phenotype is cell autonomous. In contrast, the cell doubling time remains unchanged in these cells compared to control cells (10.5 h versus 10.8 h). The increase in clonal area 40 hours after induction is caused by larger cells (n = 20, P = 0.00002), whereas the number of cells is not significantly altered (P = 0.087). RhebScer\UAS.cSa ; Scer\GAL4GMR.PF animals have enlarged but fully differentiated photoreceptor cells (rhabdomere size is increased by 66%). Under normal nutrient availability, RhebScer\UAS.cSa ; Scer\GAL4Act5C.PP clone cells in the salivary gland and fat body display only a mild size increase. In larvae starved for amino acids, RhebScer\UAS.cSa ; Scer\GAL4Act5C.PP clone cells in the salivary glands display a several fold increase in size and contain much more DNA than non-expressing neighbouring cells, but they do not reach normal size and ploidy. However, RhebScer\UAS.cSa ; Scer\GAL4Act5C.PP clone cells in the fat body cells of these larvae display a complete reversion the starvation phenotype: the size, DNA content, and appearance (amount of vesicles) of these cells are indistinguishable from non-starved cells.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
NOT suppressed by
NOT Suppressor of
Other
Phenotype Manifest In
Suppressed by
NOT suppressed by
Suppressor of
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

The increased number of neuromuscular junction boutons in third instar larvae expressing RhebScer\UAS.cSa under the Scer\GAL4elav-C155 driver is significantly suppressed by combination with wnd3/+.

Lst81 does not suppress the increased cell size seen in the eyes of flies expressing RhebScer\UAS.cSa under the control of Scer\GAL4GMR.PU.

Lst81 des not suppress the overgrowth of the posterior compartment of the wing relative to the anterior compartment which is seen in flies expressing RhebScer\UAS.cSa under the control of Scer\GAL4en.PU.

Flies co-expressing TorScer\UAS.cWa, raptorScer\UAS.cWa and RhebScer\UAS.cSa under the control of Scer\GAL4GMR.PU completely lack photoreceptor cells in the eye. Evidence of cell death, such as accumulation of prominent vacuoles and highly condensed particles, is seen. This phenotype is still seen if the flies are either also co-expressing Lst8Scer\UAS.cWa or are mutant for Lst81.

Flies co-expressing TorScer\UAS.cWa, raptorScer\UAS.cWa and RhebScer\UAS.cSa under the control of Scer\GAL4GMR.PU (using the temperature sensitive Scer\GAL80ts.αTub84B allele to limit expression to 4 hours immediately following pupa formation) show a dramatic increase in ommatidial size compared to controls. This phenotype is still seen if the flies are either also co-expressing Lst8Scer\UAS.cWa or are mutant for Lst81.

Flies co-expressing TorScer\UAS.cWa, raptorScer\UAS.cWa and RhebScer\UAS.cSa under the control of Scer\GAL4en.PU (using the temperature sensitive Scer\GAL80ts.αTub84B allele to limit expression to 4 hours immediately following pupa formation) show overgrowth of the posterior compartment of the wing relative to the anterior compartment. This phenotype is still seen if the flies are either also co-expressing Lst8Scer\UAS.cWa or are mutant for Lst81.

Co-expression of Atg1ninaE.T:Hsap\MYC with Scer\GAL4GMR.PF RhebScer\UAS.cSa suppresses the neuronal degeneration found upon expression of RhebScer\UAS.cSa alone.

The distance between wing veins L3 and L4 is smaller than normal in flies co-expressing RhebScer\UAS.cSa and TctpdsRNA.Scer\UAS under the control of Scer\GAL4ptc-559.1, resembling the phenotype seen in flies expressing TctpdsRNA.Scer\UAS alone under the control of Scer\GAL4ptc-559.1.

Cells expressing RhebScer\UAS.cSa driven by Scer\GAL4Act5C.PP in a Dpa1/Dpa2 background, the compensatory elongation of the G2 phase, is not as pronounced seen in dmScer\UAS.cZa expressing cells.

The compensatory elongation of G2 phase seen in cells expressing RhebScer\UAS.cSa driven by Scer\GAL4Act5C.PP is not as pronounced in a Dpa1/Dpa2 background.

Eye overgrowth in RhebScer\UAS.cSa; Scer\GAL4GMR.PF animals is not suppressed by S6kl-1/S6kl-1, but ommatidia are smaller and less disorganized than those of RhebScer\UAS.cSa; Scer\GAL4GMR.PF alone, and the resulting eyes have a distinctive puckered appearance. The small size and increase in proportion of cells in G1 seen in cell from TorΔP homozygous wing disc clones, is unafected by the expression of RhebScer\UAS.cSa in the clone cells (driven by Scer\GAL4αTub84B.PL when Scer\GAL80αTub84B.PL is removed in the same mitotic recombination event that creates the TorΔP homozygous clone.)

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescues
Comments

With or without heat-shock, RhebScer\UAS.cSa; Scer\GAL4hs.PB partially rescues the growth defect of RhebPΔ1/RhebPΔ2 animals, enabling them to reach the second larval stage before arresting.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
RhebScer\UAS.cSa
RhebUAS.cSa
Name Synonyms
UAS construct of Saucedo
Secondary FlyBase IDs
    References (13)