FlyBase Allele Report: Dmel\Gen[Z5997]

General Information

Symbol

Dmel\Gen^Z5997

Species

D. melanogaster

Name

FlyBase ID

FBal0154852

Feature type

allele

Associated gene

Dmel\Gen

Associated Insertion(s)

Carried in Construct

Also Known As

Gen⁵⁹⁹⁷

Key Links

Genomic Maps

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Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Laurencon et al., 2004)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

delins

3L:5,150,922..5,150,927 [-]

Comment:

Deletion of ATATAC and insertion of a single G, creating a frameshift at codons 374-375.

Inserted_sequence:

(Andersen et al., 2011)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Nature of the lesion

Statement

Reference

Deletion of ATATAC and insertion of a single G, creating a frameshift at codons 374-375 (within the conserved nuclease domain).

(Andersen et al., 2011)

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

chemical sensitive

(Laurencon et al., 2004)

chemical sensitive (with Df(3L)Exel6103)

(Bellendir et al., 2017)

viable

(Laurencon et al., 2004)

Phenotype Manifest In

Detailed Description

Statement

Reference

Gen^Z5997/Df(3L)Exel6103 mutants show significant sensitivity to larval food exposure to DNA damaging agents camptothecin, methyl methanesulfonate, hydroxyurea, and mechlorethamine, and significant sensitivity to ionizing radiation, with significantly decreased adult survival as compared to wild type.

(Bellendir et al., 2017)

mus324^ZIII-4325/mus324^ZIII-5997 mutants have a similar frequency of single-strand annealing repair (SSA) compared to controls in a P{wIw.FRT} hemizygous assay to study DNA double-stranded break repair when assayed at 38^oC, but show a reduced SSA frequency compared to controls when assayed at 32^oC.

(Wei and Rong, 2007)

Mutant larvae are sensitive to nitrogen mustard and to methyl methanesulfonate (compared to control larvae).

(Laurencon et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Gen^Z5997/Df(3L)Exel6103 has chemical sensitive phenotype, enhanceable by mus81^Nhe/mus81^Nhe

(Bellendir et al., 2017)

NOT Suppressor of

Statement

Reference

Gen^Z5997 is a non-suppressor of abnormal mitotic cell cycle phenotype of Fancm⁰⁶⁹³/Df(3R)ED6058

(Kuo et al., 2014)

Other

Statement

Reference

Fancm⁰⁶⁹³/Df(3R)ED6058, Gen^Z5997 has viable phenotype

(Kuo et al., 2014)

Fancm⁰⁶⁹³/Df(3R)ED6058, Gen^Z5997, mus81^Nhe, spn-A^093A/spn-A³ has partially lethal - majority die phenotype

(Kuo et al., 2014)

Blm^N1/Blm^D2, Gen^Z5997, spn-A^093A/spn-A³ has lethal - all die during pupal stage phenotype

(Kuo et al., 2014)

Blm^N1/Blm^D2, Gen^Z5997 has lethal - all die during first instar larval stage phenotype

(Kuo et al., 2014)

Fancm⁰⁶⁹³/Df(3R)ED6058, Gen^Z5997, mus81^Nhe has lethal - all die during pharate adult stage phenotype

(Kuo et al., 2014)

Fancm⁰⁶⁹³/Df(3R)ED6058, Gen^Z5997, mus312^D1/mus312^Z1973 has lethal - all die during pharate adult stage phenotype

(Kuo et al., 2014)

Fancm⁰⁶⁹³/Df(3R)ED6058, Gen^Z5997, mus312^D1/mus312^Z1973, spn-A^093A/spn-A³ has lethal - all die during pharate adult stage phenotype

(Kuo et al., 2014)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

mus81^Nhe/mus81^Nhe exacerbates the sensitivity of Gen^Z5997/Df(3L)Exel6103 mutants to DNA damaging agents camptothecin, methyl methanesulfonate, and hydroxyurea, as well as exacerbating sensitivity to ionizing radiation, as shown by a decrease in adult survival after larval feeding with chemical agents.

(Bellendir et al., 2017)

The rate of spontaneous mitotic crossovers in Fancm⁰⁶⁹³/Df(3R)ED6058 males is not affected if animals are also mutant for Gen^Z5997.

(Kuo et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

8465

bw¹; Gen^Z5997 st¹/TM6B, Tb¹

Notes on Origin

Discoverer

The Gen^Z4325 and Gen^Z5997 alleles contain identical mutations, thus they may be two isolates of the same mutational event.

(Andersen et al., 2011)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Gen⁵⁹⁹⁷

(Kuo et al., 2014, Andersen et al., 2011)

Gen^Z5997

(Carvajal-Garcia et al., 2021, Bellendir et al., 2017, Andersen et al., 2011)

mus324^Z5997

(Andersen et al., 2011)

mus324^ZIII-5997

mus324^ZIII5997

(Wei and Rong, 2007)

Name Synonyms

Secondary FlyBase IDs

References (7)

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