Deletion of ATATAC and insertion of a single G, creating a frameshift at codons 374-375.
G
Deletion of ATATAC and insertion of a single G, creating a frameshift at codons 374-375 (within the conserved nuclease domain).
GenZ5997/Df(3L)Exel6103 mutants show significant sensitivity to larval food exposure to DNA damaging agents camptothecin, methyl methanesulfonate, hydroxyurea, and mechlorethamine, and significant sensitivity to ionizing radiation, with significantly decreased adult survival as compared to wild type.
mus324ZIII-4325/mus324ZIII-5997 mutants have a similar frequency of single-strand annealing repair (SSA) compared to controls in a P{wIw.FRT} hemizygous assay to study DNA double-stranded break repair when assayed at 38oC, but show a reduced SSA frequency compared to controls when assayed at 32oC.
Mutant larvae are sensitive to nitrogen mustard and to methyl methanesulfonate (compared to control larvae).
GenZ5997/Df(3L)Exel6103 has chemical sensitive phenotype, enhanceable by mus81Nhe/mus81Nhe
GenZ5997 is a non-suppressor of abnormal mitotic cell cycle phenotype of Fancm0693/Df(3R)ED6058
Fancm0693/Df(3R)ED6058, GenZ5997 has viable phenotype
Fancm0693/Df(3R)ED6058, GenZ5997, mus81Nhe, spn-A093A/spn-A3 has partially lethal - majority die phenotype
BlmN1/BlmD2, GenZ5997 has lethal - all die during first instar larval stage phenotype
Fancm0693/Df(3R)ED6058, GenZ5997, mus81Nhe has lethal - all die during pharate adult stage phenotype
mus81Nhe/mus81Nhe exacerbates the sensitivity of GenZ5997/Df(3L)Exel6103 mutants to DNA damaging agents camptothecin, methyl methanesulfonate, and hydroxyurea, as well as exacerbating sensitivity to ionizing radiation, as shown by a decrease in adult survival after larval feeding with chemical agents.
The rate of spontaneous mitotic crossovers in Fancm0693/Df(3R)ED6058 males is not affected if animals are also mutant for GenZ5997.