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General Information
Symbol
Dmel\GenZ5997
Species
D. melanogaster
Name
FlyBase ID
FBal0154852
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Gen5997
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
complex substitution
Comment:

Deletion of ATATAC and insertion of a single G, creating a frameshift at codons 374-375.

Inserted_sequence:

G

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Deletion of ATATAC and insertion of a single G, creating a frameshift at codons 374-375 (within the conserved nuclease domain).

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

GenZ5997/Df(3L)Exel6103 mutants show significant sensitivity to larval food exposure to DNA damaging agents camptothecin, methyl methanesulfonate, hydroxyurea, and mechlorethamine, and significant sensitivity to ionizing radiation, with significantly decreased adult survival as compared to wild type.

mus324ZIII-4325/mus324ZIII-5997 mutants have a similar frequency of single-strand annealing repair (SSA) compared to controls in a P{wIw.FRT} hemizygous assay to study DNA double-stranded break repair when assayed at 38oC, but show a reduced SSA frequency compared to controls when assayed at 32oC.

Mutant larvae are sensitive to nitrogen mustard and to methyl methanesulfonate (compared to control larvae).

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

mus81Nhe/mus81Nhe exacerbates the sensitivity of GenZ5997/Df(3L)Exel6103 mutants to DNA damaging agents camptothecin, methyl methanesulfonate, and hydroxyurea, as well as exacerbating sensitivity to ionizing radiation, as shown by a decrease in adult survival after larval feeding with chemical agents.

The rate of spontaneous mitotic crossovers in Fancm0693/Df(3R)ED6058 males is not affected if animals are also mutant for GenZ5997.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer

The GenZ4325 and GenZ5997 alleles contain identical mutations, thus they may be two isolates of the same mutational event.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (6)