FlyBase Allele Report: Dmel\l(3)mbt[GM76]

General Information

Symbol

Dmel\l(3)mbt^GM76

Species

D. melanogaster

Name

FlyBase ID

FBal0155050

Feature type

allele

Associated gene

Dmel\l(3)mbt

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Yohn et al., 2003)

Progenitor genotype

Cytology

Description

Amino acid replacement: W928term.

(Yohn et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:27,268,154..27,268,154 [-]

Nucleotide change:

G27268154A

Amino acid change:

W928term | l(3)mbt-PB

Reported amino acid change:

W928term

Comment:

G to A nucleotide change at the second or third position of the wild type Trp codon leads to a nonsense mutation (exact site of mutation unspecified). The mutation was annotated at the second base of the codon.

(Yohn et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of brain cancer

CEA

(Richter et al., 2011)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of brain cancer

is ameliorated by ex^UAS.cBa

(Richter et al., 2011)

is ameliorated by yki^B5

(Richter et al., 2011)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage

(Richter et al., 2011)

abnormal neuroanatomy | third instar larval stage (with Df(3R)D605)

(Richter et al., 2011)

abnormal size | oogenesis (with Df(3R)ED10966)

(Coux et al., 2018)

decreased cell number | late third instar larval stage (with Df(3R)ED10966)

(Coux et al., 2018)

female sterile (with Df(3R)ED10966)

(Coux et al., 2018)

increased cell death | oogenesis (with Df(3R)ED10966)

(Coux et al., 2018)

increased cell number | oogenesis (with Df(3R)ED10966)

(Coux et al., 2018)

increased cell number | oogenesis | somatic clone

(Coux et al., 2018)

lethal - all die during embryonic stage | maternal effect (with Df(3R)ED10966), with Scer\GAL4^NP1624, l(3)mbt^UASp.Tag:MYC

(Coux et al., 2018)

viable | female (with Df(3R)ED10966)

(Coux et al., 2018)

Phenotype Manifest In

basal stalk (with Df(3R)ED10966)

(Coux et al., 2018)

egg chamber (with Df(3R)ED10966)

(Coux et al., 2018)

egg chamber | somatic clone

(Coux et al., 2018)

embryonic/larval optic lobe | third instar larval stage

(Richter et al., 2011)

embryonic/larval optic lobe | third instar larval stage (with Df(3R)D605)

(Richter et al., 2011)

female germline cell | increased number (with Df(3R)ED10966)

(Coux et al., 2018)

follicle cell (with Df(3R)ED10966)

(Coux et al., 2018)

nurse cell | increased number (with Df(3R)ED10966)

(Coux et al., 2018)

nurse cell | somatic clone | increased number

(Coux et al., 2018)

oocyte | increased number (with Df(3R)ED10966)

(Coux et al., 2018)

oocyte | somatic clone | increased number

(Coux et al., 2018)

ovariole (with Df(3R)ED10966)

(Coux et al., 2018)

ovary (with Df(3R)ED10966)

(Coux et al., 2018)

primordial germ cell | germline clone | maternal effect

(Yohn et al., 2003)

somatic cell of ovariole | late third instar larval stage (with Df(3R)ED10966)

(Coux et al., 2018)

Detailed Description

Statement

Reference

l(3)mbt^GM76/Df(3R)ED10966 females do not lay eggs; ovaries are atrophied and ovarioles contain fewer individualized egg chambers than l(3)mbt^GM76 heterozygous controls; egg chambers occasionally contain apoptotic cells and are highly abnormal, showing follicle layer integrity defects, accumulation of undifferentiated germ cells (i.e. marked by branched fusomes and enclosed by follicle cells), extra nurse cells and oocytes, egg chamber fusion (e.g. occasionally there are multiple germaria connected to the same aberrant egg chamber) and accumulation of stalk cells on top of follicle cells, as compared to l(3)mbt^GM76 heterozygous controls; however, similar to controls, mutant germaria present germline stem cells adjacent to the somatic niche.

Similar to controls, l(3)mbt^GM76/Df(3R)ED10966 transheterozygous ovaries in L3 have distinct apical compartments harboring terminal filaments, as well as a medial region containing germ cells and intermingled cells; however, there is a decreased number of somatic cells in a similarly sized germ cell containing region and mutant intermingled cells fail to migrate in between the progenitor germline cells and instead surround them.

Egg chambers bearing l(3)mbt^GM76 homozygous follicle cell clones show supernumerary nurse cells or multiple oocytes.

l(3)mbt^GM76/Df(3R)ED10966 females that also express l(3)mbt^UASp.Tag:MYC under the control of Scer\GAL4^tj-NP1624 produce embryos that fail to hatch.

(Coux et al., 2018)

The volume of the optic lobe neuroepithelium is significantly increased in size in homozygous and l(3)mbt^GM76/Df(3R)D605 third instar larvae compared to wild type. Cell number and mitotic pattern within the optic lobe appears normal in homozygous first and early second instar larvae, but starting in late second instar, the mutant epithelia expand and start folding.

(Richter et al., 2011)

Embryos resulting from l(3)mbt^GM76 germ-line clones have reduced numbers of germ cells compared to wild-type embryos.

(Yohn et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

l(3)mbt^GM76/Df(3R)ED10966 has abnormal size | oogenesis phenotype, suppressible | partially by nanos^BN/nanos^L7

(Coux et al., 2018)

l(3)mbt^GM76/Df(3R)ED10966 has increased cell number | oogenesis phenotype, suppressible | partially by nanos^BN/nanos^L7

(Coux et al., 2018)

l(3)mbt^GM76 has abnormal neuroanatomy phenotype, suppressible by Df(3L)mir-ban^Δ1/Df(3L)mir-ban^Δ1

(Richter et al., 2011)

l(3)mbt^GM76 has abnormal neuroanatomy phenotype, suppressible by Scer\GAL4^C855a/ex^UAS.cBa

(Richter et al., 2011)

l(3)mbt^GM76 has abnormal neuroanatomy phenotype, suppressible by yki^B5

(Richter et al., 2011)

NOT suppressed by

Statement

Reference

l(3)mbt^GM76/Df(3R)ED10966 has increased cell death | oogenesis phenotype, non-suppressible by aub^HN2/aub^QC42

(Coux et al., 2018)

l(3)mbt^GM76/Df(3R)ED10966 has increased cell number | oogenesis phenotype, non-suppressible by aub^HN2/aub^QC42

(Coux et al., 2018)

l(3)mbt^GM76/Df(3R)ED10966 has abnormal size | oogenesis phenotype, non-suppressible | partially by nanos^BN/nanos^L7

(Coux et al., 2018)

l(3)mbt^GM76/Df(3R)ED10966 has increased cell number | oogenesis phenotype, non-suppressible by nanos^L7/nos[+]

(Coux et al., 2018)

Other

Statement

Reference

Lint-1¹, l(3)mbt[+]/l(3)mbt^GM76 has female sterile phenotype

(Coux et al., 2018)

Phenotype Manifest In

Suppressed by

Statement

Reference

l(3)mbt^GM76/Df(3R)ED10966 has ovary phenotype, suppressible | partially by nanos^BN/nanos^L7

(Coux et al., 2018)

l(3)mbt^GM76/Df(3R)ED10966 has ovariole phenotype, suppressible | partially by nanos^BN/nanos^L7

(Coux et al., 2018)

l(3)mbt^GM76/Df(3R)ED10966 has egg chamber phenotype, suppressible | partially by nanos^BN/nanos^L7

(Coux et al., 2018)

l(3)mbt^GM76/Df(3R)ED10966 has female germline cell | increased number phenotype, suppressible | partially by nanos^BN/nanos^L7

(Coux et al., 2018)

l(3)mbt^GM76/Df(3R)ED10966 has nurse cell | increased number phenotype, suppressible | partially by nanos^BN/nanos^L7

(Coux et al., 2018)

l(3)mbt^GM76/Df(3R)ED10966 has oocyte | increased number phenotype, suppressible | partially by nanos^BN/nanos^L7

(Coux et al., 2018)

l(3)mbt^GM76/Df(3R)ED10966 has follicle cell phenotype, suppressible | partially by nanos^BN/nanos^L7

(Coux et al., 2018)

l(3)mbt^GM76/Df(3R)ED10966 has ovariole phenotype, suppressible | partially by pum[+]/pum⁶⁸⁰

(Coux et al., 2018)

l(3)mbt^GM76/Df(3R)ED10966 has egg chamber phenotype, suppressible | partially by pum[+]/pum⁶⁸⁰

(Coux et al., 2018)

l(3)mbt^GM76/Df(3R)ED10966 has female germline cell | increased number phenotype, suppressible | partially by pum[+]/pum⁶⁸⁰

(Coux et al., 2018)

l(3)mbt^GM76/Df(3R)ED10966 has nurse cell | increased number phenotype, suppressible | partially by pum[+]/pum⁶⁸⁰

(Coux et al., 2018)

l(3)mbt^GM76/Df(3R)ED10966 has follicle cell phenotype, suppressible | partially by pum[+]/pum⁶⁸⁰

(Coux et al., 2018)

l(3)mbt^GM76/Df(3R)ED10966 has ovariole phenotype, suppressible | partially by pum⁶⁸⁰/pum⁶⁸⁰

(Coux et al., 2018)

l(3)mbt^GM76/Df(3R)ED10966 has egg chamber phenotype, suppressible | partially by pum⁶⁸⁰/pum⁶⁸⁰

(Coux et al., 2018)

l(3)mbt^GM76/Df(3R)ED10966 has female germline cell | increased number phenotype, suppressible | partially by pum⁶⁸⁰/pum⁶⁸⁰

(Coux et al., 2018)

l(3)mbt^GM76/Df(3R)ED10966 has nurse cell | increased number phenotype, suppressible | partially by pum⁶⁸⁰/pum⁶⁸⁰

(Coux et al., 2018)

l(3)mbt^GM76/Df(3R)ED10966 has follicle cell phenotype, suppressible | partially by pum⁶⁸⁰/pum⁶⁸⁰

(Coux et al., 2018)

l(3)mbt^GM76 has embryonic/larval optic lobe phenotype, suppressible by Df(3L)mir-ban^Δ1/Df(3L)mir-ban^Δ1

(Richter et al., 2011)

l(3)mbt^GM76 has embryonic/larval optic lobe phenotype, suppressible by Scer\GAL4^C855a/ex^UAS.cBa

(Richter et al., 2011)

l(3)mbt^GM76 has embryonic/larval optic lobe phenotype, suppressible by yki^B5

(Richter et al., 2011)

NOT suppressed by

Statement

Reference

l(3)mbt^GM76/Df(3R)ED10966 has follicle cell phenotype, non-suppressible by aub^HN2/aub^QC42

(Coux et al., 2018)

l(3)mbt^GM76/Df(3R)ED10966 has ovary phenotype, non-suppressible by nanos^L7/nos[+]

(Coux et al., 2018)

l(3)mbt^GM76/Df(3R)ED10966 has ovariole phenotype, non-suppressible by nanos^L7/nos[+]

(Coux et al., 2018)

l(3)mbt^GM76/Df(3R)ED10966 has egg chamber phenotype, non-suppressible by nanos^L7/nos[+]

(Coux et al., 2018)

l(3)mbt^GM76/Df(3R)ED10966 has female germline cell | increased number phenotype, non-suppressible by nanos^L7/nos[+]

(Coux et al., 2018)

l(3)mbt^GM76/Df(3R)ED10966 has nurse cell | increased number phenotype, non-suppressible by nanos^L7/nos[+]

(Coux et al., 2018)

l(3)mbt^GM76/Df(3R)ED10966 has oocyte | increased number phenotype, non-suppressible by nanos^L7/nos[+]

(Coux et al., 2018)

l(3)mbt^GM76/Df(3R)ED10966 has follicle cell phenotype, non-suppressible by nanos^L7/nos[+]

(Coux et al., 2018)

l(3)mbt^GM76/Df(3R)ED10966 has ovary phenotype, non-suppressible by aub^HN2/aub^QC42

(Coux et al., 2018)

l(3)mbt^GM76/Df(3R)ED10966 has ovariole phenotype, non-suppressible by aub^HN2/aub^QC42

(Coux et al., 2018)

l(3)mbt^GM76/Df(3R)ED10966 has egg chamber phenotype, non-suppressible by aub^HN2/aub^QC42

(Coux et al., 2018)

l(3)mbt^GM76/Df(3R)ED10966 has female germline cell | increased number phenotype, non-suppressible by aub^HN2/aub^QC42

(Coux et al., 2018)

l(3)mbt^GM76/Df(3R)ED10966 has nurse cell | increased number phenotype, non-suppressible by aub^HN2/aub^QC42

(Coux et al., 2018)

l(3)mbt^GM76/Df(3R)ED10966 has oocyte | increased number phenotype, non-suppressible by aub^HN2/aub^QC42

(Coux et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

The ovariole/egg chamber defects observed in l(3)mbt^GM76/Df(3R)ED10966 females are weakly and strongly suppressed by pum⁶⁸⁰ heterozygosity and homozygosity, respectively.

At 25[o]C temperature, Lint-1¹/Lint-1¹, l(3)mbt^GM76/+ females are sterile, unlike Lint-1¹/Lint-1¹ or l(3)mbt^GM76/+ females.

(Coux et al., 2018)

The increase in volume of the optic lobe neuroepithelium which is seen in l(3)mbt^GM76 homozygotes is suppressed if the larvae are also homozygous for Df(3L)ban^Δ1.

Expression of ex^Scer\UAS.cBa under the control of Scer\GAL4^C855a suppresses the increase in volume of the optic lobe neuroepithelium which is seen in l(3)mbt^GM76 homozygotes.

(Richter et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

l(3)mbt^GM76/Df(3R)ED10966 is partially rescued by l(3)mbt^UASp.Tag:MYC/Scer\GAL4^NP1624

(Coux et al., 2018)

Comments

The egg chamber phenotypes of l(3)mbt^GM76/Df(3R)ED10966 females are almost completely rescued (95%) by the expression of l(3)mbt^UASp.Tag:MYC under the control of Scer\GAL4^tj-NP1624.

(Coux et al., 2018)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

In trans Df(3R)D605 or Df(3R)mbt^PE3, l(3)mbt alleles form the following phenotypic series: l(3)mbt^GM161 < l(3)mbt^ts1 < l(3)mbt^GM79 < l(3)mbt^E2 < l(3)mbt^GM76. Phenotypes range from weak maternal-effect mitotic asynchrony to a severe mitotic block to oogenesis defects in trans-heterozygous females. Each of these phenotypes is temperature sensitive: phenotypes increase in severity as the temperature is raised from 18 to 29^oC.

(Yohn et al., 2003)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

dL(3)mbt^GM76

(Xu et al., 2017)

l(3)mbt⁷⁶

(Richter et al., 2011)

l(3)mbt^GM76

(Crain et al., 2024, Coux et al., 2018, Debruhl et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (6)

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