FlyBase Allele Report: Dmel\Acsl[8]

General Information

Symbol

Dmel\Acsl⁸

Species

D. melanogaster

Name

FlyBase ID

FBal0155128

Feature type

allele

Associated gene

Dmel\Acsl

Associated Insertion(s)

Carried in Construct

Also Known As

dAcsl⁸

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Parks, 2004.5.6)

Progenitor genotype

Cytology

Description

Amino acid numbering based on the 717aa isoform.

(Zhang et al., 2009)

This mutation effectively removes the last 33 amino acids.

(Zhang et al., 2009)

Amino acid replacement: K294E.

(Zhang et al., 2009)

Amino acid replacement: Q419R.

(Zhang et al., 2009)

Amino acid replacement: W685term.

(Zhang et al., 2009)

Amino acid replacement: ??term.

(Parks, 2004.5.6)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:8,681,505..8,681,505 [+]

Nucleotide change:

G8681505A

Amino acid change:

Reported amino acid change:

W685term

Comment:

G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation (exact site of mutation unspecified). Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Zhang et al., 2009)

point_mutation

2R:8,680,080..8,680,080 [+]

Nucleotide change:

A8680080G

Amino acid change:

Reported amino acid change:

K294E

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Zhang et al., 2009)

point_mutation

2R:8,680,456..8,680,456 [+]

Nucleotide change:

A8680456G

Amino acid change:

Reported amino acid change:

Q419R

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Zhang et al., 2009)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of non-syndromic X-linked intellectual disability 63

CEC with Acsl⁰⁵⁸⁴⁷

(Zhang et al., 2009)

CEA with Acsl⁰⁵⁸⁴⁷

(Huang et al., 2016)

model of non-syndromic X-linked intellectual disability

CEA with Acsl⁰⁵⁸⁴⁷

(Jia et al., 2019)

CEA

(Jia et al., 2019)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage (with Acsl⁰⁵⁸⁴⁷)

(Liu et al., 2014)

abnormal neuroanatomy | third instar larval stage (with Acsl^k10313)

(Zhang et al., 2009)

embryonic/larval segmentation phenotype (with Df(2R)H3E1)

(Zhang et al., 2011)

embryonic/larval segmentation phenotype | maternal effect | recessive

(Zhang et al., 2011)

lethal (with Acsl¹)

(Zhang et al., 2009)

lethal (with Acsl⁰⁵⁸⁴⁷)

(Zhang et al., 2009)

lethal | recessive

(Zhang et al., 2009)

Phenotype Manifest In

embryonic abdominal segment 1 | maternal effect

(Zhang et al., 2011)

embryonic abdominal segment 2 | maternal effect

(Zhang et al., 2011)

embryonic abdominal segment 3 | maternal effect

(Zhang et al., 2011)

embryonic abdominal segment 4 | maternal effect

(Zhang et al., 2011)

embryonic abdominal segment 5 | maternal effect

(Zhang et al., 2011)

embryonic abdominal segment 6 | maternal effect

(Zhang et al., 2011)

embryonic abdominal segment 7 | maternal effect

(Zhang et al., 2011)

embryonic abdominal segment (with Df(2R)H3E1)

(Zhang et al., 2011)

lamina anlage glial cell (with Acsl^k10313)

(Zhang et al., 2009)

lamina plexus (with Acsl^k10313)

(Zhang et al., 2009)

larval abdominal 2 neuron (with Acsl⁰⁵⁸⁴⁷)

(Liu et al., 2014)

larval abdominal 3 neuron (with Acsl⁰⁵⁸⁴⁷)

(Liu et al., 2014)

larval abdominal 4 neuron (with Acsl⁰⁵⁸⁴⁷)

(Liu et al., 2014)

larval abdominal 5 neuron (with Acsl⁰⁵⁸⁴⁷)

(Liu et al., 2014)

larval abdominal 6 neuron (with Acsl⁰⁵⁸⁴⁷)

(Liu et al., 2014)

larval abdominal 7 neuron (with Acsl⁰⁵⁸⁴⁷)

(Liu et al., 2014)

larval brain | third instar larval stage (with Acsl^k10313)

(Zhang et al., 2009)

medulla anlage (with Acsl^k10313)

(Zhang et al., 2009)

neuromuscular junction (with Acsl⁰⁵⁸⁴⁷)

(Liu et al., 2014)

NMJ bouton (with Acsl⁰⁵⁸⁴⁷)

(Liu et al., 2014)

Detailed Description

Statement

Reference

Neuromuscular junction 4 (NMJ4) terminals look similar across larval abdominal segments A3-A6 in wild type. However, in Acsl⁰⁵⁸⁴⁷/Acsl⁸ mutants, NMJs innervating the anterior segment muscles have shorter axons that are overgrown with more satellite boutons, whereas those innervating the posterior segment muscles have longer axons and are dystrophic with relatively few boutons.

(Liu et al., 2014)

About 18% of Acsl⁸/Df(2R)H3E1 mutant embryos display segmentation defects.

Among the Acsl⁸ maternal mutant embryos, approximately 59% show segmentation defects. The segments A1-A7 are most frequently affected.

(Zhang et al., 2011)

Neutral lipid accumulation is blocked in Acsl⁸/Acsl⁰⁵⁸⁴⁷ wing discs.

Total triacylglycerol content of Acsl⁸/Acsl⁰⁵⁸⁴⁷ pharates is significantly lower than wild type.

The normally defined pattern of axon projection in the optic lamina of the third instar larval brain is disrupted in the Acsl⁸/Acsl^k10313 mutant.

The following phenotypes are observed in the optic ganglia of Acsl⁸/Acsl^k10313 mutants: lamina neurons are significantly reduced in number, the normally fine alignment of glial cells disappears, the lamina plexus is in disarray, the number of glial cells in the medulla is greatly decreased, axon termination in the medulla is barely observed.

(Zhang et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Acsl⁰⁵⁸⁴⁷/Acsl⁸ has abnormal neuroanatomy | larval stage phenotype, enhanceable by Dad^j1E4/Dad[+]

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl⁸ has abnormal neuroanatomy | larval stage phenotype, enhanceable by Rab11^93Bi/Rab11[+]

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl⁸ has abnormal neuroanatomy | larval stage phenotype, enhanceable by Rab11^93Bi/Rab11^93Bi

(Liu et al., 2014)

Suppressed by

Statement

Reference

Acsl¹/Acsl⁸ has lethal phenotype, suppressible | partially by Hsap\ACSL4^{UAS.L.Tag:MYC}/Scer\GAL4^Tub.PU

(Zhang et al., 2009)

Acsl⁰⁵⁸⁴⁷/Acsl⁸ has lethal phenotype, suppressible | partially by Hsap\ACSL4^{UAS.L.Tag:MYC}/Scer\GAL4^Tub.PU

(Zhang et al., 2009)

Acsl¹/Acsl⁸ has lethal phenotype, suppressible | partially by Scer\GAL4^Tub.PU/Hsap\ACSL3^UAS.Tag:MYC

(Zhang et al., 2009)

Acsl⁰⁵⁸⁴⁷/Acsl⁸ has lethal phenotype, suppressible | partially by Scer\GAL4^Tub.PU/Hsap\ACSL3^UAS.Tag:MYC

(Zhang et al., 2009)

Acsl⁸/Acsl^k10313 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Hsap\ACSL4^{UAS.L.Tag:MYC}/Scer\GAL4^Tub.PU

(Zhang et al., 2009)

NOT suppressed by

Statement

Reference

Acsl¹/Acsl⁸ has lethal phenotype, non-suppressible by Scer\GAL4^Tub.PU/Hsap\ACSL4^{R570S.UAS.L.Tag:MYC}

(Zhang et al., 2009)

Acsl⁰⁵⁸⁴⁷/Acsl⁸ has lethal phenotype, non-suppressible by Scer\GAL4^Tub.PU/Hsap\ACSL4^{R570S.UAS.L.Tag:MYC}

(Zhang et al., 2009)

Acsl¹/Acsl⁸ has lethal phenotype, non-suppressible by Hsap\ACSL4^{P375L.UAS.L.Tag:MYC}/Scer\GAL4^Tub.PU

(Zhang et al., 2009)

Acsl⁰⁵⁸⁴⁷/Acsl⁸ has lethal phenotype, non-suppressible by Hsap\ACSL4^{P375L.UAS.L.Tag:MYC}/Scer\GAL4^Tub.PU

(Zhang et al., 2009)

Acsl⁸/Acsl^k10313 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Scer\GAL4^Tub.PU/Hsap\ACSL4^{R570S.UAS.L.Tag:MYC}

(Zhang et al., 2009)

Acsl⁸/Acsl^k10313 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Hsap\ACSL4^{P375L.UAS.L.Tag:MYC}/Scer\GAL4^Tub.PU

(Zhang et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

Acsl⁰⁵⁸⁴⁷/Acsl⁸ has neuromuscular junction phenotype, enhanceable by Dad^j1E4/Dad[+]

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl⁸ has NMJ bouton | increased number phenotype, enhanceable by Dad^j1E4/Dad[+]

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl⁸ has neuromuscular junction phenotype, enhanceable by Rab11^93Bi/Rab11[+]

(Liu et al., 2014)

Acsl⁰⁵⁸⁴⁷/Acsl⁸ has NMJ bouton | increased number phenotype, enhanceable by Rab11^93Bi/Rab11^93Bi

(Liu et al., 2014)

Suppressed by

Statement

Reference

Acsl⁸/Acsl^k10313 has larval brain | third instar larval stage phenotype, suppressible by Hsap\ACSL4^{UAS.L.Tag:MYC}/Scer\GAL4^Tub.PU

(Zhang et al., 2009)

Acsl⁰⁵⁸⁴⁷/Acsl⁸ has phenotype, suppressible by Hsap\ACSL4^{UAS.L.Tag:MYC}/Scer\GAL4^Tub.PU

(Zhang et al., 2009)

Acsl⁰⁵⁸⁴⁷/Acsl⁸ has phenotype, suppressible by Hsap\ACSL4^{UAS.S.Tag:MYC}/Scer\GAL4^Tub.PU

(Zhang et al., 2009)

Acsl⁰⁵⁸⁴⁷/Acsl⁸ has phenotype, suppressible by Scer\GAL4^Tub.PU/Hsap\ACSL3^UAS.Tag:MYC

(Zhang et al., 2009)

NOT suppressed by

Statement

Reference

Acsl⁸/Acsl^k10313 has larval brain | third instar larval stage phenotype, non-suppressible by Scer\GAL4^Tub.PU/Hsap\ACSL4^{R570S.UAS.L.Tag:MYC}

(Zhang et al., 2009)

Acsl⁸/Acsl^k10313 has larval brain | third instar larval stage phenotype, non-suppressible by Hsap\ACSL4^{P375L.UAS.L.Tag:MYC}/Scer\GAL4^Tub.PU

(Zhang et al., 2009)

Acsl⁰⁵⁸⁴⁷/Acsl⁸ has phenotype, non-suppressible by Scer\GAL4^Tub.PU/Hsap\ACSL4^{R570S.UAS.L.Tag:MYC}

(Zhang et al., 2009)

Acsl⁰⁵⁸⁴⁷/Acsl⁸ has phenotype, non-suppressible by Hsap\ACSL4^{P375L.UAS.L.Tag:MYC}/Scer\GAL4^Tub.PU

(Zhang et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Dad^j1E4/+ significantly increases synaptic overgrowth of Acsl⁰⁵⁸⁴⁷/Acsl⁸ mutants.

Rab11^93Bi/+ and, to a greater extent, Rab11^93Bi/Rab11^93Bi significantly increases neuromuscular junction overgrowth of Acsl⁰⁵⁸⁴⁷/Acsl⁸ mutants.

(Liu et al., 2014)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC} or Hsap\ACSL3^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^tub partially rescues the lethality associated with the Acsl⁸/Acsl¹ and Acsl⁸/Acsl⁰⁵⁸⁴⁷ genotypes.

Expression of Hsap\ACSL4^{R570S.Scer\UAS.L.T:Hsap\MYC} or Hsap\ACSL4^{P375L.Scer\UAS.L.T:Hsap\MYC} under the control of Scer\GAL4^tub fails to rescue the lethality associated with the Acsl⁸/Acsl¹ and Acsl⁸/Acsl⁰⁵⁸⁴⁷ genotypes.

Expression of Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC}, Hsap\ACSL4^{Scer\UAS.S.T:Hsap\MYC} or Hsap\ACSL3^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^tub restores neutral lipid staining in Acsl⁸/Acsl⁰⁵⁸⁴⁷ wing discs.

Expression of Hsap\ACSL4^{R570S.Scer\UAS.L.T:Hsap\MYC} or Hsap\ACSL4^{P375L.Scer\UAS.L.T:Hsap\MYC} under the control of Scer\GAL4^tub fails to restore neutral lipid staining in Acsl⁸/Acsl⁰⁵⁸⁴⁷ wing discs.

Expression of Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC} under the control of Scer\GAL4^tub restores normal triacylglycerol levels in Acsl⁸/Acsl⁰⁵⁸⁴⁷ wing discs.

Expression of Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC} under the control of Scer\GAL4^tub restores well-ordered axon projection in the optic lamina of the third instar larval brain of Acsl⁸/Acsl^k10313 mutants.

Expression of Hsap\ACSL4^{P375L.Scer\UAS.L.T:Hsap\MYC} or Hsap\ACSL4^{R570S.Scer\UAS.L.T:Hsap\MYC} under the control of Scer\GAL4^tub fails to restore well-ordered axon projection in the optic lamina of the third instar larval brain of Acsl⁸/Acsl^k10313 mutants.

(Zhang et al., 2009)

Complementation and Rescue Data

Rescued by

Acsl⁸/Acsl^k10313 is rescued by Acsl^{UAS.715.C.Tag:MYC}/Scer\GAL4^Tub.PU

(Zhang et al., 2009)

Acsl⁰⁵⁸⁴⁷/Acsl⁸ is rescued by Acsl^{UAS.715.C.Tag:MYC}/Scer\GAL4^Tub.PU

(Zhang et al., 2009)

Partially rescued by

Acsl¹/Acsl⁸ is partially rescued by Acsl^{UAS.715.C.Tag:MYC}/Scer\GAL4^Tub.PU

(Zhang et al., 2009)

Acsl⁰⁵⁸⁴⁷/Acsl⁸ is partially rescued by Acsl^{UAS.715.C.Tag:MYC}/Scer\GAL4^Tub.PU

(Zhang et al., 2009)

Not rescued by

Acsl⁸/Acsl^k10313 is not rescued by Acsl^{UAS.715.C.Tag:MYC}/Scer\GAL4^dpp.blk1

(Zhang et al., 2009)

Comments

Expression of Acsl^{Scer\UAS.715.T:Hsap\MYC.C} under the control of Scer\GAL4^tub partially rescues the lethality associated with the Acsl⁸/Acsl¹ and Acsl⁸/Acsl⁰⁵⁸⁴⁷ genotypes.

Expression of Acsl^{Scer\UAS.715.T:Hsap\MYC.C} under the control of Scer\GAL4^tub restores neutral lipid staining in Acsl⁸/Acsl⁰⁵⁸⁴⁷ wing discs.

Expression of Acsl^{Scer\UAS.715.T:Hsap\MYC.C} under the control of Scer\GAL4^tub restores well-ordered axon projection in the optic lamina of the third instar larval brain of Acsl⁸/Acsl^k10313 mutants.

Expression of Acsl^{Scer\UAS.715.T:Hsap\MYC.C} under the control of Scer\GAL4^dpp.blk1 fails to restore well-ordered axon projection in the optic lamina of the third instar larval brain of Acsl⁸/Acsl^k10313 mutants.

(Zhang et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

5496

Acsl⁸/CyO

8188

P{neoFRT}42D Acsl⁸ c¹ px¹ speck¹/CyO

Notes on Origin

Discoverer

Comments

Comparing Acsl⁸, Acsl¹, Acsl^k10313 and Acsl⁰⁵⁸⁴⁷, Acsl⁸ is the strongest mutation based on its lethal stage.

(Zhang et al., 2009)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Acsl⁸

(Jia et al., 2019, Huang et al., 2016)

dAcsl⁸

(Zhang et al., 2011, Zhang et al., 2009)

l(2)44DEa⁸

sAcsl⁸

(Liu et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (6)

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