brat2L-150-11/brat2L-192-9 larvae show an increase in total bouton number and in number of satellite boutons at the neuromuscular junction compared to wild type.
The mean EJP amplitude and mEJP frequency is not significantly different from wild type at the neuromuscular junction of brat2L-150-11/brat2L-192-9 larvae. However, mean mEJP amplitude is increased compared to wild type.
The neuromuscular junctions of brat2L-150-11/brat2L-192-9 larvae show reduced uptake of FM1-43 dye compared to wild type, indicating a defect in endocytosis.
Stage 15 maternal/zygotic brat2L-150-11 mutant embryos show loss of RP2 neurons. This phenotype is not seen in zygotic brat2L-150-11 embryos.
Beginning in the mid-third larval instar, the proliferation of central brain neuroblasts increases in the posterior half of the brain. By the late-third instar, these neuroblasts have essentially overgrown the entire brain and this excessive proliferation is at the expense of ganglion mother cells and neurons.
brat2L-150-11 neuroblast clones are more variable in size than wild-type clones; instead of containing one large and many small cells, brat2L-150-11 clones have more large cells.
In brat2L-150-11 mutant brains, all cells have an enlarged nucleolus, while this only applies to neuroblasts in wild-type brains.
Embryos derived from females carrying homozygous germ line clones have posterior defects.