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General Information
Symbol
Dmel\brat2L-150-11
Species
D. melanogaster
Name
FlyBase ID
FBal0155552
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
brat150
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

C19170921T

Amino acid change:

Q926term | brat-PA; Q926term | brat-PB; Q926term | brat-PC; Q926term | brat-PE; Q926term | brat-PF

Reported amino acid change:

Q926term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: Q926term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

brat2L-150-11/brat2L-192-9 larvae show an increase in total bouton number and in number of satellite boutons at the neuromuscular junction compared to wild type.

The mean EJP amplitude and mEJP frequency is not significantly different from wild type at the neuromuscular junction of brat2L-150-11/brat2L-192-9 larvae. However, mean mEJP amplitude is increased compared to wild type.

The neuromuscular junctions of brat2L-150-11/brat2L-192-9 larvae show reduced uptake of FM1-43 dye compared to wild type, indicating a defect in endocytosis.

Stage 15 maternal/zygotic brat2L-150-11 mutant embryos show loss of RP2 neurons. This phenotype is not seen in zygotic brat2L-150-11 embryos.

Beginning in the mid-third larval instar, the proliferation of central brain neuroblasts increases in the posterior half of the brain. By the late-third instar, these neuroblasts have essentially overgrown the entire brain and this excessive proliferation is at the expense of ganglion mother cells and neurons.

brat2L-150-11 neuroblast clones are more variable in size than wild-type clones; instead of containing one large and many small cells, brat2L-150-11 clones have more large cells.

In brat2L-150-11 mutant brains, all cells have an enlarged nucleolus, while this only applies to neuroblasts in wild-type brains.

Embryos derived from females carrying homozygous germ line clones have posterior defects.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

In brat2L-150-11, pros17 mutant embryos, the CNS phenotype is enhanced compared to either single mutant. In addition to the loss of neurons seen in the single mutants, the EL neurons are missing and there is a severe reduction in axon formation.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (8)