Flies expressing SoddsRNA.Scer\UAS.cHa under the control of Scer\GAL4da.G32 are short lived and highly sensitive to paraquat. However, lifespan changes in response to diet in males, but not in females, expressing SoddsRNA.Scer\UAS.cHa differ from that of control flies. Diet containing relative high sugar and low protein levels (HS-LP) results in a slight increase (16.5%) in mean lifespan of SoddsRNA.Scer\UAS.cHa-expressing males relative to the base diet, and decreased lifespan by 49.6% relative to a low sugar, low protein diet (L-C). In contrast, compared with the base diet, wild-type flies show an increase in mean lifespan in response to both HS-LP and L-C.
Antioxidant treatment in the form of N-acetylcysteine (NAC) improves the survival of males expressing SoddsRNA.Scer\UAS.cHa under the control of Scer\GAL4da.G32.
Compared with wild-type, expression of SoddsRNA.Scer\UAS.cHa using a gut-specific driver (Scer\GAL4cad-md509) is not sufficient to alter the lifespan of flies responding to diet.
Lifespan patterns in control males and females without RU486 induction of SoddsRNA.Scer\UAS.cHa expression using Scer\GAL4da.Switch.PT in response to diet are similar to that of wild-type (Canton S). The HS-LP diet shortened lifespan in males with adult-onset SoddsRNA.Scer\UAS.cHa-expression relative to the L-C diet and slightly to the base diet. However, the HS-LP diet shortened lifespan in females with adult-onset SoddsRNA.Scer\UAS.cHa-expression relative to the L-C diet only.
In wild-type males, rapamycin treatment slightly increases the lifespan of animals fed the base diet and decreases the lifespan of animals fed the L-C diet, but it does not alter lifespan in males fed the HS-LP diet, relative to diet-matched non-supplemented controls. Rapamycin increases lifespan in control females fed the base and L-C dites but not the HS-LP diet. In contrast, rapamycin increases lifespan both in males and females expressing Scer\GAL4da.G32>SoddsRNA.Scer\UAS.cHa on all three diets relative to genotype-matched non-supplemented controls. Rapamycin-fed Scer\GAL4da.G32>SoddsRNA.Scer\UAS.cHa males on the L-C diet are still longest lived relative to those on the base and HS-LP diets.
The expression of Sod1dsRNA.Scer\UAS.cHa under the control of Scer\GAL4da.G32 results in a severe decrease in life span, as compared to controls. Similar to the findings in wild-type flies, food-supplementation with up to 4% nectarine does not consistently extend the mean and maximum life span of males expressing Sod1dsRNA.Scer\UAS.cHa under the control of Scer\GAL4da.G32. However, supplementation with nectarine at 2 or 4% significantly increases the mean and maximumlife span of females expressing Sod1dsRNA.Scer\UAS.cHa under the control of Scer\GAL4da.G32.
Chronic hypoxia (5% O[]) dramatically restores the abbreviated life span of flies expressing SoddsRNA.Scer\UAS.cHa under the control of Scer\GAL4da.G32.
Animals expressing SoddsRNA.Scer\UAS.cHa under the control of Scer\GAL4da.G32 which are switched from normoxia into hypoxia at a point at which approximately 50% of the population have died continue to die as if they have remained in normoxia throughout.