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General Information
Symbol
Dmel\Sod1dsRNA.UAS.cHa
Species
D. melanogaster
Name
FlyBase ID
FBal0155800
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Sod1IR, UAS-SOD1-IR
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of an inverted repeat of Sod1 sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Sod1dsRNA.Scer\UAS.cHa RNAi under the control of Scer\GAL4da.PU strongly reduces the survival rate of adult flies compared to controls.

Expression via P{GAL4-tim.E}27 or P{cry-GAL4.Z}17 (in the presence of Dcr-2Scer\UAS.cDa) does not significantly affect circadian rhythmicity in locomotor activity assays in constant darkness.

Flies expressing SoddsRNA.Scer\UAS.cHa under the control of Scer\GAL4Hand.ΔVM.Switch show no significant difference in heart function compared to controls.

Flies expressing SoddsRNA.Scer\UAS.cHa under the control of Scer\GAL4da.G32 are short lived and highly sensitive to paraquat. However, lifespan changes in response to diet in males, but not in females, expressing SoddsRNA.Scer\UAS.cHa differ from that of control flies. Diet containing relative high sugar and low protein levels (HS-LP) results in a slight increase (16.5%) in mean lifespan of SoddsRNA.Scer\UAS.cHa-expressing males relative to the base diet, and decreased lifespan by 49.6% relative to a low sugar, low protein diet (L-C). In contrast, compared with the base diet, wild-type flies show an increase in mean lifespan in response to both HS-LP and L-C.

Antioxidant treatment in the form of N-acetylcysteine (NAC) improves the survival of males expressing SoddsRNA.Scer\UAS.cHa under the control of Scer\GAL4da.G32.

Compared with wild-type, expression of SoddsRNA.Scer\UAS.cHa using a gut-specific driver (Scer\GAL4cad-md509) is not sufficient to alter the lifespan of flies responding to diet.

Lifespan patterns in control males and females without RU486 induction of SoddsRNA.Scer\UAS.cHa expression using Scer\GAL4da.Switch.PT in response to diet are similar to that of wild-type (Canton S). The HS-LP diet shortened lifespan in males with adult-onset SoddsRNA.Scer\UAS.cHa-expression relative to the L-C diet and slightly to the base diet. However, the HS-LP diet shortened lifespan in females with adult-onset SoddsRNA.Scer\UAS.cHa-expression relative to the L-C diet only.

In wild-type males, rapamycin treatment slightly increases the lifespan of animals fed the base diet and decreases the lifespan of animals fed the L-C diet, but it does not alter lifespan in males fed the HS-LP diet, relative to diet-matched non-supplemented controls. Rapamycin increases lifespan in control females fed the base and L-C dites but not the HS-LP diet. In contrast, rapamycin increases lifespan both in males and females expressing Scer\GAL4da.G32>SoddsRNA.Scer\UAS.cHa on all three diets relative to genotype-matched non-supplemented controls. Rapamycin-fed Scer\GAL4da.G32>SoddsRNA.Scer\UAS.cHa males on the L-C diet are still longest lived relative to those on the base and HS-LP diets.

The expression of Sod1dsRNA.Scer\UAS.cHa under the control of Scer\GAL4da.G32 results in a severe decrease in life span, as compared to controls. Similar to the findings in wild-type flies, food-supplementation with up to 4% nectarine does not consistently extend the mean and maximum life span of males expressing Sod1dsRNA.Scer\UAS.cHa under the control of Scer\GAL4da.G32. However, supplementation with nectarine at 2 or 4% significantly increases the mean and maximumlife span of females expressing Sod1dsRNA.Scer\UAS.cHa under the control of Scer\GAL4da.G32.

Expression of SoddsRNA.Scer\UAS.cHa under the control of Scer\GAL4da.G32 severely sensitises flies to iron toxicity.

The expression of Sod1dsRNA.Scer\UAS.cHa under the control of Scer\GAL4da.G32 leads a significant reduction in adult lifespan, as compared to controls.

Chronic hypoxia (5% O[[2]]) dramatically restores the abbreviated life span of flies expressing SoddsRNA.Scer\UAS.cHa under the control of Scer\GAL4da.G32.

Animals expressing SoddsRNA.Scer\UAS.cHa under the control of Scer\GAL4da.G32 which are switched from normoxia into hypoxia at a point at which approximately 50% of the population have died continue to die as if they have remained in normoxia throughout.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

Expression of SoddsRNA.Scer\UAS.cHa suppresses the loss of bristle phenotype seen when Vap-33AScer\UAS.cRa is expressed under the control of Scer\GAL4sca.PU.

Xenogenetic Interactions
Statement
Reference

Overexpression of SoddsRNA.Scer\UAS.cHa suppresses the decreased survival phenotype of flies expressing Hsap\APPArctic.Scer\UAS.T:nec under the control of Scer\GAL4elav-C155.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Sod1dsRNA.Scer\UAS.cHa
Sod1dsRNA.UAS.cHa
SoddsRNA.Scer\UAS.cHa
Name Synonyms
Secondary FlyBase IDs
    References (16)