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General Information
Symbol
Dmel\Itprsv35
Species
D. melanogaster
Name
FlyBase ID
FBal0156343
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Itp-r83Asv35
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

C5534517T

Amino acid change:

Q1569term | Itp-r83A-PA; Q1578term | Itp-r83A-PB; Q1569term | Itp-r83A-PC

Reported amino acid change:

Q1572term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: Q1572term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Itp-r83Asv35 does not extend the lifespan of wild-type flies.

Itp-r83Asv35/Itp-r83Aug3 third instar larvae are significantly reduced in body size and fat body content.

The majority of Itp-r83Asv35/Itp-r83Aug3 mutant larvae ingest no or very little food compared to wild-type larvae, and defects in feeding behaviour are more prevalent in animals 108 hours after egg laying compared to 60 hours after egg laying.

There is no significant difference in the total number of salivary gland cells from Itp-r83Asv35/Itp-r83Aug3 larvae compared to wild-type larvae at 60 hours after egg laying.

Expression of Itp-r83AScer\UAS.cVa under the control of Scer\GAL4Ilp2.PR in Itp-r83Asv35/Itp-r83Aug3 flies results in significantly larger pupal size compared to wild-type pupae, and adult flies are also increased in size. There is a significant increase in the time taken to pupate by mutant larvae compared to wild-type.

Expression of Itp-r83AScer\UAS.cVa under the control of Scer\GAL4Ddc.PL in Itp-r83Asv35/Itp-r83Aug3 flies results in increased pupal length, body size, and adult fly weights. There is a significant increase in the time taken to pupate by mutant larvae compared to wild-type.

Itp-r83Asv35/Itp-r83Aug3 animals expressing Itp-r83AScer\UAS.cVa under the control of Scer\GAL412.1 and either Scer\GAL4Ilp2.PR or Scer\GAL4Ddc.PL have more surviving pupae, and pupate 12-20 hours earlier than when expression is driven by Scer\GAL4Ilp2.PR or Scer\GAL4Ddc.PL alone. Pupal and adult fly sizes in mutant animals expressing Itp-r83Asv35/Itp-r83Aug3 animals expressing Itp-r83AScer\UAS.cVa under the control of Scer\GAL412.1 and either Scer\GAL4Ilp2.PR or Scer\GAL4Ddc.PL are comparable to that of wild-type animals.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Itp-r83Asv35 partially suppresses the climbing defect of 15-day old Scer\GAL4Toll-6-D42>Hsap\TARDBPScer\UAS.cHa flies and extends their median lifespan by 5.5days.

Xenogenetic Interactions
Statement
Reference

Itp-r83Asv35 partially suppresses the climbing defect of 15-day old Scer\GAL4D42>Hsap\TARDBPScer\UAS.cHa flies.

Itp-r83Asv35 extends the median survival of Scer\GAL4D42>Hsap\TARDBPScer\UAS.cHa flies by 5.5 days. Itp-r83Asv35 does not extend the lifespan of wild-type flies.

Complementation and Rescue Data
Comments

Expression of Itp-r83AScer\UAS.cVa under the control of Scer\GAL4Ilp2.PR in Itp-r83Asv35/Itp-r83Aug3 mutant larvae significantly rescues reduced larval body size. Approximately half the surviving larvae can pupate and emerge as adults.

Expression of Itp-r83AScer\UAS.cVa under the control of Scer\GAL4Ddc.PL in Itp-r83Asv35/Itp-r83Aug3 mutant larvae significantly rescues reduced larval body size and lethality.

Expression of Itp-r83AScer\UAS.cVa under the control of Scer\GAL4Ddc.PL and Scer\GAL4Ilp2.PR in Itp-r83Asv35/Itp-r83Aug3 mutant larvae rescues body size and lethality. Survival is not improved beyond that observed with over-expression under the control of only Scer\GAL4Ddc.PL alone.

Defective feeding behaviour in Itp-r83Asv35/Itp-r83Aug3 larvae can be rescued by expression of Itp-r83AScer\UAS.cVa under the ontrol of Scer\GAL4Ilp2.PR or Scer\GAL4Ddc.PL.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (10)