FlyBase Allele Report: Dmel\Itpr[sv35]

General Information

Symbol

Dmel\Itpr^sv35

Species

D. melanogaster

Name

FlyBase ID

FBal0156343

Feature type

allele

Associated gene

Dmel\Itpr

Associated Insertion(s)

Carried in Construct

Also Known As

Itp-r83A^sv35

Genomic Maps

GBrowse JBrowse

Help me choose

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Joshi et al., 2004)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

point mutation

3R:5,534,517..5,534,517 [+]

Nucleotide change:

C5534517T

Amino acid change:

Q1569term | Itp-r83A-PA; Q1578term | Itp-r83A-PB; Q1569term | Itp-r83A-PC

Reported amino acid change:

Q1572term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Joshi et al., 2004)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Nature of the lesion

Statement

Reference

Amino acid replacement: Q1572term.

(Joshi et al., 2004)

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates amyotrophic lateral sclerosis

modeled by Hsap\TARDBP^UAS.cHa

(Kim et al., 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

body size defective (with Itpr^ug3), with Itpr^UAS.cVa, Scer\GAL4^Ddc.PL

(Agrawal et al., 2009)

body size defective (with Itpr^ug3), with Itpr^UAS.cVa, Scer\GAL4^Ilp2.PR

(Agrawal et al., 2009)

body size defective | third instar larval stage (with Itpr^ug3)

(Agrawal et al., 2009)

feeding behavior defective | third instar larval stage (with Itpr^ug3)

(Agrawal et al., 2009)

lethal - all die before end of larval stage (with Itpr^ug3)

(Agrawal et al., 2009)

some die during larval stage (with Itpr^ug3)

(Agrawal et al., 2009)

Phenotype Manifest In

embryonic/larval fat body | third instar larval stage (with Itpr^ug3)

(Agrawal et al., 2009)

Detailed Description

Statement

Reference

Itp-r83A^sv35 does not extend the lifespan of wild-type flies.

(Kim et al., 2012)

Itp-r83A^sv35/Itp-r83A^ug3 third instar larvae are significantly reduced in body size and fat body content.

The majority of Itp-r83A^sv35/Itp-r83A^ug3 mutant larvae ingest no or very little food compared to wild-type larvae, and defects in feeding behaviour are more prevalent in animals 108 hours after egg laying compared to 60 hours after egg laying.

There is no significant difference in the total number of salivary gland cells from Itp-r83A^sv35/Itp-r83A^ug3 larvae compared to wild-type larvae at 60 hours after egg laying.

Expression of Itp-r83A^Scer\UAS.cVa under the control of Scer\GAL4^Ilp2.PR in Itp-r83A^sv35/Itp-r83A^ug3 flies results in significantly larger pupal size compared to wild-type pupae, and adult flies are also increased in size. There is a significant increase in the time taken to pupate by mutant larvae compared to wild-type.

Expression of Itp-r83A^Scer\UAS.cVa under the control of Scer\GAL4^Ddc.PL in Itp-r83A^sv35/Itp-r83A^ug3 flies results in increased pupal length, body size, and adult fly weights. There is a significant increase in the time taken to pupate by mutant larvae compared to wild-type.

Itp-r83A^sv35/Itp-r83A^ug3 animals expressing Itp-r83A^Scer\UAS.cVa under the control of Scer\GAL4^12.1 and either Scer\GAL4^Ilp2.PR or Scer\GAL4^Ddc.PL have more surviving pupae, and pupate 12-20 hours earlier than when expression is driven by Scer\GAL4^Ilp2.PR or Scer\GAL4^Ddc.PL alone. Pupal and adult fly sizes in mutant animals expressing Itp-r83A^sv35/Itp-r83A^ug3 animals expressing Itp-r83A^Scer\UAS.cVa under the control of Scer\GAL4^12.1 and either Scer\GAL4^Ilp2.PR or Scer\GAL4^Ddc.PL are comparable to that of wild-type animals.

(Agrawal et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Itpr^sv35 is a suppressor | partially of short lived phenotype of Hsap\TARDBP^UAS.cHa, Scer\GAL4^Toll-6-D42

(Kim et al., 2012)

Itpr^sv35 is a suppressor | partially of locomotor behavior defective | adult stage phenotype of Hsap\TARDBP^UAS.cHa, Scer\GAL4^Toll-6-D42

(Kim et al., 2012)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Itp-r83A^sv35 partially suppresses the climbing defect of 15-day old Scer\GAL4^Toll-6-D42>Hsap\TARDBP^Scer\UAS.cHa flies and extends their median lifespan by 5.5days.

(Kim et al., 2012)

Xenogenetic Interactions

Statement

Reference

Itp-r83A^sv35 partially suppresses the climbing defect of 15-day old Scer\GAL4^D42>Hsap\TARDBP^Scer\UAS.cHa flies.

Itp-r83A^sv35 extends the median survival of Scer\GAL4^D42>Hsap\TARDBP^Scer\UAS.cHa flies by 5.5 days. Itp-r83A^sv35 does not extend the lifespan of wild-type flies.

(Kim et al., 2012)

Complementation and Rescue Data

Complements

Itpr⁰⁵⁶¹⁶

(Joshi et al., 2004)

Itpr^XR12

(Joshi et al., 2004)

Itpr^B4

(Joshi et al., 2004)

Itpr^90B.0

(Joshi et al., 2004)

Partially rescued by

Itpr^sv35 is partially rescued by Scer\GAL4^Ilp2.PR/Itpr^ug3/Itpr^UAS.cVa

(Agrawal et al., 2009)

Itpr^sv35 is partially rescued by Itpr^ug3/Scer\GAL4^Ddc.PL/Itpr^UAS.cVa

(Agrawal et al., 2009)

Itpr^sv35 is partially rescued by Scer\GAL4^Ilp2.PR/Itpr^ug3/Scer\GAL4^Ddc.PL/Itpr^UAS.cVa

(Agrawal et al., 2009)

Comments

Expression of Itp-r83A^Scer\UAS.cVa under the control of Scer\GAL4^Ilp2.PR in Itp-r83A^sv35/Itp-r83A^ug3 mutant larvae significantly rescues reduced larval body size. Approximately half the surviving larvae can pupate and emerge as adults.

Expression of Itp-r83A^Scer\UAS.cVa under the control of Scer\GAL4^Ddc.PL in Itp-r83A^sv35/Itp-r83A^ug3 mutant larvae significantly rescues reduced larval body size and lethality.

Expression of Itp-r83A^Scer\UAS.cVa under the control of Scer\GAL4^Ddc.PL and Scer\GAL4^Ilp2.PR in Itp-r83A^sv35/Itp-r83A^ug3 mutant larvae rescues body size and lethality. Survival is not improved beyond that observed with over-expression under the control of only Scer\GAL4^Ddc.PL alone.

Defective feeding behaviour in Itp-r83A^sv35/Itp-r83A^ug3 larvae can be rescued by expression of Itp-r83A^Scer\UAS.cVa under the ontrol of Scer\GAL4^Ilp2.PR or Scer\GAL4^Ddc.PL.

(Agrawal et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

30740

w^*; Itpr^sv35/TM6B, Tb¹

Notes on Origin

Discoverer

Comments

Allelic series: Itp-r83A^sv35 >= Itp-r83A^ka901 > Itp-r83A^sa54 >= Itp-r83A^ka1091 > Itp-r83A^wc703 > Itp-r83A^ug3 > Itp-r83A^wc361.

(Joshi et al., 2004)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

ITPR^SV35

(Couly et al., 2020)

Itp-r83A^sv35

(Lee et al., 2016, Kim et al., 2012)

Itpr^sv35

(Rao et al., 2016)

itpr^sv35

(Zhang et al., 2017, Chakraborty and Hasan, 2012, Agrawal et al., 2009, Kain et al., 2008)

sv35

(Joshi et al., 2004)

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

Open Close