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General Information
Symbol
Dmel\loqsf00791
Species
D. melanogaster
Name
FlyBase ID
FBal0161104
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Insertion 57bp upstream of the loqs transcription start site.

The insertion of a piggybac element within the first exon and 221 nucleotides upstream of the translational start codon of the loqs gene.

Insertion components
PBac{WH}loqsf00791
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

loqsf00791 mutant flies have a significantly shortened lifespan and show late-onset brain morphological deterioration. The brains of young adults appear normal, but by 25 days the flies have developed large vacuoles in the retina and lamina of the brain.

loqsf00791 mutants display a reduction in dsRNA processing and a subsequent ~60% reduction in siRNA levels.

The longitudinal and commissural axon structures of the central nervous system are disrupted in homozygous embryos.

Approximately 70% of homozygotes die before adulthood, and surviving males have reduced fertility and surviving females are sterile. Surviving adults are often stuck in the food.

Homozygous adults have significantly reduced locomotor activity compared to controls.

loqsf00791/loqsKO females have an average of 0.5 germline stem cells (GSCs) per germarium at 10 days after eclosion, compared to an average of 2-3 GSCs per germarium in wild-type females at this age. Differentiated cystoblasts with posterior-positioned fusomes are often seen at the normal location of GSCs in the germaria of loqsf00791/loqsKO females.

loqsf00791 mutants exhibit minimal disruption to the eye.

Only 17% of embryos derived from homozygous males mated to wild-type females hatch. Mutant males contain crystals of Ste protein in their testes. Mutant females have small ovaries and are completely sterile, failing to lay any eggs. The mutant ovarioles contain a smaller than normal germarium and contain only 2 or 3 previtellogenic egg chambers and a late-stage egg chamber (in contrast to the developmentally ordered array of 6-8 egg chambers seen in wild-type ovarioles). The mutant germarium contains only a few germ-line cells, which are not organised into distinct cysts. Spectrosomes can not be detected in the mutant germaria of 3-4 day old females, suggesting that no stem cells remain in these flies. The follicle cell layer is significantly reduced compared to wild-type. Mature oocytes in the mutant ovarioles have normal dorsal appendages.

loqsf00791 mutant females are completely sterile while males are approximately 60-70% sterile when compared with the control crosses between heterozygous and wild-type flies. Although mutant testes appear normal, mutant ovaries contain a few maturing egg chambers and a shriveled germarium with few healthy germline stem cells. The mutant ovary does not sustain continuous egg chamber production since germline stem cells can not be properly maintained.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhancer of
Suppressor of
Statement
Reference

loqsf00791 is a suppressor | partially of eye phenotype of wIR.GMR

loqsf00791/r2d21 is a suppressor | partially of eye phenotype of wIR.GMR

loqs[+]/loqsf00791 is a suppressor of male germline cell phenotype of maelM391/Df(3L)79E-F

NOT Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

The survival of roX1ex33A Df(1)roX2Δ adult males is reduced if they are also heterozygous for loqsf00791.

roX1ex40A Df(1)roX2Δ ; loqsf00791/loqsf00791 males show less than 50% survival.

loqsf00791 mutant flies carrying two copies of wIR.GMR exhibit impaired w silencing and display orange-red eyes.

loqsf00791 r2d21 double mutant flies carrying two copies of wIR.GMR exhibit impaired w silencing and display orange-red eyes.

loqsf00791 r2d21 double mutants display a reduction in dsRNA processing and a subsequent ~80% reduction in siRNA levels.

The differentiation defects seen in the testes of maelM391/Df(3L)79E-F males are partially rescued by loqsf00791/+.

The germ cell tumour phenotype seen in bamunspecified females is not suppressed by loqsf00791/loqsKO. The double mutant germaria retain germline stem cells.

Eye degeneration due to expression of Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 in the eye (under the control of Scer\GAL4GMR.PF) is enhanced in a loqsf00791 background, resulting in a severely degenerated eye with complete loss of pigmentation.

Eye degeneration due to expression of Hsap\MJDfl.Q84.Scer\UAS.T:Hsap\MYC in the eye (under the control of Scer\GAL4GMR.PF) is enhanced in a loqsf00791 background, resulting in dramatically reduced retinal thickness.

Eye degeneration due to expression of Hsap\MAPTScer\UAS.cWa in the eye (under the control of Scer\GAL4GMR.PF) is enhanced in a loqsf00791 background.

Eye degeneration due to expression of Hsap\MAPTR406W.Scer\UAS in the eye (under the control of Scer\GAL4GMR.PF) is enhanced in a loqsf00791 background.

The silencing of the w gene seen in flies carrying P{GMR-wIR} is less efficient if the flies are also homozygous for loqsf00791; flies carrying two copies of P{GMR-wIR} and homozygous for loqsf00791 do not have completely white eyes (in contrast to flies carrying two copies of P{GMR-wIR} in a wild-type background).

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer

Separable from: a second mutation on the chromosome. The loqsf00791 mutation does not cosegregate with the female sterility of the "f00791" chromosome.

Comments
Comments

Precise excision of the insertion reverts the female sterile phenotype.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
References (24)