Hemocytes trans-migrate properly from the head to tail in Rho11B mutant embryos. However, fewer of the tail hemocytes migrate anteriorly along the midline in mutants than in wild-type.
Hemocytes in Rho11B mutant embryos expressing Rho1KDQ.AAA exhibit normal head-to-tail trans-migration. However, the mutant embryos exhibit defects in hemocyte-migration from the tail. There is a significant reduction in the number of hemocytes that migrate anteriorly from the tail along the ventral midline compared with wild-type.
Hemocytes in Rho11B mutant embryos expressing Rho1F39V exhibit normal developmental migration.
The majority of salivary glands partially invaginate in homozygous embryos, but fail to turn and migrate posteriorly. Homozygous salivary gland cells observed at the stage when in wild type all gland cells have invaginated but migration has not yet started do not contract in the apical-basal axis and do not migrate dorsally, in contrast to the behaviour seen in wild-type cells at this stage.
The circular visceral mesoderm is discontinuous in homozygous embryos at a stage where it is continuous in wild-type embryos, with clusters of cells at discrete intervals along the anterior-posterior axis.
Most mutant embryos have a dilated or irregular Filzkorper, especially at the most distal part of the tube (penetrance is 75.8% in zygotic Rho11B mutants and is 69% in embryos that are both maternally and zygotically mutant for Rho11B). Approximately 4% of zygotic Rho11B mutants and 13% of embryos that are both maternally and zygotically mutant for Rho11B show a complete failure in spiracle cell invagination, with the Filzkorper forming on the surface of the embryo.
Stage 17 Rho11B/Rho172R embryos show partial disruption of the cortical actin cytoskeleton in the spiracular chamber.
Maternal mutants exhibit general endocytosis defects.
In contrast to wild type embryos, Rho11B mutant embryos fail to recruit hemocytes to a laser-induced wound after one hour: most Rho11B hemocytes in the locality of the wound are polarized toward it, but are abnormally elongated and are held back by cytoplasmic tethers. Occasionally, very elongated Rho11B cells appear to 'snap free' of their tails and leave a trail of membrane/cytoplasm behind them.
Hemocytes in Rho11B mutant embryos exhibit persistent links to one another whereas cell-cell contacts in wild type embryos are transient.