FlyBase Allele Report: Dmel\Rho1[1B]

General Information

Symbol

Dmel\Rho1^1B

Species

D. melanogaster

Name

FlyBase ID

FBal0176027

Feature type

allele

Associated gene

Dmel\Rho1

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Stramer et al., 2005)

Mutagen

P-element activity

(Magie and Parkhurst, 2005)

Progenitor genotype

Cytology

Description

The Rho1 coding region C -terminal to amino acid 52 has been removed.

(Magie and Parkhurst, 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2R:16,103,775..16,106,051 [+]

Comment:

Mutation is a deletion. Upstream breakpoint is in the first intron after amino acid 52. The distal breakpoint is unknown but extends past the coding region.

(Magie and Parkhurst, 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates hematologic cancer

modeled by hop^T42

(Bausek and Zeidler, 2014)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell migration

(Verboon et al., 2015)

abnormal cell migration, with Rho1^KDQ.AAA

(Verboon et al., 2015)

Phenotype Manifest In

circular trunk visceral muscle primordium

(Xu et al., 2008)

cortical actin cytoskeleton & spiracular chamber (with Rho1^72R)

(Simões et al., 2006)

embryonic/larval hemocyte

(Verboon et al., 2015, Stramer et al., 2005)

embryonic/larval hemocyte, with Rho1^KDQ.AAA

(Verboon et al., 2015)

filzkorper

(Simões et al., 2006)

perineurium | embryonic stage

(Li et al., 2021)

presumptive embryonic salivary gland

(Xu et al., 2008)

Detailed Description

Statement

Reference

Hemocytes trans-migrate properly from the head to tail in Rho1^1B mutant embryos. However, fewer of the tail hemocytes migrate anteriorly along the midline in mutants than in wild-type.

Hemocytes in Rho1^1B mutant embryos expressing Rho1^KDQ.AAA exhibit normal head-to-tail trans-migration. However, the mutant embryos exhibit defects in hemocyte-migration from the tail. There is a significant reduction in the number of hemocytes that migrate anteriorly from the tail along the ventral midline compared with wild-type.

Hemocytes in Rho1^1B mutant embryos expressing Rho1^F39V exhibit normal developmental migration.

(Verboon et al., 2015)

The majority of salivary glands partially invaginate in homozygous embryos, but fail to turn and migrate posteriorly. Homozygous salivary gland cells observed at the stage when in wild type all gland cells have invaginated but migration has not yet started do not contract in the apical-basal axis and do not migrate dorsally, in contrast to the behaviour seen in wild-type cells at this stage.

The circular visceral mesoderm is discontinuous in homozygous embryos at a stage where it is continuous in wild-type embryos, with clusters of cells at discrete intervals along the anterior-posterior axis.

(Xu et al., 2008)

Most mutant embryos have a dilated or irregular Filzkorper, especially at the most distal part of the tube (penetrance is 75.8% in zygotic Rho1^1B mutants and is 69% in embryos that are both maternally and zygotically mutant for Rho1^1B). Approximately 4% of zygotic Rho1^1B mutants and 13% of embryos that are both maternally and zygotically mutant for Rho1^1B show a complete failure in spiracle cell invagination, with the Filzkorper forming on the surface of the embryo.

Stage 17 Rho1^1B/Rho1^72R embryos show partial disruption of the cortical actin cytoskeleton in the spiracular chamber.

(Simões et al., 2006)

Maternal mutants exhibit general endocytosis defects.

(Magie and Parkhurst, 2005)

In contrast to wild type embryos, Rho1^1B mutant embryos fail to recruit hemocytes to a laser-induced wound after one hour: most Rho1^1B hemocytes in the locality of the wound are polarized toward it, but are abnormally elongated and are held back by cytoplasmic tethers. Occasionally, very elongated Rho1^1B cells appear to 'snap free' of their tails and leave a trail of membrane/cytoplasm behind them.

Hemocytes in Rho1^1B mutant embryos exhibit persistent links to one another whereas cell-cell contacts in wild type embryos are transient.

(Stramer et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Rho1^1B is a suppressor | partially of majority die during embryonic stage phenotype of crb^GX24w-, crb^Y10A

(Flores-Benitez and Knust, 2015)

Rho1^1B/Rho1[+] is a suppressor of melanotic mass phenotype phenotype of hop^T42

(Bausek and Zeidler, 2014)

Rho1^1B/Rho1[+] is a suppressor of decreased cell growth rate | heat sensitive phenotype of Scer\GAL4^sd-SG29.1, Src64B^UY1332

(Fernández et al., 2014)

Other

Statement

Reference

Polr2B^wimp, Rho1^1B has abnormal wound healing | embryonic stage phenotype

(Abreu-Blanco et al., 2014)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Rho1^1B is a non-enhancer of perineurium | embryonic stage phenotype of Pka-C1^B3

(Li et al., 2021)

Suppressor of

Statement

Reference

Rho1^1B is a suppressor | partially of embryo | dorsal closure stage phenotype of crb^GX24w-, crb^Y10A

(Flores-Benitez and Knust, 2015)

Rho1^1B is a suppressor | partially of embryonic/first instar larval cuticle | embryonic stage phenotype of crb^GX24w-, crb^Y10A

(Flores-Benitez and Knust, 2015)

Rho1^1B/Rho1[+] is a suppressor of melanotic mass phenotype of hop^T42

(Bausek and Zeidler, 2014)

Rho1^1B/Rho1[+] is a suppressor of wing disc phenotype of Scer\GAL4^sd-SG29.1, Src64B^UY1332

(Fernández et al., 2014)

NOT Suppressor of

Statement

Reference

Rho1^1B is a non-suppressor of perineurium | embryonic stage phenotype of Pka-C1^B3

(Li et al., 2021)

Other

Statement

Reference

Rho1^1B, wash^Δ185 has oocyte | oogenesis stage S7 phenotype

(Liu et al., 2009)

Rho1^1B, capu¹ has microtubule phenotype

(Rosales-Nieves et al., 2006)

Rho1^1B, capu¹ has oocyte | oogenesis stage S8 phenotype

(Rosales-Nieves et al., 2006)

Polr2B^wimp, Rho1^1B has microtubule phenotype

(Rosales-Nieves et al., 2006)

Polr2B^wimp, Rho1^1B has oocyte | oogenesis stage S8 phenotype

(Rosales-Nieves et al., 2006)

Rho1^1B, capu[+]/capu¹ has microtubule phenotype

(Rosales-Nieves et al., 2006)

Rho1^1B, capu[+]/capu¹ has oocyte | oogenesis stage S8 phenotype

(Rosales-Nieves et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Rho1^1B hemizygosity partially suppresses the embryonic lethality and dorsal closure defects seen in embryos expressing crb^Y10A in a crb^GX24w-/crb^GX24w- background.

(Flores-Benitez and Knust, 2015)

After single cell wounding (by laser ablation), Rho1^1B, RpII140^wimp embryos show defects in assembling an organized actin ring, display a narrowed actin halo area, and wounds expand excessively due to delayed actin recruitment. Actin cortical flow toward the wound is not disrupted.

(Abreu-Blanco et al., 2014)

Oocytes of wash^Δ185 Rho1^1B transheterozygous females undergo premature cytoplasmic streaming. The normal gradient of microtubule organisation that is seen in wild-type oocytes at stage 7 is lost in the mutant oocytes. The mutant stage 7 oocytes have disruptions in the cortical actin.

(Liu et al., 2009)

Stage 8 oocytes from capu¹/+; Rho1^1B/+ and Rho1^1B; RpII140^wimp mothers exhibit premature ooplasmic streaming and subcortical arrays of microtubules. In oocytes of both genotype, stable microtubules are restricted to the cortex, as in wild-type oocytes, but differ in the lack of reduction of microtubules at the posterior pole.

(Rosales-Nieves et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Rho1^1B is rescued by Rho1^cVa

(Verboon et al., 2015)

Rho1^1B is rescued by Rho1^+mMa

(Magie and Parkhurst, 2005)

Rho1^1B is rescued by Scer\GAL4^crq.PA/Rho1^UAS.cMa

(Stramer et al., 2005)

Partially rescued by

Rho1^1B is partially rescued by Scer\GAL4^fkh.PH/Rho1^UAS.cHa

(Xu et al., 2008)

Rho1^1B is partially rescued by Scer\GAL4^en-e16E/Rho1^UAS.cHa

(Xu et al., 2008)

Rho1^1B is partially rescued by Rho1^UAS.cHa/Scer\GAL4^wg.PM

(Xu et al., 2008)

Rho1^1B is partially rescued by Scer\GAL4^twi.PB/Rho1^UAS.cHa

(Xu et al., 2008)

Rho1^1B is partially rescued by Scer\GAL4^bap.PB/Rho1^UAS.cHa

(Xu et al., 2008)

Comments

Rho1^cVa fully rescues the maternal and zygotic phenotypes in hemocytes of Rho1^1B.

(Verboon et al., 2015)

Expression of Rho1^Scer\UAS.cHa under the control of one of Scer\GAL4^fkh.PH, Scer\GAL4^en-e16E or Scer\GAL4^wg.PM partially rescues the salivary gland migration defects of Rho1^1B embryos, with the greatest rescue being seen when expression is driven by Scer\GAL4^en-e16E.

Expression of Rho1^Scer\UAS.cHa under the control of one of either Scer\GAL4^twi.PB or Scer\GAL4^bap.PB partially rescues the salivary gland migration defects of Rho1^1B embryos.

(Xu et al., 2008)

Expression of Rho1^Scer\UAS.cMa specifically in the hemocytes (using the Scer\GAL4^crq.PA driver) resuces the hemocyte recruitment defect seen in Rho1^1B mutants.

(Stramer et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9477

w^*; Adc^b-1 pr¹ cn¹ Rho1^1B px¹ speck¹/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Rho1^1B

(Li et al., 2021, Dent et al., 2019, Qin et al., 2017, Flores-Benitez and Knust, 2015, Verboon et al., 2015, Abreu-Blanco et al., 2014, Abreu-Blanco et al., 2014, Bausek and Zeidler, 2014, Fernández et al., 2014, Liu et al., 2009, Xu et al., 2008, Simões et al., 2006, Fox et al., 2005, Stramer et al., 2005)

rho1^1B

(Simões et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (16)

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