Open Close
General Information
Symbol
Dmel\spas10-12
Species
D. melanogaster
Name
FlyBase ID
FBal0177738
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of the P{EP}spasT32 insertion has created a 560bp deletion that spans from the insertion site to partway through the first exon.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Depending on the genetic background, spas10-12/spas5.75 transheterozygous larval ddaE neurons exhibit defects in axon regeneration, shown by a significant decrease in axon length, but not in dendrite regeneration, shown by a similar dendrite number, at 96h after laser-induced severance, as compared to controls. Upon axon severance in spas10-12/spas5.75 transheterozygous larval ddaE neurons, the endoplasmic reticulum is recruited to additional neurites and less strongly to the regenerating axon, as compared to the strong accumulation only at the regenerating axon in controls. Uninjured spas10-12/spas5.75 transheterozygous neurons do not show endoplasmic reticulum defects, as compared to controls.

spas5.75 heterozygous larval ddaE neurons present an apparently normal early response to injury, as the pool of growing microtubules (identified as fluorescent EB1 comets) shown by the expected reversal of polarity at 24h after laser-induced severance, as compared to controls.

Mutant larvae have bouton numbers that do not differ significantly from controls. Mutant adults exhibit reduced flying ability. These animals climbing ability is close to wild-type. Mutant animals have an average lifespan of 35d at 25oC (compared with 46 d for wild-type controls.)

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Spas10-12
spas10-12
Name Synonyms
Secondary FlyBase IDs
    References (2)